Nutrition Commons^™

Uracil Accumulation In Folate Depleted Mouse Embryonic Fibroblasts, Eneida Doko

Wayne State University Theses

Folate is a water-soluble vitamin B that plays a critical co-enzyme in the de novo nucleotide synthesis and other biochemical processes including DNA metabolism, DNA repair, DNA methylation, and cellular growth. Folate deficiency has been associated to increase the risk of neural tube defects (NTDs) and cancers of the lung, breast, colon, cervix, esposphagus and brain. Most importantly, folate deficiency has been shown to increase uracil misincorporation into DNA and therefore induce DNA damage repaired by the base excision repair (BER) pathway. In response to folate depletion, levels of thmidylate decrease in the deoxyribonucelotide pool, resulting in uracil being misincorporation …

The Role Of Uracil-Dna Glycosylase And Folate In The Repair Of Dna, Sarah Talal Dubaisi

Wayne State University Theses

Abstract

Role of Uracil DNA-Glycosylase and Folate

In the Repair of Oxidative Damage

By

Sarah Dubaisi

May2012

Advisor: Dr. Diane Cabelof

Major: Nutrition and Food Science

Degree: Masters of Science

The impact of DNA damage on genomic integrity has been widely investigated due to its association with cancer and ageing. DNA repair pathways play a critical role in preserving the genomic integrity and protecting the cells against DNA damage caused by oxidative stress and folate deficiency. Base Excision Repair (BER) is one of the major pathways involved in the cellular response to DNA damage, primarily the damage caused by oxidative …

The Use Of Metabolomics To Investigate Biomarker Profiles As Potential Early Risk Factors For Development Of Type Ii Diabetes Mellitus, Jennelle Lynn Arnew (Getty)

Wayne State University Theses

ABSTRACT

THE USE OF METABOLOMICS TO INVESTIGATE BIOMARKERS PROFILES AS POTENTIAL EARLY RISK FACTORS FOR DEVELOPMENT OF TYPE II DIABETES

by

JENNELLE ARNEW

MAY 2012

Advisor: Dr. Smiti Gupta

Major: Nutrition and Food Science

Degree: Masters of Science

Type 2 Diabetes affects an estimated 17.5 million individuals in the United States and is considered to be one of the most pressing public health issues of our time. To date, the exact cause of type 2 diabetes remains unclear, however, it is considered to be an interplay between environmental and genetic factors. Emerging technologies for metabolomics analysis increases the capacity to …

Purification Of Alpha-Glucosidase Inhibitors From Grape Extract, Gayle M. Shipp

Wayne State University Theses

ABSTRACT

PURIFICATION OF ALPHA-GLUCOSIDASE INHIBITORS FROM GRAPE EXTRACT

by

GAYLE SHIPP

August 2012

Advisor: Dr. Kevin Zhou

Major: Nutrition and Food Science

Degree: Master of Science

Diabetes and its complications are steadily growing and remain major causes of morbidity and mortality in the U.S. Intestinal á-glucosidases play a crucial role in controlling postprandial blood glucose. For this reason, one attractive prevention and/or treatment strategy for type-2 diabetes is the inhibition of á-glucosidase. The effectiveness of á-glucosidase inhibitors (AGI's) for diabetes treatment is well documented in numerous animal and human clinical studies. Grape pomace extract (GPE) has recently found in our …

The Effects Of Oil Palm Phenolics On Inflammation And Oxidative Stress In Relation To Amyloid Beta Plaques In Fad-Mutant And Wild Type B103 Cells, Lindsay Michelle Godsey

Wayne State University Theses

Alzheimer's disease (AD) affects approximately 35.6 million people in the world today (35). It is expected that by year 2050, an individual will develop AD every 33 seconds (8). Unfortunately, the etiology is not well known. Familial Alzheimer's disease (FAD) occurs due to specific genetic mutations, making it possible to replicate them in cells. Using B103 rat neuroblastoma cells transfected with a cDNA construct, wild type cells and FAD mutant cells were used in this research.

Natural ingredients such as oil palm phenolics (OPP) contain antioxidants. After finding the optimal experimental parameters, OPP was found to aid in the destabilization …