Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Analytical Chemistry
Comprehensive Investigation Of Bioactive Steroidal Alkaloids In Veratrum Californicum, Matthew West Turner
Comprehensive Investigation Of Bioactive Steroidal Alkaloids In Veratrum Californicum, Matthew West Turner
Boise State University Theses and Dissertations
Veratrum californicum, commonly referred to as corn lily or Californian false hellebore, grows in high mountain meadows and produces bioactive steroidal alkaloids, including cyclopamine. Cyclopamine is a potent inhibitor of the Hedgehog (Hh) signaling pathway. Our lab has optimized methods to extract cyclopamine and related steroidal alkaloids from V. californicum, and implemented in-house Hh signaling bioactivity evaluation of these steroidal alkaloids using a Shh-Light II cell assay. A survey of extraction methods and solvents resulted in the identification of conditions most favorable for alkaloid extraction yield and antagonist activity in the Shh-Light II cell assay. The highest amount …
Investigating Soluble Organic Compounds In Aqueously Altered Carbonaceous Chondrites By Ultrahigh Resolution Orbitrap Mass Spectrometry, Patrick Kyle Schwartz
Investigating Soluble Organic Compounds In Aqueously Altered Carbonaceous Chondrites By Ultrahigh Resolution Orbitrap Mass Spectrometry, Patrick Kyle Schwartz
Boise State University Theses and Dissertations
Most meteorites are fragments from main-belt asteroids and contain extremely primitive materials that have preserved a compositional record of the early Solar System. After the formation of their parent bodies, carbonaceous chondrites experienced secondary modification from thermal metamorphism and/or aqueous alteration. Secondary processing likely influenced the synthesis and/or final composition of organic compounds in parent bodies; thus, these effects may be elucidated from the study of different meteorites. Although the role of aqueous alteration has been investigated for a few specific classes of compounds (such as amino acids), the effect of aqueous alteration on the full inventory of organic compounds …