Life Sciences Commons^™

Investigation Of A Locus On Chromosome 9 For Contributions To Pulmonary Hypertension Syndrome In Broilers, Sriram Krishnamoorthy

Graduate Theses and Dissertations

Although the ascites syndrome in chickens has been investigated for years, it continues to inflict financial losses to the world poultry industry. It is estimated that 8% of the 361 million broiler deaths are due to ascites leading to losses of millions of dollars annually. Efforts to curb the incidence of ascites are typically designed to slow early growth. This limits the birds' ability to show its true genetic potential and impacts later yields. In 1994 lines divergent for susceptibility to ascites were established from a commercial sire line through sibling selection of birds reared at local altitude after testing …

Feed Supplementation With Natural Extracts Of Cranberry And Its Efficacy On Campylobacter Colonization In Poultry, Ann Woo-Ming

Graduate Theses and Dissertations

Campylobacter spp. has been identified as one of the leading causative agents of food borne diarrheal illness. Epidemiological evidence has shown that poultry is the main source for human infection. Poultry are asymptomatic carriers of Campylobacter within their gastrointestinal tract, with colonization levels reaching 106-108 cfu/g cecal content. Surveys of domestic poultry flocks have estimated that approximately 90% of flocks are positive for Campylobacter colonization. Risk assessment studies have determined that by reducing levels of Campylobacter colonization during rearing, incidences of human infection will be significantly reduced. Currently there are no consistently effective treatments to eliminate Campylobacter from poultry flocks. …

Cotton Response To High Temperature Stress During Reproductive Development, Justin B. Phillips

Graduate Theses and Dissertations

Temperature is a primary controller of the rate of plant growth, developmental events, and fruit maturation. Increased temperatures from global climate change are projected to cause substantial losses in crop productivity by the end of the twenty-first century. Elevated temperatures affect all stages of cotton development, but the crop seems to be particularly sensitive to adverse temperatures during reproductive development. In Arkansas, temperature stress is considered to be one of the main factors affecting cotton yield. Environmental stress during floral development is a major reason for the disparity between actual and potential yields. Field and growth chamber studies were conducted …

Effects Of Management Strategies And Molecular Breeding Values On Cattle Performance And Carcass Traits, Benjamin Collins Williamson

Graduate Theses and Dissertations

Forty-eight Gelbvieh x Angus steers (265 ¡À 40 kg) were utilized to determine the relationships among molecular breeding values (MBV), steer performance, and carcass traits. Body weight (BW), hip height (HH), hip width (HW), exit velocity (EV; rate at which steers exited the squeeze chute and traversed 1.8 m) and body ultrasound measurements of steers were recorded at d 0, 93 and 154 of grazing mixed stockpiled endophyte-infected and -free tall fescue. Tissue samples were collected for genomic profiling (Igenity, Merial Limited, Duluth, GA). Steers were transported to the Oklahoma State U fed for 159 d, harvested and carcass parameters …

Territories,Territoriality, And Conservation Of The Louisiana Waterthrush And Its Habitat, The Watershed Of The Upper Buffalo National River, Leesia Cheryl Marshall

Graduate Theses and Dissertations

The Louisiana Waterthrush, Parkesia motacilla, is a migratory wood-warbler and breeding season resident of the eastern United States. Males defend breeding territories that extend linearly along clear, fast-flowing, gravel-bottomed, forest streams. Defense includes two song types, primary and extended song. As riverine specialists, birds rely upon aquatic invertebrates as prey and riparian habitat features for nesting. They use a unique foraging maneuver, leaf-pulling, that involves picking up or pulling a leaf from water, and turning it over to search for prey. Their relationship with riparian habitat introduces potential for Louisiana Waterthrushes to serve as indicators of stream health. The …

A Role Of Environmental Components In Autoimmune Vitiligo Of Smyth Line Chickens, Lei Dong

Graduate Theses and Dissertations

The Smyth line (SL) of chickens is a well-established animal model for the study of spontaneous autoimmune vitiligo, displaying all the characteristics of human vitiligo. In humans, phenolic derivative 4-tertiary butyl phenol (4-TBP) has been shown to trigger vitiligo in susceptible individuals. In vitiligo-prone SL-chickens, live turkey herpesvirus (HVT) infection at hatch was identified as the most effective trigger of SL-vitiligo expression. This dissertation examined the role of the environmental factors 4-TBP and HVT in the etiology of SL-vitiligo. Specifically, the generation of reactive-oxygen-species by melanocyte-containing feather tissue (MC-FT) and cultured melanocytes in response to 4-TBP exposure in vitro (Study …

