Agronomy and Crop Sciences Commons^™

Comparison Of Transcript Profiles In Wild-Type And O2 Maize Endosperm In Different Genetic Backgrounds, Hongwu Jia, Dan Nettleton, Joan M. Peterson, Gricelda Vasquez-Carrillo, Jean-Luc Jannink, M. Paul Scott

Dan Nettleton

Mutations in the Opaque2 (O2) gene of maize (Zea mays L.) improve the nutritional value of maize by reducing the level of zeins in the kernel. The phenotype of o2 grain is controlled by many modifier genes and is therefore strongly dependent on genetic background. We propose two hypotheses to explain differences in phenotypic severity in different genetic backgrounds: (i) Specific genes are differentially (o2 vs. wild-type) expressed only in certain genotypes, and (ii) A set of genes are differentially expressed in all backgrounds, but the degree of differential expression differs in different backgrounds. To determine …

A Diallel Analysis Of A Maize Donor Population Response To In Vivo Maternal Haploid Induction I: Inducibility, Gerald N. De La Fuente, Ursula K. Frei, Benjamin Trampe, Daniel Nettleton, Wei Zhang, Thomas Lubberstedt

Dan Nettleton

The maize in vivo maternal doubled haploid (DH) system is an important tool used by maize breeders and geneticists around the world. The ability to rapidly produce DH lines of maize for breeding allows breeders to quickly respond to new selection criteria based on the ever changing biotic and abiotic stresses that maize is subjected to across its growing area. There are two important steps in the generation of DH lines using the in vivo maternal DH system: 1) the production and identification of haploid progeny, and 2) the doubling of genomes to create fertile, diploid inbred lines that can …

A Clade-Specific Arabidopsis Gene Connects Primary Metabolism And Senescence, Dallas C. Jones, Wenguang Zheng, Sheng Huang, Chuanlong Du, Xuefeng Zhao, Ragothaman M. Yennamalli, Taner Z. Sen, Dan Nettleton, Eve S. Wurtele, Ling Li

Dan Nettleton

Nearly immobile, plants have evolved new components to be able to respond to changing environments. One example is Qua Quine Starch (QQS, AT3G30720), an Arabidopsis thaliana-specific orphan gene that integrates primary metabolism with adaptation to environment changes. SAQR (Senescence-Associated and QQS-Related, AT1G64360), is unique to a clade within the family Brassicaceae; as such, the gene may have arisen about 20 million years ago. SAQR is up-regulated in QQS RNAi mutant and in the apx1 mutant under light-induced oxidative stress. SAQR plays a role in carbon allocation: overexpression lines of SAQR have significantly decreased starch content; …

Soil Quality And Region Influence Performance And Ranking Of Switchgrass Genotypes, M. D. Casler, S. Sosa, A. R. Boe, S. A. Bonos

Arvid Boe

Development of switchgrass (Panicum virgatum L.) as a dedicated bioenergy feedstock requires intensive and extensive breeding programs that include careful and thoughtful consideration of appropriate target populations of environments (TPEs). The purpose of this study was to evaluate region (climate), soil quality, and N fertilization level as potential factors influencing the choice of TPE. A total of 45 switchgrass genotypes were evaluated in uniform field studies at six field sites defined as prime or marginal soils in New Jersey, South Dakota, and Wisconsin. Region and soil quality had strong interactions with genotype, but N fertilization had little impact on …

Native Grass And Legume Biology And Establishment, Arvid A. Boe, P. J. Johnson

Arvid Boe

No abstract provided.

