Life Sciences Commons^™

Application Of Near-Infrared Spectroscopy And Chemometrics For The Rapid Detection Of Insect Protein Adulteration From A Simulated Matrix, Dongdong Ni, Joost L. D. Nelis, Amanda L. Dawson, Nicholas Bourne, Pablo Juliano, Michelle L. Colgrave, Angéla Juhász, Utpal Bose

Research outputs 2022 to 2026

The popularity of insect protein as a food and feed supplement is growing. Protein quality, end use and prices vary considerably between different insect species, which may incentivise insect protein adulteration. Here, near-infrared (NIR) spectroscopy and chemometrics were used to detect the presence of cricket, black soldier fly larvae (BSFL) and mealworm proteins in a simulated complex insect protein mixture. Additionally, BSFL protein powders collected from three commercial sources were investigated to determine whether the NIR-based technology can discriminate the proteins obtained from different companies based on their composition. The proximate analysis suggests compositional protein, fat and chitin differences between …

Development Of An Enhanced Sampling Workflow To Accelerate Molecular Docking With Sparse Biophysical Information, Zachary Stichter

Masters Theses & Specialist Projects

Rapid docking of flexible biological macromolecules remains a significant open challenge in protein structure determination. While rigid docking is relatively simple with toolkits such as TagDock, a key obstacle to rapid flexible docking is the complexity and roughness of the free energy surface associated with protein conformational motion (often termed the many-minima problem), meaning conventional molecular dynamics methods do not effectively sample protein conformations near the interaction complex in accessible timescales. Methods such as metadynamics and replica exchange molecular dynamics exist to ameliorate this obstacle, yet these methods use nonphysical biases or random swaps to enhance sampling. In contrast, high …

The Effect Of Mindfulness Training On Muscular Endurance Exercise Performance, Mindfulness, And Mental Toughness, Donte Mcgee

Masters Theses & Specialist Projects

INTRODUCTION: Mindfulness training is a method of mental training becoming increasingly popular and has been positively associated with mental toughness, which may be valuable in highly fatiguing and stressful activities, like endurance exercise. Mindfulness training has resulted in increased endurance performance, but limited literature exists on a similar modality, muscular endurance exercise. The purpose of this study is to examine the effect of an acute bout of mindfulness meditation on muscular endurance performance, mindfulness, and mental toughness. METHODS: Participants (n=24) completed two sessions, a week apart, of three planks until fatigue, with one-minute rest intervals. Immediately following each plank, RPE …

Proposing Urban Agroforestry Designs For Lincoln, Nebraska: A Model From Berlin, Germany, Noah Johnson

Honors Theses

Given the threat of a worsening climate crisis, there is a strong need for community and ecosystem resilience. Diverse urban agroforestry systems have the potential to accomplish both and meet many of the objectives outlined in the city of Lincoln, Nebraska’s climate action plan. Additionally, Berlin, Germany could provide an effective model for Lincoln in this regard given the city’s extensive history of established urban agroforestry systems. The objective of this study then is to develop a design for an urban agroforestry site modeled on Berlin’s allotment gardens and tailored to Lincoln’s needs. The methods for creating this design included …

Determining The Impacts Of Corn Silage Mixing Ratios On Hydrogen Production From Beef Cattle Manure, Katya Faber-Quimby

Honors Theses

With global warming becoming an increasingly serious threat and a growing population requiring additional energy resources, interest in the production of clean energy from the agricultural sector has surged in popularity. Biohydrogen, also known as green hydrogen, is H₂ that has been produced using renewable energy, such as from agricultural byproducts or waste materials. It presents a promising alternative to nonrenewable resources due to its high potential for energy storage and lack of carbon emissions when used. Cattle manure has been explored as a resource for the production of biohydrogen. Selecting for hydrogen-producing microbes in the manure serves the …

Exploring The Impact Of Subject Placement On Exam Questions​, Briana M. Craig, Jeremy L. Hsu

Student Scholar Symposium Abstracts and Posters

Quizzes and exams are crucial elements of undergraduate biology courses; however, little research has been done exploring the importance of the phrasing used in the questions on these assessments. Question framing has the potential to impact student performance and sense of belonging greatly. In this study, we conducted an experiment where we created three versions of the exact same question except for the framing for the experimental scenarios. One version was written with self-referential framing using the term “you,” simulating the student conducting the experiment; another version used classmate-referential framing, placing one of their peers in the experiment; the third …

Creating Sustainable School And Home Gardens: Vertical Gardening, Tyelar Haines, Rita Hagevik, Kathy Cabe Trundle

All Current Publications

This fact sheet is part of a series about creating sustainable school and home gardens. It provides information and resources on vertical gardening, which can maximize space, increase food production, and improve plant health.

