Life Sciences Commons^™

Relationship Among Genetic And Physiological Biomarkers, And Horse Characteristics, Casey Orr

Graduate Theses and Dissertations

The objective of this study was to evaluate relationships among genetic and physiological biomarkers and phenotypic characteristics in horses. Three breeds (Quarter Horse, Thoroughbreds, Miniatures), and five genes of interest (leptin, ghrelin, cytochrome P450, glucocorticoid receptor, and lactate dehydrogenase) were the basis of the investigation. Genomic DNA was extracted from buffy coat samples of Quarter Horses (n = 21), Thoroughbreds (n = 26), and Miniatures (n = 34). Data were analyzed using MIXED MODEL procedures of SAS, with the main effects of breed and SNP genotype. Horse was the experimental unit, and dependent variables were classified as physical, cell differentials, …

Effect Of Light Intensity On Production Parameters And Feeding Behavior Of Broilers, Maurice Raccoursier Frost

Graduate Theses and Dissertations

This project was performed in two parts. The first was focused on light intensity as it affects performance. A randomized complete block design (RCBD) was performed. Broilers, Cobb 500 (n = 1584) were housed in 3 commercial houses (121.9 x 12.2 m). In each house birds were randomized and placed in 72 pens of 121.9 x 121.9 cm (22 bird/pen, males and females). All the treatment groups were provided with 24h light (L) during the first week and then 18L:6Dark (D) and 20 lux from day 7 to 14. The 3 intensity treatments of 5 lux (lx), 10 lx and …

Evaluation Of Long-Acting Eprinomectin Compared To Conventional Anthelmintics In Cow/Calf Production, Elizabeth Ann Backes

Graduate Theses and Dissertations

Experiment 1, 83, newly weaned, fall-born crossbred heifer calves were allocated randomly to 1 of 3 anthelmintic treatments: 1) control (CON); 2) combination pour-on moxidectin and oxfendazole (MO); and 3) long-acting eprinomectin (LAE). Two preplanned orthogonal contrast statements were used: 1) to compare CON to treated cattle; and 2) to compare OXF to LAE. Heifer BW and BCS were greater (P≤0.02) from MO and LAE on d 112, 140, 154, 168, 182 compared to CON. Heifer cyclicity, estrous detection, natural service and overall pregnancy rates were greater (P≤0.02) for MO and LAE compared to CON. Cattle fecal egg counts (FEC) …

Proteomic Characterization Of Eggshell Membranes And Their Effect On Poultry Physiology And Immunity, Sarbjeet Kaur Makkar

Graduate Theses and Dissertations

The use of antibiotics in poultry growth and disease control has led to antibiotics resistant problem in human beings, which is a big concern among consumers. With the necessity for judicious use of antibiotics in poultry production, alternative strategies to improve disease resistance in poultry production are necessary. The research is more inclined towards using the natural products available to grow healthier and antibiotic free meat animals. In the context of exploring natural and sustainable resource of alternative to antibiotics, the biochemical milieu of eggshell membranes (ESM) were analyzed by using mass spectrometry techniques including matrix assisted laser desorption ionization …

Evaluation And Selection Of A Bacillus Based Direct-Fed Microbial Candidate For In Situ Enzyme Production To Improve Gut Health Integrity, Bone Quality And Growth Performance In Poultry, Juan David Latorre Cardenas

Graduate Theses and Dissertations

During the last decade, the increasing interest in renewable energy sources has been changing the distribution of corn utilization from human and animal consumption to biofuel production, leading to a continuous rise in feed costs of livestock diets. Therefore, alternative feed ingredients such as distillers dried grains with solubles (DDGS), as well as cereals like wheat, barley, and sorghum have become part of the feed matrix to maintain or reduce production costs. However, these raw materials often contain a higher concentration of antinutritional factors in comparison to corn, including non-starch polysaccharides (NSP) which increase digesta viscosity and reduce nutrient absorption …

Efficacy Of A Novel Intranasal Zinc Solution On The Microbiome, Health, And Growth Performance Of High-Risk, Newly Received Stocker Cattle, Makenzie Foster

Graduate Theses and Dissertations

The objective of this study was to determine if using an intranasal zinc (Zn) solution would impact health and growth performance of high-risk stocker cattle. Male beef calves (n = 239; 3 arrival dates [block]; initial BW = 276 ± 2.4 kg) were stratified by arrival gender and BW and assigned to 1 of 2 treatments: 1) treated with 3 ml of a Zn solution containing 36.24 mg of Zn administered intranasally, or 2) control, in which calves were not treated. Calves were observed daily and if exhibiting signs of morbidity and a rectal temperature ≥ 40° C they were …

