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Articles 1 - 30 of 38
Full-Text Articles in Genomics
The Discovery Of A Novel Bacteria From A Large Co-Assembly Of Metagenomes, Matthew Finkelberg
The Discovery Of A Novel Bacteria From A Large Co-Assembly Of Metagenomes, Matthew Finkelberg
Masters Theses
In the summer of 2022, a co-assembly of metagenome was created using the microbes found at Barres Woods in Harvard Forest. 14 samples were taken, and sample was split into the organic and mineral layer, which totals 28 Bulk MAGs. Within this Co-assembly, 4 different genomes were found which were designated with the phylum of FCPU426. Three of which were considered medium quality and one being assigned high quality. The novel phyla first appeared in NCBI and GTDB databases in June 2018. The name FCPU426 dates to 2010 and was named based on the 16s amplicon sequencing.
The novel phylum …
The Genomic Dynamics Of Fermented Food Microbes, Katherine Chacón-Vargas
The Genomic Dynamics Of Fermented Food Microbes, Katherine Chacón-Vargas
Doctoral Dissertations
Along with the agriculture and domestication revolution, humans have utilized bacteria, yeasts, and molds for millennia in the production of traditionally fermented foods and beverages. Fermentation is a very ancient practice of high relevance nowadays since it contributes with a great variety of foods worldwide. Microbial fermentation allows metabolic transformation of the raw food materials leading to biochemical changes that played a key role in food preservation, health benefits, nutrition, flavors, and texture, among others. Food fermentation practices could diverge from traditional artisanal spontaneous fermentation to industrialize methods with specialized microbial starters and although fermented environments tend to be very …
Changes In Gene Expression From Long-Term Warming Revealed Using Metatranscriptome Mapping To Fac-Sorted Bacteria, Christopher A. Colvin
Changes In Gene Expression From Long-Term Warming Revealed Using Metatranscriptome Mapping To Fac-Sorted Bacteria, Christopher A. Colvin
Masters Theses
Soil microbiomes play pivotal roles to the health of the environment by maintaining metabolic cycles. One question is how will climate change affect soil bacteria over time and what could the repercussions be. To answer these questions, the Harvard Forest Long-Term Warming Experiment was established to mimic predicted climate change by warming plots of land 5℃ above ambient conditions. In 2017, 14 soil core samples were collected from Barre Woods warming experiment to mark 15 years since the establishment of the soil warming in that location. These samples underwent traditional metatranscriptomics to generate an mRNA library as well as a …
Chemosensory Receptors In Berghia Stephanieae: Bioinformatics And Localization, Kelsi L. Watkins
Chemosensory Receptors In Berghia Stephanieae: Bioinformatics And Localization, Kelsi L. Watkins
Masters Theses
Chemosensation is achieved through the binding of chemical signals to chemoreceptor proteins embedded in the membranes of sensory neurons. The molecular identity of these receptors, as well as the downstream processing of chemosensory signals, has been well studied in arthropods and vertebrates. However, very little is known about molluscan chemosensation. The identity of chemoreceptor proteins in the nudibranch mollusc Berghia stephanieae are unknown. Data from other protostome and molluscan studies suggest Berghia may use ionotropic receptors for some forms of chemoreception. This study used a bioinformatics approach to identify potential chemosensory ionotropic receptors in the transcriptome of Berghia. A …
Screening For Binding Partners And Protein-Protein Interactions Of A Fungal Transcription Factor- Xdr1, Nishadi Punsara Gallala Gamage
Screening For Binding Partners And Protein-Protein Interactions Of A Fungal Transcription Factor- Xdr1, Nishadi Punsara Gallala Gamage
Masters Theses
Clarireedia spp. (formerly Sclerotinia homoeocarpaF.T. Bennett) is the causal agent dollar spot, the most economically important turfgrass disease impacting golf courses in North America. The most effective strategy for dollar spot control is repeated application of multiple classes of fungicides. However, reliance on chemical application has led to resistance to four classes of fungicides as well as multidrug resistance (MDR). Fungi are known to detoxify xenobiotics, like fungicides, through transcriptional regulation of three detoxification phases: modification, conjugation and secretion. Little is known, however, of the protein-protein interactions that facilitate these pathways. Following next-generation RNA sequencing of Clarireedia spp., a …
