Medicine and Health Sciences Commons^™

Cryo-Em Structures Of Eastern Equine Encephalitis Virus Reveal Mechanisms Of Virus Disassembly And Antibody Neutralization, Saif Hasan, Chengqun Sun, Arthur S. Kim, Yasunori Watanabe, Chun-Liang Chen, Thomas Klose, Geeta Buda, Max Crispin, Michael S. Diamond, William B. Klimstra, Michael G. Rossmann

Department of Biological Sciences Faculty Publications

Alphaviruses are enveloped pathogens that cause arthritis and encephalitis. Here, we report a 4.4-Å cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans. Our analysis provides insights into viral entry into host cells. The envelope protein E2 showed a binding site for the cellular attachment factor heparan sulfate. The presence of a cryptic E2 glycan suggests how EEEV escapes surveillance by lectin-expressing myeloid lineage cells, which are sentinels of the immune system. A mechanism for nucleocapsid core release and disassembly upon viral entry was inferred based on pH changes and capsid …

A Comparison Of Student Pharmacist Clinical Interventions During An Internal Medicine Advanced Pharmacy Practice Experience (Appe) In A High-Income Versus Low-Income Setting, Ellen Schellhase, Monica Miller, Shelby Wilkinson, Rakhi Karwa

Engagement & Service-Learning Summit

Objectives: Compare and contrast the number and type of interventions made by student pharmacists who participated in an adult internal medicine APPE in the US to an internal medicine APPE in Kenya.

Methods: A US-based faculty-led, four-week internal medicine APPE with interprofessional team rounding that provides care for underserved patients in an urban setting and hosts 16 students annually was compared to a faculty-led, eight-week internal medicine APPE in Kenya that hosts on average 24 students annually. Starting May 2017 – January 2018 a standard clinical intervention collection form was used by all students on these two APPEs starting …

Assessment Of Intercultural Learning Within An Interdisciplinary Empathy Course Within An Honors College, Ellen Schellhase, Monica Miller

Engagement & Service-Learning Summit

Objective: The objective was to assess the intercultural learning of students enrolled in an interdisciplinary, active learning course with a focus on empathy and healthcare within an Honors College.

Methods:

This 2-credit course met twice weekly for active-learning session for 16 weeks. Topics covered included cultural dimensions, mindfulness, learning styles and four intercultural core competencies. Assignments included an intercultural development plan, on-line discussion board reflections for three required service-learning activities and completion of a Cultural Competence Badge. Students assessed their empathy using several different scales and completed the Intercultural Development Inventory (IDI) assessment at the beginning and end of the …

Construction And Nursing Students, Building A Playground For Bauer Family Services, Daphene Koch, Mary Erin Hoying, Deborah A. Spoerner, Rebecca C. Johnson, Abby Berg

Engagement & Service-Learning Summit

Poster Session

Animal-Assisted Interventions For Children With Autism Spectrum Disorders: A Survey Of French Facilities, Claire Philippe-Peyroutet, Marine Grandgeorge

People and Animals: The International Journal of Research and Practice

Our survey of animal-assisted interventions (AAI) offered by French intervention facilities aimed to examine and describe the range of AAI for autism spectrum disorder (ASD) children. We invited 2,302 facilities to reply to an online questionnaire. The responses to our survey (n = 386) revealed that animals were used extensively as a complementary intervention. Most AAI sessions were in groups. Various animal species (especially horses and dogs) and facility staff members as well as external collaborators were involved. Numerous benefits (e.g., enhanced well-being, self-esteem, and socialization) were reported. Facilities face difficulties in connection with AAI including finances, staffing, and …

Assessment Of Recent Cases Of Animal Hoarding In Germany: The Challenge For Animal Shelters And Public Authorities, Sophie Arnold, Henriette Mackensen, Evelyn Ofensberger, Brigitte Rusche

