Veterinary Infectious Diseases Commons^™

Histomonas Elisa, Jessica L Martinez

EURēCA: Exhibition of Undergraduate Research and Creative Achievement

Histomonas meleagridis is a protozoan parasite of avians and is the causative agent in Histomonosis, commonly known as Blackhead Disease. Current methods for diagnosing the presence of H. meleagridis are limited to parasite culture or Polymerase Chain Reaction (PCR) to amplify target DNA. This project aims to develop an enzyme-linked immunosorbent assay (ELISA) for faster and more sensitive diagnosis of Histomonas infections. Cultures of H. meleagridis parasites were purified, and surface antigens were extracted using a spectrum of chemical solutions. The various antigen solutions were subjected to an ELISA, with serum from birds immunized for H. meleagridis as positive controls. …

Identificación De E. Coli De Cuadros Compatibles Con Colibacilosis Neonatal A Través De Pcr En Lechones De 1 Semana De Edad En 15 Granjas Porcinas En El Departamento De Antioquia, Nohora Alejandra Pabón Vega, Laura Marcela Espinel Carrasquilla

Medicina Veterinaria

La Escherichia coli enterotoxigénica, produce toxinas en el enterocito intestinal causando diarrea y muerte de los lechones de 1 semana de edad, en la cual juegan un papel importante cepa F4 (K88), generando una alta mortalidad y morbilidad de recién nacido y etapa de cría, post – destete. Jouy, Haenni, Devendec, Roux, Châtre, Madec, Kempf, (2017), generando así impactos negativos en la industria porcícola de nuestro país, es por esto que buscó detectar la presencia de E. coli enterotoxigénica a través de la técnica molecular PRC en porcinos con cuadro de diarrea, de 1 semana de edad en 15 granjas …

Cross-Reactive Immunity To Porcine Reproductive And Respiratory Syndrome Virus And Its Contribution To Protection, Ignacio Correas

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Due to the vast geographical distribution and significant economic losses generated, porcine reproductive and respiratory syndrome virus (PRRSV) can be considered the most important swine pathogen of contemporary times. Current control and eradication strategies against PRRSV have difficulty succeeding because of their complex nature and the absence of an effective vaccine. A major obstacle for PRRSV vaccine development is the broad heterogeneity of the virus, both at the genetic and antigenic level, its rapid evolution, and an incomplete knowledge of the immune responses responsible for clearing the virus from the host. Specifically, how known correlates of protection against PRRSV—neutralizing antibodies …

Developing A Plant Virus-Based Expression System For The Expression Of Vaccines Against Porcine Reproductive And Respiratory Syndrome Virus, Hong Hanh Tran

Electronic Thesis and Dissertation Repository

Virus-based expression systems have been widely exploited for the production of recombinant proteins in plants during the last three decades. Advances in technology have boosted scale-up manufacturing of plant-made pharmaceuticals to high levels, via the complementation of transient expression and viral vectors. This combination allows proteins of interest to be produced in plants within a matter of days and thus, is well suited for the development of plant-made vaccines or therapeutics against emerging infectious diseases and potential bioterrorism agents. Several plant-based products are currently in varying stages of clinical development. To investigate the viability of virus-based expression systems for plant-made …

Equine Arteritis Virus Has Specific Tropism For Stromal Cells And Cd8+ T And Cd21+ B Lymphocytes But Not For Glandular Epithelium At The Primary Site Of Persistent Infection In The Stallion Reproductive Tract, Mariano Carossino, Alan T. Loynachan, Igor F. Canisso, Richard Frank Cook, Juliana Roberta Campos, Bora Nam, Yun Young Go, Edward L. Squires, Mats H. T. Troedsson, Thomas W. Swerczek, Fabio Del Piero, Ernest F. Bailey, Peter J. Timoney, Udeni B. R. Balasuriya

Veterinary Science Faculty Publications

Equine arteritis virus (EAV) has a global impact on the equine industry as the causative agent of equine viral arteritis (EVA), a respiratory, systemic, and reproductive disease of equids. A distinctive feature of EAV infection is that it establishes long-term persistent infection in 10 to 70% of infected stallions (carriers). In these stallions, EAV is detectable only in the reproductive tract, and viral persistence occurs despite the presence of high serum neutralizing antibody titers. Carrier stallions constitute the natural reservoir of the virus as they continuously shed EAV in their semen. Although the accessory sex glands have been implicated as …

A Serosurvey Of Selected Cystogenic Coccidia In Spanish Equids: First Detection Of Anti-Besnoitia Spp. Specific Antibodies In Europe, Daniel Gutiérrez-Expósito, Ignacio García-Bocanegra, Daniel K. Howe, Antonio Arenas-Montes, Michelle R. Yeargan, Sallyanne L. Ness, Luis M Ortega-Mora, G. Álvarez-García