Characterization Of The Vasotocin Receptor Subtype 4 (Vt4r) In The Brain And Pituitary Gland Of The Chicken, Gallus Gallus, Rajamani Selvam

Graduate Theses and Dissertations

The present study investigated the distribution of the vasotocin subtype four receptor (VT4R) in brain and pituitary gland of the chicken, Gallus gallus. The anterior pituitary cell types associated with the VT4R were also determined. Two polyclonal antibodies were raised in rabbit against a cocktail of peptides, 15 amino acids from the amino terminal region and 17 amino acids from the carboxy terminal region of VT4R receptor. The antibody was validated utilizing the Western blot and immunocytochemistry. A single band at 47KDa utilizing membrane protein extracts of chicken brain and pituitary tissues was shown for the VT4R antibody. A peptide …

Distribution And Control Of Herbicide-Resistant Italian Ryegrass (Lolium Perenne L. Ssp. Multiflorum Lam. Husnot) In Arkansas, James Walker Dickson

Graduate Theses and Dissertations

Italian ryegrass populations have evolved resistance to herbicides that producers rely on for weed control both in wheat and burn-down. The objectives of this research were to: test populations of Italian ryegrass from across Arkansas for resistance to glyphosate, diclofop, pinoxaden, and pyroxsulam; determine if there were any differences in control of 12 glyphosate-resistant populations in relation to glyphosate rate or application timing; determine the level of glyphosate resistance in one selected population versus a susceptible standard and a previously discovered glyphosate-resistant population; and determine the best options for controlling Italian ryegrass prior to planting crops. A total of 215 …

Interactions Of Nitric Oxide And Superoxide Pathways In Hyperglycemic Endothelial Cells, Steven Clay Rogers

Graduate Theses and Dissertations

Cardiovascular complications arising from diabetic hyperglycemia represents one of the leading causes of death and greatest public health challenges of modern societies. Despite state-of-the-art glucose control, diabetic patients remain at a markedly increased risk of cardiovascular disease. The loss of endothelial function (the development of diabetic endothelial dysfunction) has been implicated in the development of numerous diabetic cardiovascular diseases. The endothelial cell produces many vasoactive substances, hormones and cytoprotective biological factors. Endothelial cells are also involved in and affected by the initiation of inflammatory responses through the release and interaction of cytokines and other immune system molecules. Therefore, regulation of …

Internet Reviews: Livescience, John Creech

Library Scholarship

This column provides a review of LiveScience, a website created by TechMediaNetwork. The site is recommended for undergraduates seeking background information or professionals seeking quick facts.

The Effect Of Six Weeks Of Oral Echinacea Supplementation On Erythropoiesis, Nitric Oxide Production, And Exercise Performance, Tyler David Martin

Dissertations

The purpose of the present study was to investigate the effect of six weeks of oral Echinacea supplementation on serum erythropoietin (EPO) and erythropoietic status, plasma nitric oxide [NO], submaximal endurance exercise performance, and maximal oxygen uptake (V˙O_2max). Twenty-four males (mean ± SE): age = 25.2 ± 1.4 yr, height = 178.1 ± 1.4 cm, mass = 78.1 ± 1.6 kg, percent body fat = 12.7 ± 0.9 %, V˙O_max = 52.9 ± 0.9 mL·kg^-1·min^-1 were randomly grouped using a matched-pair, double-blind design and self-administered 8,000 mg·d^-1(5 × 400 mg × 4 …

Movement Ecology Of An Intercontinental Migratory Bird During Spring Stopover, Emily Beth Cohen

Dissertations

Movement ecology is a component of nearly all aspects of animal behavior and an animal’s decision to move is likely influenced by a complex combination of exogenous and endogenous factors. Therefore, an examination of the causes and consequences of organismal movement provides a conceptual framework for understanding complex behavioral strategies. My dissertation research is focused on the movement ecology of an intercontinental migratory songbird during spring migration. I adopted experimental approaches to study the factors influencing how a songbird migrant, red-eyed vireos (Vireo olivaceus), makes decisions in unfamiliar landscapes from the initiation of spring stopover.

