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Full-Text Articles in Molecular Biology
Rna Sequencing Analysis Of The Developing Chicken Retina, Christophe Langouet-Astrie*, Annamarie Meinsen*, Emily R. Grunwald*, Stephen Turner, Raymond A. Enke
Rna Sequencing Analysis Of The Developing Chicken Retina, Christophe Langouet-Astrie*, Annamarie Meinsen*, Emily R. Grunwald*, Stephen Turner, Raymond A. Enke
Ray Enke Ph.D.
RNA sequencing transcriptome analysis using massively parallel next generation sequencing technology provides the capability to understand global changes in gene expression throughout a range of tissue samples. Development of the vertebrate retina requires complex temporal orchestration of transcriptional activation and repression. The chicken embryo (Gallus gallus) is a classic model system for studying developmental biology and retinogenesis. Existing retinal transcriptome projects have been critical to the vision research community for studying aspects of murine and human retinogenesis, however, there are currently no publicly available data sets describing the developing chicken retinal transcriptome. Here we used Illumina RNA sequencing …
Dissection & Rna Isolation From Embryonic Chicken Retina (Wet Lab), Raymond A. Enke
Dissection & Rna Isolation From Embryonic Chicken Retina (Wet Lab), Raymond A. Enke
Ray Enke Ph.D.
This class tested protocol will guide students through the steps for the following activities:
- Dissect retina & cornea from developing chicken embryos
- Extract total cellular RNA from tissues for downstream gene expression analysis