Analytical Chemistry Commons^™

Chromatographic Studies Of Some Hormone And Metabolic Modulators Used For Doping In Sports, Ruth Marie Speidel

Online Theses and Dissertations

The World Anti-Doping Agency (WADA) is an international agency that monitors the use of Performance Enhancing Drugs (PEDs) in sports; their mission is “to lead a collaborative worldwide movement for doping-free sport” (Who We Are, 2022). For this mission, the WADA needs quick, sensitive, reproducible, and accurate methods for analyzing athletes’ biological fluids for possible PEDs. This research focuses on developing and validating simple, sensitive, rapid, and reliable gas chromatography- mass spectrometry (GC-MS) methods for the analysis of two hormone and metabolic modulators, toremifene and trimetazidine. Previously, there has been limited GC-MS methods used to analyze toremifene and trimetazidine. Quantification …

Absorbent Household Materials For The Collection Of Ignitable Liquid Residues From Surfaces Of Varying Porosity, Jessica Carlotti

Online Theses and Dissertations

Ignitable liquids (ILs) are used as accelerants to burn items at a faster rate and the presence of an IL indicates that arson has occurred. Upon arrival to an arson scene, fire investigators access the scene and collect substrates containing ILs. The substrates, such as concrete and wood, are removed from the scene by methods that are time consuming, and require heavy machinery and tools. The evidence is transported in air-tight containers to the laboratory where analysts identify the chemical classification of the IL using gas chromatography mass spectrometry (GC-MS). In this work, readily available absorbent household materials such as …

Development Of Novel Analytical Methods For Detection And Determination Of Tamoxifen In Oral Fluid And Discrimination Between Regioisomeric Aromatase Inhibitors For Doping Control Purposes, Brooke A. Sutton

Online Theses and Dissertations

The use of SERMs (Selective Estrogen Receptor Modulators) – most notably the drug Tamoxifen – has significantly increased among athletes over the past decade to enhance athletic performance and/or negate certain side effects of using anabolic steroids. This “doping” is always banned by WADA (World Anti-Doping Agency) for all athletes, but SERMs can naturally increase testosterone production so they are a tempting alternative to using steroids or androgenic supplements that can cause significant health problems (e.g. acne, breast development, frequent urge to urinate, low libido, etc.). Similarly, the use of AIs (Aromatase Inhibitors) has also increased among athletes for identical …

Effects Of Nutrient Overload And Environmental Conditions On Algal Bloom Formation: A Case Study Of Water Sources In Eastern Kentucky, Lauren Sutton

Online Theses and Dissertations

Eastern Kentucky is home to many private ponds used for agricultural and recreational purposes. Each year, the owners of these ponds observe harmful algal blooms (HABs) that release toxins into the water, potentially limiting the use of these ponds. A pilot study in summer 2018 observed a harmful algal bloom (HAB) occurring in one pond in Madison Country that roughly correlated with a rise in water sulfate levels with no detectable levels of nitrate or phosphate present. A follow-up study of this pond and others in the area was conducted during Summer 2019. The purpose of this project was three-fold: …

Oil Dries: A New Absorbent Material For Collection Of Ignitable Liquids From Semi-Porous Material, Jessica Sosa

Online Theses and Dissertations

Extraction of an ignitable liquid sample from a semi-porous surface such as concrete is especially difficult. For this purpose, two different solid oil dry absorbent materials, Instazorb and MAXXAbsorb, were researched for use of collection of ignitable liquid samples at a fire scene. These oil dries were spiked with different ignitable liquids (Coleman fuel, gasoline, kerosene, and diesel). Isolation of the ignitable liquid from the oil dry absorbent was accomplished by passive headspace isolation. Data was obtained by gas chromatography coupled with flame ionization detection (GC-FID) and gas chromatography coupled with mass spectrometry (GC-MS). Chromatograms obtained from both oil dries …

Latent Fingerprint Enhancement On A Brass Metal Surface Using Paint Electrodeposition, Reika D. Haskell

Online Theses and Dissertations

Fingerprint evidence recovered at a crime scene plays a key role in apprehending a suspect. Cyanoacrylate fuming is one the most successful methods of enhancing fingerprints from metallic surfaces. Despite the success of cyanoacrylate fuming, fingerprint recovery on fired cartridge casings is rarely successful. Therefore, a new fingerprint development method is needed to enhance fingerprint evidence that may still be present on fired cartridge casings. The aim of this research project is to study the use of metal and paint electrodeposition to enhance fingerprints on a brass metal surface.

High throw power paint was used in the initial study; high …

Optimization And Validation Of Direct Analysis In Real Time Mass Spectrometry (Dart-Ms) For Quantitation Of Sugars To Advance Biofuel Production, Daudi Sayialel Saang'onyo

Online Theses and Dissertations

A direct analysis in real time mass spectrometry method was developed and validated for the analysis and quantitation of sugars that would be generated from pretreated and hydrolyzed switchgrass. This research aspect can be divided into two sections:

i) Literature Review: A review is presented on the status of energy security and how biomass and biofuels can be utilized as a source of transportation fuel. To this end, biomass (specifically switchgrass) can be broken down by pretreatment methods and then enzymatically hydrolyzed to simple sugars. These sugars can assist with algae growth that can eventually be converted into biofuels. …