I simulated the …

Foliar Symptoms Of Acute Ozone Injury And Exposure Response Characteristics Of Select Native Perennials, Susan Denise Frey

Graduate Theses and Dissertations

Twenty seven perennial species native to the Eastern Temperate Forests Level I Ecoregion were exposed to an acute ozone (O3) treatment consisting of a target peak O3 concentration of 2.0 ppm for 30 minutes in a closed chamber environment, during the summer of 2010. Plants were evaluated for visible foliar injury symptoms and symptoms were described and photographically documented. Ten of the 27 species developed visible foliar injury in which interspecific and intraspecific response to O3 was observed. A severity index was used to compare response to acute ozone exposure for the ten species displaying visible foliar injury. Species showing …

Rice (Oryza Sativa) Response To Low Glyphosate Rates As Influenced By Cultivar, Growth Stage, And Imazethapyr Applications, Jason R. Meier

Graduate Theses and Dissertations

Off-target movement of glyphosate onto rice is a perennial concern when rice is grown in close proximity to glyphosate-tolernat crops. If differential tolerance to sub-lethal rates of glyphosate exists among rice (Oryza sativa) cultivars, these cultivars could be utilized in breeding programs or glyphosate-drift sensitive areas. Field and greenhouse experiments were conducted in 2006 and 2007 to examine differences among rice cultivars in response to sub-lethal rates of glyphosate, and to examine imidazolinone-tolerant rice response to imazethapyr and sub-lethal rates of glyphosate applied sequentially to determine the potential for either herbicide to predispose rice to greater injury. In the field …

Effect Of Supplemental Trace Mineral Source (Organic Versus Inorganic) On Bull Semen Quality, Matt Rowe

Graduate Theses and Dissertations

Studies indicate that organic forms of trace minerals can improve cow reproductive performance, particularly during periods of stress. However, limited information is available on the effects of organic trace mineral supplementation on bull fertility. The objective of this study was to evaluate the effect of organic versus inorganic trace mineral supplementation on bull semen quality before and after freezing, as measured by computer-assisted sperm analysis (CASA). Angus and Balancer bulls were assigned to inorganic (n = 9) and organic (n = 10) trace mineral treatments, based on initial semen quality, breed, body weight, and age. The bulls were maintained in …

Earthworms Of Arkansas And Contributions Of Earthworm Evolutionary Origin And Ecological Group To Nitrogen Cycling In A Model Soil And Tall Fescue System, Peter John Tomlinson

Graduate Theses and Dissertations

Evolutionary origin and ecological niche may impact how earthworm species affect N cycling. Tall fescue (Lolium arundinaceum (Schreb) S. J. Darbysh) infection with the toxic endophyte Neotyphodium coenophialum Glenn, Bacon & Hanlin (E+) may influence earthworm communities in soil compared to non-toxic endophyte (NE+) infected tall fescue. Conversely, different earthworms may contribute different amounts of N to E+ vs. NE+ tall fescue. A field survey was conducted from January 2007 to January 2008 to determine the impact of E+ and NE+ tall fescue, planting date, and seasonal variables, e.g. soil temperature and moisture, on earthworm species, abundances, and population …

Determination Of Poultry Litter's Nitrogen-Fertilizer Value For Winter Wheat Production, Brett Lee Gordon

Graduate Theses and Dissertations

Nitrogen (N) availability in poultry litter (PL) applied in the fall as a nutrient source for winter wheat (Triticum aestivum L.) has not been adequately characterized. Our objective was to determine a potentially available N (PAN) coefficient based on the inorganic-N fertilizer equivalence (FEQ) of fall-applied PL to winter wheat via field and laboratory research. Wheat grown at six site-years received 0, 84 and 168 kg N ha-1 as PL applied preplant and/or at-planting. The FEQ of PL-N was based on growth and yield responses of wheat fertilized with 22 to 191 kg N ha-1 applied in late winter as …

Characterization And Molecular Analysis Of University Of Arkansas Peach, Prunus Persica (L.) Batsch, Flesh Types And Development Of A Post-Harvest Evaluation Protocol For Arkansas Peach And Nectarine Genotypes, Paul James Sandefur

Graduate Theses and Dissertations

This study determined the effect of pre-cold storage treatment on P. persica genotypes, developed a protocol for the evaluation of breeding selection and cultivar storage performance, and provided information on post-harvest performance of these individuals. Additionally, characterization of the peach and nectarine flesh types, validation of endopolygalacturonase (endoPG) DNA markers, and investigation of endoPG marker allele combinations associated with the slow-melting-flesh (SMF) trait were done.

