Toxicology Commons^™

Microbial Phenol Degradation Utilizing A Complete-Mix Biological Reactor : The Effects Of Dissolved Oxygen Content, Keith Kollar

Theses

Experiments were conducted using phenol as a sole carbon source in a series of completely mixed biological reactors with solids recycle (CMBR). The reactor working volume was 4 liters, and solids were recycled from 3 liter clarifiers. Dissolved oxygen concentration (DO) was varied in order to determine the impact of this important variable on system operability.

Phenol was removed at better than 99 percent efficiency during most of the runs. Filamentous growth was not observed during any run. However, bulking did occur at higher DO levels, which was the result of microbial slime production.

Comparative Adsorption Studies On Clay Soils, Prasanna R. Mysore

Theses

An investigation has been made to study clay as sorbent in the treatment of the hazardous contaminants of potential sludge leachate emanating from industrial landfills.

Experimentally was studied six highly toxic and hazardous chemicals, which include: phenol, aniline, cyclohexanol, cyclohexanone, cresol and monochlorophenol. Vermiculate, Kaolinite, Attacote and Hectorite clays were studied as the sorbent material. Removal of organic pollutants were measured using a total organic carbon analyzer (TOC).

Results indicate that these clays can be used for some treatment of the above mentioned organic compounds. The study shows that removal of organic pollutants by clay depends on several factors, such …

Reactions Of Organic N-Chloramines In The Gastric Fluid Of The Rat, Kathryn E. Mazina

Chemistry & Biochemistry Theses & Dissertations

Using chlorine as a drinking water disinfectant may have potential health effects due to its reactivity with organic amino nitrogen compounds found in the stomach. Organic N-chloramines have been shown to form in the stomachs of laboratory rats. The possible reactions of N-chloramines in the stomach fluid were examined in this study using a model radiolabeled N-chloramine. ³⁶Cl-N-Chloropiperidine, was synthesized and purified to remove ³⁶Cl-chloride. Stomach fluid was obtained from Sprague-Dawley rats which had been first fasted for 24 or 48 hours and then administered 3 mL of deionized water. Different concentrations of radiolabeled chloramine were reacted with …

The Sunlight Effect On Trace Pollutants In Wastewater, Alan R. Sheu

Theses

In order to study the sunlight effect on the degradation rate of trace pollutants, ortho-cresol was selected to serve as a model compound in our studies. Solution of ortho-cresol alone, methylene blue alone, and the mixture of ortho-cresol and methylene blue were exposed to sunlight for more than four hours and aliquots analyzed with a spectrophotometer in order to follow the changes in absorption. Rate data were obtained for these three cases as a function of pH.

The results indicate that methylene blue does catalyze the degradation of ortho-cresol in the presence of sunlight with lower pH's affording higher reaction …

Indicators Of Microbial Activity In Biological Treatment Of Hazardous Wastes, Alexander Mclean Sherrin

Theses

Three methods were used to measure the activity of mixed liquor from the Livingston, N.J. municipal wastewater treatment plant on exposure to phenol. The three methods were, substrate removal rate (SRR), dissolved oxygen uptake rate (DOUR), and the dye reduction rate (DRR). Parameters from each test were compared for reproducibility and ease of determination. The SRR test gave the most reproducible results, while the DOUR test was the least reproducible. Although the reproducibility of the DRR test was close to that of the SRR test, the DRR was more time consuming and required a greater degree of expertise and training. …

Fish Acute Toxicity Syndromes And Their Use In The Qsar Approach To Hazard Assessment, Steven P. Bradbury, James M. Mckim, Gerald J. Niemi

Steven P. Bradbury

Implementation of the Toxic Substances Control Act of 1977 creates the need to reliably establish testing priorities because laboratory resources are limited and the number of industrial chemicals requiring evaluation is overwhelming. The use of quantitative structure activity relationship (QSAR) models as rapid and predictive screening tools to select more potentially hazardous chemicals for in-depth laboratory evaluation has been proposed. Further implementation and refinement of quantitative structure-toxicity relationships in aquatic toxicology and hazard assessment requires the development of a "mode-of-action" database. With such a database, a qualitative structure-activity relationship can be formulated to assign the proper mode of action, and …

