Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Genomics
Characterization Of Chemical And Radiation-Induced Dna Damage In The Mdbk Cell Culture System, Jeffrey R. Hincks
Characterization Of Chemical And Radiation-Induced Dna Damage In The Mdbk Cell Culture System, Jeffrey R. Hincks
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
A mechanism common to many carcinogens and mutagens is the interaction with cellular DNA. Alkaline elution is a sensitive and commonly used technique to detect cellular DNA damage in the form of DNA single-strand breaks (SSB), DNA-protein cross-links (DPC), and DNA interstrand cross-links (ISC). In the present study, gravity-flow alkaline elution was developed and validated to rapidly detect DNA damage in the MDBK cell culture system by genotoxic agents.
The system was first validated by detecting a dose dependent induction of DNA SSB in cells exposed to 25-1500 rad of X-ray. The assay reliably detected the DNA damage of seven …