Fruit from 30 individuals were harvested at minimum- and well-mature states. After conditioning for 24 h at 20 ◦C, all 2010 fruit were exposed to 2 min of 1 ◦C 100 ppm chlorinated hydro-cooling, a …

Effect Of Soyasaponin Fractions On Human Colon Cancer Cells, Joseph Tyler Salyer

Graduate Theses and Dissertations

Group B saponins, the predominant form of saponins in heat-treated soy products, have been shown to possess hypocholesterolemic, antimutagenic, and anticarcinogenic properties. Previous studies have evaluated crude mixtures of soyasaponins, but studies evaluating a single purified soyasaponin as an anticarcinogenic agent are limited. The goal of this study is to examine the effects of purified soyasaponins I and III as well as their aglycone form, soyasapogenol B, as anticarcinogenic agents on the human colon adenocarcinoma cell line Caco-2. Experiments were conducted to determine the effects of purified soyasaponins on cell proliferation, Protein Kinase C (PKC) activity, and cell morphology in …

In Vitro Selection Of Aptamers Against Avian Influenza Virus H5n1, Jingjing Zhao

Graduate Theses and Dissertations

Over $10 billion losses in the poultry industry were caused by avian influenza (AI) so far. Rapid and specific detection of avian influenza virus is urgently needed with the concerns over the outbreaks of highly pathogenic H5N1 influenza virus and cases of animal and human infection. Aptamers are oligonucleic acid or peptide molecules that bind a specific target molecule with good affinity. They show better thermal stability than antibodies. The goal of this research was to select DNA-aptamers as the specific recognition element of AI H5N1virus to be used in detection assays specific for field application. In this study, Systematic …

Proteome Based Development Of Novel Affinity Tail For Immobilized Metal Affinity Chromatography And Hydrophobic Interaction Chromatography, Neha Tiwari

Graduate Theses and Dissertations

At industrial scale, reducing the step in purification and recovery is desired; this not only decreases the cost but also increases the yield. Hydrophobic Interaction Chromatography (HIC) and Immobilized Metal Affinity Chromatography (IMAC) both are not harsh on biological structure or activity of proteins; also both the techniques are economical and therefore a suitable choice at industrial level. This dissertation comprises of three parts. Purpose for the Part I was to identify and characterize Escherichia coli proteins which display affinity towards both IMAC and Hydrophobic Interaction Chromatography (HIC). Co (II) IMAC was chosen as the primary capture step, followed by …

Host-Virus Interactions Of Infectious Laryngotracheitis Virus Infection In Cultured Cells, Jeong Yoon Lee

Graduate Theses and Dissertations

Infectious laryngotracheitis virus (ILTV; Gallid herpesvirus 1) causes upper respiratory diseases in mainly chickens and exhibits 90-100% of high morbidity and up to 70% of mortality, resulting in huge economic losses in the poultry industry worldwide.

To study host-ILTV interactions, the changes in genome-wide gene expressions in response to wild-type and vaccine ILTV infections in primary chicken embryo lung cells were investigated using microarray analysis. Results provide crucial insights into host cell pathogenic and immunogenic responses against wild-type and vaccine ILTV infections. Using microarray method and Ingenuity Pathway Analysis (IPA) bioinformatics tool, 273 and 306 differentially expressed genes were identified …

Can Antioxidant-Rich Berries Improve Risk Factors Associated With Cardiovascular Disease In Postmenopausal Smokers?, Jordan Alaine Teeple

Graduate Theses and Dissertations

The purpose of this study was to examine the effects of smoking on risk factors associated with cardiovascular disease (CVD) and determine the efficacy of antioxidant-rich berries in ameliorating these risk factors in postmenopausal smokers. Menopause and cigarette smoking have been identified as major risk factors for CVD due to a decrease in antioxidant protection, increase in inflammation and oxidative stress, and adverse changes in serum lipids. Antioxidants, specifically flavonoid compounds found in blackberries and blueberries, have been shown to prevent low-density lipoprotein cholesterol oxidation and thus prevent downstream inflammation and oxidative stress. Healthy, postmenopausal nonsmokers (n=14) and smokers (n=31) …

Improvements On Just-About-Right (Jar) Scales As Product Optimization Tools Using Kano Modeling Concepts, Bangde Li

Graduate Theses and Dissertations

Attribute Kano characteristics are useful in product design to prioritize development efforts. However, attribute Kano characteristics have not been discussed and applied to product optimization when using Just-About-Right (JAR) scales. Product optimizations without identifying attributes Kano characteristics can be misleading. The two objectives in this research were: 1. Determine attribute Kano categories using a modified classic Kano classification methodology. 2. Propose a method to measure attribute performance and identify attribute Kano characteristics to direct product optimization. Two methodologies of attribute Kano classification were investigated. In experiment one, a modified classic Kano methodology was employed to determine attribute Kano categories through …

Effect Of Nutrient Supplements On Cucumber Fermentation By Lactic Acid Bacteria, Shruti Tripuraneni