Biomass Yield Of Switchgrass Cultivars Under High- Versus Low-Input Conditions, Michael D. Casler, Sergio Sosa, Lindsey Hoffman, Hilary Mayton, Calvin Ernst, Paul R. Adler, Arvid R. Boe, Stacy A. Bonos

Arvid Boe

Switchgrass (Panicum virgatum L.) is undergoing development as a biomass crop to support conversion of cellulosic biomass to energy. To avoid the competition of biomass with food or feed crops, most commercialization proposals suggest that switchgrass should be grown exclusively on marginal lands that are not fit for food or feed production. The objective of this study was to investigate the potential for cultivar x environment interactions that would affect the methods and approaches for breeding and evaluating switchgrass cultivars, including both upland and lowland types, for high-input versus low-input types of environments. Biomass yield was measured on 14 cultivars …

30 Years Of Progress Toward Increased Biomass Yield Of Switchgrass And Big Bluestem, M. D. Casler, K. P. Vogel, D. K. Lee, R. B. Mitchell, P. R. Adler, R. M. Suic, K. D. Johnson, R. L. Kallenbach, A. R. Boe, R. D. Mathison, K. A. Cassida, D. H. Min, J. Crawford, K. J. Moore

Arvid Boe

Breeding to improve biomass production of switchgrass (Panicum virgatum L.) and big bluestem (Andropogon gerardii Vitman) for conversion to bioenergy began in 1992. The purpose of this study was (i) to develop a platform for uniform regional testing of cultivars and experimental populations for these species, and (ii) to estimate the gains made by breeding during 1992 to 2012. A total of 25 switchgrass populations and 16 big bluestem populations were planted in uniform regional trials at 13 locations in 2012 and 2014. The reference region was USDA Hardiness Zones 3 through 6 in the humid temperate United …

Using Drones For Precision Agriculture, Jiyul Chang, Madhav P. Nepal

Madhav Nepal

In this teaching module, students will learn what Precision Agriculture is and how to apply drone into Precision Agriculture practices. To use data (images) taken by drone, students will learn the basic theory of Remote Sensing. Using images, students learn how to make NDVI (Normalized Difference Vegetation Index) maps and how to apply drone (remote sensing technique) in agriculture.

Nitrogen Fertilizer Suppresses Mineralization Of Soil Organic Matter In Maize Agroecosystems, Navreet K. Mahal, William R. Osterholz, Fernando E. Miguez, Hanna J. Poffenbarger, John E. Sawyer, Daniel C. Olk, Sotirios V. Archontoulis, Michael J. Castellano

John E. Sawyer

The possibility that N fertilizer increases soil organic matter (SOM) mineralization and, as a result, reduces SOM stocks has led to a great debate about the long-term sustainability of maize-based agroecosystems as well as the best method to estimate fertilizer N use efficiency (FNUE). Much of this debate is because synthetic N fertilizer can positively or negatively affect SOM mineralization via several direct and indirect pathways. Here, we test a series of hypotheses to determine the direction, magnitude, and mechanism of N fertilizer effect on SOM mineralization and discuss the implications for methods to estimate FNUE.Wemeasured the effect of synthetic …

Rethinking Invasion Impacts Across Multiple Field Sites Using European Swallowwort (Vincetoxicum Rossicum) As A Model Invader, Grant L. Thompson, Terrence H. Bell, Jenny Kao-Kniffin

Grant Thompson

Quantifying The Benefits Of On-Farm Best Management Practices For Irrigation And Nutrient Management, I. Zaragoza, K. Post, L. Johnson, R. Spellenberg, S. Kortman, C. Rosevelt, G. Muro, D. Goorahoo, F. Cassel-Sharma, M. Cahn, A. Haffa, F. Melton

Arlene Haffa

Impact Of Nitrogen Application Timing On Corn Production, John E. Sawyer, Daniel W. Barker, John P. Lundvall

John E. Sawyer

Water quality issues have renewed interest in timing of nitrogen (N) application as a means to improve use efficiency in corn and reduce losses. Improved economic return is also desired as N fertilization is one of the most costly inputs to corn production. Time of fertilizer application is a component of the site-specific 4R nutrient management stewardship programs. In Iowa, the Nutrient Reduction Strategy has a 7% (37% std. dev.) nitrate-N reduction with a 0% (3% std. dev.) corn yield change for sidedress compared to pre-plant N application (SP 0435A).

