Other Analytical, Diagnostic and Therapeutic Techniques and Equipment Commons^™

Method Development And Validation Of The Quantitation Of 19 Antipsychotics Using Deuterated Internal Standards, Anthony S. Epps

Chemistry Theses

Antipsychotic drugs or neuroleptics are used primarily for psychiatric disorders such as schizophrenia, psychosis, and bipolar disorder. In forensic science antipsychotics are drugs of considerable interest because of their potential abuse, involvement in suicides, and they are frequently associated with sudden death investigations. Well-characterized and fully validated analytical data is necessary to generate reproducible and reliable results. As a result, data can be correctly interpreted and objectively demonstrate in its applicability for the intended use. This research has developed and validated a method that is selective, sensitive and accurate using liquid chromatography–tandem mass spectrometry (LC–MS/MS) for the simultaneous determination of …

Annexin A5-Conjugated Polymeric Micelles For Dual Spect And Optical Detection Of Apoptosis, Rui Zhang

Dissertations & Theses (Open Access)

Apoptosis, a form of programmed cell death, is critical to homoeostasis, normal development, and physiology. Dysregulation of apoptosis can lead to the accumulation of unwanted cells, such as occurs in cancer, and the removal of needed cells or disorders of normal tissues, such as heart, neurodegenerative, and autoimmune diseases. Noninvasive detection of apoptosis may play an important role in the evaluation of disease states and response to therapeutic intervention for a variety of diseases. It is desirable to have an imaging method to accurately detect and monitor this process in patients. In this study, we developed annexin A5-conjugated polymeric micellar …

Mcnamara 2011 Mpmicro - Multi-Probe Microscopy (10/31/2011), George Mcnamara

George McNamara

Multi-Probe Microscopy is an ~1500 page Word document summarizing what I know and/or found interesting in light microscopy, fluorescence microscopy and digital image analysis, from 1995-2005. Very little has been updated since 2005.

Immunologic Risk Prediction Model For Kidney Graft Function, Christina Diane Bishop

Doctoral Dissertations

Clinicians lack appropriate non-invasive methods to be able to predict, diagnose, and reduce the risk of rejection in the years following kidney transplantation. Protocol biopsies and monitoring of serum creatinine levels are the most common methods of monitoring graft function after transplant; however, they have several negative aspects. Use of traditional factors regarding donors and recipients such as Human Leukocyte Antigen (HLA) DNA typing, pre-transplant anti-HLA antibody levels, and basic demographics (age, ethnicity/race, gender), has proved inadequate for post-transplant graft monitoring past the first few years. We propose that by utilizing immunologic factors available to clinicians across the United States, …

The Development And Implementation Of An Anthropomorphic Head Phantom For The Assessment Of Proton Therapy Treatment Procedures, Paige A. Summers

Dissertations & Theses (Open Access)

Proton therapy has become an increasingly more common method of radiation therapy, with the dose sparing to distal tissue making it an appealing option, particularly for treatment of brain tumors. This study sought to develop a head phantom for the Radiological Physics Center (RPC), the first to be used for credentialing of institutions wishing to participate in clinical trials involving brain tumor treatment of proton therapy. It was hypothesized that a head phantom could be created for the evaluation of proton therapy treatment procedures (treatment simulation, planning, and delivery) to assure agreement between the measured dose and calculated dose within …

Evaluation Of Intensity Modulated Radiation Therapy (Imrt) Delivery Error Due To Imrt Treatment Plan Complexity And Improperly Matched Dosimetry Data, Jacqueline R. Tonigan

Dissertations & Theses (Open Access)

Intensity modulated radiation therapy (IMRT) is a technique that delivers a highly conformal dose distribution to a target volume while attempting to maximally spare the surrounding normal tissues. IMRT is a common treatment modality used for treating head and neck (H&N) cancers, and the presence of many critical structures in this region requires accurate treatment delivery. The Radiological Physics Center (RPC) acts as both a remote and on-site quality assurance agency that credentials institutions participating in clinical trials. To date, about 30% of all IMRT participants have failed the RPC’s remote audit using the IMRT H&N phantom. The purpose …

The Effect Of Shoulder Variation On Imrt And Smartarc For Head And Neck Cancer, Emily H. Neubauer

Dissertations & Theses (Open Access)

Purpose: First, to determine an average and maximum displacement of the shoulder relative to isocenter over the course of treatment. Second, to establish the dosimetric effect of shoulder displacements relative to correct isocenter alignment on the dose delivered to the target and the surrounding structures for head and neck cancer patients.