Genomic Organization And Expression Of The Wc1 Hybrid Coreceptor And Pattern Recognition Receptor On Porcine Gamma Delta T Cells, Lauren Le Page
Genomic Organization And Expression Of The Wc1 Hybrid Coreceptor And Pattern Recognition Receptor On Porcine Gamma Delta T Cells, Lauren Le Page
Doctoral Dissertations
gd T cells are a crucial component of the immune response to a number of increasingly relevant and largely zoonotic pathogens to which efficacious vaccination is lacking. In ruminants and swine, gd T cells represent a major population of peripheral blood and epithelial tissue-resident lymphocytes. gdT cells respond to both protein and non-protein antigens independently of MHC presentation and possess immunological memory. Upon activation, gamma delta T cells illicit a variety of effector functions and play an indispensable role of orchestrating the downstream immune response. These characteristics make gamma delta T cells a promising candidate for recruitment by vaccination, however, …
Genetic And Environmental Regulation Of Plant Growth, Kirk J-M Mackinnon
Genetic And Environmental Regulation Of Plant Growth, Kirk J-M Mackinnon
Doctoral Dissertations
Field grown crops are continually exposed to a variety of external stimuli that influence plant responses. Light, temperature, and water availability interact to affect many economically important traits including growth rate, size, and lifespan. My research is focused on the intersection of genetic and environmental factors influencing plant growth. Specifically, I am interested in elucidating the regulation of rhythmic genes in response to photo- and thermocycles and identifying novel candidate genes associated with growth and drought traits. Understanding the gene regulatory networks that mediate time-of-day signaling is vital to identifying candidate genes across the pan-genome associated with traits of interest.
Mapping Selected Polyphenols Metabolism By Gut Bacteria And Their Genes, Ermin Zhao
Mapping Selected Polyphenols Metabolism By Gut Bacteria And Their Genes, Ermin Zhao
Doctoral Dissertations
The human gut microbiome is a huge enzyme repository for dietary polyphenols metabolism, especially considering most of the polyphenols cannot be digested in the host and their biological functions are limited. Poor bioaccessibility based on traditional pharmaceutical ADME (absorption, distribution, metabolism, and excretion) assessment is the main problem facing the widely medical application of most polyphenols. Gut bacteria have the potential to mediate a wide range of biotransformation reactions of polyphenols, which leads to the production of many bioactive metabolites. In the past decades, mounting evidence in traditional ADME study have demonstrated gut bacteria play an irreplaceable role in dietary …
Discovering Mechanisms Driving Adaptive Evolution In The Cross-Kingdom Fungal Pathogen Fusarium Oxysporum, Dilay Hazal Ayhan
Discovering Mechanisms Driving Adaptive Evolution In The Cross-Kingdom Fungal Pathogen Fusarium Oxysporum, Dilay Hazal Ayhan
Doctoral Dissertations
Fusarium oxysporum is a cross-kingdom pathogenic fungus that can cause vascular wilt disease in many economically important plants and local or disseminated infections in humans. Although it lacks a sexual stage in its life cycle, F. oxysporum can adapt to a wide range of hosts because of accessory chromosomes (ACs) which are enriched in host-specific genes and repeat content. This dissertation investigates the mechanisms that drive the adaptive evolution in the cross-kingdom pathogen F. oxysporum using comparative genomics and an experimental evolution approach. The first chapter compares phenotypes and genomes of a plant pathogenic isolate F. oxysporum f. sp. lycopersici …
Effects Of Tempeh Fermentation On Soy Free And Bound Phenolics: Release, Transformation, And Stimulated Production, Amadeus Driando Ahnan
Effects Of Tempeh Fermentation On Soy Free And Bound Phenolics: Release, Transformation, And Stimulated Production, Amadeus Driando Ahnan
Doctoral Dissertations
The fermentation of tempeh, a traditional source of protein originated in Indonesia, has been reported to enhance the health-promoting potentials of various grains, legumes, and beans. Tempeh fermentation on soybeans can modulate the bioavailability of phenolic compounds, particularly isoflavones, bioactive compounds that have been found to be protective against lung, prostrate, and colon cancers. However, the mechanism of the protective benefits was unknown. Using whole-food and in vitro models, this study addressed this research gap by investigating the effects of tempeh fermentation using various cultures on the compositions of soy free, bound, and minor phenolics, as well as their antioxidative, …
