Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Biology (6)
- Bioinformatics (4)
- Computational Biology (3)
- Animal Sciences (2)
- Biotechnology (2)
-
- Entomology (2)
- Genetics (2)
- Molecular Genetics (2)
- Nutrition (2)
- Plant Sciences (2)
- Agriculture (1)
- Aquaculture and Fisheries (1)
- Art and Design (1)
- Arts and Humanities (1)
- Biochemistry, Biophysics, and Structural Biology (1)
- Biosecurity (1)
- Botany (1)
- Cell and Developmental Biology (1)
- Fiber, Textile, and Weaving Arts (1)
- Immunology and Infectious Disease (1)
- Microbiology (1)
- Other Cell and Developmental Biology (1)
- Parasitology (1)
- Plant Biology (1)
- Sheep and Goat Science (1)
- Institution
- Keyword
-
- Insect Systematics (2)
- Methods (2)
- Sequence Alignment and Phylogenetics (2)
- <p>Dusky salamanders.</p> <p> Salamanders -- Appalachian Region, Southern.</p> (1)
- Animal genetics and genomics (1)
-
- Animals (1)
- Artificial insemination (1)
- Axons (1)
- Bioinformatics (1)
- Biological (1)
- Caenorhabditis elegans (1)
- Cell differentiation (1)
- Cell movement (1)
- Cell separation (1)
- Chloroplasts (1)
- Crop stubble (1)
- Cysteine (1)
- Databases (1)
- Drench resistance (1)
- Enzymology (1)
- Ephemeroptera; MayXies; Molecular phylogeny; Evolution of Xight; Origin of wings; Pterygota; Mandibular tusks; Sensitivity analysis; Direct optimization (1)
- Evolutionary biology (1)
- Ewe trial (1)
- Feedlot (1)
- Flow cytometry (1)
- Fluorescence (1)
- Gene expression profiling (1)
- Gene expression regulation (1)
- Genes (1)
- Genetic (1)
- Publication
- Publication Type
- File Type
Articles 1 - 12 of 12
Full-Text Articles in Genomics
Efficient Gene-Driven Germ-Line Point Mutagenesis Of C57bl/6j Mice, Edward J. Michaud, Cymbeline T. Culiat, Mitchell L. Klebig, Paul E. Barker, Kt Cain, Debra J. Carpenter, Lori L. Easter, Carmen M. Foster, Alysyn W. Gardner, Zy Guo, Kay J. Houser, Lori A. Hughes, Marilyn K. Kerley, Zhaowei Liu, Robert E. Olszewski, Irina Pinn, Ginger D. Shaw, Sarah G. Shinpock, Ann M. Wymore, Eugene M. Rinchik, Dabney K. Johnson
Efficient Gene-Driven Germ-Line Point Mutagenesis Of C57bl/6j Mice, Edward J. Michaud, Cymbeline T. Culiat, Mitchell L. Klebig, Paul E. Barker, Kt Cain, Debra J. Carpenter, Lori L. Easter, Carmen M. Foster, Alysyn W. Gardner, Zy Guo, Kay J. Houser, Lori A. Hughes, Marilyn K. Kerley, Zhaowei Liu, Robert E. Olszewski, Irina Pinn, Ginger D. Shaw, Sarah G. Shinpock, Ann M. Wymore, Eugene M. Rinchik, Dabney K. Johnson
Faculty Publications and Other Works -- General Biology
Background
Analysis of an allelic series of point mutations in a gene, generated by N-ethyl-N-nitrosourea (ENU) mutagenesis, is a valuable method for discovering the full scope of its biological function. Here we present an efficient gene-driven approach for identifying ENU-induced point mutations in any gene in C57BL/6J mice. The advantage of such an approach is that it allows one to select any gene of interest in the mouse genome and to go directly from DNA sequence to mutant mice.
Results
We produced the Cryopreserved Mutant Mouse Bank (CMMB), which is an archive of DNA, cDNA, tissues, and …
Characterization Of The Yeast Ionome: A Genome-Wide Analysis Of Nutrient Mineral And Trace Element Homeostasis In Saccharomyces Cerevisiae, David J. Eide, Suzanne Clark, T . Murlidharan Nair, Mathias Gehl, Michael Gribskov, Mary Lou Guerinot, Jeffrey Harper
Characterization Of The Yeast Ionome: A Genome-Wide Analysis Of Nutrient Mineral And Trace Element Homeostasis In Saccharomyces Cerevisiae, David J. Eide, Suzanne Clark, T . Murlidharan Nair, Mathias Gehl, Michael Gribskov, Mary Lou Guerinot, Jeffrey Harper
Dartmouth Scholarship
Nutrient minerals are essential yet potentially toxic, and homeostatic mechanisms are required to regulate their intracellular levels. We describe here a genome-wide screen for genes involved in the homeostasis of minerals in Saccharomyces cerevisiae. Using inductively coupled plasma-atomic emission spectroscopy (ICP-AES), we assayed 4,385 mutant strains for the accumulation of 13 elements (calcium, cobalt, copper, iron, potassium, magnesium, manganese, nickel, phosphorus, selenium, sodium, sulfur, and zinc). We refer to the resulting accumulation profile as the yeast 'ionome'.
