Open Access. Powered by Scholars. Published by Universities.®
- Institution
-
- Himmelfarb Health Sciences Library, The George Washington University (14)
- Dartmouth College (10)
- Western University (9)
- Old Dominion University (8)
- University of Nebraska - Lincoln (8)
-
- Selected Works (5)
- Lawrence University (4)
- Munster Technological University (4)
- City University of New York (CUNY) (2)
- Embry-Riddle Aeronautical University (2)
- Missouri State University (2)
- Rowan University (2)
- The University of San Francisco (2)
- University of Arkansas, Fayetteville (2)
- University of Tennessee, Knoxville (2)
- California Polytechnic State University, San Luis Obispo (1)
- Central Washington University (1)
- Chapman University (1)
- Collin College (1)
- Duquesne University (1)
- East Tennessee State University (1)
- HCA Healthcare (1)
- Kansas State University Libraries (1)
- Karbala International Journal of Modern Science (1)
- Louisiana State University (1)
- Purdue University (1)
- Roseman University of Health Sciences (1)
- SIT Graduate Institute/SIT Study Abroad (1)
- South Dakota State University (1)
- Technological University Dublin (1)
- Keyword
-
- Virology (8)
- HIV (7)
- HIV-1 (7)
- Humans (6)
- Microbiology (6)
-
- Autophagy (5)
- Virus (5)
- 1.6 BIOLOGICAL SCIENCES (4)
- Animals (4)
- Biochemistry (4)
- Icosahedron (4)
- Mice (4)
- Nanobiology (4)
- Pathogen (4)
- Teaching (4)
- 3. MEDICAL AND HEALTH SCIENCES (3)
- 3.3 HEALTH SCIENCES (3)
- Biochemistry and molecular biology (3)
- Virus replication (3)
- Zika (3)
- Antiretroviral Therapy, Highly Active (2)
- Antiviral agents (2)
- Biology (2)
- COVID-19 (2)
- Coevolution (2)
- Complications (2)
- Dengue (2)
- Disease progression (2)
- Envelope (2)
- FIV (2)
- Publication Year
- Publication
-
- Dartmouth Scholarship (10)
- Electronic Thesis and Dissertation Repository (8)
- Microbiology, Immunology, and Tropical Medicine Faculty Publications (5)
- Chemistry & Biochemistry Theses & Dissertations (4)
- Department of Biological Sciences Publications (4)
-
- Electronic Theses and Dissertations (4)
- Infectious Pathogens (4)
- Nebraska Center for Virology: Faculty Publications (4)
- Celia A. Schiffer (3)
- Medicine Faculty Publications (3)
- Biological Sciences Theses & Dissertations (2)
- Environmental and Occupational Health Faculty Publications (2)
- Graduate Theses and Dissertations (2)
- MSU Graduate Theses (2)
- Pediatrics Faculty Publications (2)
- Publications (2)
- Undergraduate Honors Theses (2)
- All Faculty Scholarship for the College of the Sciences (1)
- Annual Research Symposium (1)
- Articles (1)
- Bioelectrics Publications (1)
- Biological Sciences Faculty Publications (1)
- Chancellor’s Honors Program Projects (1)
- Dissertations (1)
- Dissertations & Theses (Open Access) (1)
- Dissertations, Theses, and Capstone Projects (1)
- Doctoral Dissertations (1)
- Emergency Medicine Faculty Publications (1)
- Faculty Publications (1)
- Graduate School of Biomedical Sciences Theses and Dissertations (1)
- Publication Type
- File Type
Articles 91 - 105 of 105
Full-Text Articles in Virology
Direct Inhibition Of Cdk9 Blocks Hiv-1 Replication Without Preventing T Cell Activation In Primary Human Peripheral Blood Lymphocytes, Dominic Salerno, Muneer G Hasham, Renée Marshall Demarest, Judit Garriga, Alexander Y Tsygankov, Xavier Graña
Direct Inhibition Of Cdk9 Blocks Hiv-1 Replication Without Preventing T Cell Activation In Primary Human Peripheral Blood Lymphocytes, Dominic Salerno, Muneer G Hasham, Renée Marshall Demarest, Judit Garriga, Alexander Y Tsygankov, Xavier Graña
Rowan-Virtua School of Osteopathic Medicine Faculty Scholarship
HIV-1 transcription is essential for the virus replication cycle. HIV-1 Tat is a viral transactivator that strongly stimulates the processivity of RNA polymerase II (RNAPII) via recruitment of the cyclin T1/CDK9 positive transcription elongation factor, which phosphorylates the C-terminal domain (CTD) of RNAPII. Consistently, HIV-1 replication in transformed cells is very sensitive to direct CDK9 inhibition. Thus, CDK9 could be a potential target for anti-HIV-1 therapy. A clearer understanding of the requirements for CDK9 activity in primary human T cells is needed to assess whether the CDK9-dependent step in HIV-1 transcription can be targeted clinically. We have investigated the effects …