Method and Materials: The frequency of shoulder shifts of various magnitudes relative to isocenter was assessed for 4 patients using image registration software. The location of the center of the right and left humeral head relative to isocenter (usually C2) was found daily from CT on rails scans, and …

Initial Mlu Predicts The Relative Efficacy Of Two Grammatical Treatments In Preschoolers With Specific Language Impairments, Paul J. Yoder, Dennis L. Molfese, Elizabeth Gardner

Center for Brain, Biology, and Behavior: Faculty and Staff Publications

Purpose—We sought to confirm predictions based on past findings that pre-treatment mean length of utterance (MLU) would predict which of two grammatical treatments would best facilitate generalized and maintained grammatical development in preschoolers with specific language impairment (SLI).

Method—The participants were 57 preschoolers with specific language impairment (SLI). A randomized group experiment was used. The two grammatical treatments were Broad Target Recasts (BTR) and Milieu Language Teaching (MLT). MLU was assessed at Time 1 in two conversational language samples. Growth rate of productive grammar was quantified using growth curve modeling on the Index of Productive Syntax (IPSyn) from …

Clinical Impact Of Couch Top And Rails On Imrt And Arc Therapy, Kiley B. Pulliam

Dissertations & Theses (Open Access)

Purpose: To evaluate the clinical impact of the Varian Exact Couch on dose and volume coverage to targets and critical structures and tumor control probability (TCP) for 6-MV IMRT and Arc Therapy.

Methods: Five clinical prostate patients were planned with both, 6-MV 8-field IMRT and 6-MV 2-field RapidArc using the Eclipse treatment planning system (TPS). These plans neglected treatment couch attenuation, as is standard clinical practice. Dose distributions were then recalculated in Eclipse with the inclusion of the Varian Exact Couch (imaging couch top) and the rails in varying configurations. The changes in dose and coverage were evaluated using the …

Interictal Magnetoencephalographic Findings Related With Surgical Outcomes In Lesional And Nonlesional Neocortical Epilepsy, Rui Zhang, Ting Wu, Yingying Wang, Hongyi Liu, Yuanjie Zou, Wen Liu, Jing Xiang, Chaoyong Xiao, Lu Yang, Zhen Fu

Center for Brain, Biology, and Behavior: Faculty and Staff Publications

Purpose: To investigate whether interictal magnetoencephalography (MEG) concordant with other techniques can predict surgical outcome in patients with lesional and nonlesional refractory neocortical epilepsy (NE).

Methods: 23 Patients with lesional NE and 20 patients with nonlesional NE were studied. MEG was recorded for all patients with a 275 channel whole-head system. Synthetic aperture magnetometry (SAM) with excess kurtosis (g2) and conventional Equivalent Current Dipole (ECD) were used for MEG data analysis. 27 Patients underwent long-term extraoperative intracranial video electroencephalography (iVEEG) monitoring. Surgical outcomes were assessed based on more than 1-year of post-surgical follow-up using Engel classification system.

Results: As we …

Determining A Method For Rendering Low Cost Cdse(Zns) Core(Shell) Quantum Dots Aqueous Soluble Via Amphiphilic Polymer Wrapping, Patrick Mcbride

Materials Engineering

Herein is described the procedure of two amphiphilic polymer wrapping techniques that may be employed for obtaining aqueous soluble quantum dots (QDs) for use in biological fluorescent imaging applications. The advent of QDs has led to new nanoscale fluorescent materials that exhibit unparalleled quantum yields (QYs), high resistance to photobleaching, tunable emissions, and
absorption over a large optical range. However, the QD synthesis employed here at Cal Poly to obtain bright, photostable CdSe(ZnS) core(shell) QDs involves the use of organic solvents and surfactants, leading to hydrophobic QDs. Since all of biology relies on aqueous solubility, this hydrophobicity creates a major …