People and Animals: The International Journal of Research and Practice

Animal hoarding is a severe problem in the field of human-animal interaction. The goal of this study was to assess the current situation of animal hoarding in Germany. Reports of animal hoarding cases were collected from animal shelters and public media between January 2012 and December 2015; 120 cases were analyzed. A total of 9,174 animals were hoarded during the investigated time period. The results showed that cases involving cats were most common, followed by cases involving dogs and small mammals. The average number (x¯) of animals hoarded per case was 76 (x˜ = 43). Small mammals were hoarded in …

Nurse Practitioner Students: Providing Wellness Screenings To Underserved Older Adults, Rexanna Tatlock

Purdue Journal of Service-Learning and International Engagement

Rexanna Tatlock (BSN, RN, CCRN) received a BSN from Saint Francis College in 2003. As a registered nurse, she specialized in critical care nursing and eventually enrolled in the Adult Gerontology Nurse Practitioner master’s program in Purdue University’s School of Nursing in 2016. Upon graduation in May 2018, Rexanna has planned to focus on primary health care. The service-learning project provided primary care clinical experience, while meeting the needs of an underserved older adult population. This article will discuss the community partnership, a needs assessment, and a wellness screening offered to Fairington Apartments (FA) residents.

My Experience In Swaziland With Give Hope, Fight Poverty, Megan Kaser

Purdue Journal of Service-Learning and International Engagement

Megan Kaser, a recent 2017 alum in the College of Health and Human Sciences at Purdue University, describes her experience with Give Hope, Fight Poverty (GHFP)—a nonprofit organization in Indianapolis, Indiana. She is currently pursuing a master’s degree in physician assistant studies. GHFP’s mission is “to foster philanthropy domestically by designing service-learning programs that engage U.S. college students with rural communities in Swaziland, Africa, and work together to educate, empower, and lift orphaned and vulnerable children—particularly those living in child-headed households— out of poverty” (Give Hope, Fight Poverty, n.d.). By incorporating college students in the implementation of GHFP orphan education …

The Pharmaceutical Industry: A Pharmacy Student's Guide, Christian Egly, Yaman Kaakeh

Purdue Journal of Service-Learning and International Engagement

Christian Egly is a fourth-year (P4) pharmacy student in the Purdue University College of Pharmacy. During his years at Purdue, he worked in labs performing bench research in clinical pharmacology and biochemistry. He plans to work in the pharmaceutical industry after graduation. During his fourth year, he completed rotations in business development at Kashiv Pharma, LLC, and was hired there for an internship in 2017. In the article, Christian describes his personal experiences at Kashiv Pharma, LLC, and how industry can positively affect patient communities.

Peer Health Educators On Campus: Teaching Safe Medication Practices, Baylee Q. Bryan, Olga O. Vlashyn, Stephanie A. Deremiah, Murphy K. O'Toole, Katherine M. Joseph, Jane E. Krause

Purdue Journal of Service-Learning and International Engagement

Baylee Q. Bryan, Olga O. Vlashyn, Stephanie A. Deremiah, and Murphy K. O’Toole are advanced professional students in the Doctor of Pharmacy program at Purdue University, graduating in May 2019. Katherine M. Joseph is a senior in the School of Nursing, graduating in December 2018. Jane E. Krause (Clinical Associate Professor of Pharmacy Practice, Purdue College of Pharmacy) served as a faculty mentor for this project. In this article, the authors describe their service-learning project intended to increase awareness on campus regarding prescription drug abuse and misuse.

Smart Disease Prevention App: Informing The Public In Their Own Geographic Location, Apoorva Sulakhe, Shafali Rana, Zoe Disori, William Nogay, Kyle Plummer, Meredith Shannon, Morgan Young, Alyssa Zielinski, Vincent G. Duffy

Purdue Journal of Service-Learning and International Engagement

Apoorva Sulakhe and Shefali Rana are graduate students in the School of Industrial Engineering at Purdue. They have both been teaching assistants under their coauthor, Dr. Vincent Duffy, while supervising multiple projects. Coauthors Zoe Disori, William Nogay, Kyle Plummer, Meredith Shannon, Morgan Young, and Alyssa Zielinski are listed in alphabetical order. They were all seniors in School of Industrial Engineering at the time of this project in 2017. The purpose of their study, described in this article, was to develop an application to provide users with accurate information about diseases spreading in their geographic locations.