Psychology Of Athletes: Impact Of Injuries On Mental Health, Caitlin Rainey

Whittier Scholars Program

This article aims to contextualize the importance of understanding injuries in athletics and how those injuries impact mental health. With such a high prevalence of injuries in athletics, most studies analyze the physical implications on the athlete. It is vital that mental well being is studied as much as physical health throughout the injury and rehabilitation process. This study reviewed previous literature on the social impacts of team sports, psychological and social implications of injury, the impact of residual pain, and finally the process of retirement. A survey was also conducted at a small liberal arts college in Southern California …

Comparative Accumulation And Effects Of Microplastics And Microplastic-Associated Pcb-153 In The White Hard Clam (Meretrix Lyrata) And Giant River Prawn (Macrobrachium Rosenbergii) Following Chronic Exposure, Bao-Son Trinh, Lien Thi Le, Loc Minh Tran, Gunther Rosen, Robert C. Hale

VIMS Articles

Global environmental abundance of microplastics (MPs) is increasing. MPs may sorb hydrophobic organic contaminants (HOCs), accumulate in and cause deleterious effects on exposed organisms. This study investigated and compared the accumulation and effects of MPs and MP-associated PCB in the two indigenous aquatic organisms in Viet Nam, the white hard clams, Meretrix lyrata, and the giant river prawns, Macrobrachium rosenbergii. The test organisms were exposed to either polyethylene microbeads (PEMBs), waterborne polychlorinated biphenyl 153 (PCB-153), or PEMB-associated PCB-153 (PEMB-PCB) over 28 days. Organismal MP accumulation, survival, and weight gain were examined at various sampling intervals. In general, MP …

Virus Del Marchitamiento Manchado Del Tomate En Tomates Y Pimientos, Claudia Nischwitz, Mariah Noorlander, Mary Ann Hubbell, Celina Wille

All Current Publications

En Utah, el virus del marchitamiento manchado del tomate (TSWV, por sus siglas en inglés) comúnmente afecta a tomates, pimientos y tomatillos.

The Future Of The Organization Of The Petroleum Exporting Countries, Mikayla Gross

Honors Theses

The Organization of the Petroleum Exporting Countries (OPEC) is a multinational and intergovernmental organization that dictates the petroleum exportation policies of 13 countries: Algeria, Angola, Equatorial Guinea, Gabon, Iran, Iraq, Kuwait, Libya, Nigeria, the Republic of the Congo, Saudi Arabia, the United Arab Emirates and Venezuela (About Us, 2023). The mission of OPEC is to ensure a stable supply of petroleum through its shared policies for its Member Countries and consumers (Our Mission, 2023). Iran, Iraq, Kuwait, Saudi Arabia, and Venezuela founded OPEC in 1960 under the foundations and principles that dictated the international world order. Those foundations and principles …

Synthesis Of Gold Nanoparticles Via Pulsed Liquid Ablation For Use In The Photodynamic Therapy Of Bacteria, Justice Ben Yosef

Masters Theses & Specialist Projects

With the ever-increasing threat of antibiotic resistant bacteria, alternative treatment methods have been developed including photodynamic therapy (PDT). Within the PDT process, photosensitizers are used to generate reactive oxygen species (ROS) and facilitate the cell termination process. This work lays the conceptual foundation for the functionalization of the photosensitizer methylene blue (MB) with gold nanoparticles (AuNPs) and INF-55 as a potential inhibitor of the AcrAB-TolC efflux pump to enhance the effectivity of the PDT process.