Accessory Genes Contribute To Rewiring The Transcriptional Network In Fusarium Oxysporum, He Yang
Accessory Genes Contribute To Rewiring The Transcriptional Network In Fusarium Oxysporum, He Yang
Doctoral Dissertations
The presence of accessory chromosomes is one of the most fascinating features in the genomes of Fusarium oxysporum (Fo) species complex that includes both plant and animal pathogens. In plant-pathogenic Fo strains, these accessory chromosomes govern host-specific pathogenicity through unique sets of virulence factors, including secreted effectors and plant cell wall degrading enzymes, while the identity of such factors are largely unknown in human infecting strains. This dissertation is composed of three projects that focused on studying the gene composition and transcriptional regulation of human-pathogenic Fo strains. The first project deciphered the genome of the clinical Fo strain, …
Characterizing Adaptive Non-Coding Changes In The Regulation Of Human Gene Expression, Jason Pizzollo
Characterizing Adaptive Non-Coding Changes In The Regulation Of Human Gene Expression, Jason Pizzollo
Doctoral Dissertations
Differential patterns of gene expression contribute to phenotypic differences between species. Understanding evolutionary changes in gene regulatory elements can help explain traits that separate humans from closely related species. Here, in two separate studies, we investigate gene expression and gene regulatory differences between humans our closest living evolutionary relatives, chimpanzees, in the context of uniquely human traits: increased susceptibility to epithelial cancers and neural developmental and functional processes that underlie our increased cognitive capacity. Using genomic methods to study gene expression and open chromatin, we compare human and chimpanzee responses to a serum challenge, an assay that that mimics patterns …
De-Coding The Impact Of Evolved Changes In Gene Expression And Cellular Phenotype On Primate Evolution, Trisha Zintel
De-Coding The Impact Of Evolved Changes In Gene Expression And Cellular Phenotype On Primate Evolution, Trisha Zintel
Doctoral Dissertations
The goal of the dissertation work outlined here was to investigate the influence of proximal processes contributing to evolutionary differences in phenotypes among primate species. There are numerous previous comparative analyses of gene expression between primate brain regions. However, primate brain tissue samples are relatively rare, and my results have contributed to the pre-existing data on more well-studied primates (i.e. humans, chimpanzees, macaques, marmosets) as well as produced information on more rarely-studied primates (i.e. patas monkey, siamang, spider monkey). Additionally, the primary visual cortex has not previously been as extensively studied at the level of gene expression as other brain …
Drivers And Consequences Of Carbon Use Efficiency - And Its Measurement In Soil, Grace Pold
Drivers And Consequences Of Carbon Use Efficiency - And Its Measurement In Soil, Grace Pold
Doctoral Dissertations
Soils serve as massive carbon sinks, but their ability to continue this ecological service is contingent on how the resident soil microbial community will respond to the ongoing climate crisis. One key dimension of the microbial response to warming is its carbon use efficiency (CUE), or the fraction of carbon taken up by an organism which is allocated to growth rather than respiration. However, the scientific community is still in the early stages of understanding the drivers, consequences - and even accurate measurements of - CUE. In this dissertation, I first quantified the variability of CUE and its responsiveness to …
A Plant Pathology View Of Signaling: A Computational Study Of Fusarium Oxysporum Kinomes And Downy Mildew Resistance In Sweet Basil, Gregory Deiulio
A Plant Pathology View Of Signaling: A Computational Study Of Fusarium Oxysporum Kinomes And Downy Mildew Resistance In Sweet Basil, Gregory Deiulio
Doctoral Dissertations
This dissertation is composed of two projects that focus on pathogen and plant signaling within the framework of plant pathology. The first project targets protein kinases within the species complex Fusarium oxysporum based on genomic information and tracks their presence/absence and copy number variation across evolutionary time. We have predicted the kinomes of 19 Ascomycete fungi using the kinase annotating software Kinannote. Among Fusaria, kinases related to the perception of the environment, such as Histidine kinases, are proliferated. Similarly, I observed the expansion of Target of Rapamycin (TOR) kinase that regulates cell growth and development in responding to environmental cues. …