Leveraging Technology: Enhancing Study Time Through Competition, Ming Yang, Lindsey Eble, Patricia Darbishire

Purdue Journal of Service-Learning and International Engagement

Ming (Max) Yang and Lindsey Eble are students in their final year of the Doctor of Pharmacy program in the Purdue University College of Pharmacy. Max is originally from Chesterfield, Missouri, and Lindsey is from Newburgh, Indiana. After graduation in May 2018, Max will complete residency training at Aurora St. Luke’s Medical Center in Milwaukee, Wisconsin. Lindsey will complete residency training at Barnes-Jewish Hospital in St. Louis, Missouri. Both plan to work in a hospital or clinic setting providing patient care within an interdisciplinary healthcare team. They both worked under the guidance of Dr. Patricia Darbishire to develop a website …

"Wrapping" Up In Kenya: A Student’S Learning Experience In Eldoret, Kenya, Alexander R. Mills, Monica L. Miller, Rakhi Karwa, Sonak D. Pastakia, Aileen Y. Chang, Edith Tonui, Sara Fletcher, Phelix Were, Ellen Schellhase

Purdue Journal of Service-Learning and International Engagement

Alexander R. Mills received his Doctor of Pharmacy degree in May 2017 and has completed a PGY-1 community-based pharmacy practice resident with Walgreen Company and Purdue University College of Pharmacy. During his time at Purdue, he was heavily involved with Purdue’s chapter of The American Red Cross Club, serving as the president and coordinator of community outreach programs. Upon completing his PGY-1, he will continue to pursue his aspirations to care for the medically underserved while currently completing a PGY-2 residency specializing in ambulatory care and academia at the University of Mississippi in Jackson.

Indiana University Health: A Student, An Institution, And The People, Allison Bates

Purdue Journal of Service-Learning and International Engagement

Allison Bates is a student in Purdue University’s Doctor of Pharmacy program with an anticipated graduation date of May 2019. Her future career aspirations are to practice in ambulatory care pharmacy or in an oncology setting. She has volunteered with the Joseph Maley Foundation since 2009 and with the “It’s My Closet” organization in Lafayette since 2014. This article describes her experience working as an intern with Dr. Dawn Moore, Vice President of Pharmacy and Chief Pharmacy Officer at IU Health.

Value-Added Services: Incorporating Pharmacy Students In A Hospital Setting, Megan Unger

Purdue Journal of Service-Learning and International Engagement

Megan Unger, a PharmD candidate in her fourth professional year in the College of Pharmacy, completed an introductory pharmacy practice experience at Deaconess Hospital in Evansville, Indiana, during her third professional year. This was the third of thirteen experiential learning rotations that she will complete prior to graduation in 2019. Her future plans include pursuing a postgraduate residency training program. In this article, Megan highlights the value-added services that pharmacy students can provide to institutional pharmacy settings and the patients served.

Shanghai, China: “Nontraditional” Traditional Chinese Medicine, Joshua Martin

Purdue Journal of Service-Learning and International Engagement

Joshua Martin is a second-year PharmD student in the Purdue University College of Pharmacy. Within the College of Pharmacy, he is the president-elect of the Industry Pharmacist Organization (IPhO), is on the Orientation Steering Committee for newly admitted PharmD students, and preforms nanoparticle research within the Industrial and Physical Pharmacy sector of the College. He hopes to pursue a career in the pharmaceutical industry where he can positively impact patients on a global scale. Josh comes from a family of Boilermakers and avidly supports the Black and Gold. In this article, he describes his experience studying at Shanghai University of …

Food For Thought: My Experience With The Food Finders “Head’S Up” Program And Community Outreach, Amanda Hornbach

