Rna Sequencing Analysis Of The Developing Chicken Retina, Christophe Langouet-Astrie*, Annamarie Meinsen*, Emily R. Grunwald*, Stephen Turner, Raymond A. Enke
Nov 2016
Rna Sequencing Analysis Of The Developing Chicken Retina, Christophe Langouet-Astrie*, Annamarie Meinsen*, Emily R. Grunwald*, Stephen Turner, Raymond A. Enke
Ray Enke Ph.D.
RNA sequencing transcriptome analysis using massively parallel next generation sequencing technology provides the capability to understand global changes in gene expression throughout a range of tissue samples. Development of the vertebrate retina requires complex temporal orchestration of transcriptional activation and repression. The chicken embryo (Gallus gallus) is a classic model system for studying developmental biology and retinogenesis. Existing retinal transcriptome projects have been critical to the vision research community for studying aspects of murine and human retinogenesis, however, there are currently no publicly available data sets describing the developing chicken retinal transcriptome. Here we used Illumina RNA sequencing …
Connecting Common Genetic Polymorphisms To Protein Function: A Modular Project Sequence For Lecture Or Lab, Christopher E. Berndsen, Byron H. Young, Quinlin Mccormick*, Raymond A. Enke
Oct 2016
Connecting Common Genetic Polymorphisms To Protein Function: A Modular Project Sequence For Lecture Or Lab, Christopher E. Berndsen, Byron H. Young, Quinlin Mccormick*, Raymond A. Enke
Ray Enke Ph.D.
Single nucleotide polymorphisms (SNPs) in DNA can result in phenotypes where the biochemical basis may not be clear due to the lack of protein structures. With the growing number of modeling and simulation software available on the internet, students can now participate in determining how small changes in genetic information impact cellular protein structure and function. We have developed a modular series of activities to engage lab or lecture students in examining the basis for common phenotypes. The activities range from basic phenotype test- ing/observation to DNA sequencing and simulation of protein structure and dynamics. We provide as an exam- …
Qpcr Analysis Of Differential Gene Expression (Wet Lab), Raymond A. Enke
May 2016
Qpcr Analysis Of Differential Gene Expression (Wet Lab), Raymond A. Enke
Ray Enke Ph.D.
This class tested protocol will guide students through the steps for the following activities:
- Analyze qPCR data to determine normalized gene expression for candidate genes identified from an RNA-Seq data set
- Set up additional or repeat experimental qPCR reactions
- Create a draft poster outlining the methods & data collected during the 6-week RNA-Seq analysis lab module
1st Strand Cdna Synthesis From Total Rna (Wet Lab), Raymond A. Enke
May 2016
1st Strand Cdna Synthesis From Total Rna (Wet Lab), Raymond A. Enke
Ray Enke Ph.D.
This class tested protocol will guide students through the steps for the following activities:
• Construct a cDNA library representative of total cellular messenger RNAs
Dissection & Rna Isolation From Embryonic Chicken Retina (Wet Lab), Raymond A. Enke
May 2016
Dissection & Rna Isolation From Embryonic Chicken Retina (Wet Lab), Raymond A. Enke
Ray Enke Ph.D.
This class tested protocol will guide students through the steps for the following activities:
- Dissect retina & cornea from developing chicken embryos
- Extract total cellular RNA from tissues for downstream gene expression analysis
Rna Gel & Spectrophotometer Analysis (Wet Lab), Raymond A. Enke
May 2016
Rna Gel & Spectrophotometer Analysis (Wet Lab), Raymond A. Enke
Ray Enke Ph.D.
This class tested protocol will guide students through the steps for the following activities:
- Determine quantity & purity of total RNA using a spectrophotometer
- Determine the quality of total RNA using native agarose gel electrophoresis (no formaldehyde required)
Bitter Taste Receptor Genotype/Phenotype Lab: A Modular Project Sequence For Lecture Or Lab, Raymond A. Enke
May 2016
Bitter Taste Receptor Genotype/Phenotype Lab: A Modular Project Sequence For Lecture Or Lab, Raymond A. Enke
Ray Enke Ph.D.
Single nucleotide polymorphisms (SNPs) in DNA can result in phenotypes where the biochemical basis may not be clear due to the lack of protein structures. With the growing number of modeling and simulation software available on the internet, students can now participate in determining how small changes in genetic information impact cellular protein structure and function. We have developed a modular series of activities to engage lab or lecture students in examining the basis for common phenotypes. The activities range from basic phenotype testing/observation to DNA sequencing and simulation of protein structure and dynamics. We provide as an example study …