Aids-Associated Viral Oncogenesis: Overview, Charles Wood
Aids-Associated Viral Oncogenesis: Overview, Charles Wood
Nebraska Center for Virology: Faculty Publications
It has been 25 years since the acquired immunodeficiency syndrome (AIDS) was first described and over 23 years since the human immunodeficiency virus (HIV) associated with the disease was first discovered. In spite of the tremendous progress that was made in understanding both the disease and the virus, there are still millions of people infected, died, or living with the disease. As for the year 2005 alone, the Joint United Nations Programme on HIV/AIDS (http://www.UNAIDS.org) estimates that there are about 40 million people living with HIV/AIDS globally, and approximately 3 million people died from AIDS in the year. Globally, it …
The Role Of Cd4 T Cells In The Pathogenesis Of Murine Aids, Wen Li, William R. Green
The Role Of Cd4 T Cells In The Pathogenesis Of Murine Aids, Wen Li, William R. Green
Dartmouth Scholarship
LP-BM5, a retroviral isolate, induces a disease featuring retrovirus-induced immunodeficiency, designated murine AIDS (MAIDS). Many of the features of the LP-BM5-induced syndrome are shared with human immunodeficiency virus-induced disease. For example, CD4 T cells are critical to the development of MAIDS. In vivo depletion of CD4 T cells before LP-BM5 infection rendered genetically susceptible B6 mice MAIDS resistant. Similarly, MAIDS did not develop in B6.nude mice. However, if reconstituted with CD4 T cells, B6.nude mice develop full-blown MAIDS. Our laboratory has shown that the interaction of B and CD4 T cells that is central to MAIDS pathogenesis requires ligation of …
T-Cell Responses To The M3 Immune Evasion Protein Of Murid Gammaherpesvirus 68 Are Partially Protective And Induced With Lytic Antigen Kinetics, Joshua J. Obar, Douglas C. Donovan, Sarah G. Crist, Ondine Silvia, James P. Stewart, Edward J. Usherwood
T-Cell Responses To The M3 Immune Evasion Protein Of Murid Gammaherpesvirus 68 Are Partially Protective And Induced With Lytic Antigen Kinetics, Joshua J. Obar, Douglas C. Donovan, Sarah G. Crist, Ondine Silvia, James P. Stewart, Edward J. Usherwood
Dartmouth Scholarship
DNA vaccination with the M3 gene, encoding an immune evasion molecule expressed during both the acute lytic and persistent phases of murid gammaherpesvirus 68 infection, yielded a significantly lower titer of virus in the lung than controls. The protection seen was dependent on T cells, and we mapped an epitope recognized by CD8 T cells. The immune response to this epitope follows the same kinetics as lytic cycle antigens, despite the fact that this gene is expressed in both lytic and persistent stages of infection. This has important implications for our understanding of T-cell responses to putative latency-associated gammaherpesvirus proteins …
The Cd154/Cd40 Interaction Required For Retrovirus-Induced Murine Immunodeficiency Syndrome Is Not Mediated By Upregulation Of The Cd80/Cd86 Costimulatory Molecules, Kathy A. Green, W. James Cook, Arlene H. Sharpe, William R. Green
The Cd154/Cd40 Interaction Required For Retrovirus-Induced Murine Immunodeficiency Syndrome Is Not Mediated By Upregulation Of The Cd80/Cd86 Costimulatory Molecules, Kathy A. Green, W. James Cook, Arlene H. Sharpe, William R. Green
Dartmouth Scholarship
C57BL/6 (B6) mice infected with LP-BM5 retroviruses develop disease, including an immunodeficiency similar to AIDS. This disease, murine AIDS (MAIDS), is inhibited by in vivo anti-CD154 monoclonal antibody treatment. The similar levels of insusceptibility of CD40−/− and CD154−/− B6 mice indicate that CD154/CD40 molecular interactions are required for MAIDS. CD4+ T and B cells, respectively, provide the CD154 and CD40 expression needed for MAIDS induction. Here, the required CD154/CD40 interaction is shown to be independent of CD80 and CD86 expression: CD80/CD86−/− B6 mice develop MAIDS after LP-BM5 infection.
