Open Access. Powered by Scholars. Published by Universities.®
Amino Acids, Peptides, and Proteins Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Bacteriology (1)
- Bioinformatics (1)
- Biometry (1)
- Cancer Biology (1)
- Cell Biology (1)
-
- Cell and Developmental Biology (1)
- Diseases (1)
- Environmental Microbiology and Microbial Ecology (1)
- Environmental Public Health (1)
- Epidemiology (1)
- Genomics (1)
- Laboratory and Basic Science Research (1)
- Marine Biology (1)
- Medical Molecular Biology (1)
- Medical Sciences (1)
- Microbiology (1)
- Neoplasms (1)
- Organic Chemicals (1)
- Other Chemicals and Drugs (1)
- Physical Sciences and Mathematics (1)
- Public Health (1)
- Statistics and Probability (1)
- Keyword
-
- Bioinformatics (1)
- Biosynthetic gene cluster (1)
- Cancer (1)
- Chemotherapeutic drugs (1)
- Crystallization (1)
-
- Data mining (1)
- Epitopes (1)
- Gram-positive bacteria (1)
- Marine Streptomyces (1)
- Marine-derived sediment (1)
- Metagenomics (1)
- Nonribosomal peptide synthase (1)
- Polyketide (1)
- Protein crystallization; protein thermodynamics; crystallization mechanism; surface entropy; datamining; structural genomics (1)
- Proteins (1)
- Streptomyces (1)
- Thermodynamic stability (1)
- Publication Type
Articles 1 - 2 of 2
Full-Text Articles in Amino Acids, Peptides, and Proteins
Identification Of Novel Biosynthetic Gene Clusters Encoding For Polyketide/Nrps-Producing Chemotherapeutic Compounds From Marine-Derived Streptomyces Hygroscopicus From A Marine Sanctuary, Hannah Ruth Flaherty
Identification Of Novel Biosynthetic Gene Clusters Encoding For Polyketide/Nrps-Producing Chemotherapeutic Compounds From Marine-Derived Streptomyces Hygroscopicus From A Marine Sanctuary, Hannah Ruth Flaherty
Honors Theses and Capstones
Nearly one out of six deaths in 2020, around ten million people, were caused by cancer, making it a leading cause of death worldwide (WHO, 2022). This major public health issue, in addition to the rise of multidrug-resistant (MDR) pathogens, provides a high demand for the discovery of new pharmaceutical drugs to be used clinically to treat these conditions. The Streptomyces genus accounts to produce 39% of all microbial metabolites currently approved for human health, indicating its potential as an important species to study for antimicrobial and anticancer agents. The long linear genome of Streptomyces contains specialized sequences known as …
Understanding The Physical Properties That Control Protein Crystallization By Analysis Of Largescale Experimental Data, W. Nicholson Price Ii, Yang Chen, Samuel K. Handelman, Helen Neely, Philip Manor, Richard Karlin, Rajesh Nair, Jinfeng Liu, Michael Baran, John Everett, Saichiu N. Tong, Farhad Forouhar, Swarup S. Swaminathan, Thomas Acton, Rong Xiao, Joseph R. Luft, Angela Lauricella, George T. Detitta, Burkhard Rost, Gaetano T. Montelione, John T. Hunt
Understanding The Physical Properties That Control Protein Crystallization By Analysis Of Largescale Experimental Data, W. Nicholson Price Ii, Yang Chen, Samuel K. Handelman, Helen Neely, Philip Manor, Richard Karlin, Rajesh Nair, Jinfeng Liu, Michael Baran, John Everett, Saichiu N. Tong, Farhad Forouhar, Swarup S. Swaminathan, Thomas Acton, Rong Xiao, Joseph R. Luft, Angela Lauricella, George T. Detitta, Burkhard Rost, Gaetano T. Montelione, John T. Hunt
Law Faculty Scholarship
Crystallization is the most serious bottleneck in high-throughput protein-structure determination by diffraction methods. We have used data mining of the large-scale experimental results of the Northeast Structural Genomics Consortium and experimental folding studies to characterize the biophysical properties that control protein crystallization. This analysis leads to the conclusion that crystallization propensity depends primarily on the prevalence of well-ordered surface epitopes capable of mediating interprotein interactions and is not strongly influenced by overall thermodynamic stability. We identify specific sequence features that correlate with crystallization propensity and that can be used to estimate the crystallization probability of a given construct. Analyses of …