Purdue Journal of Service-Learning and International Engagement

Amanda Hornbach is a medical student at the Indiana University School of Medicine’s West Lafayette (IUSM–WL) campus. During the summer between her first and second years of medical school, Hornbach developed a one-time, interactive educational curriculum for children participating in a local summer-long lunch and educational series through the Food Finders Food Bank. This project was completed as part of the IUSM–West Lafayette Primary Care Scholars summer program. This article outlines her experiences, her development of session material, and what she learned as a result of completing the program. Development of this curriculum dovetails her interests in pediatrics and health …

Fatal General Aviation Accidents In Furtherance Of Business (1996–2015): Rates, Risk Factors, And Accident Causes, Scott Burgess, Stewart Boyd, Douglas Boyd

Journal of Aviation Technology and Engineering

Introduction: General aviation missions related to furtherance of business potentially carry higher risk (operations in adverse weather, at night, for longer distances) than those undertaken for recreation. The study herein was undertaken to determine fatal accident rates, proportions, and risk factors/causes.

Method: The National Transportation Safety Board aviation accident database was queried for accidents (1996–2015). Annual fleet times were from the general aviation annual activity survey. Statistical analyses used Poisson distributions, proportion testing, and Cohen’s Kappa tests.

Results: The fatal accident rate for business operations was three- to six-fold lower than the rate for recreational flights with a decline evident …

Reimagining Print Materials In A Health Science Context: Creating And Marketing A Wellness Collection, Margaret Ansell, Ariel Pomputius

Charleston Library Conference

In the healthcare field in which out-of-date information can harm patients, the currency and immediacy of digital collections is highly valued. As a result, many health science libraries have deselected much of their print collections (Haapanen, Kultamaa, Ovaska, & Salmi, 2015; Lingle & Robinson, 2009; Tobia & Hunnicutt, 2008; Xiaoli & Kopper, 2005). However, print materials continue to be valued by health science library users for a variety of purposes (Houghton, 2017; Watson, 2016). This paper describes how one academic health science center library found a role for print materials in the context of a wellness initiative, which (1) gave …

Where Are We? Providing Information For The Clinical Enterprise (17th Health Sciences Lively Lunch), Ramune K. Kubilius, Jean Gudenas, Laura Schimming, Jonathan Shank, Vida Vaughn, Neal Nixon

Charleston Library Conference

If the past is prologue to the future, where are we today? Though they are diverse, most academic health sciences libraries have historically described and continue to outline their missions as being directed to serving the education, research, and clinical information needs of their user populations. Over the years, Charleston Conference Health Sciences Lively Lunches have covered many themes, and in 2012, the focus was on the point of care information tools landscape. The 2017 17th Health Sciences Lively Lunch focused on issues and challenges of providing clinicians and clinical affiliates with access not only to point of care tools, …

De Novo Sequence And Copy Number Variants Are Strongly Associated With Tourette Disorder And Implicate Cell Polarity In Pathogenesis, Sheng Wang, Jeffrey D. Mandell, Yogesh Kumar, Nawei Sun, Montana T. Morris, Juan Arbelaez, Cara Nasello, Shan Dong, Clif Duhn, Xin Zhao, Zhiyu Yang, Shanmukha S. Padmanabhuni, Dongmei Yu, Robert A. King, Andrea Dietrich, Najah Khalifa, Niklas Dahl, Alden Y. Huang, Benjamin M. Neale, Giovanni Coppola, Carol A. Mathews, Jeremiah M. Scharf, Tourette International Collaborative Genetics Study (Tic Genetics), Tourette Syndrome Genetics Southern And Eastern Europe Initiative (Tsgenesee), Tourette Association Of America International Consortium For Genetics (Taaicg), Thomas V. Fernandez, Joseph D. Buxbaum, Silvia De Rubeus, Dorothy E. Grice, Jinchuan Xing, Gary A. Heiman, Jay A. Tischfield, Peristera Paschou, A Jeremy Wilsey, Matthew W. State