Sheep Updates 2005 - Part 7, S. M. Liu, A. C. Schlink, J. R. Williams, M. E. Dowling, J. C. Greeff, Roy Butler, Keith Croker, L. G. Butler, Rob Davidson, Ken Hart, Doug Harrington, Mario D'Antuono, Julian Gardner, Geoff Duddy, R. G. Woodgate, A. Le Feuvre, A Bailey, R. B. Besier, N. Campbell, I. Carmichael, S. Love, Richard Stovold
Sheep Updates 2005 - Part 7, S. M. Liu, A. C. Schlink, J. R. Williams, M. E. Dowling, J. C. Greeff, Roy Butler, Keith Croker, L. G. Butler, Rob Davidson, Ken Hart, Doug Harrington, Mario D'Antuono, Julian Gardner, Geoff Duddy, R. G. Woodgate, A. Le Feuvre, A Bailey, R. B. Besier, N. Campbell, I. Carmichael, S. Love, Richard Stovold
Sheep Updates
This session covers eight papers from different authors:
POSTERS
1. Sulpher in wool and its implications for fleece weight and sheep health, SM Liu, AC Schlink, JR Williams, CSIRO Livestock Industries Wembley WA, ME Dowling,JCGreef, Department of Agriculture Western Australia.
2. Stubbles for sheep: a reality check, Roy Butler, Keith Croker, Department of Agriculture Western Australia.
3. Genetic benchmarking using artificial insemination, LC Butler, JC Greeff, Department of Agriculture Western Australia.
4. The potential lambing performances of ewes in mixed age flocks, Kieth Croker, Department of Agriculture Western Australia, Rob Davidson, WAMMCO International, formally University of Western Australia, Ken Hart, …
Methods For Obtaining And Analyzing Whole Chloroplast Genome Sequences, Robert K. Jansen, Linda A. Raubeson, Jeffrey L. Boore, Claude W. Depamphilis, Timothy W. Chumley, Rosemarie C. Haberle, Stacia K. Wyman, Andrew J. Alverson, Riannon Peery, Sallie J. Herman, H. Matthew Fourcade, Jennifer V. Kuehl, Joel R. Mcneal, James Leebens-Mack, Liying Cui
Methods For Obtaining And Analyzing Whole Chloroplast Genome Sequences, Robert K. Jansen, Linda A. Raubeson, Jeffrey L. Boore, Claude W. Depamphilis, Timothy W. Chumley, Rosemarie C. Haberle, Stacia K. Wyman, Andrew J. Alverson, Riannon Peery, Sallie J. Herman, H. Matthew Fourcade, Jennifer V. Kuehl, Joel R. Mcneal, James Leebens-Mack, Liying Cui
All Faculty Scholarship for the College of the Sciences
During the past decade there has been a rapid increase in our understanding of plastid genome organization and evolution due to the availability of many new completely sequenced genomes. Currently there are 43 complete genomes published and ongoing projects are likely to increase this sampling to nearly 200 genomes during the next five years. Several groups of researchers including ours have been developing new techniques for gathering and analyzing entire plastid genome sequences and details of these developments are summarized in this chapter. The most important recent developments that enhance our ability to generate whole chloroplast genome sequences involve the …
A Gene Expression Fingerprint Of C. Elegans Embryonic Motor Neurons, Rebecca M. Fox, Stephen E. Von Stetina, Susan J. Barlow, Christian Shaffer, Kellen L. Olszewski, Jason H. Moore
A Gene Expression Fingerprint Of C. Elegans Embryonic Motor Neurons, Rebecca M. Fox, Stephen E. Von Stetina, Susan J. Barlow, Christian Shaffer, Kellen L. Olszewski, Jason H. Moore
Dartmouth Scholarship
Differential gene expression specifies the highly diverse cell types that constitute the nervous system. With its sequenced genome and simple, well-defined neuroanatomy, the nematode C. elegans is a useful model system in which to correlate gene expression with neuron identity. The UNC-4 transcription factor is expressed in thirteen embryonic motor neurons where it specifies axonal morphology and synaptic function. These cells can be marked with an unc-4::GFP reporter transgene. Here we describe a powerful strategy, Micro-Array Profiling of C. elegans cells (MAPCeL), and confirm that this approach provides a comprehensive gene expression profile of unc-4::GFP motor neurons in vivo.
.