Why Is There Still So Much Confusion About Vo2 Plateau? A Re-Examination Of The Work Of A.V. Hill, Richard Vincent Castle

Masters Theses

Maximal oxygen uptake (VO_2max) is regarded as the gold standard for assessing aerobic fitness. In 1923, Hill et al. proposed that VO_2max represents the maximal ability of the body to take in and consume O₂ during strenuous exercise. Recently, however, controversy has arisen over the issue of whether a leveling off, or "plateau" in VO₂ is necessary to verify attainment of VO_2max. Purpose: To compare two different VO_2max protocols and determine if both protocols show direct evidence of an upper limit on VO₂. Methods: Nine runners (18-35 years old) completed …

Development Of An Autonomous Mammalian Lux Reporter System, Daniel Michael Close

Doctoral Dissertations

Since its characterization, the definitive shortcoming of the bacterial luciferase (lux) bioluminescent reporter system has been its inability to express at a functional level in the eukaryotic cellular background. While recent developments have allowed for lux function in the lower eukaryote Saccharomyces cerevisiae, they have not provided for autonomous function in higher eukaryotes capable of serving as human biomedical proxies. Here it is reported for the first time that, through a process of poly-bicistronic expression of human codon-optimized lux genes, it is possible to autonomously produce a bioluminescent signal directly from mammalian cells. The low background of …

The Acute Effects Of Static Stretching Of The Gastrocnemius On Limits Of Stability In Young Adults Versus Elderly Adults, Matt Bugnet, Kirk Player

UNLV Theses, Dissertations, Professional Papers, and Capstones

Background and Purpose: Balance is an important part of everyday life for all individuals with many body systems interacting to achieve optimal balance. Proprioceptive organs aid in this interaction, while also acting as stretch receptors. Therefore, it is possible that stretching may influence the overall balance of the individual. The purpose of this study was to investigate the acute effects of stretching on dynamic balance of healthy young and elderly adults. The two groups were used to determine if stretching may discriminately affect balance at different ages.

Subjects: Thirty healthy adults between the ages of 18 and 35 …

Synthesis And Analytical Evaluation Of Folate Conjugates For Use In Cancer Cell Detection, Pradipkumar Koshiya

All Capstone Projects

Folate-targeted dyes have been used for the detection of tumor tissue and isolated cancer cells in both the animal tumor models as well as in human cancer patients. Folate receptors (FR) are abundantly present on several cancerous cell types but they are found less in normal human cells. There are several imaging agents that can be linked with folic acid and used for the detection of tumor. These imaging agents could be protein toxins, chemotherapeutic agents, gene therapy vectors, oligonucleotides, radioimaging agents, magnetic resonance imaging contrast agents and liposomes with entrapped drugs, radiotherapeutic agents and/or immunotherapeutic agents. This research showed …

Detection Of Protein By Microdrop Analysis, Yogeshkumar Radadiya

All Capstone Projects

Analysis of protein depends particularly on protein concentration. Protein concentration measurement is the most important part in the research work to conduct protein-related studies. Although there are many methods available for this purpose, each method has certain limitations. The aim of the experiment is to develop either new or modified analytical method for the analysis and detection of protein using newly introduced micro-plate reader equipped with Take-3 microplate with the help of Gen-5 software. An ideal assay should be simple and easy to carry out. Another aspect to be taken into consideration are low inference, stability of measured components and …

Detection Of Folate Receptor From Fr+ Cancer Cells, Darpan Patel

All Capstone Projects

The aim was to detect cancer cells by using folate–PEG-Biotin probe with fluorescently labeled streptavidin for targeted drug delivery of anti-cancer drugs and diagnose cancer cells. Folate-PEG-Biotin (synthesized by Dr. Henne and Mr.Rohan Patel) was purified by HighPeformanceLiquidChromatography (HPLC) analysis and with the help of Liquid Chromatography/Mass Spectrometry (LC/MS) its identity was confirmed. Folate is a basic composition of cell metabolism in both synthesis of DNA and proteins. Growing cancer cells require high level of folic acid. Folate Receptor-α (FR-α) is a membrane bound protein having high affinity for folic acid and serves to transport of folate into cells. Poly …

