Other Analytical, Diagnostic and Therapeutic Techniques and Equipment Commons^™

Synthesis And Analytical Evaluation Of Folate Conjugates For Use In Cancer Cell Detection, Pradipkumar Koshiya

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Folate-targeted dyes have been used for the detection of tumor tissue and isolated cancer cells in both the animal tumor models as well as in human cancer patients. Folate receptors (FR) are abundantly present on several cancerous cell types but they are found less in normal human cells. There are several imaging agents that can be linked with folic acid and used for the detection of tumor. These imaging agents could be protein toxins, chemotherapeutic agents, gene therapy vectors, oligonucleotides, radioimaging agents, magnetic resonance imaging contrast agents and liposomes with entrapped drugs, radiotherapeutic agents and/or immunotherapeutic agents. This research showed …

Detection Of Protein By Microdrop Analysis, Yogeshkumar Radadiya

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Analysis of protein depends particularly on protein concentration. Protein concentration measurement is the most important part in the research work to conduct protein-related studies. Although there are many methods available for this purpose, each method has certain limitations. The aim of the experiment is to develop either new or modified analytical method for the analysis and detection of protein using newly introduced micro-plate reader equipped with Take-3 microplate with the help of Gen-5 software. An ideal assay should be simple and easy to carry out. Another aspect to be taken into consideration are low inference, stability of measured components and …

Detection Of Folate Receptor From Fr+ Cancer Cells, Darpan Patel

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The aim was to detect cancer cells by using folate–PEG-Biotin probe with fluorescently labeled streptavidin for targeted drug delivery of anti-cancer drugs and diagnose cancer cells. Folate-PEG-Biotin (synthesized by Dr. Henne and Mr.Rohan Patel) was purified by HighPeformanceLiquidChromatography (HPLC) analysis and with the help of Liquid Chromatography/Mass Spectrometry (LC/MS) its identity was confirmed. Folate is a basic composition of cell metabolism in both synthesis of DNA and proteins. Growing cancer cells require high level of folic acid. Folate Receptor-α (FR-α) is a membrane bound protein having high affinity for folic acid and serves to transport of folate into cells. Poly …

Ruthenium Complexes As Dna Photonucleases In Treatment Of Malignant Skin Carcinoma, Devi Kalyan Karumanchi

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A variety of Ruthenium complexes possessing terpyridine complexes as ligands in their coordination sphere have been investigated extensively as DNA photo nucleases in vitro. This work has led to the realization that compounds of Ruthenium bind to DNA purines and that the interactions are of an unprecedented bridging variety. Owing to the molar absorptivity of these complexes in the visible region, direct cleavage of duplex DNA by these complexes requires irradiation in the range of 400-500 nm.

Although the observation of DNA photo cleavage in vitro makes these systems good candidates for further investigation, it does not provide any information …