Impacts Of Genome And Nuclear Architecture On Molecular Evolution In Eukaryotes, Xyrus Maurer-Alcalá
Impacts Of Genome And Nuclear Architecture On Molecular Evolution In Eukaryotes, Xyrus Maurer-Alcalá
Doctoral Dissertations
The traditional view of genomes suggests that they are static entities changing slowly in sequence and structure through time (e.g. evolving over geological time-scales). This outdated view has been challenged as our understanding of the dynamic nature of genomes has increased. Changes in DNA content (i.e. polyploidy) are common to specific life-cycle stages in a variety of eukaryotes, as are changes in genome content itself. These dramatic genomic changes include chromosomal deletions (i.e. paternal chromosome deletion in insects; Goday and Esteban 2001; Ross, et al. 2010), developmentally regulated genome rearrangements (e.g. the V(D)J system in adaptive immunity in mammals; Schatz …
All Roads Lead To Weediness: Stories About Weedy Rice Origins, Weedy Genes And Weed Competitiveness, Zhongyun Huang
All Roads Lead To Weediness: Stories About Weedy Rice Origins, Weedy Genes And Weed Competitiveness, Zhongyun Huang
Doctoral Dissertations
Weedy rice (Oryza spp.), a weedy relative of cultivated rice (O.sativa), infests and persists in cultivated rice fields worldwide. Many weedy rice populations have evolved similar adaptive traits, considered part of the ‘agricultural weed syndrome’, making this an ideal model to study the genetic basis of parallel evolution. Using population genetics analyses of South Asian and US weedy rice, my research reveals multiple independent evolution events giving rise to weed groups in the two geographic areas. Weeds in South Asia have highly heterogenous genetic backgrounds, with contributions from both cultivated varieties (aus and indica) …
Expansion Of And Reclassification Within The Family Lachnospiraceae, Kelly N. Haas
Expansion Of And Reclassification Within The Family Lachnospiraceae, Kelly N. Haas
Doctoral Dissertations
Many of the taxa in the family Lachnospiraceae are currently misclassified as Clostridium spp. Here attempt to rectify many of these issues, beginning with an in-depth genomic and physiologic analysis of Clostridium methoxybenzovorans, culminating in the assertion that is a heterotype of Clostridium indolis, followed by reclassification of the broader group in which this organism resides. We propose two novel genera, Lacriformis and Enterocloster, to reclassify this clade, this includes reclassification of Clostridium sphenoides, Clostridium indolis, Clostridium saccharolyticum, Clostridium celerecrescens, Clostridium xylanolyticum, Clostridium algidixylanolyticum, Clostridium aerotolerans, Clostridium amygdalinum, and …
Novel Advancements For Improving Sprout Safety, Kyle S. Landry
Novel Advancements For Improving Sprout Safety, Kyle S. Landry
Doctoral Dissertations
All varieties of bean sprouts (mung bean, alfalfa, broccoli, and radish) are classified as a “super-food” and are common staples for health conscious consumers. Along with the proposed health benefits, there is also an inherent risk of foodborne illness. When sprouts are cooked, there is little risk of illness. The purpose of this dissertation was to explore novel techniques to minimize or prevent the incidence of foodborne illness associated with the consumption of sprouts. Three areas were investigated: 1) the use of a biocontrol organism, 2) the use of a novel spontaneous carvacrol nanoemulsion, and 3) the influence of the …
Gemini: A Computationally-Efficient Search Engine For Large Gene Expression Datasets, Timothy Defreitas, Hachem Saddiki, Patrick Flaherty
Gemini: A Computationally-Efficient Search Engine For Large Gene Expression Datasets, Timothy Defreitas, Hachem Saddiki, Patrick Flaherty
Mathematics and Statistics Department Faculty Publication Series
Background
Low-cost DNA sequencing allows organizations to accumulate massive amounts of genomic data and use that data to answer a diverse range of research questions. Presently, users must search for relevant genomic data using a keyword, accession number of meta-data tag. However, in this search paradigm the form of the query – a text-based string – is mismatched with the form of the target – a genomic profile.