Neuromagnetic Measures Of Word Processing In Bilinguals And Monolinguals, Yingying Wang, Jing Xiang, Jennifer Vannest, Tom Holroyd, Daria Narmoneva, Paul Horn, Yinhong Liu, Douglas Rose, Ton Degrauw, Scott Holland

Center for Brain, Biology, and Behavior: Faculty and Staff Publications

Objective: This study aimed to use magnetoencephalography (MEG) to examine the question of whether Mandarin-English bilingual speakers recruit the same cortical areas or develop distinct language-specific networks without overlaps for word processing.

Methods: Eight healthy Mandarin-English bilingual adults and eight healthy English monolingual adults were scanned while single-word paradigms were audio-visually presented.

Results: Our results showed significantly stronger beta-band power suppression in the right inferior parietal lobe (IPL) covering the supramarginal gyrus (BA 40) and angular gyrus (BA 39) for bilinguals when processing Mandarin versus English. Moreover, there were no significant differences between bilinguals and monolinguals in the left inferior …

Sleep Assessments In Healthy School-Aged Children Using Actigraphy: Concordance With Polysomnography, Karen Spruyt, David Gozal, Ehab Dayyat, Adrienne Roman, Dennis L. Molfese

Center for Brain, Biology, and Behavior: Faculty and Staff Publications

Actigraphic recordings (ACT) are widely used in school children as a less intrusive and more extended approach to evaluation of sleep problems. However, critical assessment of the validity and reliability of ACT against overnight polysomnography (NPSG) are unavailable. Thus, we explored the degree of concordance between NPSG and ACT in school-aged children to delineate potential ACT boundaries when interpreting pediatric sleep. Non-dominant wrist ACT was simultaneously recorded with NPSG in 149 healthy school-aged children (4.1 to 8.8 years old, 41.7% boys and 80.4% Caucasian) recruited from the community. Analyses were limited to the Actiware (MiniMitter-64) calculated parameters originating from 1-min …

Sleep Duration, Sleep Regularity, Body Weight, And Metabolic Homeostasis In School-Aged Children, Karen Spruyt, Dennis L. Molfese, David Gozal

Center for Brain, Biology, and Behavior: Faculty and Staff Publications

OBJECTIVE: The goal was to explore the effects of duration and regularity of sleep schedules on BMI and the impact on metabolic regulation in children.

METHODS: Sleep patterns of 308 community-recruited children 4 to 10 years of age were assessed with wrist actigraphs for 1 week in a cross-sectional study, along with BMI assessment. Fasting morning plasma levels of glucose, insulin, lipids, and high-sensitivity C-reactive protein also were measured for a subsample.

RESULTS: Children slept 8 hours per night, on average, regardless of their weight categorization. A nonlinear trend between sleep and weight emerged. For obese children, …

Seguimiento A Los Graduados Entre Enero Del 2000 Y Diciembre Del 2010 Del Programa De Optometría De La Universidad De La Salle, David Andrés Torres Dávila, Juli Marcela Cárdenas Flórez, Nancy Piedad Molina Montoya

Ciencia y Tecnología para la Salud Visual y Ocular

La Universidad de La Salle hace seguimiento a sus graduados para mejorar la calidad de la educacióny sus procesos de autoevaluación y redimensión curricular. Objetivo hacer seguimientoa los graduados en el periodo 2000-2010 del programa de Optometría. Materiales y métodosse encuestó a los egresados sobre: actualización de datos, formación académica, desempeño yubicación laboral, actividades científico-académicas y satisfacción. Se tabuló e integró la informacióna la base de datos existente y se realizó análisis estadístico de los resultados. Resultados se encuestaron 182 egresados en el periodo establecido (28,9%): el 1,6% ha cursado maestría, el12,6% especializaciones, el 33,5% diplomados y el 13,7% …