Veterinary Science Faculty Publications

Background: Equine besnoitiosis, caused by Besnoitia bennetti, and equine protozoal myeloencephalitis (EPM), caused by Sarcocystis neurona and Neospora hughesi are relevant equine diseases in the Americas that have been scarcely studied in Europe. Thus, a serosurvey of these cystogenic coccidia was carried out in Southern Spain. A cross-sectional study was performed and serum samples from horses (n = 553), donkeys (n = 85) and mules (n = 83) were included. An in-house enzyme-linked immunosorbent assay (ELISA) was employed to identify a Besnoitia spp. infection and positive results were confirmed by an a posteriori western blot. For Neospora …

Georgia's Collaborative Approach To Expanding Mosquito Surveillance In Response To Zika Virus: A Case Study, R. Christopher Rustin, Deonte Martin, Varadan Sevilimedu, Sarbesh Pandeya, Haresh Rochani, Rosmarie Kelly

R. Christopher Rustin

Evaluation Of Six Serological Elisa Kits Available In Italy As Screening Tests For Equine Infectious Anaemia Surveillance, Roberto Nardini, Gian Luca Autorino, Charles J. Issel, Richard F. Cook, Ida Ricci, Raffaele Frontoso, Francesca Rosone, Maria Teresa Scicluna

Maxwell H. Gluck Equine Research Center Faculty Publications

Background: ELISAs are known to have a higher diagnostic sensitivity than the agar gel immunodiffusion (AGID) when employed for serological diagnosis of equine infectious anaemia (EIA). For this purpose, an “in-house” and five commercial ELISAs available in Italy were assessed by the National Reference Centre for EIA for their analytic specificity (Sp); precocity, defined as capability of detecting first antibodies produced during a new infection; precision based on repeatability and reproducibility, estimated from the coefficient of variation (CV); accuracy, estimated from multiple K and relative Sp and sensitivity (Se). Two serum panels, positive for non-equine retroviruses and the most frequent …

Assessing Ultrasonography As A Diagnostic Tool For Porcine Cysticercosis, Robert H. Flecker, Ian W. Pray, Saul J. Santivañez, Viterbo Ayvar, Ricardo Gamboa, Claudio Muro, Luz M. Moyano, Victor Benavides, Hector H. Garcia, Seth E. O'Neal

OHSU-PSU School of Public Health Faculty Publications and Presentations

Background Taenia solium inflicts substantial neurologic disease and economic losses on rural communities in many developing nations. Ring-strategy is a control intervention that targets treatment of humans and pigs among clusters of households (rings) that surround pigs heavily infected with cysticerci. These pigs are typically identified by examining the animal's tongue for cysts. However, as prevalence decreases in intervened communities, more sensitive methods may be needed to identify these animals and to maintain control pressure. The purpose of this study was to evaluate ultrasonography as an alternative method to detect pigs heavily infected with T. solium cysts. Methodology/Principal Findings We …

Analysis Of Humoral Immune Responses In Horses With Equine Protozoal Myeloencephalitis, Catherine-Jane Angwin

Theses and Dissertations--Veterinary Science

Equine protozoal myeloencephalitis (EPM), caused by the protozoan parasite Sarcocystis neurona, is one of the most important neurological diseases of horses in the Americas. While seroprevalence of S. neurona in horses is high, clinical manifestation of EPM occurs in less than 1% of infected horses. Factors governing the occurrence and severity of EPM are largely unknown, although horse immunity might play an important role in clinical outcome. We hypothesize that EPM occurs due to an aberrant immune response, which will be discernable in the equine IgG subisotypes a, b, and (T) that recognize S. neurona in infected diseased horses versus …

Reagents And Tools To Identify Epizootic Hemorrhagic Disease Virus-2, Meghan Syrstad

Electronic Theses and Dissertations

The purpose of this project was to define monoclonal antibodies against viral proteins from Epizootic Hemorrhagic Disease Virus (EHDV) to be used in quality control of vaccines produced by an industrial partner, and development of tools to identify EHDV. EHDV is an Orbivirus, which also includes Bluetongue Virus and African Horse Sickness, which are all transmitted through arthropod vectors. EHDV causes significant morbidity and mortality in white-tailed deer, but has recently been found to infect cattle. EHDV recently caused significant outbreaks affecting both the farmed and wild cervid industry, however few reagents and tools exist to protect against this disease …

Israel : Translocation Of Rabies, Sarah Baker

Global Public Health

Israel is now faced with a steady growing public health threat as canine rabies has reemerged in the northern region of Israel. While the steady increase in cases is of notable alarm, moreover the close contact most often shared between dog and human populations is of much more concern from a public health prospective. Rabies is a vaccine-preventable disease. Vaccinating dogs has proven to be the most cost-effective strategy for preventing rabies in people. Additionally, education on bite prevention and rabid animal behaviors for both children and adults is an essential extension of the long term rabies vaccination program that …

Evaluation Of Pyriproxyfen Applied In Barrier Sprays For Mosquito Suppression, Andrea Glenn Skiles

