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Differentiation Of Renal Beta-Intercalated Cells To Alpha-Intercalated And Principal Cells In Culture., Geza Fejes-Tóth, Aniko Náray-Fejes-Tóth
Differentiation Of Renal Beta-Intercalated Cells To Alpha-Intercalated And Principal Cells In Culture., Geza Fejes-Tóth, Aniko Náray-Fejes-Tóth
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The renal collecting duct is a heterogenous epithelium consisting of intercalated cells (ICC) and principal cells (PC). The origin of this cellular heterogeneity is not clear. To test the hypothesis that the two cell types might originate from one another, pure populations of ICC (beta subtype) and PC were isolated by fluorescence-activated cell sorting and grown on permeable supports. After the monolayers reached confluence, the expression of ICC- and PC-specific functions and antigens was monitored. Cultures of sorted beta-ICC, in addition to expressing ICC-specific functions (such as an electrogenic H+ secretion) and antigens, progressively acquired PC functions (amiloride-sensitive Na+ transport …