Department of Biological Sciences Faculty Publications

We previously established the contribution of de novo damaging sequence variants to Tourette disorder (TD) through whole-exome sequencing of 511 trios. Here, we sequence an additional 291 TD trios and analyze the combined set of 802 trios. We observe an overrepresentation of de novo damaging variants in simplex, but not multiplex, families; we identify a high-confidence TD risk gene, CELSR3 (cadherin EGF LAG seven-pass G-type receptor 3); we find that the genes mutated in TD patients are enriched for those related to cell polarity, suggesting a common pathway underlying pathobiology; and we confirm a statistically significant excess of de novo …

Walking For Our Health: Couple-Focused Interventions To Promote Physical Activity In Older Adults, Melissa M. Franks, Elizabeth A. Richards, Meghan H. Mcdonough, Sharon L. Christ, Mary E. Marshall

School of Nursing Faculty Publications

More than 50% of U.S. adults do not engage in sufficient physical activity to meet current recommendations, making physical activity change and maintenance a priority for health promotion throughout adulthood. Among married partners, change in physical activity of one partner often is concordant with change of activity of the other. The primary purpose of this study was to examine two couple-focused interventions that capitalize on the co-occurrence of health behavior change within couples to promote physical activity in older adults. In this study, partners (N = 31 couples) participated together in assessment and intervention activities, and were randomized together into …

Mass Spectrometry Image Creator (Msic): Ion Mobility / Mass Spectrometry Imaging Workflow In Python, Stephen Creger, Julia Laskin, Daniela Mesa Sanchez

The Summer Undergraduate Research Fellowship (SURF) Symposium

Mass spectrometry (MS) is a powerful characterization technique that enables identification of compounds in complex mixtures. Acquiring mass spectra in a spatially-resolved manner (i.e. over a grid), allows the data to be used to generate images that show the spatial distribution and relative intensities of every compound in a sample. These images can be used to monitor and identify biomarkers, explore the metabolism of compounds within tissues, and much more. However, the limitations of mass spectrometry can result in ambiguous compound identifications. Another characterization tool, ion mobility spectrometry (IM) can be integrated into existing MS routines to address this problem; …

Cost-Effective Paper-Based Diagnostic Using Split Proteins To Detect Yeast Infections, Zachary R. Berglund, Kevin V. Solomon, Mohit S. Verma, Moiz Rasheed, Zachary Hartley, Kevin Fitzgerald, Kok Zhi Lee, Janice Chan, Julianne Dejoie, Makayla Schacht, Alex Zavala

The Summer Undergraduate Research Fellowship (SURF) Symposium

The common yeast infection, vulvovaginal candidiasis, affects three out of four women throughout their lifetime and can be spread to their child in the form of oral candidiasis (thrush). This disease is caused by the fungal pathogen Candida albicans, which is also a major cause of systemic candidiasis, a rarer but deadly disease with up to a 49% lethality rate. Current widely-used diagnostic methods include cell cultures, pH tests, and antibody detection, to assist effective treatment. Despite availability of various diagnostic methods, there is no inexpensive, rapid, and accurate way to detect C. albicans infection. This project aims to …

Polyrotaxane Variants And Their Effects On The Cholesterol Efflux In Patients Suffering From Niemann Pick Type C, Sydney Smith, Zach Struzik, David Thompson

The Summer Undergraduate Research Fellowship (SURF) Symposium

Niemann Pick type C disease (NPC) is a rare lysosomal storage disorder characterized by a progressive accumulation of cholesterol in the late endosomal/lysosomes compartment leading to cellular dysfunction and organ failure. Symptoms include ataxia, dysarthria, cognitive dysfunction, and seizures. Although average life expectancy is below 20, there are no FDA approved treatment available making it a serious unmet medical need. Clinical trials with 2-hydroxypropyl-beta-cyclodextrin (HP-beta-CD) has shown promise in cholesterol normalization within NPC cells. However, HP-beta-CD treatment has been shown to cause ototoxicity in NPC patients at high dosages. Supramolecular complexes known as polyrotaxanes have been synthesized in hopes of …

