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Articles 1 - 5 of 5
Full-Text Articles in Analytical Chemistry
Isolation Of Proteins And Extracellular Vesicles Via Capillary-Channeled Polymer Fiber Stationary Phases, Lacey Billotto
Isolation Of Proteins And Extracellular Vesicles Via Capillary-Channeled Polymer Fiber Stationary Phases, Lacey Billotto
All Theses
Capillary-channeled polymer (C-CP) fiber stationary phases have been developed by the Marcus lab as an alternative approach to traditional, commercially-available silica-based supports for biomacromolecule separations. C-CP columns are created with natural and synthetic base polymers in a variety of chemistries and shapes, allowing for customization of separations, while providing low material costs and ease of construction. The stationary phase is virtually nonporous with respect to the size of proteins and can operate under high linear velocities (~100 mm s-1) while maintaining relatively low backpressures. As a result of these hydrodynamic benefits, low flow resistance at high volume flow …
Analyses Of Multivitamins In Nutraceuticals By Reverse Phase Hplc With Dad, Elsd And Ms, Hsiang-Chi Huang
Analyses Of Multivitamins In Nutraceuticals By Reverse Phase Hplc With Dad, Elsd And Ms, Hsiang-Chi Huang
All Theses
ABSTRACT
Vitamins are essential to human beings and support long-term healthy lifestyle. They exist widely in food, but in small amount. Hence, dietary supplements and fortified food products are more and more popular in the nutritional markets. According to the Infant Formula Act of 1980, the range of each of the nutrients should fall within a specific range or above the required minimum, so there is an increasing interest of accurate measurement of vitamins, particularly the B-group vitamins. However, traditional methods for vitamin B analyses are time-consuming and often in poor accuracy. As a result, rapid and reliable analytical methods …
Comparison Of Rna Quality From Stressed And Unstressed Recombinant Escherichia Coli, Mary Alice Salazar
Comparison Of Rna Quality From Stressed And Unstressed Recombinant Escherichia Coli, Mary Alice Salazar
All Theses
High quality, intact RNA is required for DNA microarray studies, cloning, and reverse transcriptase polymerase chain reaction (rt-PCR) analysis. There are several analytical methods used to assess the RNA quality. The RNA Integrity Number (RIN) from the Agilent Bioanalyzer is one quality control assay used to evaluate RNA. For recombinant Escherichia coli cultured under stressful conditions the RNA profiles obtained using the Agilent Bioanalyzer indicate RNA degradation; however, RNA obtained and purified in parallel from unstressed recombinant cultures indicate acceptable RNA values without significant degradation. We proposed that for stressed E. coli the RIN value is not necessarily indicative of …
Analysis Of Nutraceuticals For Toxic Metals: Development And Validation Of Sample Preparation Method, Julia Cooper
Analysis Of Nutraceuticals For Toxic Metals: Development And Validation Of Sample Preparation Method, Julia Cooper
All Theses
With increasing numbers of health-conscious consumers purchasing nutraceutical supplements, total sales in the nutrition industry have soared to over $200 billion in 2006. It is important to assess the safety of these nutraceutical products, with specific regards to toxic metals, including arsenic, cadmium, lead, and mercury. Because species of these metals may be more toxic than others, they can have hazardous health effects if ingested in excess quantities. The work described in this thesis focuses on the development of a sample preparation method for nutraceutical products, specifically ethanolic tinctures and glycerin-based matrices, and analysis of these products by inductively coupled …
Incorporation Of Capillary-Channeled Polymer (C-Cp) Fibers Into Micropipette Tips For Solid Phase Extraction With Applications In Bioanalysis, Donald Fornea
All Theses
Solid phase extraction (SPE), is a widely used sample preparation method that is currently experiencing a surge in research interest, clever architecture/format development, and stationary phase characterization.1-3 SPE devices commonly exist in syringe, cartridge, disk, and micropipette tip formats.4 Micropipette tip SPE has enhanced the determination of proteins by electrospray ionization (ESI) and matrix assisted laser desorption ionization (MALDI) mass spectrometry (MS) by handling micro-liter volumes of sample and buffer removal/solvent exchange.5-7 Buffers have deleterious effects on ionization taking place in MS sources due to preferential ionization, ion suppression effects, and the formation of adduct species competing for ionization in …