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Full-Text Articles in Molecular Biology
Mouse Mast Cell Proteases: Induction, Molecular Cloning, And Characterization, Wei Chu
Mouse Mast Cell Proteases: Induction, Molecular Cloning, And Characterization, Wei Chu
Electronic Theses and Dissertations
Tryptase, a mast cell-specific serine protease with trypsin-like specificity, has been identified in a mouse mast cell line (ABFTL-6) based on it's enzymatic activity, inhibition properties, and cross-reactivity to a human mast cell tryptase antibody. The effects of fibroblast-conditioned medium and sodium butyrate on ABFTL-6 mast cell differentiation and tryptase expression have been examined. ABFTL-6 mouse mast cells undergo phenotypic changes upon culturing in media supplemented with fibroblast-conditioned media at 50% or 1 mM sodium butyrate. The induced cells increased in size, had larger and more metachromatic cytoplasmic granules, and increased their total cellular protein about four-fold. Tryptase activity increased …
The Role Of Queuine Deficient Trna In Translational Control, Kathryn Lenore Hart
The Role Of Queuine Deficient Trna In Translational Control, Kathryn Lenore Hart
Theses and Dissertations in Biomedical Sciences
The purpose of this research was to study the effect of the level of tRNA modification with respect to queuine on translational efficiency. Transfer RNA from neoplastic cells is generally deficient with regard to certain hypermodified nucleosides found in normal cells. The hypothesis that was examined is whether or not the altered efficiency of translation can be correlated to the presence of queuine hypomodified tRNA. The loss of queuine in the anticodon may change the interaction of the tRNA's anticodon with its codon. Therefore, the translational complex may stall looking for a rare tRNA, or the stability of the complex …