The Abundance And Distribution Of Mallards In The Lower Mississippi Alluvial Valley Of Arkansas, John Andrew Herbert

Graduate Theses and Dissertations

The management of wintering waterfowl in North America requires flexibility because of constantly changing landscapes and conditions. Many mallards use the lower Mississippi Alluvial Valley (MAV) for wintering habitat, making this an area of emphasis for improving management strategies. In this study, I used mallard observation data from 2009-2014 aerial surveys collected in the Arkansas portion of the lower MAV to explain the abundance and distribution and of mallards. Using spatial hierarchical models and breaking covariate data to 2x2 km grid cells, I analyzed how covariates relate to the changes of abundance and distributions within and among surveys. Mallard abundance …

Responses Of Two Strains Of Broilers To Diets Varying In Essential Amino Acid Density And Energy Levels, Tyler James Clark

Graduate Theses and Dissertations

The responses of two different commercial strains of broilers to diets varying in overall amino acid levels and metabolizable energy levels were observed. Amino acid levels used were 80%, 90%, 100%, 110%, 120%, and 130% of Ross recommended minimum digestable levels of essential amino acids. Energy levels used were either below or above Ross recommended metabolizable energy levels. Diets were fed for 56 days. The two strains were a fast growing broiler strain and a slower growing broiler strain. A selection of birds from each pen was processed at 35, 43, and 56 days of age. Average body weights, cumulative …

In Vivo Periodical Monitoring Of Immune Cell Infiltration In Response To Feathers And Intramuscular Injection Of Ionps Using The Pulp (Dermis) Of Growing Feathers As Test Site Tissue In Chickens, Olfat Taleb Alaamri

Graduate Theses and Dissertations

The applications of nanoparticles are growing, but little is known about their interactions with the immune system as most studies did not use suitable in vivo test systems. The purpose of these studies was to investigate the utility of chicken growing feathers as an in vivo test site for iron oxide nanoparticle (IONP) interactions with the immune system. The first objective of this study was to monitor leukocyte infiltration into the growing feather pulp upon the administration of IONP and IO-mIgG preparations. The second objective was to test the utility of IONPs as vaccine adjuvants by monitoring primary and memory …

Grass Finishing Systems For Lambs, Erin Leigh Smyth

Graduate Theses and Dissertations

There is strong interest in sustainably produced meat. Grass-fed lamb could fulfill this market by reducing off-farm inputs. The objective was to examine the effect of grass-finishing or minimal supplementation on performance of lambs in the southeastern US. Katahdin lambs born in October 2013 and 2014 and February 2014 were weaned in January 2014/2015 and May 2014, respectively. Lambs were blocked by gender (fall; winter included only ram lambs) and randomly assigned to receive no (NON) or grain co-product supplement (SUP; 15% CP) at 0.5% of BW/d. Lambs were rotationally grazed on predominantly grass. Body weight, fecal egg counts (FEC), …

Effects Of Crystalline Amino Acid Supplementation Of Reduced Crude Protein (Rcp) Diet On Net Energy Basis On Growing-Finishing Swine, Daniel Cook

Graduate Theses and Dissertations

Barrows and gilts (n=210) were used to test the effects of crystalline amino acid (AA) supplementation of reduced crude protein (RCP) diets formulated on a net energy basis on quality characteristics of the LM from growing-finishing pigs. Pigs were blocked by weight, and pens (3 barrows and 3 gilts/pen) within each block were assigned randomly to either corn-soybean meal diets (C) or 1 of 3 RCP diets and added crystalline AA levels for the dietary treatments during each feeding phase. During the last 3-wk feeding phase, 10 ppm of ractopamine were included in all diets. At slaughter and after a …

Efficacy Of Beta-Resorcylic Acid To Reduce Campylobacter Jejuni In Pre-Harvest And Post-Harvest Poultry, Basanta Raj Wagle

Graduate Theses and Dissertations

Campylobacteriosis is one of the leading foodborne illnesses in United States, and is associated with the consumption of poultry and poultry products. Reducing Campylobacter in these species will reduce the burden of this disease. Unfortunately, most strategies employed to reduce Campylobacter in poultry have either not been successful or produced inconsistent results. One potential control strategy is the use of β-resorcylic acid (BR), a phytophenolic compound classified by the US FDA as "Everything Added to Food in the United States" (EAF 3045) and is therefore deemed safe for consumption. This compounds has antibacterial activity against Salmonella, however, its efficacy to …