Physiological Response Of Rainbow Trout (Salmo Gairdneri) To Acute Fenvalerate Lntoxication, Steven P. Bradbury, James M. Mckim, Joel R. Coats

Steven P. Bradbury

The physiological responses of rainbow trout (Salmo gairdneri) to fenvalerate intoxication during aqueous exposure were examined to provide information about the pyrethroid mode of action in fish. Trout (n = 4) were exposed to 412 ± 50 μg/liter fenvalerate and died in 10.9 ± 1.5 hr. Brain, liver, and carcass fenvalerate concentrations associated with mortality were 0.16 ± 0.05, 3.62 ± 0.57, and 0.25 ± 0.05 mg/kg, respectively. Visible signs of intoxication included elevated cough rate, tremors, and seizures. Histopathological examination of gill tissue showed damage consistent with irritation. An evaluation of respiratory-cardiovascular and blood chemistry responses indicated an elevated …

Characterization Of Microorganisims After Feeding With Phenolic Compounds : Comparing A Domestic Sludge To An Industrial Sludge, Dianne Adamowitz

Theses

This study examined the effects of phenolic compounds on the populations in mixed liquors obtained from two very different treatment plants: the Passaic Valley Sewerage Commissioners plant in Newark, and the Livingston, NJ municipal treatment plant. The dominant organisms were identified initially and after ten days exposure to phenol (100 ppm), and either 2-chlorophenol (20 ppm) or 2,6-dichlorophenol (10 ppm) in aerated batch reactors.

Results confirmed the remarkable similarity between the two mixed liquor populations, in spite of the significant differences in the operation of the two treatment plants.

Analysis Of The Charleston, Illinois Water Supply For The Presence Of Organic Mutagens Utilizing The Ames Salmonella/Microsome Assay, Steven Lee Washburn

Masters Theses

Water samples from the Charleston, Illinois water supply and selected agricultural run-off areas were tested for the presence of organic mutagenic chemicals. The Ames Salmonella/microsome mutagenicity assay utilizing strains TA98 and TA100 was used to analyze concentrated water samples. A special set of samples was exposed to the S9 activation system coupled with the TA98 strain to simulate the mammalian metabolic system.

No mutagens were detected in any of the samples tested. Several positive and negative control systems were included in the testing system. Negative control systems were used to determine the average spontaneous reversion rates for each strain for …

Microbial Characteristics Of An Industrial Mixed Liquor After Exposure To Phenolic Compounds, Patricia L. Boyle

Theses

The microbial characteristics of a mixed population from the Passaic Valley Sewerage Commissioners (PVSC) Wastewater Treatment Plant in Newark were examined. This plant handles about 250 million gallons per day of mixed sewerage, with a large industrial component. The microbial species were characterized using standard plating techniques, morphology, microscopic observation, and biochemical tests. These characteristics were determined for the fresh liquor, and after successive batch exposure to phenol (100 ppm) and 2-chlorophenol (20 ppm). Predator/prey, gram positive to gram negative bacteria ratios, and the fungi and protozoan populations were also determined. These results were compared with those previously obtained using …

Toxicological Studies On Aquatic Contaminants Originating From Coal Production And Utilization: The Induction Of Tolerance To Silver In Laboratory Populations Of Fish And The Chronic Toxicity Of Nickel To Fish Early Life Stages, Wesley J. Birge, Jeffrey A. Black, James F. Hobson, Albert G. Westerman

KWRRI Research Reports

Aquatic toxicity studies were performed on two important coal-derived contaminants, silver and nickel. Silver was investigated with regard to metal-induced tolerance in laboratory populations of the fathead minnow (Pimephales promelas). Fish were exposed to acute silver concentrations following acclimation to sublethal exposures of this metal. Based on median lethal times (LT50), animals which had received 14 days prior exposure to 1.5 and 15 μg Ag/L were three to four times more resistant to silver than were previously unexposed organisms. This metal-induced resistance was not a sustained response. After organisms which had been acclimated to 15 μg/L had been transferred to …

Evaluation Of Carbon-14 Uptake Algal Toxicity Assay And Its Application In Field Assessment Of Tributyltin Chloride And Chlorinated Sewage Toxicities, Soon Lin Ho

Dissertations, Theses, and Masters Projects

No abstract provided.

