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Articles 1 - 7 of 7
Full-Text Articles in Molecular Biology
Plant Rac-Like Gtpases Are Activated By Auxin And Mediate Auxin-Responsive Gene Expression, L. Z. Tao, Alice Cheung, H. M. Wu
Plant Rac-Like Gtpases Are Activated By Auxin And Mediate Auxin-Responsive Gene Expression, L. Z. Tao, Alice Cheung, H. M. Wu
Alice Cheung
The auxin indole-3-acetic acid is a key plant hormone essential for a broad range of growth and developmental processes. Here, we show that auxin activates Rac-like GTPases (referred to as Rac/Rop GTPases), and they in turn stimulate auxin-responsive gene expression. In particular, we show that overexpressing a wild-type tobacco Rac/Rop GTPase, NtRac1, and its constitutively active mutant form activates auxin-responsive gene expression. On the other hand, overexpressing dominant-negative NtRac1 and Rac-negative regulators, or reducing the endogenous NtRac1 level, suppresses auxin-induced gene expression. Furthermore, overexpression of NtRac1 activity or suppression of its expression in transgenic seedlings induces phenotypes that are similar …
Electrically Mediated Delivery Of Vector Plasmid Dna Elicits An Antitumor Effect, L. Heller, D. Coppola
Electrically Mediated Delivery Of Vector Plasmid Dna Elicits An Antitumor Effect, L. Heller, D. Coppola
Bioelectrics Publications
In vivo electroporation is an efficient means of increasing plasmid DNA delivery to normal tissues, such as skin and muscle, as well as directly to tumors. In the experiments described here, plasmid DNA was delivered by in vivo electroporation to B16 mouse melanomas using two very different pulsing protocols. Reporter expression increased 21- or 42-fold, respectively with electroporation over injection alone. The growth of experimental melanomas with an approximate diameter of 4 mm on the day of treatment was monitored after electroporation delivery of reporter plasmid DNA. Remarkably, short-term complete regressions using one of these pulsing protocols occurred in up …
The Regulation Of Actin Organization By Actin-Depolymerizing Factor In Elongating Pollen Tubes, C. Y. Chen, E. I. Wong, L. Vidali, A. Estavillo, P. K. Hepler, H. M. Wu, Alice Cheung
The Regulation Of Actin Organization By Actin-Depolymerizing Factor In Elongating Pollen Tubes, C. Y. Chen, E. I. Wong, L. Vidali, A. Estavillo, P. K. Hepler, H. M. Wu, Alice Cheung
Alice Cheung
Pollen tube elongation is a polarized cell growth process that transports the male gametes from the stigma to the ovary for fertilization inside the ovules. Actomyosin-driven intracellular trafficking and active actin remodeling in the apical and subapical regions of pollen tubes are both important aspects of this rapid tip growth process. Actin-depolymerizing factor (ADF) and cofilin are actin binding proteins that enhance the depolymerization of microfilaments at their minus, or slow-growing, ends. A pollen-specific ADF from tobacco, NtADF1, was used to dissect the role of ADF in pollen tube growth. Overexpression of NtADF1 resulted in the reduction of fine, axially …
Flexibility Of Biv Tar-Tat: Models Of Peptide Binding, M Hsieh, E D. Collins, T Blomquist, Brooke Lustig
Flexibility Of Biv Tar-Tat: Models Of Peptide Binding, M Hsieh, E D. Collins, T Blomquist, Brooke Lustig
Faculty Publications, Chemistry
No abstract provided.
Trp-Dependent Auxin Biosynthesis In Arabidopsis: Involvement Of Cytochrome P450s Cyp79b2 And Cyp79b3, Yunde Zhao, Anna K. Hull, Neeru R. Gupta, Kendrick A. Goss, José Alonso, Joseph R. Ecker, Jennifer Normanly, Joanne Chory, John L. Celenza
Trp-Dependent Auxin Biosynthesis In Arabidopsis: Involvement Of Cytochrome P450s Cyp79b2 And Cyp79b3, Yunde Zhao, Anna K. Hull, Neeru R. Gupta, Kendrick A. Goss, José Alonso, Joseph R. Ecker, Jennifer Normanly, Joanne Chory, John L. Celenza
Jennifer Normanly
The plant hormone auxin regulates many aspects of plant growth and development. Although several auxin biosynthetic pathways have been proposed, none of these pathways has been precisely defined at the molecular level. Here we provide in planta evidence that the two Arabidopsis cytochrome P450s, CYP79B2 and CYP79B3, which convert tryptophan (Trp) to indole-3-acetaldoxime (IAOx) in vitro, are critical enzymes in auxin biosynthesis in vivo. IAOx is thus implicated as an important intermediate in auxin biosynthesis. Plants overexpressing CYP79B2 contain elevated levels of free auxin and display auxin overproduction phenotypes. Conversely, cyp79B2 cyp79B3 double mutants have reduced levels of IAA and …
Flexibility Of Biv Tar-Tat: Models Of Peptide Binding, M Hsieh, E D. Collins, T Blomquist, Brooke Lustig
Flexibility Of Biv Tar-Tat: Models Of Peptide Binding, M Hsieh, E D. Collins, T Blomquist, Brooke Lustig
Brooke S. Lustig
No abstract provided.
Fluorescence In Situ Hybridization On Sperm Using Alkaline Denaturation, L. J. Aridgides, M. Stacey, L. Brihn, D. Scott, C. Osgood
Fluorescence In Situ Hybridization On Sperm Using Alkaline Denaturation, L. J. Aridgides, M. Stacey, L. Brihn, D. Scott, C. Osgood
Bioelectrics Publications
Fluorescence in situ hybridization (FISH) permits the detection of unique DNA sequences of target DNA within single cells. FISH allows the identification of DNA sequences within individual cells and readily identifies genetic changes within a mixed population of cells. However, problems arise in maintaining nuclear morphology, particularly following harsh denaturation procedures where cells are heated to 80°C, causing the nuclear boundary to become blurred or the cell to completely burst. In an attempt to preserve cellular morphology and high hybridization efficiency, particularly in fresh tissue samples, we evaluated a FISH protocol using alkaline denaturation in place of heat.