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Full-Text Articles in Medical Biotechnology
Modelling Random Antibody Adsorption And Immunoassay Activity, Dana Mackey, Eilis Kelly, Robert Nooney
Modelling Random Antibody Adsorption And Immunoassay Activity, Dana Mackey, Eilis Kelly, Robert Nooney
Articles
One of the primary considerations in immunoassay design is optimizing the concentration of capture antibody in order to achieve maximal antigen binding and, subsequently, improved sensitivity and limit of detection. Many immunoassay technologies involve immobilization of the antibody to solid surfaces. Antibodies are large molecules in which the position and accessibility of the antigen-binding site depend on their orientation and packing density. In this paper we propose a simple mathematical model, based on the theory known as random sequential adsorption (RSA), in order to calculate how the concentration of correctly oriented antibodies (active site exposed for subsequent reactions) evolves during …
A Competitive Random Sequential Adsorption Model For Immunoassay Activity, Dana Mackey, Eilis Kelly, Robert Nooney
A Competitive Random Sequential Adsorption Model For Immunoassay Activity, Dana Mackey, Eilis Kelly, Robert Nooney
Conference papers
Immunoassays rely on highly specific reactions between antibodies and antigens and are used in biomedical diagnostics applications to detect biomarkers for a variety of diseases. Antibody immobilization to solid interfaces through random adsorption is a widely used technique but has the disadvantage of severely reducing the antigen binding activity and, consequently, the assay performance. This paper proposes a simple mathematical framework, based on the theory known as competitive random sequential adsorption (CRSA), for describing how the activity of immobilized antibodies depends on their orientation and packing density and generalizes a previous model by introducing the antibody aspect ratio as an …