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Articles 1 - 30 of 80
Full-Text Articles in Obstetrics and Gynecology
Prenatal Detection Of Congenital Heart Disease By Basic Ultrasonography At A Tertiary Care Center: What Should Our Expectations Be?, Kimberly Leslie, W. Persutte, J. Drose, R. Lenke, J. Hobbins, M. Manco-Johnson, E. Shaffer, J. Wiggins
Prenatal Detection Of Congenital Heart Disease By Basic Ultrasonography At A Tertiary Care Center: What Should Our Expectations Be?, Kimberly Leslie, W. Persutte, J. Drose, R. Lenke, J. Hobbins, M. Manco-Johnson, E. Shaffer, J. Wiggins
Kimberly K. Leslie
No abstract provided.
Maternal Serum Alpha-Fetoprotein Levels In Ectopic Pregnancies: A Prospective, Controlled Study, S. Lederman, Kimberly Leslie, Y. Lidor, R. Lenke
Maternal Serum Alpha-Fetoprotein Levels In Ectopic Pregnancies: A Prospective, Controlled Study, S. Lederman, Kimberly Leslie, Y. Lidor, R. Lenke
Kimberly K. Leslie
No abstract provided.
Vascular Endothelial Growth Factor Pathway Blockade In Endometrial Cancer, Suzy Davies, Donghai Dai, Kimberly Leslie
Vascular Endothelial Growth Factor Pathway Blockade In Endometrial Cancer, Suzy Davies, Donghai Dai, Kimberly Leslie
Kimberly K. Leslie
No abstract provided.
Induction Of Mitotic Cell Death By Overriding G2/M Checkpoint In Endometrial Cancer Cells With Non-Functional P53, Xiangbing Meng, Laura Laidler, Elizabeth Kosmacek, Shujie Yang, Zhi Xiong, Danlin Zhu, Xinjun Wang, Donghai Dai, Yuping Zhang, Xiaofang Wang, Pavia Brachova, Lina Albitar, Dawei Liu, Florenza Ianzini, Michael Mackey, Kimberly Leslie
Induction Of Mitotic Cell Death By Overriding G2/M Checkpoint In Endometrial Cancer Cells With Non-Functional P53, Xiangbing Meng, Laura Laidler, Elizabeth Kosmacek, Shujie Yang, Zhi Xiong, Danlin Zhu, Xinjun Wang, Donghai Dai, Yuping Zhang, Xiaofang Wang, Pavia Brachova, Lina Albitar, Dawei Liu, Florenza Ianzini, Michael Mackey, Kimberly Leslie
Kimberly K. Leslie
OBJECTIVE: Endometrial tumors with non-functional p53, such as serous uterine endometrial carcinomas, are aggressive malignancies with a poor outcome, yet they have an Achilles' heel: due to loss of p53 function, these tumors may be sensitive to treatments which abrogate the G2/M checkpoint. Our objective was to exploit this weakness to induce mitotic cell death using two strategies: (1) EGFR inhibitor gefitinib combined with paclitaxel to arrest cells at mitosis, or (2) BI2536, an inhibitor of polo-like kinase 1 (PLK1), to block PLK1 activity. METHODS: We examined the impact of combining gefitinib and paclitaxel or PLK1 inhibitor on expression of …
Breast Cancer And Pregnancy, Kimberly Leslie
Transposition Of The Great Vessels ,Complete And Corrected, Kimberly Leslie, E. Shaffer
Transposition Of The Great Vessels ,Complete And Corrected, Kimberly Leslie, E. Shaffer
Kimberly K. Leslie
No abstract provided.
Univentricular Heart, Kimberly Leslie, E. Shaffer
Univentricular Heart, Kimberly Leslie, E. Shaffer
Kimberly K. Leslie
No abstract provided.
Cancer Complicating Pregnancy, Kimberly Leslie
Cancer Complicating Pregnancy, Kimberly Leslie
Kimberly K. Leslie
No abstract provided.
