Developmental Biology Commons^™

Larval Anatomy Of Monotypic Painted Ant Nest Frogs Lithodytes Lineatus Reveals Putative Homoplasies With The Leptodactylus Pentadactylus Group (Anura: Leptodactylidae), Filipe A.C Do Nascimento, Rafael O. De Sá, Paulo C. De A. Garcia

Biology Faculty Publications

The morphological diversity of anuran larvae made them an important source of information for evolutionary and systematic studies. For the monotypic frog genus Lithodytes, which has an interesting taxonomic history, including its past synonymizing with Adenomera and its placement as a subgenus of Leptodactylus, the information provided from its larvae can help to understand its systematics interrelationships and also provide insights about its evolutionary trajectories. Herein, we provide a detailed description of the larval morphology of Lithodytes lineatus, including novel data of internal morphology (buccopharyngeal cavity and skeleton), and discuss some morphological and evolutionary aspects in relation …

Patterns Of Morphological Plasticity In Metriaclima Zebra And Danio Rerio Suggest Differently Canalized Phenotypes Due To Form-Function Relationships, Dylan Jockel

Masters Theses

In order to ascertain the degree of compatibility in developmental restructuring and behavioral plasticity between two fish species frequently made subject of laboratory research (Metriaclima zebra & Danio rerio), alternative trophic niche exposure experiments utilizing novel three-prong feeding treatments were conducted to obtain morphometric data, which demonstrated both species do bear some degree of plasticity. The results are somewhat complicated by differences in locality of detectable restructuring, which may be due to disparity in the form-function relationship for each species’ lineage. Each is notable in the manner of respective species’ jaw protrusion, as it is driven by anterior …

Phylogenomics And Geometric Morphometrics Define Species Flocks Of Snowtrout (Teleostei: Schizothorax) In The Central Himalayas, Binod Regmi

Graduate Theses and Dissertations

Schizothorax (Snowtrout) is a genus of medium-sized minnows (Cypriniformes) inhabiting glacier-fed streams, rivers, and lakes in the Himalayas. There are more than 30 species of Schizothorax across the region. The speciation and diversity of the Snowtrout in the vast hinterlands of the Himalayan Region has not been fully explored. Three species in Lake Rara, Western Nepal are considered a species flock, comprising endemic ecotypes that are morphologically differentiated and reproductively isolated.

My dissertation research examined the diversity of Schizothorax in the Central Himalayan region and evolutionary relationships among species distributed in the Tibet, Central and Southeast Asia. Chapter I describes …

Do Enallagma Exsulans From Streams And Lakes Show Patterns Of Divergence?, Savannah Rae Graham

Graduate Theses and Dissertations

Divergent selection across heterogenous environments could lead to adaptive divergence in populations resulting in potential local adaption. These populations have phenotypic differences that are fitness related and make native individuals more fit than non-native individuals. My research focuses on a species of damselfly, Enallagma exsulans, to explore local adaptation and morphological differences as a result of divergent selection or plasticity. My first study explored potential local adaptation of wild caught stream and lake E. exsulans using a reciprocal transplant design, a classic approach for this objective. The stream and lake sites chosen were on a small spatial scale allowing for …

The Effects Of Temperature On Maternal Investment Of Ovary Tissue In The Fish Species Danio Rerio, Noah R. Dillon, Alyce Demarais

Summer Research

The impact of climate change is predicted to increase water temperatures by 2 °C on average globally within the next century. As ectotherms, fish metabolism is directly connected to ambient water temperature. With a shift in metabolism due to temperature change, physiological processes like maternal investment may be affected. This study is an ecological and developmental investigation of the impacts of shifting environmental temperature conditions on the reproductive traits of the fish species Danio rerio. This study investigated Danio rerio under different temperatures to measure maternal investment. Female Danio rerio were exposed to temperature treatments 20-30 °C. Ovary tissue …

Butyl Benzyl Phthalate (Bbp) Induces Caudal Defects During Embryonic Development, Nicole M. Roy, Ewelina Zambrzycka, Jenna Santangelo

Biology Faculty Publications

Butyl benzyl phthalate (BBP) is commonly added during the manufacturing of plastics to increase flexibility and elasticity. However, BBP leaches off of plastic and environment presence has been detected in soil, groundwater and sediment potentially effecting organisms in the environment. Given the widespread uses of BBP in household, consumer goods and the presence of BBP in the environment, studies on developmental toxicity are needed. Here, we use a zebrafish model to investigate the early developmental toxicity of BBP. We treated gastrula staged embryos with increasing concentrations of BBP and noted concentration-dependent defects in caudal tail development, but the effect was …