Vernalization Sensitivity In Arabidopsis Thaliana (Brassicaceae): The Effects Of Latitude And Flc Variation, Ana Lucia Caicedo, M. D. Purugganan, C. Mays, R. Hopkins, J. R. Stinchcombe, J. Schmitt, J. R. Stichcombe
Vernalization Sensitivity In Arabidopsis Thaliana (Brassicaceae): The Effects Of Latitude And Flc Variation, Ana Lucia Caicedo, M. D. Purugganan, C. Mays, R. Hopkins, J. R. Stinchcombe, J. Schmitt, J. R. Stichcombe
Ana Lucia Caicedo
Latitudinal variation in climate is predicted to select for latitudinal differentiation in sensitivity to the environmental cues that signal plants to flower at the appropriate time for a given climate. In Arabidopsis thaliana, flowering is promoted by exposure to cold temperatures (vernalization), and several vernalization pathway loci are known. To test whether natural variation in vernalization sensitivity could account for a previously observed latitudinal cline in flowering time in A. thaliana, we exposed 21 European accessions to 0, 10, 20, or 30 d of vernalization and observed leaf number at flowering under short days in a growth chamber. We observed …
Comparative Plant Genomics. Frontiers And Prospects, Ana Lucia Caicedo, M. D. Purugganan
Comparative Plant Genomics. Frontiers And Prospects, Ana Lucia Caicedo, M. D. Purugganan
Ana Lucia Caicedo
No abstract provided.
Analysis Of Genotypic And Phenotypic Differences In Desmognathus Quadramaculatus Across The Southern Appalachians, Deborah Susan Merritt
Analysis Of Genotypic And Phenotypic Differences In Desmognathus Quadramaculatus Across The Southern Appalachians, Deborah Susan Merritt
Theses, Dissertations and Capstones
Populations of Desmognathus quadramaculatus were compared for genotypic and phenotypic differences across 5 states in the Southern Appalachians. Series consisting of 10 salamanders each were collected from West Virginia, Virginia, Tennessee, North Carolina and Georgia. Salamanders were assessed for genotypic differences by using allozyme (proteins with polymorphic loci) electrophoresis staining for 13 loci of the muscle, liver and stomach tissue. Nei’s genetic distance was used to score genetic variation between populations. Phenotypic differences were assessed by comparing 10 external morphological measurements between populations. Mahalanobis’ distance was used to score morphological differences between populations. Both genetic and morphological differences were correlated …
Bioinformatic Analysis Of The Metal-Binding Protein Families And Heavy Metal Resistance Amongst Cyanobacteria, Tin-Chun Chu, Lee Lee, Shankar Srinivasan
Bioinformatic Analysis Of The Metal-Binding Protein Families And Heavy Metal Resistance Amongst Cyanobacteria, Tin-Chun Chu, Lee Lee, Shankar Srinivasan
Tin-Chun Chu, Ph.D.
No abstract provided.
Plant Ontology (Po): A Controlled Vocabulary Of Plant Structures And Growth Stages, Pankaj Jaiswal, Shulamit Avraham, Katica Ilic, Elizabeth A. Kellogg, Susan Mccouch, Anuradha Pujar, Leonore Reiser, Seung Y. Rhee, Martin M. Sachs, Mary L. Schaeffer, Lincoln Stein, Peter Stevens, Leszek Vincent, Doreen Ware, Felipe Zapata
Plant Ontology (Po): A Controlled Vocabulary Of Plant Structures And Growth Stages, Pankaj Jaiswal, Shulamit Avraham, Katica Ilic, Elizabeth A. Kellogg, Susan Mccouch, Anuradha Pujar, Leonore Reiser, Seung Y. Rhee, Martin M. Sachs, Mary L. Schaeffer, Lincoln Stein, Peter Stevens, Leszek Vincent, Doreen Ware, Felipe Zapata
Peter Stevens
Poor Taxon Sampling, Poor Character Sampling, And Non-Repeatable Analyses Of A Contrived Dataset Do Not Provide A More Credible Estimate Of Insect Phylogeny: A Reply To Kjer., T. Heath Ogden
T. Heath Ogden
The wealth of data available for phylogenetic analysis of the insect orders, from both morphological and molecular sources, is steadily increasing. However, controversy exists among the methodologies one can use to reconstruct ordinal relationships. Recently, Kjer (2004) presented an analysis of insect ordinal relationships based exclusively on a single source of information: 18S rDNA sequence data. Kjer claims that his analysis resulted in a more ‘‘credible’’ phylogeny for the insect orders and strongly criticized our previous phylogenetic results. However, Kjer only used a subset of the data that are currently available for insect ordinal phylogeny, misrepresented our analyses, and omitted …
Phylogeny Of Ephemeroptera (Mayflies) Based On Molecular Evidence, T. Heath Ogden
Phylogeny Of Ephemeroptera (Mayflies) Based On Molecular Evidence, T. Heath Ogden
T. Heath Ogden
This study represents the Wrst molecular phylogeny for the Order Ephemeroptera. The analyses included 31 of the 37 families, representing »24% of the genera. Fifteen families were supported as being monophyletic, Wve families were supported as nonmonophyletic, and 11 families were only represented by one species, and monophyly was not testable. The suborders Furcatergalia and Carapacea were supported as monophyletic while Setisura and Pisciforma were not supported as monophyletic. The superfamilies Ephemerelloidea and Caenoidea were supported as monophyletic while Baetoidea, Siphlonuroidea, Ephemeroidea, and Heptagenioidea were not. Baetidae was recovered as sister to the remaining clades. The mayXy gill to wing …