The Induction Of Tolerance To Heavy Metals In Natural And Laboratory Populations Of Fish, Wesley J. Birge, William H. Benson, Jeffrey A. Black

KWRRI Research Reports

Aquatic toxicity studies were performed on two natural populations of fathead minnows. One group of organisms was taken from a metal-contaminated flyash pond associated with a coal-fired power plant and the other group was collected from relatively uncontaminated hatchery ponds. Acute tests indicated that flyash pond fish were significantly more tolerant to cadmium and copper than were hatchery fish. At an exposure concentration of 6.0 mg Cd/L in moderately hard water, the median period of survival for flyash pond fish was 50.0 hr compared to 6.8 hr for hatchery fish. Both groups of organisms were about equally sensitive to zinc. …

Residual Phytotoxicity Of Mon-097 In Sludge Enriched Soils, Jennifer A. Woods

Masters Theses

Aerobically and anaerobically digested sludges were added to soil at concentrations of 0, 1, 5, and 10% (v/v). Dry weights of Avena sativa var. Noble plants, harvested 28 days after planting, were greater with increased sludge concentrations. Plant dry weights were consistently greater with aerobically than with anaerobically digested sludge. MON-097, an acetanilide herbicide, was applied to these soil/sludge mixtures at concentrations of 0, 0.5, 1, 2, 4, 8, 16 and 32 ppm. Residual phytotoxicity of MON-097 was not significantly affected by sludge concentration. These results suggest that increased concentrations of sludge in the soil do not contribute to detoxification …

Effects Of Organic Compounds On Amphibian Reproduction, Wesley J. Birge, Jeffrey A. Black, Robert A. Kuehne

KWRRI Research Reports

Aquatic toxicity tests were conducted with atrazine, carbon tetrachloride, chloroform, methylene chloride, trisodium nitrilotriacetic acid (NTA), and phenol. Each compound was administered to developmental stages of three to five amphibian species. Exposure was initiated at fertilization and maintained through 4 days posthatching. Test responses included lethality and teratogenesis. Different amphibian species exhibited varying degrees of tolerance to the selected compounds. Greatest tolerance usually was observed for the more broadly adapted semi-aquatic and terrestrial species (e.g., Bufo americanus, Bufo fowleri). The more sensitive amphibians usually included those species which normally are restricted to aquatic or moist habitats (e.g., Rana …

Uptake, Distribution, Metabolism And Clearance Of Kepone By Channel Catfish (Ictalurus Punctatus), Peter A. Vanveld

Dissertations, Theses, and Masters Projects

No abstract provided.

Effects Of Ingestion Of Kepone Contaminated Food By Juvenile Blue Crabs (Callinectes Sapidus Rathbun), Daniel J. Fisher

Dissertations, Theses, and Masters Projects

No abstract provided.

Metals In Apple Cider Produced And Marketed In Connecticut, Dennis W. Hill, Thomas R. Kelley, Gale R. Morrow, Sylvia W. Matiuck

Storrs Agricultural Experiment Station

No abstract provided.

The Distribution Of Cadmium, Chromium And Lead In Crabs, Clams And Oysters From Calcasieu Estuary, Louisiana, Fritz Jaenike

Masters Theses

In November 1978, specimens of oysters (Crassostrea virginica), clams (Rangia cuneata), crabs (Callinectes sapidus) and water and sediment samples were collected from Calcasieu Estuary, Louisiana. The concentrations of cadmium, chromium and lead were determined in the samples with an inductively coupled argon plasma direct reading emission spectrophotometer. Crab claw muscle, gill and carapace tissues were analyzed separately. Oysters and clams were separated into shell and whole soft tissue samples which were analyzed separately. The concentration order of the metals in crab carapace and in oyster shells was similar to that of the sediments, (Pb …