An Investigation Of Juvenile Alewife (Alosa Pseudoharengus) Habitat Use And Growth Using Natural Markers, Gregory Norman Labonte Ms

All Student Scholarship

This research attempts to connect patterns in growth and migration of an anadromous species. The goal of this research was to understand habitat movements and growth of juvenile alewives (Alosa pseudoharengus) in the Penobscot Estuary and Bay through the use of otolith microchemistry, otolith growth increments, and a laboratory stable isotope turnover study. Understanding the connection between growth and movement of juvenile alewives may lead to more accurate and sophisticated conservation and restoration methods for anadromous species.

Unexpected Phylogenetic Positions Of The Genera Rupirana And Crossodactylodes Reveal Insights Into The Biogeography And Reproductive Evolution Of Leptodactylid Frogs, Antoine Foquet, Boris Leonardo Blotto, Maximiliano Manuel Maronna, Vanessa Kruth Verdade, Flora Acuña Juncá, Rafael O. De Sá, Miguel Trefaut Rodrigues

Biology Faculty Publications

Despite major progress in deciphering the amphibian tree of life by molecular phylogenetics, we identified two questions remaining to be answered regarding relationships within Hyloidea, the clade of South American origin that comprises most extant anuran diversity. A few genera like Rupirana and Crossodactylodes have enigmatic phylogenetic positions, and relationships among major lineages within some families like Leptodactylidae remain ambiguous. To resolve these specific questions we used two approaches (1) a complete matrix approach representing >6.6 kb, including most major Hyloidea lineages (61 terminals) combining different methods of phylogenetic reconstruction and measures of node support; and (2) a supermatrix approach …

Phenotypic Plasticity Of Rattlesnake Trophic Morphology, Matthew Thomas Smith

Graduate Theses and Dissertations

The trophic morphology of gape-limited predators constrains the shape and size of prey items they can ingest. Trophic morphology consists of any morphological feature that is involved in the handling and ingestion of food. Diet has a profound effect on the morphology of many gape-limited predators. Identifying how prey type and resource level affect the morphology of different populations is an essential step in understanding the mechanisms contributing to patterns of morphological diversity. Species interactions (Chapter 1) induce plasticity in morphology that can lead to increased fitness, morphological divergence, and eventually speciation.

In Chapter 2, a laboratory study tested the …

Interspecies Comparison Of Αii-Spectrin Abundance Between Chinook Salmon And Steelhead, Brielle D. Kemis, Ann L. Miracle, Katie A. Wagner, Christa M. Woodley

STAR Program Research Presentations

Salmonids, such as Chinook salmon (Oncorhynchus tshawytscha) and steelhead (O. mykiss), are a staple economic, recreational, tribal, and environmental resource, yet many populations are unsustainable. This study was part of a broad scale effort to monitor the impact of downstream migration obstacles on juvenile salmonid health and survival, which is an essential step towards increasing Smolt-to-Adult Return ratios (SARs). The objective of this study was to determine if juvenile Chinook salmon and steelhead exhibit differing quantities of alphaII-Spectrin Breakdown Products (SBDPs) over two consecutive spring migration periods, indicative of neurogenesis rate and/or biological response to head …

Morphological And Molecular Defects In Zebrafish Embryos (Danio Rerio) After Chromated Copper Arsenate Exposure, Kerry Jean Lee

Biological Sciences Theses & Dissertations

This investigation identified the morphological defects caused by different concentrations of chromium, copper, and arsenic (CCA) on the developing zebrafish embryo; in addition to defects caused by the three combined components (complete CCA). A change in the DNA of the developing embryos in response to the three components of CCA and complete CCA was also determined. Early Dania rerio embryos were cultured for five days with various concentrations of CCA components and complete CCA. Morphological defects were assessed with light microscopy and DNA fragmentation was determined by agarose gel electrophoresis. Embryonic exposure of D. rerio to sub lethal concentrations of …

The Tadpole Of Proceratophrys Avelinoi (Anura: Leptodactylidae), Rafael O. De Sá, José A. Langone

Biology Faculty Publications

The genus Proceratoprhys is poorly known. It consists of 14 currently recognized species (Frost, 2000) of medium-sized frogs distributed from northeastern Argentina and Paraguay to southeast Amazonia (Rondonia State), eastern and southern Brazil Proceratophrys avelinoi was described from Misiones, Argentina (Mercadal de Barrio and Barrio, 1993). The larval stage of this species is unknown. Herein, we describe the tadpole and the characteristics of the internal oral anatomy of P. avelinoi using scanning electron microscopy (SEM).