Cancer In Pregnancy, S. Sadanandan, T. Hurley, C. Miller, C. Verschtaegen, Kimberly Leslie
Cancer In Pregnancy, S. Sadanandan, T. Hurley, C. Miller, C. Verschtaegen, Kimberly Leslie
Kimberly K. Leslie
No abstract provided.
Steroid Hormone Receptors In Normal And Malignant Endometrium, Kimberly Leslie, S. Davies, M. Singh, H. Smith
Steroid Hormone Receptors In Normal And Malignant Endometrium, Kimberly Leslie, S. Davies, M. Singh, H. Smith
Kimberly K. Leslie
No abstract provided.
Gynecological And Reproductive Health Issues (Chapter 3), S. Faro, P. Fine, Kimberly Leslie
Gynecological And Reproductive Health Issues (Chapter 3), S. Faro, P. Fine, Kimberly Leslie
Kimberly K. Leslie
No abstract provided.
Acute Intrauterine Infection Results In An Imbalance Between Pro- And Anti-Inflammatory Cytokines In The Pregnant Rabbit., Kimberly Leslie, S. Lee, S. Woodcock, J. Davies, R. Mcduffie, E. Hirsch, M. Sherman, J. Eskens, R. Gibbs
Acute Intrauterine Infection Results In An Imbalance Between Pro- And Anti-Inflammatory Cytokines In The Pregnant Rabbit., Kimberly Leslie, S. Lee, S. Woodcock, J. Davies, R. Mcduffie, E. Hirsch, M. Sherman, J. Eskens, R. Gibbs
Kimberly K. Leslie
PROBLEM: Intrauterine infection results in an increase in cytokines. This study compared the time courses for the pro- and anti-inflammatory cytokine responses in 33 pregnant rabbits at 70% gestation. Pro-inflammatory markers were activated nuclear factor-kappa B (NF-kappaB) in placenta and tumor necrosis factor-alpha (TNF-alpha) in amniotic fluid. These were compared to the anti-inflammatory cytokine, interleukin-1 receptor antagonist (IL-1ra), in placenta and uterus. METHOD OF STUDY: Does were endoscopically inoculated with Escherichia coli through their cervices and sacrificed at six intervals between 0 and 30 hr post-inoculation. RESULTS: Activated NF-kappaB, determined by electromobility gel shift assay, increased significantly 16 hr after …
Preconceptions: Preparation For Pregnancy, John Queenan, Kimberly Leslie
Preconceptions: Preparation For Pregnancy, John Queenan, Kimberly Leslie
Kimberly K. Leslie
No abstract provided.
Differential Expression Of The A And B Isoforms Of Progesterone Receptor In Human Endometrial Cancer Cells. Only Progesterone Receptor B Is Induced By Estrogen And Associated With Strong Transcriptional Activation., Kimberly Leslie, N. Kumar, J. Richer, G. Owen, G. Takimoto, K. Horwitz, C. Lange
Differential Expression Of The A And B Isoforms Of Progesterone Receptor In Human Endometrial Cancer Cells. Only Progesterone Receptor B Is Induced By Estrogen And Associated With Strong Transcriptional Activation., Kimberly Leslie, N. Kumar, J. Richer, G. Owen, G. Takimoto, K. Horwitz, C. Lange
Kimberly K. Leslie
No abstract provided.
Antenatal Testing, Kimberly Leslie
Endocrine Cancer Risks For Women During The Perimenopause And Beyond., Kimberly Leslie, N. Kumar
Endocrine Cancer Risks For Women During The Perimenopause And Beyond., Kimberly Leslie, N. Kumar
Kimberly K. Leslie
Cancer and its link to reproductive hormones is an area of intense concern for our patients and has been the subject of much speculation. But if estrogen causes breast cancer, for example, most women would eventually develop the disease. We know this is not the case! Actually, estrogen and progesterone have been linked to a decrease as well as an increase in cancer, depending upon the type of tumor under investigation. The purpose of this manuscript is to review the data supporting those relationships.