Theses and Dissertations--Entomology

Despite advances in mosquito management, mosquito-borne disease in the United States is still of relevant public health concern and vector control is a top priority in preventing transmission of pathogens. Insecticide barrier sprays have become a common tool for suppression of mosquitoes in single-homeowner backyards. The application of the synthetic pyrethroid, lambda-cyhalothrin to perimeter vegetation with a backpack sprayer has been shown to significantly suppress mosquito levels for around 6 weeks. In an attempt to lengthen the effective duration of treatment, the IGR, pyriproxyfen, was added to a backpack mist blower with lambda-cyhalothrin, as adult mosquitoes exposed to pyriproxyfen have …

Understanding The Effect Of Bvdv On Innate Immune Response Of Neutrophils, Neelu Singh Thakur

Electronic Theses and Dissertations

Bovine viral diarrhea virus (BVDV) is one of the highly prevalent and economically important diseases of cattle industry worldwide. The two major consequences of this disease are persistent infection and immunosuppression. Several studies have been done to determine the underline mechanisms of BVDV-induced immunosuppression targeting antigen presenting cells, adaptive immune system cells and cytokine gene expression. However, very little research has been done to determine the effect of BVDV on neutrophils. Neutrophils are one of the most abundant while blood cells (WBC) in the peripheral blood, which play a critical role in the innate as well as adaptive immune response. …

Differences In Efficacy Between Gamithromycin, Tilmicosin, And Tulathromycin As Metaphylactic Treatments In High Risk Calves For Bovine Respiratory Disease, T. Miller, M. E. Hubbert, E. F. Schwandt, D. U. Thomson, C. D. Reinhardt

Kansas Agricultural Experiment Station Research Reports

The cost of Bovine Respiratory Disease to the beef industry due to death, poorer conversions, and therapy is estimated to cost more than $3 billion per year. Identifying and mitigating Bovine Respiratory Disease in cattle can be difficult due to the increased susceptibility for Bovine Respiratory Disease in high risk cattle. One management option to minimize an outbreak of respiratory disease is the use of metaphylaxis, the mass treatment of a group of calves to reduce the incidence and adverse effects of respiratory disease on high risk animals. Criteria used to determine the necessity of metaphylactic treatment against Bovine Respiratory …

Genome Diversity And Molecular Detection Of Prrs Field Strains And Vaccine Strains, And Pcv3 And Pcv2 Strains, Y. Wang, F. Yuan, X. Liu, W. Zheng, H. Zhang, J. Zhang, K. Yoon, L. Peddireddi, Y. Fang, G. Anderson, J. Bai

Kansas Agricultural Experiment Station Research Reports

Molecular diagnosis of porcine reproductive and respiratory syndrome virus (PRRS) and porcine circo virus (PCV) are challenging due to high genetic diversity in the viral genomes. Differentiating PRRS vaccine strains is even more challenging and is currently done by DNA sequencing, which is expensive and time-consuming. A multiplexed system (Luminex) allowing multiple detection targets in the same reaction is available. However, this system is not fully developed for common swine pathogens. Therefore, an assay was built to detect the majority of field PRRS strains by using different pairs of primers, and at the same time, to provide differentiation of the …

Zelnate On Arrival Could Decrease The Likelihood Of Subsequent Pulls In Suspect Bovine Respiratory Disease Complex Cases, T. Spore, S. P. Montgomery, G. A. Hanzlicek, W. R. Hollenbeck, R. N. Wahl, J. E. Sears, Dale Blasi

Kansas Agricultural Experiment Station Research Reports

Antimicrobial metaphylaxis is an important tool used for the prevention of Bovine Respiratory Disease Complex; a disease with a large economic impact that typically affects newly-weaned beef calves that are marketed and transported a distance from their origin.

There are questions involving the potential benefit of Zelnate, a novel non-antibiotic technology designed to activate an animal’s natural immunity to fight Bovine Respiratory Disease Complex, being used either solely or in combination with metaphylaxis at the time of initial processing of high risk calves. More knowledge is also needed regarding the possible effects of repeated use of Zelnate when subsequent therapy …

Identifying Immuno-Dominant And Neutralizing Epitopes From K88 Fimbriae Of Enterotoxigenic Escherichia Coli (Etec), T. Lu, W. Zhang

Kansas Agricultural Experiment Station Research Reports

Enterotoxigenic Escherichia coli (ETEC) bacteria are the primary cause of diarrheal disease, especially porcine post-weaning diarrhea (PWD). Post-weaning diarrhea is one of the most common diseases in piglets 3 to 10 days after weaning and causes the loss of millions of dollars annually to United States swine industry and other countries. These ETEC bacteria produce two types of virulence factors: 1) fimbriae adhesins, which promote bacterial attachment and colonization in pig small intestine; and 2) enterotoxins that disrupt fluid homeostasis and cause fluid hype-secretion and watery diarrhea. The F4 (K88) is the most important fimbria in ETEC bacteria causing PWD. …