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Articles 1 - 13 of 13

Full-Text Articles in Molecular Biology

Characterization Of Two Temperature-Sensitive Mutants Of Escherichia Coli Exhibiting An Altered L22 Ribosomal Protein, Bonnie A. Burnette-Vick Aug 1991

Characterization Of Two Temperature-Sensitive Mutants Of Escherichia Coli Exhibiting An Altered L22 Ribosomal Protein, Bonnie A. Burnette-Vick

Electronic Theses and Dissertations

Analysis of E. coli strains SK1047 and SK1048 have shown them to be temperature-sensitive, protein-synthesis deficient. An alteration in ribosomal protein L22 was detected in both strains using two dimensional gel electrophoresis. Protein L22 was purified from both strains by reversed phase high performance liquid chromatography and from two dimensional electrophoretic gels. Purified ribosomal protein L22 was labeled by reductive methylation and used in 23S RNA binding assays with and without ribosomal protein L4. At the permissive temperature, protein L22 from SK1047 bound less efficiently than the control while protein L22 from SK1048 bound as efficiently as the control. At ...


Mouse Mast Cell Proteases: Induction, Molecular Cloning, And Characterization, Wei Chu May 1991

Mouse Mast Cell Proteases: Induction, Molecular Cloning, And Characterization, Wei Chu

Electronic Theses and Dissertations

Tryptase, a mast cell-specific serine protease with trypsin-like specificity, has been identified in a mouse mast cell line (ABFTL-6) based on it's enzymatic activity, inhibition properties, and cross-reactivity to a human mast cell tryptase antibody. The effects of fibroblast-conditioned medium and sodium butyrate on ABFTL-6 mast cell differentiation and tryptase expression have been examined. ABFTL-6 mouse mast cells undergo phenotypic changes upon culturing in media supplemented with fibroblast-conditioned media at 50% or 1 mM sodium butyrate. The induced cells increased in size, had larger and more metachromatic cytoplasmic granules, and increased their total cellular protein about four-fold. Tryptase activity ...


Identification Of Laminin Domains That Promote Tumor Cell Adhesion, Corby Freitag Apr 1991

Identification Of Laminin Domains That Promote Tumor Cell Adhesion, Corby Freitag

Life and Environmental Sciences Undergraduate Theses

The process of metastasis requires tumor cells to migrate through the basement membrane which consists of laminin, a glycoprotein which promotes the adhesion of HT-1080 fibrosarcoma cells. The present study was designed to test four peptides, corresponding to short sequences of the intact laminin molecule, for cell adhesion-promoting activity. By using three different cell migration assays, the four peptides were monitored for their cell adhesive-promoting activity. Results were obtained showing some cell adhesive-promoting activity from all four peptides.


Analysis Of R68.45 And R68.45-Met R-Primes By Physical And Genetic Criteria, Patricia Ann Mcqueney Jan 1991

Analysis Of R68.45 And R68.45-Met R-Primes By Physical And Genetic Criteria, Patricia Ann Mcqueney

Theses and Dissertations

No abstract provided.


Analysis Of Three Plasmids From Filamentous, Nitrogen-Fixing Cyanobacteria , Douglas Kevin Walton Jan 1991

Analysis Of Three Plasmids From Filamentous, Nitrogen-Fixing Cyanobacteria , Douglas Kevin Walton

Retrospective Theses and Dissertations

The cyanobacteria are an ancient and diverse group of prokaryotes capable of oxygenic photosynthesis. They display both unicellular and filamentous cell types and some have the ability to fix atmospheric dinitrogen, and the ability to differentiate specialized cells. In addition, many of the cyanobacteria possess plasmids. To date, only one plasmid, from a unicellular non-nitrogen-fixer, has been characterized in detail. The current lack of information concerning cyanobacterial plasmids, and their subsequent use as vectors, has hindered genetic analysis, particularly in the filamentous species;In this study, the properties of two plasmids and one plasmid origin of replication from filamentous, nitrogen-fixing ...


Changes In Gene Expression During Induction Of The Carbon Dioxide Concentrating Mechanism In Chlamydomonas Reinhardtii , Thomas Leslie Winder Jan 1991

Changes In Gene Expression During Induction Of The Carbon Dioxide Concentrating Mechanism In Chlamydomonas Reinhardtii , Thomas Leslie Winder

Retrospective Theses and Dissertations

The regulation of genes by CO[subscript]2 concentration in wild type and mutants of the unicellular green alga Chlamydomonas reinhardtii was studied. This and other algal species can photosynthesize efficiently under limiting concentrations of CO[subscript]2 due to the activities of a CO[subscript]2 concentrating mechanism and carbonic anhydrase. A cDNA copy of the gene that codes for a periplasmic carbonic anhydrase (perCA) was isolated and used to study regulation of perCA synthesis in a cell wall-deficient mutant (CW-15). Over-expression of perCA in CW-15, relative to wild type, was found at the levels of enzyme activity, protein ...


Molecular Mechanisms Controlling Bovine Lymphocyte Homing , Brad Thomas Bosworth Jan 1991

Molecular Mechanisms Controlling Bovine Lymphocyte Homing , Brad Thomas Bosworth

Retrospective Theses and Dissertations

The cDNA clones encoding for the bovine glycoproteins CD44 and LECAM-1 (lectin cell adhesion molecule-1) were isolated and sequenced. The predicted amino acid sequences of bovine CD44 and LECAM-1 had high identities with those of human and murine CD44 and LECAM-1. The high identities of the predicted amino acid sequences of these glycoproteins in the bovine, human and mouse suggest they may have similar functions in all three species. While the overall amino acid identities were high, bovine CD44 had an area of low identity with human and murine CD44 in the extracellular domain and the cytoplasmic tail of bovine ...