Graduate Theses and Dissertations

Lactic acid bacteria (LAB) are important industrial microorganisms involved in fermentation of food and beverage products. The strict fermentative growth of LAB has complex requirements of various nutrients including amino acids, vitamins and minerals. Information about the effect of these nutrients on the growth of LAB in cucumber fermentation is not readily available. It is evident, from previous research that certain nutrients like; leucine, isoleucine, tryptophan, valine, biotin, nicotinic acid, pantothenic acid, riboflavin, manganese and magnesium are beneficial for LAB growth, but are not provided in sufficient quantities by the cucumber in the brine. The objective of this study was …

The Incidence Of Plexiform Lesion Formation In Lines Divergently Selected For Ascites, James Mason

Graduate Theses and Dissertations

Complex Vascular Lesions, (CVL) are a common vascular change associated with idiopathicpulmonary arterial hypertension (IPAH). CVL have been characterized in studies using animal models involving, but not limited to, Sprague-Dawley rats and poultry (Abe et al., 2010; Wideman et al., 2011).IPAH has been documented in poultry although no connection has been made with CVLs. The current study characterizes CVL in the lungs from broilers derived from lines divergently selected for 15 generations for ascites susceptibility under conditions of simulated high altitude. The ascites RES and SUS lines were sampled for CVL incidence over time. Both lines were reared in a …

Protein Utilization And Requirements In Broiler Breeders, Ricardo David Ekmay

Graduate Theses and Dissertations

Advances in the genetics of poultry have created unintended consequences to the broiler breeder industry. Excessive weight has been shown to have many negative effects, including double hierarchies, multiple ovulations, and fatty liver. In an effort to curb excessive body weight gain, both the protein requirements and feeding strategies of broiler breeders are being revisited. A series of studies were conducted to determine the protein and amino acid requirements of broiler breeders during the production phase and to determine the interrelationship between protein turnover and egg formation. In the first trial, a 2x3x3 factorial study was conducted to determine the …

The Study Of The Parasitic Fauna In Sciaenops Ocellatus, Lower Laguna Madre, Texas, Veronica Abrigo

Theses and Dissertations - UTB/UTPA

Parasitological investigations provide information on ecosystem health because parasite prevalence and abundance are influenced by biotic and abiotic environmental changes. This survey classified the parasitic fauna of red drum, Sciaenops ocellatus, in the Lower Laguna Madre, TX. Fish (n = 239) were collected from four study sites (May 2005 - June 2006). Parasite identification consisted of digenean and monogenean trematodes, cestodes, larval nematodes, copepods, and protozoans. Parasite prevalence and host length was positively correlated from South Bay (r=0.473), Port Isabel (r=0.533), Arroyo(r=0.314), and Port Mansfield (r=0.630). Significant differences (p<0.001) between zone and parasite prevalence from Arroyo site indicated a higher prevalence of protozoa and monogeneans and reduced prevalence of digeneans. Statistical analyses indicated the greatest difference between Port Isabel and Arroyo sites ( divided by 2= 48.195; p<0.001; df= 14), and the least difference between South Bay and Arroyo sites ( divided by 2= 20.847; p= 1.06; df= 14).

Analyzing Spring Freeze Impacts On Deciduous Forest Productivity Using Modis Satellite Imagery, Karl Lintvedt

Graduate Theses and Dissertations

The impacts of an April 2007 spring freeze event on the productivity of deciduous broadleaf forest were analyzed using geographic information system (GIS) tools. Forest productivity was modeled using the Enhanced Vegetation Index (EVI), as recorded by the Moderate Resolution Imaging Spectroradiometer (MODIS) satellite sensor. Measures of spatial autocorrelation were used to quantify the degree of spatial congruence between a map depicting the severity of the freeze event, and maps modeling forest productivity throughout the year. The results show a geographic correlation between the unseasonably low minimum temperatures sustained during the freeze and the unusually low forest productivity that followed. …

Detection Of Two Pathogens Of High Importance To The National Poultry Improvement Plan: Salmonella Spp. And Mycoplasma Spp., Robin Levi Jarquin

Graduate Theses and Dissertations

In 1935, the National Poultry Improvement Plan (NPIP) was created to control Salmonella gallinarum and Salmonella pullorum . These two pathogens were devastating economically for poultry producers. Through cooperative efforts using vaccination and strict biosecurity, these two pathogens were eradicated from the United States. Currently, the NPIP program is targeting two other poultry pathogens, Salmonella enterica serovar Enteritidis and Mycoplasma . In the broiler industry it targets 2 specific Mycoplasma species (synoviae, gallisepticum). Vaccinations for these bacteria are available, but are not fully effective at controlling all strains and serovars. Thus, constant monitoring systems and strict biosecurity measures are necessary …