Results
To improve access to massive genomic data resources, we have developed a fast search engine, GEMINI, that uses a genomic profile as a query to search for similar genomic profiles. GEMINI …
The Role Of Bacterial Microcompartments In The Fermentation Of D-Arabinose In Clostridium Phytofermentans, Megan A. Strough
The Role Of Bacterial Microcompartments In The Fermentation Of D-Arabinose In Clostridium Phytofermentans, Megan A. Strough
Masters Theses 1911 - February 2014
Bacterial microcompartments, or BMCs, are 80-200nm, enzyme-encompassing organelles composed of interlocking proteins that form cyclical hexamers with a small central pore. Clostridium phytofermentans or Cphy, is a Gram-positive, rod shaped, anaerobic soil microbe that has the ability to not only break down multiple polysaccharides simultaneously but also proceeds to ferment them into biofuels. The genome of Cphy contains 3 BMC loci. During growth on fucose and rhamnose, one of these loci is highly expressed and microcompartments can be viewed using TEM. Under these growth conditions, three products, ethanol, propanol and propionate, which could potentially be highly useful in the biofuel …
Comparative Genome Analysis Between Agrostis Stolonifera And Members Of The Pooideae Subfamily Including Brachypodium Distachyon, Loreto P. Araneda
Comparative Genome Analysis Between Agrostis Stolonifera And Members Of The Pooideae Subfamily Including Brachypodium Distachyon, Loreto P. Araneda
Masters Theses 1911 - February 2014
Understanding of grass genome structure and evolution has been significantly advanced through comparative genomics. The genomes of most cool-season forage and turf grasses, belonging to the Pooideae subfamily of the grasses, remain understudied. Creeping bentgrass (Agrostis stolonifera) is one of the most important cool-season turfgrasses due to its low mowing tolerance and aggressive growth habit. An RFLP genetic map of creeping bentgrass using 229 RFLP markers derived from cereal and creeping bentgrass EST-RFLP probes was constructed for a comparative genome analysis. This genetic map was compared with those of perennial ryegrass, oat, wheat, and rice. Large-scale chromosomal rearrangements …
Timing Is Everything: Early Degradation Of Abscission Layer Is Associated With Increased Seed Shattering In U.S. Weedy Rice, Ana Caicedo, C. Thurber, P. Helper
Timing Is Everything: Early Degradation Of Abscission Layer Is Associated With Increased Seed Shattering In U.S. Weedy Rice, Ana Caicedo, C. Thurber, P. Helper
Biology Department Faculty Publication Series
Background Seed shattering, or shedding, is an important fitness trait for wild and weedy grasses. U.S. weedy rice (Oryza sativa) is a highly shattering weed, thought to have evolved from non-shattering cultivated ancestors. All U.S. weedy rice individuals examined to date contain a mutation in the sh4 locus associated with loss of shattering during rice domestication. Weedy individuals also share the shattering trait with wild rice, but not the ancestral shattering mutation at sh4; thus, how weedy rice reacquired the shattering phenotype is unknown. To establish the morphological basis of the parallel evolution of seed shattering in weedy rice and …