Effects Of Biomass Harvest On Eastern Red-Backed Salamanders, Patrick J. Ruhl

Open Access Theses

In a typical forest harvest, the volume of coarse woody debris (CWD) increases from nonmerchantable material (i.e., tree-tops, limbs, and small-diameter trees) left on the forest floor. Biomass harvesting removes much of this material for bioenergy production. When removed, ecosystem services associated with CWD, such as seedbed substrate, nutrient cycling, and essential wildlife habitat, is reduced. Woodland salamanders have strict microhabitat and soil moisture requirements that make them especially sensitive to timber harvest practices, particularly those that remove CWD, a primary habitat for the group.

I monitored the abundance of Eastern red-backed salamanders ( Plethodon cinereus ) in response to …

Identification Of Biomarkers Associated With Ascites Incidence In Broilers, Kaylee Rowland

Graduate Theses and Dissertations

Poultry is key in genetic research due to breeding feasibility, relatively short generation interval, and distinct phenotypes. It is estimated that 8% of broiler deaths annually can be attributed to ascites, an economically important disease that has been challenging the industry for the past 2 decades. Genetically selected ascites resistant (RES) and susceptible (SUS) chicken lines have been established and maintained by the University of Arkansas, Fayetteville. Intensive research efforts have been made to reveal physiological and biochemical characteristics for the incidence of ascites. Since the whole genome of the major ancestral chicken, the Red Jungle Fowl, has been sequenced, …

Relationships Between Landscape Factors And Crayfish Density And The Interacting Effects Of Grazing And Increased Sedimentation On Algal Biomass, Katheryn Haley Rose

Graduate Theses and Dissertations

In recent years in the United States there has been a rapid expansion in anthropogenic sources of sediment in streams including construction, agriculture, and drilling for natural gas. Potential effects land disturbance associated with activities from natural gas development on aquatic biota in surrounding streams have not yet been well documented. An increase in inorganic sediment in streams can be detrimental to organisms through a variety of mechanisms including alteration of dominant substrate type, higher turbidity resulting in lower visibility, and burial of food resources such as algae and detritus. Increasing sedimentation in stream environments through anthropogenic disturbance is a …

Greater Sage-Grouse (Centrocercus Urophasianus) Habitat Selection In Northwestern Wyoming And Stable Isotope Analysis Of Fecal Material, Andrea Renee Green

Graduate Theses and Dissertations

Dramatic range-wide declines in Greater Sage-Grouse populations have prompted efforts to determine habitat characteristics that are selected by sage-grouse for foraging, nesting and brood-rearing areas in an effort to conserve this species. Managers at Heart Mountain and Y U Bench in northwestern Wyoming expressed the need to quantify various habitat characteristics and to determine key use areas at both study sites. Data were collected on a variety of habitat variables in spots selected by grouse for foraging, nesting, and brood-rearing activities. These variables were compared to the same variables measured at random points at both study sites. Significant differences existed …

Turpentine Creek: Arkansas' Refuge, Jacob Paul Bain

Graduate Theses and Dissertations

The goal of this Master's thesis film project was twofold. First, I wanted to explore the problem, huge but relatively unknown, of the ownership of exotic pets in the United States of America. Second, in conjunction with that problem, I wanted to see how Turpentine Creek Wildlife Refuge (TCWR) approached it and what, as an organization, they do to help resolve this problem locally and nationally. In turn, the film also provides an in-depth look at a very unique refuge in the state of Arkansas. Using my own equipment, I shot and edited a short-form documentary about TCWR. The film …

The Interactive Effects Of Multiple Stressors On Lithobates Catesbeianus And Anaxyrus Americanus, Matthew Kyle Holden

Graduate Theses and Dissertations

Amphibian populations worldwide have experienced dramatic declines, and many species have already become locally, regionally, or globally extirpated with thousands more being threatened with extinction. These declines have occurred more rapidly in amphibians than any other group of vertebrates, which is especially concerning to scientists because amphibians serve as indicator species of overall environmental health. Major causes for amphibian declines are discussed in Chapter 1 and include: habitat modification and destruction, commercial over-exploitation, introduced species, environmental contaminants, global climate change, and infectious diseases.