Crop Responses To Amisorb In The North Central Region, Sylvie Brouder, Robert Dowdy, Dave Franzen, Gary Hergert, Robert Hoeft, Maurice Horton, Keith Kelling, David Mengel, George Rehm, John Sawyer, Peter Scharf, Darryl Warnacke, David Whitney

John E. Sawyer

Originally used to prevent scale in boilers, carpramid or thermal polyaspartate (copoly[(3-carboxypropionamide)( 2-carboxylmethyl) acetamide)] was brought to agriculture under the trade names AmiSorb and Magnet. It claimed to increase nutrient uptake through artificially increasing the volume of soil occupied by roots through increased root branching and root hair development. Under controlled hydroponic or greenhouse conditions, the use of carpramid increased nutrient uptake, some yield determining factors such as wheat tillering and in some cases, crop yield. Extensive field testing from 1996 to 1998 under various nutrient regimes, placements, forms, and timings resulted in very inconsistent performance. Averaged across all experiments …

Nitrogen Fertilizer Rate Effects On Soil Organic Carbon In Iowa Continuous Corn And Corn-Soybean Systems, Hanna J. Poffenbarger, Daniel W. Barker, Matthew J. Helmers, Fernando E. Miguez, John E. Sawyer, Johan Six, Michael J. Castellano

John E. Sawyer

Nitrogen fertilizer rate is a key factor affecting soil organic C (SOC) in corn-based cropping systems. The objective of this study was to determine the change in SOC in response to long-term N rates for continuous corn and corn-soybean cropping systems at two sites in Iowa. Soil samples were collected to a depth of 15 cm in 1999 and again in 2014 after 15 years of corn N rate treatments ranging from 0 to 269 kg ha^-1. The soil samples were analyzed for total C and N concentrations. For continuous corn at both sites, the average annual change …

Genomic Approaches For Improvement Of Understudied Grasses, Keenan Amundsen, Gautam Sarath, Teresa Donze-Reiner

Teresa Donze-Reiner

No abstract provided.

A Smartphone-Based Device For Measuring Soil Organic Matter, Meng Lu

Meng Lu

The project evaluated the potential of utilizing a smartphone-based system for the in-field analysis of Soil Organic Matter. Although it demonstrated that the performance of the smartphone-based spectrometer can be comparable to commercial spectrometers, the results suggest that it is challenging to identify the spectral “signatures” of the SOM due to the morphology and moisture variation of soil samples.

Winter Grazing Management, Stephen K. Barnhart, James R. Russell, Douglas L. Karlen, Michael J. Tidman

Douglas L Karlen

Why winter grazing? Beef cow herd and sheep flock records show that winter feeding costs are livestock producers' single largest production expense. Managing through winter weather while keeping feeding costs low is an essential part of maintaining a profitable operation. Iowa's climate generally allows forage growth only during a 7-to-8 month period. Extending the grazing of this forage--even an extra 3 or 4 weeks in late autumn and winter--is an economical way to maintain or increase livestock profitability. Some producers extend the grazing season by using stockpiled forage, whereas others use crop residue, and many combine the use of stockpiled …

Winter Rye Cover Crop Biomass Production, Degradation, And N Recycling, J. L. Pantoja, J. E. Sawyer, D. W. Barker

John E. Sawyer

Winter rye (Secale cereale L.) as a cover crop can take up residual inorganic N between annual row crops and therefore be used to help reduce NO3 -–N loss from fields and movement to water systems. However, does the rye N uptake affect N recycling to soil and add to plant available N? The rye carbon:nitrogen (C:N) ratio could also influence N recycling. The objectives of this study were to evaluate rye biomass degradation and N recycling after spring rye termination in a no-till corn (Zea mays L.) - soybean [Glycine max. (L.) Merr.] rotation. A two year experiment (2010-2011) …