Influencia Del Sistema Visual En El Aprendizaje Del Proceso De Lectura, Sandra Milena Medrano Muñoz

Ciencia y Tecnología para la Salud Visual y Ocular

Cada vez es más frecuente la remisión a consulta optométrica de niños con signos de problemas deaprendizaje de la lectura evidenciados por profesores o en ocasiones directamente por los padresde familia. Sin embargo, muchas veces el optómetra carece de las herramientas necesarias paramanejar estos casos; quizás la dificultad se encuentre en dos dudas: ¿cómo puede influir el sistemavisual en los procesos de lectura?, y ¿cómo desde la optometría se puede contribuir a un diagnósticoy al manejo de problemas de aprendizaje de la lectura? En la presente revisión se intentarádar respuesta a estas preguntas partiendo de la premisa de que el …

Valoración Optométrica Y Socioeconómica De Personas Asociadas A Corpocima De La Localidad De Bosa, Sandra Medrano, Marcela Camacho, María Inés Baquero, Marleny Cardona

Ciencia y Tecnología para la Salud Visual y Ocular

Introducción este proyecto de investigación se inició en el 2008 y culminó en el 2010. Fue realizadopor un grupo interdisciplinario conformado por profesionales del área de economía, trabajosocial, psicología y optometría. Objetivo potenciar capacidades y empoderamiento de hombres ymujeres con discapacidad visual con miras al fortalecimiento de la gobernabilidad democrática, laincidencia en las políticas públicas, consecución del desarrollo humano y desarrollo de circuitoseconómicos en la localidad de Bosa. Desde el punto de vista de la optometría, el objetivo específicofue generar alternativas desde la perspectiva del desarrollo humano sustentable, mediantela valoración del estado visual y el diagnóstico de personas con discapacidad …

Turismo En Salud: Una Tendencia Mundial Que Se Abre Paso En Colombia, Adriana Milena Barriga Castro, Martha Lucía Farías Villarraga, Ángela Liliana Ruiz Barreto, Angie Jennefer Sánchez Victoria, Wilson Giovanni Jiménez Barbosa

Ciencia y Tecnología para la Salud Visual y Ocular

El concepto de turismo en salud no es nuevo; básicamente se trata de personas que viajan fuera desus países de residencia con el objetivo de buscar servicios médicos o de bienestar en otros países.Los factores que han conducido a la creciente popularidad de esta modalidad de turismo son elalto costo de la atención en salud en el país de origen, largos tiempos de espera para ciertos procedimientos,la facilidad y accesibilidad de los viajes internacionales y las mejoras en la tecnología ylos estándares de atención en muchos países. Los estándares de calidad son importantes cuandose trata del cuidado de la salud, …

Determinación De Los Cambios En La Función De Sensibilidad Al Contraste Posterior A La Terapia Visual En Pacientes Con Diagnóstico De Ambliopía Refractiva, Sandra Milena Medrano Muñoz, Alejandro León Álvarez, María Jesús Izquierdo

Ciencia y Tecnología para la Salud Visual y Ocular

Introducción se ha descrito que la sensibilidad al contraste se ve afectada en ojos ambliopes y que mejora luego de la terapia visual. Objetivo determinar los cambios en la función visual desensibilidad al contraste posterior a terapia en niños con diagnóstico de ambliopía refractiva. Materialesy métodos se realizó un estudio retrospectivo en una muestra de veintiún pacientes conedades comprendidas entre los cinco y quince años del Centro de Especialización Optométrica(CEO) en Pamplona, España. Se incluyeron historias clínicas con diagnóstico de ambliopía refractivaque asistieron al CEO durante el 2007 al 2009. Resultados la agudeza visual presentócambios estadísticamente significativos en visión lejana …

La Baja Visión En Colombia Y En El Mundo, Yenifer Zuley Cañón Cárdenas

Ciencia y Tecnología para la Salud Visual y Ocular

La visión es uno de los sentidos más significativos del hombre, pues es la ventana al universoexterior. El individuo en esencia se considera un ser visual, es por eso que toda persona posee elderecho a la visión. En Colombia son pocas las investigaciones en baja visión (definida comouna reducción importante de la función visual que no puede ser totalmente corregida por gafas,lentes de contacto, tratamiento médico o quirúrgico, y generalmente interfiere con actividadescomo cocinar, leer y escribir, conducir un auto o caminar), siendo esta una de las razones por lascuales hay desconocimiento de su prevalencia, incidencia, principales causas, el manejo …