Histologic Inflammation In The Maternal And Fetal Compartments In A Rabbit Model Of Acute Intra-Amniotic Infection., J. Davies, R. Shikes, C. Sze, Kimberly Leslie, R. Mcduffie, R. Romero, R. Gibbs
Histologic Inflammation In The Maternal And Fetal Compartments In A Rabbit Model Of Acute Intra-Amniotic Infection., J. Davies, R. Shikes, C. Sze, Kimberly Leslie, R. Mcduffie, R. Romero, R. Gibbs
Kimberly K. Leslie
OBJECTIVE: This study was undertaken to determine the course of acute inflammation in the maternal and fetal compartments during experimentally induced ascending intra-amniotic infection. STUDY DESIGN: Forty pregnant rabbits at 70% gestation were inoculated endocervically with 10(5) colony-forming units of Escherichia coli. Does were killed at 0, 4, 8, 16, 24, and 30 hours after inoculation. At necropsy, blood, peritoneal fluid, amniotic fluid, and uterine tissue were cultured. Fetal brain, lung, heart, gut, and kidney were collected for histologic examination. Necrosis, infiltrates, congestion, and edema were each assessed semiquantitatively, and mean composite histologic-inflammation scores were compared with analysis of variance. …
Physiology Of Labor, Kimberly Leslie
Selective Down-Regulation Of Progesterone Receptor Isoform B In Poorly Differentiated Human Endometrial Cancer Cells: Implications For Unopposed Estrogen Action., N. Kumar, J. Richer, G. Owen, E. Litman, K. Horwitz, Kimberly Leslie
Selective Down-Regulation Of Progesterone Receptor Isoform B In Poorly Differentiated Human Endometrial Cancer Cells: Implications For Unopposed Estrogen Action., N. Kumar, J. Richer, G. Owen, E. Litman, K. Horwitz, Kimberly Leslie
Kimberly K. Leslie
The uterine endometrium responds to unopposed estrogen stimulation with rapid cell proliferation. Progesterone protects the endometrium against the hyperplastic effects of estradiol (E2) through progesterone receptors (PRs), of which two isoforms are expressed: human (h) PRA and PRB. hPRB has a longer NH2 terminus and may function differently from hPRA. Thus, the relative expression of hPRA:hPRB is likely to be important for the action of progesterone. We hypothesized that the hPRA:hPRB ratios may be abnormal in endometrial cancer, leading to a lack of normal progesterone protection against the growth-promoting effects of E2. To test this hypothesis, well-differentiated Ishikawa endometrial cancer …
Why Are Steroid Receptor Antagonists Sometimes Agonists?, Kimberly Leslie
Why Are Steroid Receptor Antagonists Sometimes Agonists?, Kimberly Leslie
Kimberly K. Leslie
No abstract provided.