In-Season Nitrogen Fertilization Of Soybean, J. E. Sawyer, D. W. Barker

John E. Sawyer

Nitrogen (N) fertilization is not a traditional nutrient management practice for soybean production in Iowa. Soybean is a legume plant and is assumed to adequately obtain needed N through symbiotic fixation. However, there has been interest over the years in using N fertilization to increase yield and grain protein due to the recognition of the large N requirement associated with high yields. Depending on the soil residual inorganic N level and soil N mineralization characteristics, approximately 40 to 75% of the N in a mature soybean plant is derived from the soil (Shibles, 1998). Also, soybean seems to require this …

Corn And Soybean Response To Sulfur Application On Iowa Soils, J. E. Sawyer, D. W. Barker

John E. Sawyer

Historically sulfur (S) application has not been recommended on Iowa soils for com and soybean production. Prior research has not determined a consistent need for S fertilization in Iowa, with field research indicating no com or soybean yield response to applied S at virtually every site studied (Thorup and Leitch 1975; Webb, 1978; Alesii 1982; Killorn, 1984; Sexton et al., 1998; Mallarino et al., 2000). The soil supply, in combination with sources such as manure and atmospheric deposition has apparently met com and soybean S needs. Sulfur deficiencies have been reported over the years in various areas of the Midwestern …

Corn Nitrogen Rate Response And Crop Yield In A Rye Cover Crop System, John E. Sawyer, Jose L. Pantoja, Daniel W. Barker

John E. Sawyer

Water quality impairment related to N loss from crop production fields continues to be a concern in Iowa, including meeting the USEPA nitrate-N drinking water standard and reducing N export to the Gulf of Mexico. Therefore, in-field production practices would be helpful to aid in reduction of nitrate leaching and movement to water systems. One practice identified in the science assessment for the Iowa Nutrient Reduction Strategy is use of a winter cereal rye (Secale cereal L.) cover crop, where a mean 31% (29% standard deviation) nitrate-N reduction would be expected, but with a 6% (22% standard deviation) corn yield …

Dealing With Sulfur Deficiencies In Crop Production: The Iowa Experience, J. E. Sawyer, B. Lang, D. W. Barker, G. Cummins

John E. Sawyer

Research conducted for more than forty years (prior to approximately 2005) in Iowa rarely noted improved crop yield with sulfur (S) fertilization. Studies during that time period with corn and soybean found yield increase from S fertilizer application only three times out of approximately 200 trials. Research in the early 1980’s had also documented sufficient plant available S in the soil profile for crop production on most Iowa soil associations. Results of recent studies (2000- 2005) in corn and soybean were consistent with the historical research. An example is research presented at this conference (Sawyer and Barker, 2002) where there …

Nutrient Management Plan, Antonio Mallarino, John Sawyer, Barbara Stewart, John Creswell

John E. Sawyer

Management tools to help you develop a nutrient management plan and use the Iowa Phosphorus Index.

Nitrogen Application, John Sawyer, John Creswall

John E. Sawyer

Nitrogen (N) management planning for corn production.

Maximum Soil Organic Carbon Storage In Midwest U.S. Cropping Systems When Crops Are Optimally Nitrogen-Fertilized, Hanna J. Poffenbarger, Daniel W. Barker, Matthew J. Helmers, Fernando E. Miguez, Daniel C. Olk, John E. Sawyer, Johan Six, Michael J. Castellano

John E. Sawyer

Nitrogen fertilization is critical to optimize short-term crop yield, but its long-term effect on soil organic C (SOC) is uncertain. Here, we clarify the impact of N fertilization on SOC in typical maize-based (Zea mays L.) Midwest U.S. cropping systems by accounting for site-to-site variability in maize yield response to N fertilization. Within continuous maize and maize-soybean [Glycine max (L.) Merr.] systems at four Iowa locations, we evaluated changes in surface SOC over 14 to 16 years across a range of N fertilizer rates empirically determined to be insufficient, optimum, or excessive for maximum maize yield. Soil organic C balances …

Seasonal And Rotational Influences On Corn Nitrogen Fertilization In Southwest Iowa, John Sawyer, Daniel Barker

John E. Sawyer

This project was designed to study the nitrogen (N) fertilization needs in continuous corn (CC) and corn rotated with soybean (CS) as influenced by location and climate. Multiple rates of fertilizer N were spring applied, with the intent to measure yield response to N within each rotation on a yearly basis for multiple years at multiple sites across Iowa. This will allow determination of N requirements for each rotation, differences that exist between the two rotations, responses to applied N across different soils and climatic conditions, and evaluation of tools used to adjust N application.