Chapter 2 discusses the major research aspects of the thesis by examining the interactive effects of multiple …

Evaluation Of Water Sanitation Options For Poultry Production, Pramir Maharjan

Graduate Theses and Dissertations

An evaluation of poultry farm water supplies was conducted to determine the value and impact of water system sanitation practices in commercial broiler houses on microbial levels. Water line cleaning between flocks using concentrated disinfectant solution before placing chicks reduced biofilms retained in the lines to a safe level. Occasional microbial surges were noticed during different points of flock grow-out period even when daily water sanitation was present indicating water is highly susceptible to microbial contamination. However, the daily water sanitation practice controlled the occasional microbial surges in water from sustaining and kept drinking water to a microbiologically acceptable level. …

Ecology And Structure Of Black Bear (Ursus Americanus) Populations In The Interior Highlands Of Arkansas, Thea Vandervelde Kristensen

Graduate Theses and Dissertations

In the Interior Highlands of Arkansas, Missouri, and Oklahoma, over-harvest, extensive logging, and reductions of habitat availability by other means contributed to the decline of black bears (Ursus americanus). Bears were extirpated from the majority of the region by the 1940's Oklahoma by 1915 and from Missouri by 1931. From 1958-1968, the Arkansas Game and Fish Commission undertook a reintroduction to the Ouachita and the Ozark National Forests in Arkansas. The successful growth and expansion of the released population caused these efforts to be considered one of the most successful reintroductions of carnivores. In this dissertation, I sought to examine …

Effects Of Using Canola Meal As A Protein Source In Broiler Diets, Charles Danny Bradley Jr

Graduate Theses and Dissertations

Six experiments were conducted to evaluate the response of broilers fed canola meal. Experiment 1 assessed performance and carcass yields when broilers were fed various combinations of canola and soybean meal in nutritionally balanced diets based on digestible amino acid values. The results suggested that canola meal can be used in isocaloric diets as a partial replacement for Soybean meal.

Experiment 2 assessed broiler performance and carcass yields when using various levels of canola meal in broiler diets with a constant level of supplemental poultry oil. The resulting data suggested when diets are formulated with a constant level of supplemental …

Genetic Structure Of The Copperhead (Viperidae: Agkistrodon Contortrix Mokasen) At Its Most Northern Distribution, Brenna Aaren Levine

Graduate Theses and Dissertations

The focus of molecular studies in North American pitvipers has been on species that warrant conservation concern, such as Timber (Crotalus horridus) and Massasauga rattlesnakes (Sistrurus catenatus). Scant effort has been directed towards the molecular ecology of the Copperhead (Agkistrodon contortrix), other than to evaluate male reproduction in a laboratory setting and phylogenetic history. Thus, employing DNA-based molecular techniques to study the natural history of A. contortrix will broaden our knowledge of molecular ecology in North American pitvipers, and particularly in a non-threatened species that can serve as a surrogate for threatened and endangered …

Effects Of Ergot Alkaloids And Bovine Bodily Fluids On Cytochrome P450 Activity, Nicholas Scott Ezell

Graduate Theses and Dissertations

This thesis evaluates the Promega^TM P450-Glo assay (Promega^TM V9800) as a tool for quantifying ergot alkaloid concentration. Current techniques used for detection of ergot alkaloids are slow and expensive, do not detect all ergot alkaloids, or are not effective on bovine bodily fluids. The first study was conducted to determine effects of commercial ergot alkaloids (n = 6; 0 - 400 μM) on the Promega^TM P450-Glo assay. Cytochrome P450 (CYP450) activity in assay had a differential response to each ergot alkaloid and concentration. As concentrations of ergotamine, dihydroergotamine, ergocornine, and ergocryptine increased CYP450 activity was inhibited (P …

Genetic Analysis Of The Promoter Region Of The Serotonin Receptor 5ht2b And Its Contribution To Pulmonary Hypertension Syndrome In Broiler-Type Chickens, Khaloud Awada Alzahrani

Graduate Theses and Dissertations

Pulmonary Arterial Hypertension (PAH) is a sever disorder characterized by high pulmonary artery pressure and pulmonary vascular resistance along with specific histological changes. Idiopathic PAH is a human disease of unknown origin that showed common characteristics to PHS/ascites in chicken. PHS/ascites is a condition found in broilers causing right ventricular hypertrophy, valvular insufficiency, increased venous pressure, variable liver changes, accumulation of fluids in the abdominal cavity, and finally lung and heart failure. Currently, there is no acceptable animal model for human PAH. Our group has been validating the chicken as the medical animal model for human PAH, thus we have …

The Impact Of Bovine Spongiform Encephalopathy (Bse) On World Protein Supply, Jarrod Kersey