Removal Of Phosphorus From Static Sewage Effluent By Waterhyacinth, W Harold Ornes

W. Harold Ornes

Waterhyacinth [Eichhornia crassipes (Mart.) Solms] was grown in static sewage effluent during May to July 1974 in outdoor concrete containers with a capacity of 760 liters and a surface area of 1.66 m². The plants were removed weekly from one-half of the surface area of the containers during 5-wk growth periods. Tissue phosphorus (P) and nitrogen (N), plant productivity, and some parameters of water quality were measured. A maximum uptake of 5,500 µg of P/g dry weight of plant material occurred when the level of orthophosphate phosphorus (available P) in the effluent was 1.1 µg/ml. Phosphorus in the effluent was …

Pesticide Effects In A Simulated Soil Ecosystem, Raymond J. Samp

Masters Theses

The screening of pesticides to determine which were most inhibitory to bacteria was accomplished by testing the effects of 10 pesticides on 9 different organisms using the disk assay method. Results showed that Gram positive bacteria were more sensitive to all pesticides tested than were the Gram negative bacteria. In addition, the hormone herbicides were found to be the most inhibitory to these bacteria and 2,4-D (2,4-dichlorophenoxyacetic acid), a hormone herbicide, was chosen for subsequent in vivo studies.

A soil perfusion apparatus was used to determine the effects of 2,4-D in a typical soil ecosystem. Bacterial plate counts and nutrient …

Acute Effect Of Free Chlorine On Selected Estuarine Invertebrates And Vertebrates : Final Report, Morris H. Roberts, Robert J. Diaz

Reports

The objective of this project was to determine acute toxic effects of chlorine on selected estuarine organisms found adjacent to the projected outfall of a sewage treatment plant in the lower York River. The test species specified under contract were ovster (Crassostrea virginica) and clam (Mercenaria mercenaria) larvae, Acartia tonsa (a dominant copepod), and the fishes, menhaden (Brcvoortia tyranus), pipefish (Svngnathus fuscus), blennies (Hypsoplennius hentzi) and sheepshead minnow (Cyprinodon variegatus). The parameter of interest in the tests was mortality when the animals were exposed to a constant level of chlorine for a 48 or 96 hr period.

A Study Of Organochlorine Insecticides In Freshwater Crayfish -- Analytical Problems And Biomonitoring Survey, Robert C. Vanderjack

Masters Theses

Crayfish were collected from 3 sites on Polecat Creek, Coles County, Illinois in early spring, early summer, and late summer of 1972. Pesticides were extracted from the crayfish tissue in a one step procedure utilizing a Florisil elution column. Analysis was by electron capture gas chromatography. Pesticide residues in the nannogram range were analyzed and background contamination was an important consideration. Sources of background contamination were investigated and teflon stopcocks, Florisil, and residues on “cleaned” glassware were found to be major contamination sources. Distilled water used in flushing glassware should also be considered a potential contamination source.

Aldrin, dieldrin, and …

Acute Toxicity Of No 6 Fuel Oil To Intertidal Organisms In The Lower York River, Virginia, Jeffrey L. Hyland

Dissertations, Theses, and Masters Projects

No abstract provided.

Interbasin Movement Of Ground Water At The Nevada Test Site, Isaac J. Winograd

Publications (WR)

The present paper presents hydraulic evidence for the interbasin circulation of ground water through carbonate rocks of Paleozoic age at the Nevada Test Site. An integral part of this evidence is the discovery that aquifers in alluvium and tuff, formerly thought to be the principal aquifers at the Test Site, are semiperched above a thick tuffaceous aquiclude that separates them from the carbonate rocks.

This paper is based on one of the studies being made by the Geological
Survey for the Atomic Energy Commission. These studies seek to evaluate
the risk that may arise if ground water should be contaminated …

The Water Supply Of Minneapolis, James A. Dodge, C. L. Herrick, C. W. Hall

Journal of the Minnesota Academy of Science

No abstract provided.