Enhancing Corn Yield In A Winter Cereal Rye Cover Crop System In Southwest Iowa, John Lundvall, Swetabh Patel, John Sawyer

John E. Sawyer

Water quality impairment related to nitrogen (N) is a concern in Iowa, including meeting nitrate (NO3) drinking water standards and reducing the amount of N lost to the Gulf of Mexico. The Iowa Nutrient Reduction Strategy science assessment identified a rye cover crop as an important in-field management practice for reducing N and phosphorus (P) loss from fields (31% NO3-N and 29% P), and for reducing soil erosion. However, the science assessment identified a corn yield reduction of 6 percent when grown following a rye cover crop. Lower corn yield with use of a cover crop is unacceptable to farmers, …

Corn Nitrogen Fertilization Requirement And Corn–Soybean Productivity With A Rye Cover Crop, Jose L. Pantoja, Krishna P. Woli, John E. Sawyer, Daniel W. Barker

John E. Sawyer

Winter rye (Secale cereale L.) cover crop (RCC) has potential to reduce NO₃–N loss from corn (Zea mays L.) and soybean [Glycine max (L.) Merr.] fields. However, RCC effects on annual crop productivity and corn optimal N fertilization requirement are unclear. The objectives were to evaluate corn and soybean yield response to RCC and corn optimal N rate. Treatments were no-RCC and RCC with six fertilizer N rates (0–225 kg N ha^-1) applied to corn in a no-till corn–soybean (CS) rotation at four Iowa sites in 2009 through 2011. The RCC biomass and …

Standardized Research Protocols Enable Transdisciplinary Research Of Climate Variation Impacts In Corn Production Systems, E. J. Kladivko, M. J. Helmers, L. J. Abendroth, D. Herzmann, R. Lal, M. J. Castellano, D. S. Mueller, J. E. Sawyer, R. P. Anex, R. W. Arritt, B. Basso, J. V. Bonta, L. C. Bowling, R. M. Cruse, N. R. Fausey, J. R. Frankenberger, P. W. Gassman, A. J. Gassmann, C. L. Kling, A. Kravchenko, J. G. Lauer, F. E. Miguez, E. D. Nafziger, N. Nkongolo, M. O'Neal, L. B. Owens, P. R. Owens, P. Scharf, M. J. Shipitalo, J. S. Strock, M. B. Villamil

John E. Sawyer

The important questions about agriculture, climate, and sustainability have become increasingly complex and require a coordinated, multifaceted approach for developing new knowledge and understanding. A multistate, transdisciplinary project was begun in 2011 to study the potential for both mitigation and adaptation of corn-based cropping systems to climate variations. The team is measuring the baseline as well as change of the system's carbon (C), nitrogen (N), and water footprints, crop productivity, and pest pressure in response to existing and novel production practices. Nine states and 11 institutions are participating in the project, necessitating a well thought out approach to coordinating field …

Why Manage Phosphorus?, John E. Sawyer, Antonio P. Mallarino

John E. Sawyer

There are 17 nutrients that are essential for plant growth and production. An insufficient supply of one or more can have an adverse effect on plant growth, maturity, and yield. Phosphorus (P)--one of three key macronutrients (nitrogen and potassium are the other two)--is present in every living plant and animal cell, and is vital in plants for harvesting the sun's energy for growth and reproduction. This requirement is the same whether the plant is growing on soil or in water (such as algae); hence, the concern regarding P levels in surface water and the accelerated aquatic plant growth associated with …