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Full-Text Articles in Molecular Biology
In Vitro Selection Of Aptamers Against Avian Influenza Virus H5n1, Jingjing Zhao
In Vitro Selection Of Aptamers Against Avian Influenza Virus H5n1, Jingjing Zhao
Graduate Theses and Dissertations
Over $10 billion losses in the poultry industry were caused by avian influenza (AI) so far. Rapid and specific detection of avian influenza virus is urgently needed with the concerns over the outbreaks of highly pathogenic H5N1 influenza virus and cases of animal and human infection. Aptamers are oligonucleic acid or peptide molecules that bind a specific target molecule with good affinity. They show better thermal stability than antibodies. The goal of this research was to select DNA-aptamers as the specific recognition element of AI H5N1virus to be used in detection assays specific for field application. In this study, Systematic …
Design And Application Of Composite Rna Aptamers, Shengchun Wang
Design And Application Of Composite Rna Aptamers, Shengchun Wang
Legacy Theses & Dissertations (2009 - 2024)
aptamers are being developed as an essential tool in many fields of biological research. Their utility is not limited to being protein inhibitors; a lot of novel functions can be realized. However, in vivo application of RNA aptamers still faces many challenges. The aim of this dissertation is to design and apply composite aptamers in multiple expression and delivery systems to address some critical issues, such as correct folding, high level production, degradation by nucleases, excessive consumption of cellular resource and potential toxic effect.