Graduate Theses and Dissertations

As world population, per capita income and urbanization increase, consumption of meat, poultry, milk and eggs will continue to rise to meet the needs of approximately nine billion people in 2050. Global beef, pig and chicken production and per capita consumption increased between 2000 and 2010, with growth in broiler production and consumption outpacing both beef and swine. The increased production and consumption requires readily available feed ingredients in regions where animal production is rapidly developing. Animal-based protein feed ingredients are often difficult to move from country to country due to real or perceived risk of animal disease. Zoosanitary standards …

Response Of Broiler Chickens To Variation In Dietary Nutrient Content, Sarah Diane Goodgame

Graduate Theses and Dissertations

Rising feed costs, environmental pollution concerns targeted at animal agriculture, a worldwide focus on sustainability and the never ending battle to improve production and efficiency within the poultry industry challenges nutritionists to focus on products to improve nutrient digestibility and decrease feed costs while at the same time maximizing performance. The research presented in this dissertation focuses on improving nutrient utilization of protein sources with protein liberating enzymes currently available commercially. The research also conducted addresses the addition of crystalline amino acids valine and isoleucine and their effect on improving efficiency of broilers.

The first research project compared the efficacy …

Habitat Modeling Of Three Endemic Crayfish Species In The Black River Drainage Of Missouri And Arkansas: Factors Affecting Distribution And Abundance, Matthew Stephen Nolen

Graduate Theses and Dissertations

The Ozark faunal region of Missouri and Arkansas harbors a high level of aquatic biological diversity, especially in regards to endemic crayfish. Orconectes eupunctus, Orconectes marchandi, and Cambarus hubbsi are three such endemics that are threatened by a limited natural distribution and the invasions of Orconectes neglectus. I sought to determine how natural and anthropogenic factors influence these three species across multiple spatial scales. Local and landscape data were used in decision tree analyses (CART) to determine their influence effect on presence/absence and density of the three species. Predictive models were validated using k-fold cross validation. O. eupunctus presence was …

Maternal Environment And Fescue Cultivar Effects On Growth, Development, And Fertility Of Beef Heifers, Jennifer Denyse Patterson

Graduate Theses and Dissertations

Eighty Brahman-influenced cows (5.6 BCS) were assigned to graze common bermudagrass (CB) or toxic tall fescue (E+) during a 60-d breeding season. Body condition (BC) was assessed at d 0, 30, and 60 of breeding season. Cows were managed to achieve marginal (4.2 BCS) or good (6.3 BCS) BC during last trimester. Heifer birth and weaning (WW; 7 to 8 mo) weights were recorded. The Angus sired heifers were weighed and randomly assigned to E+ or non-toxic (Novel) tall fescue at 9 to 10 mo of age for 190d. Heifer weight, growth data, and antral follicle count (AFC) were collected …

Evaluation Of Internal Markers For Predicting Digestibility And Fecal Output By Cattle Fed Bermudagrass Hays Of Varying Quality, Juvenal Kanani

Graduate Theses and Dissertations

The potential of in situ rumen undegradable dry matter (RUDM), indigestible neutral-detergent fiber (INDF), indigestible acid-detergent fiber (IADF), acid-detergent insoluble ash (ADIA), alkaline-peroxide lignin (APL), and acid-detergent lignin (ADL) to predict digestibility (DMD) and fecal output (FO) by cattle fed bermudagrass [Cynodon dactylon (L.) Pers.] hay-diets categorized by their low (L), medium low (ML), medium high (MH), or high (H) CP concentrations (79, 111, 131, and 164 g/kg DM, respectively) was evaluated. The second objective was to evaluate the effects of time (0600, 1200, 1800, and 2400 h) of fecal sampling on the prediction of FO and DMD. A replicated …

Comparative Response Of Different Broiler Genotypes To Dietary Nutrient Levels, Franco Javier Mussini

Graduate Theses and Dissertations

ABSTRACT

Three studies were conducted to evaluate how the broiler has changed over the years due to intense genetic selection. Four different broiler genotypes, one unselected since the 1950's (HER) representing the old meat-type bird and three current strains (Ross 308, Ross 708, and a test product (TY)) were studied. Differences in growth, morphometry, and response in performance and processing yield to different nutrient levels were studied.

Experiment 1 evaluated growth and morphometric analysis. Birds of each strain were killed weekly from day 7 to day 56, necropsied and cut up parts and organs weighed. Measurement of small intestine segments …