Human Exposure To Herpesvirus B–Seropositive Macaques, Bali, Indonesia, Gregory A. Engel, Lisa Jones-Engel, Michael A. Schillaci, Komang Gde Suaryana, Artha Putra, Agustin Fuentes, Richard Henkel
Human Exposure To Herpesvirus B–Seropositive Macaques, Bali, Indonesia, Gregory A. Engel, Lisa Jones-Engel, Michael A. Schillaci, Komang Gde Suaryana, Artha Putra, Agustin Fuentes, Richard Henkel
All Faculty Scholarship for the College of the Sciences
Herpesvirus B (Cercopithecine herpesvirus 1) has been implicated as the cause of approximately 40 cases of meningoencephalitis affecting persons in direct or indirect contact with laboratory macaques. However, the threat of herpesvirus B in nonlaboratory settings worldwide remains to be addressed. We investigated the potential for exposure to herpesvirus B in workers at a “monkey forest” (a temple that has become a tourist attraction because of its monkeys) in Bali, Indonesia. In July 2000, 105 workers at the Sangeh Monkey Forest in Central Bali were surveyed about contact with macaques (Macaca fascicularis). Nearly half of those interviewed had …
Construction And Characterization Of A Chimeric Virus (Biv/Hiv-1) Carrying The Bovine Immunodeficiency Virus Gag-Pol Gene: Research Letters, Guomin Chen, Shuhui Wang, Kun Xiong, Jinzhong Wang, Tao Ye, Wenping Dong, Qi Wang, Qimin Chen, Yunqi Geng, Charles Wood, Yi Zeng
Construction And Characterization Of A Chimeric Virus (Biv/Hiv-1) Carrying The Bovine Immunodeficiency Virus Gag-Pol Gene: Research Letters, Guomin Chen, Shuhui Wang, Kun Xiong, Jinzhong Wang, Tao Ye, Wenping Dong, Qi Wang, Qimin Chen, Yunqi Geng, Charles Wood, Yi Zeng
Nebraska Center for Virology: Faculty Publications
HIV-1HXB2 5′LTR region, most of BIVR29 gag-pol segment and HIV-1HXB2 pol IN-3′LTR region were respectively amplified. A chimeric clone, designated as pHBIV3753, was constructed by cloning three fragments sequentially into pUC18. MT4 cells were transfected with pHBIV3753. The replication and expressions of the chimeric virus (HBIV3753) were monitored by RT activity and IFA. The results firstly demonstrated that it is possible to generate a new type of the BIV/HIV-1 chimeric virus containing BIV gag-pol gene.