Targeting Pro-Inflammatory Function Of Microglia Using Small Molecules To Combat Neurodegeneration, Gabrielle C. Williams, Priya Prakash, Gaurav Chopra

The Summer Undergraduate Research Fellowship (SURF) Symposium

Microglia are the brain’s resident immune cells that are responsible for maintaining homeostasis in healthy conditions. During injury or infection, resting microglia get activated and produce pro-inflammatory cytokines such as IL-1b, IL-1a, IL-6, etc. along with reactive oxygen species like nitric oxide (NO) to combat neuroinflammatory diseases such as Alzheimer’s disease (AD). Inflammation is characterized by the activation of resident-immune cells in the brain called microglia that respond to the eat-me signals released by the toxic amyloid beta peptides as well as the dying neurons in the microenvironment. Recent studies have shown that activated microglia induce neuronal death by secreting …

Tissue Clearing As A Mechanism To Identify Changes In Fibronectin Structure During Breast Cancer Metastasis, Maryam Nuru, Kelsey Hopkins, Luis Solorio

The Summer Undergraduate Research Fellowship (SURF) Symposium

With metastasis accounting for approximately 90% of breast cancer deaths and an alarming number of over 300,000 new breast cancer cases to be diagnosed by the end of 2018, there is growing need to understand the process of breast cancer. Changes in the extracellular matrix (ECM) of the tumor microenvironment play an essential role in this deadly tumor progression. Specifically, the glycoprotein fibronectin (FN), has been identified to be up-regulated in patients with worse clinical outcomes. During tumor progression fibronectin undergoes conformational changes that aid in metastatic dissemination. In order to analyze the dynamic changes in FN expression and evaluate …

Targeting Neuropeptides To Bone Fractures For Accelerated Healing, Nicholas A. Young, Jeffery J. Nielsen, Philip S. Low

The Summer Undergraduate Research Fellowship (SURF) Symposium

In patients over the age of 65 especially, bone fractures represent a significant disease burden. Non-invasive drug therapies are not available for bone fractures which represents a problem for this population. Vasoactive intestinal peptide (VIP) and Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP), two neuromodulator peptides in the glucagon superfamily, have demonstrated positive regulation of osteoblast proliferation and activity. Using acidic oligopeptides, we have developed ligands that target to and accumulate at fracture sites. These targeting ligands can be synthesized in sequence with bone anabolic peptides to minimize off target effects and increase potency at the fracture site to create safer and …

Changing Seasons: A Language Arts Curriculum For Healthy Aging, Denise Calhoun

Purdue University Press Book Previews

Effective communication enhances quality of life. In Changing Seasons: A Language Arts Curriculum for Healthy Aging,Denise Calhoun provides a language-based, interdisciplinary program to help older adults improve their communication skills. Each activity reveals new, creative, and fun ways to get individuals to speak, think, write, engage with others, and use their imagination. As the activities promote meaningful interactions and the creation of a stimulating environment, Changing Seasons underscores the importance of sustaining quality of life as we and those we love age.

Habitual Heat Exposure And Acclimatization Associated With Athletic Performance In The Multistage Marathon Des Sables, Leonidas G. Ioannou, Lydia Tsoutsoubi, Lars Nybo, Georgios I. Tsianos, Andreas D. Flouris

Journal of Human Performance in Extreme Environments

Introduction

The aim of this study was to investigate the impact of heat acclimatization on athletic performance during the 7-day Marathon des Sables (MdS) which takes place in the Sahara Desert.

Methods

Anonymous data for nationality and average running speed (km/h) of all runners who ran the MdS during the period 2000–2015 were collected from the official website of the race and other related websites. Average maximum temperature for each runner’s country during the month preceding the MdS was collected from www.weatherbase.com. Athletes were divided into two Torigin groups as follows: 25 to 15°C (i.e., cold countries) and 15 to …