Study Of Secondary Structure Of Protein Sequences By Linear Algebra , Wei-Hua Hsieh Jan 1991

Study Of Secondary Structure Of Protein Sequences By Linear Algebra , Wei-Hua Hsieh

Retrospective Theses and Dissertations

Using encoding schemes, we study the relation between amino acids and protein structure in linear spaces;Local protein-structure prediction schemes use the amino acid sequences in a short subchain of a protein to predict the protein secondary structure of the middle residue of the chain;Two relatively reliable, objective and quantitative local prediction schemes are Robson et al.'s information theory method and and Qian & Sejnowski's neural network models. The latter achieved 64% accuracy for three-state predictions in 1989, the best prediction performance yet recorded;We assign to the 20 amino acids, through a one-to-one correspondence, the 20 columns (unit vectors) of the 20 x 20 identity matrix. Then any chain of k amino acids may be represented as a ...


Characterization Of A Potato Proteinase Inhibitor Ii Gene Whose Expression In Not Wound-Responsive , Sanggyu Park Jan 1991

Characterization Of A Potato Proteinase Inhibitor Ii Gene Whose Expression In Not Wound-Responsive , Sanggyu Park

Retrospective Theses and Dissertations

A potato Proteinase Inhibitor II gene (pin2T) was isolated and characterized both at the molecular level and the functional level. The open reading frame as well as the 5[superscript]' and 3[superscript]' flanking regions were sequenced. The coding region and 3[superscript]' flanking region showed 86% and 87% identity with those of the previously isolated wound-inducible potato Proteinase Inhibitor IIK gene (pin2K) sequence, respectively. However, the 5[superscript]' flanking region of the pin2T is highly homologous (91% identity) with that of pin2K from -767 to +29, relative to the transcription start site of the wound-inducible pin2K. The 5[superscript ...


Physical Mapping Of Rhizobium Meliloti And Glycine Max L. (Merr.) Using Pulsed-Field Gel Electrophoresis , Rhonda Jean Honeycutt Jan 1991

Physical Mapping Of Rhizobium Meliloti And Glycine Max L. (Merr.) Using Pulsed-Field Gel Electrophoresis , Rhonda Jean Honeycutt

Retrospective Theses and Dissertations

Partial physical maps of each of the three replicons of Rhizobium meliloti were constructed using restriction enzymes PacI, SpeI, and AseI. Using transverse alternating field gel electrophoresis (TAFE) in combination with very long pulse times, low agarose concentrations, and low field gradients, or in combination with PacI restriction enzyme digestions, the three replicons of R. meliloti 1021 were separated. Three bands were separated from undigested genomic DNA samples using TAFE; Southern hybridizations with representative cloned genes from each replicon revealed that each band corresponded to one of the replicons. The three bands were excised, digested with SpeI, and separated using ...


Effect Of 1,10-Phenanthroline On Dna Binding Of 1,25-Dihydroxyvitamin D Receptors And Characterization And Regulation Of Retinoic Acid Receptors In Rat Osteosarcoma Cells , Kevin Brady Atkins Jan 1991

Effect Of 1,10-Phenanthroline On Dna Binding Of 1,25-Dihydroxyvitamin D Receptors And Characterization And Regulation Of Retinoic Acid Receptors In Rat Osteosarcoma Cells , Kevin Brady Atkins

Retrospective Theses and Dissertations

The effect of the chelator 1,10-phenanthroline on the DNA-binding of 1,25-dihydroxyvitamin D receptor was demonstrated. Increasing concentrations of the chelator, up to 3 mM, decreased receptor binding to DNA-cellulose. This effect was specific for the chelating function because the nonchelating analog, 1,7-phenanthroline, did not inhibit DNA-cellulose binding of the receptor. The cations, Cd[superscript]2+ and Zn[superscript]2+, were able to block chelator-mediated inhibition. These cations were unable to reverse the inhibition unless 1,10-phenanthroline was removed by dialysis;The rat osteosarcoma 17/2.8 cells were shown to express both the cellular retinoic acid binding ...


Study Of En At The Wx-844 Allele: Modifier Of En Excision, Weak En, And Transposition Of En , Sudhansu Dash Jan 1991

Study Of En At The Wx-844 Allele: Modifier Of En Excision, Weak En, And Transposition Of En , Sudhansu Dash

Retrospective Theses and Dissertations

The wx-844 allele contains a fully active and complete En transposable element inserted within the Wx gene. The mutability of this allele is very high and the somatic mutability is seen in the endosperm as dark staining Wx sectors in a background of non-staining wx regions;The very high coarse pattern of waxy mutability is modified into a reduced fine pattern in the presence of the I-102 element located within the A gene in the non-autonomously mutable a-m(r) 102 allele. The modifying effect of the I-102 element on En induced excision has been found to be a general phenomenon ...


Avena Phytochrome Mrna: In Vivo Localization, Degradation Products, And Half-Life , Kevin Andrew Seeley Jan 1991

Avena Phytochrome Mrna: In Vivo Localization, Degradation Products, And Half-Life , Kevin Andrew Seeley

Retrospective Theses and Dissertations

The purpose of this research is to determine the role that post-transcriptional processes play in the regulation of phytochrome (phy) steady-state messenger RNA (mRNA) levels. The control of etiolated phytochrome (phyA) and chlorophyll a/b binding protein (cab) gene transcription and steady-state mRNA levels are known to be regulated by the phyA signal transduction pathway. I performed a number of control experiments that indicate the degradation products detected in northern analysis of phyA mRNA were produced in vivo and were not an artifact of RNA isolation techniques. I have also shown that infrared (IR) irradiation has no appreciable effect on ...