Vertical Transmission Of Kaposi's Sarcoma-Associated Herpesvirus, Hamakwa Mantina, Chipepo Kankasa, Winslow Klaskala, Brad Brayfield, James Campbell, Quijiang Du, Ganapati Bhat, Francis Kasolo, Charles Mitchell, Charles Wood
Vertical Transmission Of Kaposi's Sarcoma-Associated Herpesvirus, Hamakwa Mantina, Chipepo Kankasa, Winslow Klaskala, Brad Brayfield, James Campbell, Quijiang Du, Ganapati Bhat, Francis Kasolo, Charles Mitchell, Charles Wood
Nebraska Center for Virology: Faculty Publications
Little is presently known about the specific routes of transmission of Kaposi’s sarcoma-associated herpesvirus (KSHV) or human herpesvirus-8 (HHV-8). To investigate whether this agent might be transmitted vertically from mother to infant, we conducted a study on 89 KSHV seropositive mothers and their newborn infants. Thirteen mothers (14.6%) had KSHV DNA detected in their peripheral blood mononuclear cells (PBMC). Two of 89 samples drawn at birth from infants born to KSHV seropositive mothers had KSHV DNA detectable within their PBMC. These findings suggest that KSHV can be transmitted perinatally, but infrequently. Other routes of transmission such as horizontal transmission remain …
Quantitative Image Analysis Of Simian Immunodeficiency Virus Replication In Macrophages Coinfected With Mycobacterium Avium Complex, Qingsheng Li, Keith G. Mansfield, Andrew Lackner, Ashley T. Haase
Quantitative Image Analysis Of Simian Immunodeficiency Virus Replication In Macrophages Coinfected With Mycobacterium Avium Complex, Qingsheng Li, Keith G. Mansfield, Andrew Lackner, Ashley T. Haase
Qingsheng Li Publications
Mycobacterium avium is the most frequent cause of disseminated bacterial infection in patients with human immunodeficiency virus type 1 infection and in rhesus macaques with simian immunodeficiency virus (SIV) infection. This animal model of AIDS was used to test the hypothesis that this frequent association is the result of reciprocal enhancement of replication of both microorganisms. The replication of M. avium and SIV was analyzed in lymphatic tissues obtained from rhesus macaques experimentally inoculated with SIVmac who developed or remained free of overt M. avium infection. In situ hybridization, quantitative image analysis, and staining of M. avium and of macrophages …
A Study On The Effects Of The N-Terminal Amino Acid Sequence On The Activation Of Human T-Cell Leukemia Virus Type 1 Protease, Hidayah Muhammad Kendall
A Study On The Effects Of The N-Terminal Amino Acid Sequence On The Activation Of Human T-Cell Leukemia Virus Type 1 Protease, Hidayah Muhammad Kendall
Chemistry & Biochemistry Theses & Dissertations
Human T-cell leukemia virus type 1 (HTL V-1) is dependent upon the enzymatic activity of its protease for maturation. Maturation of the protease is facilitated by cleavage of specific amino acid residues, followed by dimerization. The effects of the amino acid sequence located N-terminally to the cleavage site on the ability of the protease to become active were the focus of the current study. These amino acid sequences were contributed by the plasmid vector into which the protease gene was inserted.
Surface probability analyses (SPAs) of the vectors, as well as for native sequences which produce the mature protease and …
Transactivation Of The Moloney Murine Leukemia Virus And T-Cell Receptor Beta-Chain Enhancers By Cbf And Ets Requires Intact Binding Sites For Both Proteins., Wanwen Sun, Barbara J. Graves, Nancy A. Speck
Transactivation Of The Moloney Murine Leukemia Virus And T-Cell Receptor Beta-Chain Enhancers By Cbf And Ets Requires Intact Binding Sites For Both Proteins., Wanwen Sun, Barbara J. Graves, Nancy A. Speck
Dartmouth Scholarship
The Moloney murine leukemia virus (Mo-MLV) enhancer contains binding sites (LVb and LVc) for the ets gene family of proteins and a core site that binds the polyomavirus enhancer-binding protein 2/core-binding factor (cbf) family of proteins. The LVb and core sites in the Mo-MLV enhancer contribute to its constitutive activity in T cells. All three binding sites (LVb, LVc, and core) are required for phorbol ester inducibility of the Mo-MLV enhancer. Adjacent binding sites for the ets and cbf proteins likewise constitute a phorbol ester response element within the human T-cell receptor beta-chain (TCR beta) enhancer and contribute to constitutive …
Structural Characterization Of A Novel Inhibitor Of Hiv Reverse Transcriptase (Hiv Rt), Greggory Jon Woitte
Structural Characterization Of A Novel Inhibitor Of Hiv Reverse Transcriptase (Hiv Rt), Greggory Jon Woitte
Chemistry & Biochemistry Theses & Dissertations
Human immunodeficiency virus (HIV) infections have become a leading cause of death among young people in the United States today. As the number of HIV infections increases, so too does the cost of treatment. Together, these numbers have prompted an increase in the development of pharmaceutical interventions. HIV reverse transcriptase (HIV RT) has become a suitable target for drug therapy because it is the sole enzyme responsible for HIV replication.