Estrogens In Intrahepatic Cholestasis Of Pregnancy., Kimberly Leslie, L. Reznikov, F. Simon, P. Fennessey, H. Reyes, J. Ribalta
Estrogens In Intrahepatic Cholestasis Of Pregnancy., Kimberly Leslie, L. Reznikov, F. Simon, P. Fennessey, H. Reyes, J. Ribalta
Kimberly K. Leslie
OBJECTIVE: To determine whether estrogen production and excretion are impaired in gravidas with intrahepatic cholestasis. METHODS: Plasma and urine samples were collected from 13 women from the United States and Chile at 35-38 weeks' gestation with mild (n = 9) or severe (n = 4) intrahepatic cholestasis of pregnancy. Urinary and plasma steroid levels from women with cholestasis were compared with levels from 27 normal pregnant women within the same gestational age range. Urinary concentrations of dehydroepiandrosterone (DHEA), estrone (E1), estradiol (E2), estriol (E3), estetrol, progesterone, and 16-hydroxy-pregnenolone were measured by gas chromatography mass spectrometry, and plasma concentrations of DHEA …
Oestrogen Modulation With Parturition In The Human Placenta., Kimberly Leslie, D. Zuckerman, J. Schruefer, M. Burchell, J. Smith, B. Albertson
Oestrogen Modulation With Parturition In The Human Placenta., Kimberly Leslie, D. Zuckerman, J. Schruefer, M. Burchell, J. Smith, B. Albertson
Kimberly K. Leslie
An initial group of term (36-41 6/7 weeks), preterm (less than 36 weeks), and post-term (42 or more weeks) placentae were collected from women at delivery to determine the placental levels of important steroids and steroidogenic enzymes involved in the oestrogen synthesis pathway as a function of gestational age. A second group of placentae were obtained from women delivering at term before and after the onset of labour. Placentae were evaluated individually for cytosolic steroid hormone levels and microsomal steroidogenic enzyme activities. Oestradiol (E2), oestrone (E1), progesterone (P), and delta-4-androstenedione (A) were measured by radioimmunoassay in placental cytosols. Aromatase (AR), …
Estrogen Receptors Are Present In Human Granulosa Cells., B. Hurst, M. Zilberstein, J. Chou, B. Litman, J. Stephens, Kimberly Leslie
Estrogen Receptors Are Present In Human Granulosa Cells., B. Hurst, M. Zilberstein, J. Chou, B. Litman, J. Stephens, Kimberly Leslie
Kimberly K. Leslie
Recent studies failed to detect estrogen receptors in primate follicles. This study was initiated to determine whether estrogen receptor (ER) messenger ribonucleic acid (mRNA) is present in human granulosa cells and, further, if functional ER proteins are present. To evaluate the presence of ER, RNA from human granulosa cells obtained at the time of oocyte retrieval for assisted reproduction was extracted, and complementary DNA synthesis was performed by the reverse transcriptase-polymerase chain reaction. Oligonucleotide primers were used to amplify basepairs 570-852 in the B- and C- domains of the ER mRNA. Southern blotting was performed and confirmed that the amplified …
Estrogen Receptor Mediated Regulation Of Rat Gonadotropin Releasing Hormone (Gnrh) Promoter Activity In Hypothalamic Cells, J. Kepa, C. Neely, B. Jacobsen, D. Mcdonnell, Kimberly Leslie, M. Wierman
Estrogen Receptor Mediated Regulation Of Rat Gonadotropin Releasing Hormone (Gnrh) Promoter Activity In Hypothalamic Cells, J. Kepa, C. Neely, B. Jacobsen, D. Mcdonnell, Kimberly Leslie, M. Wierman
Kimberly K. Leslie
No abstract provided.
Estrogen, Progesterone And Cellular Mechanisms Of Vascular Control, M. White, S. Zamudio, T. Stevens, R. Tyler, J. Lindenfeld, Kimberly Leslie
Estrogen, Progesterone And Cellular Mechanisms Of Vascular Control, M. White, S. Zamudio, T. Stevens, R. Tyler, J. Lindenfeld, Kimberly Leslie
Kimberly K. Leslie
No abstract provided.
Differential Gene Expression In The Amnion, Chorion, And Trophoblast Of The Human Placenta., C. Plouzek, Kimberly Leslie, J. Stephens, J. Chou
Differential Gene Expression In The Amnion, Chorion, And Trophoblast Of The Human Placenta., C. Plouzek, Kimberly Leslie, J. Stephens, J. Chou
Kimberly K. Leslie
Human chorionic gonadotrophin (hCG), placental alkaline phosphatase (PLAP), and pregnancy-specific glycoprotein (PSG) are three major proteins produced by the trophoblast of the human placenta. Immunocytochemical studies suggest that PSG and hCG are also present in the human amnion. In this study, we examined whether amniotic and chorionic membranes were capable of expressing trophoblastic-specific genes. As previously reported, trophoblasts express high levels of hCG beta, hCG alpha, PLAP, and PSG. Both amnion and chorion were found to express PLAP and hCG beta mRNA. However, the hCG alpha transcript was expressed only by the amnion, but not by the chorion in the …
The Role Of Oestrogen In Follicular Development., B. Hurst, Kimberly Leslie
The Role Of Oestrogen In Follicular Development., B. Hurst, Kimberly Leslie
Kimberly K. Leslie
Most of our knowledge of ovarian physiology is based upon studies that have demonstrated functional oestrogen receptors in the ovaries of lower animal species. The presence of oestrogen receptors in primate granulosa cells has been questioned by some investigators. However, we have found oestrogen receptor messenger RNA in human granulosa cells by reverse transcriptase-PCR assay. Furthermore, using immortalized granulosa cell lines transfected with a plasmid containing an oestrogen response element, a functional oestrogen receptor was confirmed. These experiments strongly support the hypothesis that human granulosa cells express biologically active oestrogen receptor.
