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Dosage compensation

2013

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Full-Text Articles in Molecular Biology

Investigation Of X Chromosome Recognition: The Role Of Small Rna In Drosophila Dosage Compensation, Debashish Unnikrishnan Menon Jan 2013

Investigation Of X Chromosome Recognition: The Role Of Small Rna In Drosophila Dosage Compensation, Debashish Unnikrishnan Menon

Wayne State University Dissertations

In humans and flies, females have two X chromosomes but males have one X chromosome and one Y chromosome. This leads to a fatal imbalance in X-linked gene expression in one sex. In mammals and in the fruit fly Drosophila, modulation of X chromosome expression is critical for survival. This process is termed dosage compensation. Flies increase expression from the male X chromosome two-fold. This is achieved by the Male Specific Lethal (MSL) complex, which consists of two large, non-coding RNA on the X transcripts (roX1 and roX2) and five proteins. The roX RNAs have a critical …

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Determination Of Dosage Compensation Of The Mammalian X Chromosome By Rna-Seq Is Dependent On Analytical Approach, Nathaniel K. Jue, Michael B. Murphy, Seth D. Kasowitz, Sohaib M. Qureshi, Craig J. Obergfell, Sahar Elsisi, Robert J. Foley, Rachel J. O’Neill, Michael J. O’Neill Dec 2012

Determination Of Dosage Compensation Of The Mammalian X Chromosome By Rna-Seq Is Dependent On Analytical Approach, Nathaniel K. Jue, Michael B. Murphy, Seth D. Kasowitz, Sohaib M. Qureshi, Craig J. Obergfell, Sahar Elsisi, Robert J. Foley, Rachel J. O’Neill, Michael J. O’Neill

Nathaniel Jue

Background: An enduring question surrounding sex chromosome evolution is whether effective hemizygosity in
the heterogametic sex leads inevitably to dosage compensation of sex-linked genes, and whether this compensation has been observed in a variety of organisms. Incongruence in the conclusions reached in some recent reports has been attributed to different high-throughput approaches to transcriptome analysis. However, recent reports each utilizing RNA-seq to gauge X-linked gene expression relative to autosomal gene expression also arrived at diametrically opposed conclusions regarding X chromosome dosage compensation in mammals.
Results: Here we analyze RNA-seq data from X-monosomic female human and mouse tissues, which are
uncomplicated …

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