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Articles 1 - 5 of 5
Full-Text Articles in Molecular Genetics
Using Fluorescent Microscopy To Follow Mitochondrial Inheritance Through Tagged Alternative Oxidase In Sporisorium Reilianum., Luke Schroeder
Using Fluorescent Microscopy To Follow Mitochondrial Inheritance Through Tagged Alternative Oxidase In Sporisorium Reilianum., Luke Schroeder
College of Arts & Sciences Senior Honors Theses
Sporisorium reilianum is a dimorphic fungus that inhabits and infects a host corn plant (Zea mays). In order for the fungus to reproduce sexually, compatible haploid mating types must form a dikaryon that goes on to cause infection in the host. This infection causes leaf chlorosis and gall formation, while ultimately allowing for the dispersal of fungal teliospores in the later stages of infection. To grow, the fungus requires energy production in the form of ATP from its mitochondria. As a countermeasure to infection, host plants release harsh reactive oxygen species that may damage DNA, lead to apoptosis, …
Functional Characterization Of A Putative Alternative Oxidase In Sporisorium Reilianum F. Sp. Zeae., Emma A Lamb
Functional Characterization Of A Putative Alternative Oxidase In Sporisorium Reilianum F. Sp. Zeae., Emma A Lamb
College of Arts & Sciences Senior Honors Theses
Sporisorium reilianum is a pathogenic basidiomycete fungus with two formae speciales, each capable of infecting corn (SRZ) or sorghum (SRS), respectively. This fungus is also a dimorphic variety, meaning it can switch between its haploid, yeast-like sporidia and diploid teliospore stages over the course of its life cycle (Schirawski). When S. reilianum is found in a haploid state and conditions are favorable, it will mate with a compatible non-self mating type to begin filamentous growth and proliferation in the plant host (Zhao). S. relianum, like most fungi, utilizes the four classical components of the electron transport chain to produce …
Implementing Crispr-Cas9 Gene Editing System In Microbotryum., Stevana Reese Schauer
Implementing Crispr-Cas9 Gene Editing System In Microbotryum., Stevana Reese Schauer
College of Arts & Sciences Senior Honors Theses
Microbotryum dianthorum is a species of smut fungus which causes infection in plants in the Dianthus genus. The infection process is aided by small-secreted proteins known as effectors. Effectors may aid in the infection process because they are secreted by the fungus and can enter plant tissue, possibly to facilitate infection and/or manipulate the host. Thus, it is of interest to investigate the genes for these effectors to determine their role in the infection process. One method to study genes is through gene disruption, via the CRISPR/Cas9 gene editing system, but this has not yet been implemented in Microbotryum dianthorum …
Genetic Crosstalk And Its Effects On Virulence And Mating In Ustilago Maydis., John S. Desmarais
Genetic Crosstalk And Its Effects On Virulence And Mating In Ustilago Maydis., John S. Desmarais
College of Arts & Sciences Senior Honors Theses
Ustilago maydis is a model organism for study of fungal mating and host infection. Two compatible haploid mating types must mate to form a dikaryon in order for the fungus to infect its host, corn (Zea mays). There are a variety of genetic mechanisms that regulate mating and infection in the fungus, many of which coregulate both processes. The aim of this study was to investigate how alteration of certain regulatory proteins in U. maydis affects these basic processes as well as how alteration of genes involved in signaling pathways can affect the expression of one another. Primarily, …
The Role Of Ferric Reduction Oxidases In Plant Anti-Herbivore Defense., Virginia C. Nunamaker
The Role Of Ferric Reduction Oxidases In Plant Anti-Herbivore Defense., Virginia C. Nunamaker
College of Arts & Sciences Senior Honors Theses
Iron is an essential element required for plants to carry out metabolic functions such as photosynthesis, heme biosynthesis, and chlorophyll biosynthesis. Within Arabidopsis thaliana, eight ferric reduction oxidase (FRO) genes function in iron uptake and homeostasis with tissue specific expression. However, little else is known regarding the biological role of FROs. Recent studies identify the FRO gene family as particularly responsive to the green leaf plant derived volatile (GLV) cis-3-hexenyl acetate (z3HAC). Since z3HAC acts as a wound signal and cues unaffected parts of the plant to prime defenses prior to herbivory, an increase in FRO activity in response …