Brachypodium As A Model For The Grasses: Today And The Future, Ana Lucia Caicedo, J. Brkljacic, E. Grotewold, R. Scholl, T. Mocker, D. F. Garvin, P. Vain, T. Brutnell, R. Sibout, M. Bevan, H. Budak, C. Gao, Y. Gu, S. P. Hazen, B. F. Holt Iii, S. -Y Hong, M. Jordan, A. J. Manzaneda, T. Michell-Olds, K. Mochida, L. A.J. Mur, C. -M. Park, J. Sedbrook, M. Watt, S. J. Zheng, J. P. Vogel
Brachypodium As A Model For The Grasses: Today And The Future, Ana Lucia Caicedo, J. Brkljacic, E. Grotewold, R. Scholl, T. Mocker, D. F. Garvin, P. Vain, T. Brutnell, R. Sibout, M. Bevan, H. Budak, C. Gao, Y. Gu, S. P. Hazen, B. F. Holt Iii, S. -Y Hong, M. Jordan, A. J. Manzaneda, T. Michell-Olds, K. Mochida, L. A.J. Mur, C. -M. Park, J. Sedbrook, M. Watt, S. J. Zheng, J. P. Vogel
Ana Lucia Caicedo
No abstract provided.
Molecular Evolution Of The Rice Blast Resistance Gene Pi-Ta In Invasive Weedy Rice In The Usa, Ana Lucia Caicedo, S. Lee, Y. Jia, D. R. Gealy, K. M. Olsen
Molecular Evolution Of The Rice Blast Resistance Gene Pi-Ta In Invasive Weedy Rice In The Usa, Ana Lucia Caicedo, S. Lee, Y. Jia, D. R. Gealy, K. M. Olsen
Ana Lucia Caicedo
The Pi-ta gene in rice has been effectively used to control rice blast disease caused by Magnaporthe oryzae worldwide. Despite a number of studies that reported the Pi-ta gene in domesticated rice and wild species, little is known about how the Pi-ta gene has evolved in US weedy rice, a major weed of rice. To investigate the genome organization of the Pi-ta gene in weedy rice and its relationship to gene flow between cultivated and weedy rice in the US, we analyzed nucleotide sequence variation at the Pi-ta gene and its surrounding 2 Mb region in 156 weedy, domesticated and …
Timing Is Everything: Early Degradation Of Abscission Layer Is Associated With Increased Seed Shattering In U.S. Weedy Rice, Ana Lucia Caicedo, C. S. Thurber, P. K. Helper
Timing Is Everything: Early Degradation Of Abscission Layer Is Associated With Increased Seed Shattering In U.S. Weedy Rice, Ana Lucia Caicedo, C. S. Thurber, P. K. Helper
Ana Lucia Caicedo
Background Seed shattering, or shedding, is an important fitness trait for wild and weedy grasses. U.S. weedy rice (Oryza sativa) is a highly shattering weed, thought to have evolved from non-shattering cultivated ancestors. All U.S. weedy rice individuals examined to date contain a mutation in the sh4 locus associated with loss of shattering during rice domestication. Weedy individuals also share the shattering trait with wild rice, but not the ancestral shattering mutation at sh4; thus, how weedy rice reacquired the shattering phenotype is unknown. To establish the morphological basis of the parallel evolution of seed shattering in weedy rice and …
Molecular Evolution Of The Rice Blast Resistance Gene Pi-Ta In Invasive Weedy Rice In The Usa, Ana Caicedo, S. Lee, Y. Jia, D. Gealy, K. Olsen
Molecular Evolution Of The Rice Blast Resistance Gene Pi-Ta In Invasive Weedy Rice In The Usa, Ana Caicedo, S. Lee, Y. Jia, D. Gealy, K. Olsen
Biology Department Faculty Publication Series
The Pi-ta gene in rice has been effectively used to control rice blast disease caused by Magnaporthe oryzae worldwide. Despite a number of studies that reported the Pi-ta gene in domesticated rice and wild species, little is known about how the Pi-ta gene has evolved in US weedy rice, a major weed of rice. To investigate the genome organization of the Pi-ta gene in weedy rice and its relationship to gene flow between cultivated and weedy rice in the US, we analyzed nucleotide sequence variation at the Pi-ta gene and its surrounding 2 Mb region in 156 weedy, domesticated and …