Concordancia Entre Dos Técnicas Subjetivas Para Determinar La Refracción En Adultos Jóvenes, Alejandro León Álvarez, Jorge Mario Estrada Álvarez, Johana Marcela Giraldo Ruiz, Luis Alejandro Giraldo Sánchez

Ciencia y Tecnología para la Salud Visual y Ocular

La refracción subjetiva es una técnica que compara un lente con otro para llegar a una combinaciónde lentes que resulte en el máximo de agudeza visual. Estudios han evaluado la concordanciaintra/inter observadora y con otras técnicas objetivas o subjetivas, principalmente para el cilindrocruzado de Jackson (JCC), pero pocas para el emborronamiento más dial astigmático (EmD).Objetivo determinar la reproducibilidad de los métodos subjetivos EmD y JCC y la concordanciade estos con el procedimiento objetivo de retinoscopia estática. Método se realizó un estudiodescriptivo de concordancia con 190 sujetos, con medición independiente para cada técnica pordos evaluadores. En el análisis estadístico de …

Evaluación De Las Aberraciones De Alto Orden Corneales Y Oculares Mediante El Índice Root Mean Square (Rms) Con Dos Lentes De Contacto Blandos Asféricos En Pacientes Con Astigmatismo Miópico Bajo, Natali Gutiérrez Rodríguea, Elisabeth Suárez Pisciotti, Sergio Mario García

Ciencia y Tecnología para la Salud Visual y Ocular

Objetivo determinar cuál lente de contacto blando asférico (Pure Vision o Definition AC) modificamás el valor Root Mean Square (RMS ) de alto orden en las aberraciones oculares y cornealesen pacientes con astigmatismo miópico bajo. Materiales y métodos se estudiaron cuarenta ojoscon astigmatismo miópico con la regla con esferas no mayores a -3,00 D y cilindros menores a-1,25 D, no usuarios de lentes de contacto a quienes se les realizaron aberrometrías con el aberrómetroCoas y con el topógrafo Keratron Scout en tres momentos: sin lente de contacto, conlente Pure Vision y con Definition AC. Resultados con ambos lentes se aumenta …

Errores Refractivos En Niños De Tres A Siete Años En La Localidad De Chapinero De La Ciudad De Bogotá, Luisa Fernanda Figueroa Olarte, Nancy Piedad Molina Montoya

Ciencia y Tecnología para la Salud Visual y Ocular

La medición de los errores refractivos desde temprana edad permite tomar la decisión sobre si seprescribe o no corrección óptica teniendo en cuenta el desarrollo, el error refractivo y la edad. Loscriterios de corrección están basados en valores de referencia; en Colombia se tienen en cuentalos parámetros establecidos por escuelas europeas y norteamericanas derivados de estudios poblacionales,debido a la falta de investigaciones sobre valores normales. El presente artículo presentaresultados parciales de una investigación realizada por docentes del programa de Optometría de laUniversidad de La Salle. Objetivo determinar valores de referencia de error refractivo en niños deentre tres y siete años …

Entendiendo E Interpretando Las Aberraciones Ópticas, Rosario Vidal Olarte

Ciencia y Tecnología para la Salud Visual y Ocular

Los avances tecnológicos recientes permiten evaluar de manera objetiva y subjetiva la calidad visual. es por ello que el optómetra como profesional de la salud visual y ocular, en la aplicación y la práctica clínica, debe tener en cuenta el papel tan importante que desempeña al prescribir una corrección óptica, según la cantidad y calidad en la imagen visual, puesto que determina un factor cuantitativo, como es la agudeza visual desde el punto de vista subjetivo del paciente, y otro cualitativo, como sería la calidad y nitidez con que se pueden ver las imágenes. La óptica física aplicada a la …