Fucoidan, a sulfated polysaccharide isolated from the brown algae Fucus vesiculosus, has been shown to block a variety of cell adhesion related events including metastasis. In addition, fucoidan has also …
The Kinetic Characterization Of Mutant L289p Of Recombinant Hiv-1 Reverse Transcriptase, Tryn Thomas Stimart
The Kinetic Characterization Of Mutant L289p Of Recombinant Hiv-1 Reverse Transcriptase, Tryn Thomas Stimart
Chemistry & Biochemistry Theses & Dissertations
The Human Immunodeficiency Virus, type 1 (HIV-1) is the causative agent of the Acquired immunodeficiency Syndrome (AIDS). Currently, AIDS is the leading cause of death amongst young people in the United States. The large increase of HIV-1 infections over the past decade has spawned considerable research in fighting the spread of this disease. One area of intense HIV-1 research has focused on the enzyme Reverse Transcriptase (RT) which is an essential enzyme involved in the replication and life cycle of HIV-1. HIV-1 RT is composed of p66 and p51 subunits and is only active as a heterodimer in vivo. …
Characterization Of An Antiviral Agent Based On Nonionic Surfactants And It's Effects On Human Dermal Fibroblasts, Ji Young Li
Characterization Of An Antiviral Agent Based On Nonionic Surfactants And It's Effects On Human Dermal Fibroblasts, Ji Young Li
Biological Sciences Theses & Dissertations
The AIDS ·pandemic has directed various research endeavors towards finding an appropriate method for eliminating all potentially infectious material within bone allografts before implantation into a recipient. To that effect, Panavirocide was conceived by Medicine and Applied Science·s, Inc. This compound, in which three nonionic surfactants serve as the active agents, has been shown to inactivate HIV-1 particles within blood and blood products. Because Panavirocide has never been used with allografts, the purpose of the present research was to characterize the nonionic surfactants for the putative alteration of the formulation by determining the critical micelle concentration values and to determine …
Kinetic Characterization Of A Recombinant C-Terminal Mutant Of Reverse Transcriptase From The Human Immunodeficiency Virus, Thomas S. Heard
Kinetic Characterization Of A Recombinant C-Terminal Mutant Of Reverse Transcriptase From The Human Immunodeficiency Virus, Thomas S. Heard
Chemistry & Biochemistry Theses & Dissertations
The human immunodeficiency virus (HIV) reverse transcriptase (RT) (EC 2.7.7.49) is the central replication enzyme for HIV. In general, the kinetic mechanism for this and all other polymerases involves the ordered binding of two substrates: a primer-template (PT) followed by a deoxyribonucleoside triphosphate (dNTP). Previous investigations prompted this research when it was discovered that the substrate dNTP, in absence of PT, could protect a recombinant c-terminal mutant HIV-1 RT from inhibition by pyridoxal-5'-monophosphate (PLP), an active-site dNTP inhibitor. In contrast, the non-mutant recombinant HIV-1 RT required both substrates for protection from PLP inhibition. This investigation sought to determine if this …