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Molecular Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

2016

Ray Enke Ph.D.

Gene expression analysis protocol

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Full-Text Articles in Molecular Biology

Qpcr Analysis Of Differential Gene Expression (Wet Lab), Raymond A. Enke May 2016

Qpcr Analysis Of Differential Gene Expression (Wet Lab), Raymond A. Enke

Ray Enke Ph.D.

This class tested protocol will guide students through the steps for the following activities:
  • Analyze qPCR data to determine normalized gene expression for candidate genes identified from an RNA-Seq data set
  • Set up additional or repeat experimental qPCR reactions
  • Create a draft poster outlining the methods & data collected during the 6-week RNA-Seq analysis lab module


1st Strand Cdna Synthesis From Total Rna (Wet Lab), Raymond A. Enke May 2016

1st Strand Cdna Synthesis From Total Rna (Wet Lab), Raymond A. Enke

Ray Enke Ph.D.

This class tested protocol will guide students through the steps for the following activities:
Construct a cDNA library representative of total cellular messenger RNAs


Dissection & Rna Isolation From Embryonic Chicken Retina (Wet Lab), Raymond A. Enke May 2016

Dissection & Rna Isolation From Embryonic Chicken Retina (Wet Lab), Raymond A. Enke

Ray Enke Ph.D.

This class tested protocol will guide students through the steps for the following activities:
  • Dissect retina & cornea from developing chicken embryos
  • Extract total cellular RNA from tissues for downstream gene expression analysis