Genomic Patterns Of Nucleotide Diversity In Divergent Populations Of U.S. Weedy Rice, Michael Reagon, Carrie Thurber, Brian Gross, Kenneth Olsen, Yulin Jia, Ana Caicedo
Genomic Patterns Of Nucleotide Diversity In Divergent Populations Of U.S. Weedy Rice, Michael Reagon, Carrie Thurber, Brian Gross, Kenneth Olsen, Yulin Jia, Ana Caicedo
Biology Department Faculty Publication Series
Background: Weedy rice (red rice), a conspecific weed of cultivated rice (Oryza sativa L.), is a significant problem throughout the world and an emerging threat in regions where it was previously absent. Despite belonging to the same species complex as domesticated rice and its wild relatives, the evolutionary origins of weedy rice remain unclear. We use genome-wide patterns of single nucleotide polymorphism (SNP) variation in a broad geographic sample of weedy, domesticated, and wild Oryza samples to infer the origin and demographic processes influencing U.S. weedy rice evolution. Results: We find greater population structure than has been previously reported for …
Genomic Patterns Of Nucleotide Diversity In Divergent Populations Of U.S. Weedy Rice, Michael Reagon, Carrie S. Thurber, Brian L. Gross, Kenneth M. Olsen, Yulin Jia, Ana Lucia Caicedo
Genomic Patterns Of Nucleotide Diversity In Divergent Populations Of U.S. Weedy Rice, Michael Reagon, Carrie S. Thurber, Brian L. Gross, Kenneth M. Olsen, Yulin Jia, Ana Lucia Caicedo
Ana Lucia Caicedo
Background: Weedy rice (red rice), a conspecific weed of cultivated rice (Oryza sativa L.), is a significant problem throughout the world and an emerging threat in regions where it was previously absent. Despite belonging to the same species complex as domesticated rice and its wild relatives, the evolutionary origins of weedy rice remain unclear. We use genome-wide patterns of single nucleotide polymorphism (SNP) variation in a broad geographic sample of weedy, domesticated, and wild Oryza samples to infer the origin and demographic processes influencing U.S. weedy rice evolution. Results: We find greater population structure than has been previously reported for …
Exploiting Position Effects And The Gypsy Retrovirus Insulator To Engineer Precisely Expressed Transgenes, Michele Markstein, Chrysoula Pitsouli, Christians Villalta, Susan E. Celniker, Norbert Perrimon
Exploiting Position Effects And The Gypsy Retrovirus Insulator To Engineer Precisely Expressed Transgenes, Michele Markstein, Chrysoula Pitsouli, Christians Villalta, Susan E. Celniker, Norbert Perrimon
Michele Markstein
A major obstacle to creating precisely expressed transgenes lies in the epigenetic effects of the host chromatin that surrounds them. Here we present a strategy to overcome this problem, employing a Gal4-inducible luciferase assay to systematically quantify position effects of host chromatin and the ability of insulators to counteract these effects at phiC31 integration loci randomly distributed throughout the Drosophila genome. We identify loci that can be exploited to deliver precise doses of transgene expression to specific tissues. Moreover, we uncover a previously unrecognized property of the gypsy retrovirus insulator to boost gene expression to levels severalfold greater than at …