Low Serum Estradiol And High Serum Progesterone Concentrations Characterize Hypertensive Pregnancies At High Altitude., S. Zamudio, Kimberly Leslie, M. White, D. Hagerman, L. Moore
Low Serum Estradiol And High Serum Progesterone Concentrations Characterize Hypertensive Pregnancies At High Altitude., S. Zamudio, Kimberly Leslie, M. White, D. Hagerman, L. Moore
Kimberly K. Leslie
OBJECTIVE: Intrauterine growth retardation and preeclampsia are more common at high than at low altitude. Because altered hormonal profiles have been linked with these disorders, we asked whether placental steroid hormone concentrations were altered during pregnancy at high altitude. METHODS: We measured progesterone, unconjugated estradiol, and estriol (by radioimmunoassay) at weeks 20, 30, and 36 of pregnancy in 18 women at low altitude (1600 m) and 40 women at high altitude (3100 m). RESULTS: Women at 3100 m compared with 1600 m had lower serum estradiol concentrations at week 36 of pregnancy, and lower estriol and higher progesterone concentrations throughout …
Pregnancy Specific Beta 1 Glycoprotein (Sp-1) In Maternal Serum And Amniotic Fluid; Pre-Eclampsia, Small For Gestational Age Fetus And Fetal Distress., R. Silver, K. Heyborne, Kimberly Leslie
Pregnancy Specific Beta 1 Glycoprotein (Sp-1) In Maternal Serum And Amniotic Fluid; Pre-Eclampsia, Small For Gestational Age Fetus And Fetal Distress., R. Silver, K. Heyborne, Kimberly Leslie
Kimberly K. Leslie
Pregnancy specific beta 1 glycoprotein (SP-1) levels have been suggested to correlate with certain obstetrical complications. We compared maternal serum SP-1 levels in normal pregnancies (N = 82), and pregnancies complicated by pre-eclampsia (N = 37), small for gestational age fetuses (N = 8) and fetal distress (N = 13). We also compared levels of this protein in mid-trimester amniotic fluid samples obtained from normal (N = 47) and small for gestational age fetuses (N = 25). Despite trends towards low maternal serum and amniotic fluid SP-1 values in complicated pregnancies, there were no statistically significant differences in their levels …
Estrogen Receptors Are Identified In The Glioblastoma Cell Line U138mg., Kimberly Leslie, D. Keefe, S. Powell, F. Naftolin
Estrogen Receptors Are Identified In The Glioblastoma Cell Line U138mg., Kimberly Leslie, D. Keefe, S. Powell, F. Naftolin
Kimberly K. Leslie
OBJECTIVE: The antiestrogen tamoxifen has been found to be effective in decreasing glioblastoma cell proliferation, but the mechanism underlying this effect and whether it is through the estrogen receptor (ER) is controversial. The objective of this study was to determine whether ERs are present in three human glioblastoma cell lines--HS683, U138MG, and JHN J889H--using the most sensitive techniques available. METHODS: Ligand binding and flow cytometry were employed to identify estrogen and progesterone receptors. The reverse transcriptase-polymerase chain reaction was used to identify ER mRNA, and a novel reporter gene transfection assay demonstrated that the ER was capable of activating gene …