Factors Influencing Spatial Variability In Soil Nitrogen (N) Dynamics In N-Treated And Untreated Watersheds Of The Fernow Experimental Forest, West Virginia, Nikki Lenore Lyttle

Theses, Dissertations and Capstones

The central Appalachian region of the United States receives some of the highest inputs of nitrogen (N) due to acidic deposition in the nation. It is believed that these high could levels contribute to a decline in forest soils within the next 50 to 70 yrs. This study examines factors that influence spatial variability in N-treated and untreated watersheds of the Fernow Experimental Forest, Parsons, West Virginia. Within each of the two watersheds [WS4 untreated control, > 100 yr.; WS3 N-treated, acidified, clear cut, ~ 31 yr.], two 0.04 ha plots, one high N and one low N, were selected for …

Estructura Del Condrocráneo Y Esqueleto Visceral De Larvas De Pseudis Minuta (Anura, Pseudidae, E. O. Lavilla, Rafael O. De Sá

Biology Faculty Publications

The chondrocranium and visceral skeleton of Pseudis minuta tadpoles are described, based on a series of five larvae in stages 31 - 35 of Gosner (1960). Among their striking characters are the presence of peculiar articular surfaces between cornua trabeculae and suprarostral cartilage, the incomplete development of the orbital cartilage, the high fenestration of the floor of the cavum cranii, the fusion of posterior foramina, and the fusion of spicules 3 and 4 in the hyobranchial skeleton.

Development Of The Suprarostral Plate Of Pipoid Frogs, Rafael O. De Sá, Charles C. Swart

Biology Faculty Publications

The rostral region of nonpipoid tadpoles has two sets of cartilages, the cornua trabeculae and the suprarostral cartilages, whereas the rostral region in pipoid larvae is occupied by a single and continuous cartilage, the suprarostral plate. The homology of this region in pipoid and nonpipoids tadpoles has been controversial. We examined the early formation and development of the suprarostral plate using serially cross-sectioned specimens of Rhinophrynus, Xenopus, and Hymenochirus. We conclude that the cartilaginous structures present in the rostral area of pipoid and nonpipoid larvae are homologous. Furthermore, we found two different developmental patterns among pipoid larvae. The chondrocranium …

Características De La Osificación Craneal En Phyllomedusa Boliviana (Anura: Hylidae), Rafael O. De Sá, E. O. Lavilla

Biology Faculty Publications

RESUMEN. Dentro de la familia Hylidae disponemos de información sobre las secuencias de osificación craneal para menos del 4.0% de sus miembros. El presente trabajo describe la secuencia de osificación craneal de Phyllomedusa boliviana. El patrón general de osificación es similar a lo reportado para otras especies de la familia, pero existen algunas diferencias importantes tales como la osificación tardía de los próoticos y la osificación premetamórfica de neopalatino, vómer, cuadrado-yugal, pterigoides y columela. El lento desarrollo ulterior de la osificación resulta en juveniles con cráneos extensamente cartilaginosos.

ABSTRACT. Characteristics of the cranial ossification in Phyllomedusa …

Karyology And Morphometrics Of Three Species Of Akodon (Mammalia: Muridae) From Northwestern Argentina, Rubén M. Barquez, Daniel F. Williams, Michael A. Mares, Hugh H. Genoways

University of Nebraska State Museum: Mammalogy Papers

Chromosomal and morphometric studies were conducted on a sample from an assemblage of Akodon spp. occurring in various patterns of sympatry from the provinces of Catamarca, Jujuy, Salta, and Tucumán, Argentina. Results showed three distinct morphometric groups based upon size. Size also varied with age, but there were no significant differences in measurements of males and females. The three morphometric groups have distinct karyotypes. Akodon caenosus Thomas is the smallest of the three, and has a karyotype of 2n = 34, FN = 40. A. boliviensis tucumanensis J. A. Allen is intermediate in size and has 2n = 40, FN …