AuNPs were synthesized using pulsed laser ablation in an aqueous citrate solution. Both nanosecond and picosecond pulse durations, as well as both 532 nm and …

Acl Bracing: Is It Still Being Done Clinically And Does It Influence Confidence And/Or Biomechanical Performance During A Drop Vertical Jump?, Bailey Watson

Masters Theses & Specialist Projects

Introduction: Anterior cruciate ligament (ACL) ruptures account for 60% of sports-related injuries and the gold standard for treatment is surgical intervention to replace the damaged ligament to restore function of the ACL. Braces are typically used to return to previous physical activity levels; however, there are no clear guidelines on their utilization. Beyond the physical consequences of undergoing ACL reconstruction surgery, the psychological component is often ignored.

Purpose: The aim of this study is to better understand the impact of knee bracing on both biomechanical factors and confidence following ACL reconstruction. Two aims comprise the overarching purpose; the first is …

Cannabidiol (Cbd) And Resistance Training: Does Cbd Attenuate Acute Pain?, Guillermo Pacheco

Masters Theses & Specialist Projects

INTRODUCTION: Resistance training (RT) is an advantageous form of physical activity for health and performance benefits; these benefits are maximized when done strenuously. However, strenuous RT can induce acute inflammatory pain which can lead to delayed-onset muscle soreness (DOMS). Common analgesics like non-steroidal anti-inflammatory drugs (NSAIDs) can block protein synthesis, so other alternatives are needed for exercise and athletic communities to maintain physiological adaptations. Cannabidiol (CBD) is a non-psychotropic cannabinoid with purported anti-inflammatory, anti-oxidative, and analgesic properties. One of the main reasons for the use of CBD in exercise is pain alleviation; however, research in humans is limited. Therefore, the …

Grey Relational Analysis And Canonical Correlation Analysis Of Air Pollution In Three Kentucky Counties, Sarah Hartman

Masters Theses & Specialist Projects

Air pollution is a crucial factor that affects both the environment and public health. Various methods are available for assessing air quality and pollution levels, such as regression models, principal component analysis, and factor analysis tools. However, some of these methods present issues in multicollinearity and the nature of collected data. It is important to recognize that air pollution data is often uncertain, incomplete, and contains limited valid data points. Weather conditions and economic activities are also factors that can affect air pollution. With growing communities in Kentucky (KY), it is essential to address these factors as the state has …

Analyzing Phototaxis And Related Visual Behaviors Among Diverse Species Of Drosophila, Madeline M. Hill

Honors Theses

Phototaxis and related visual behaviors can vary between species, and thus members of the genus Drosophila make an excellent study system to examine the evolution of vision. While some existing research points to these phototactic behaviors arising due to mating requirements or due to their species-specific ecology or environmental factors, there exists a lack of understanding as to why striking behavioral differences can exist between closely related species, or between members belonging to the same genus. The present research seeks to uncover the specifics regarding these discrepancies in visual evolution and aims to provide a foundation of knowledge about visual …

Toxicological Effects And Treatment Of Accidental Ingestion Of Simparica Trio® In Felis Catus: An Investigative Review, Aussia Stander

Honors Theses

Simparica TRIO^®, a canine endectocide manufactured by Zoetis, contains sarolaner, moxidectin, and pyrantel pamoate salt in an orally administered chewable tablet. These compounds are all toxic to both cats and dogs in sufficient amounts. As Simparica TRIO^® rises in popularity among pet owners, Felis catus (felines, cats) in multi-pet households (households with both cats and dogs) are at increased risk of overdosage and toxicosis from accidental ingestion of Simparica TRIO^® due to their smaller weight.

Simparica TRIO^® is not for feline use and little literature exists on the symptoms of and treatment for accidental ingestion …

Sarcopenia And The Importance Of Resistance Training And Protein-Rich Diets For Prevention Of Muscle Loss In Older Adults: A Literature Review And Informational Pamphlet, Madison Steele

Honors Theses

The growing prevalence of sarcopenia among older adults as well as youths has prompted further research aimed at developing effective preventative measures and treatment plans. Sarcopenia is often thought to occur solely due to aging, but several other factors contribute to progressive muscle loss. Previous research studies have found an effect of physical activity and dietary protein levels on the risk of developing sarcopenia as well as its onset age. This study aims to review existing knowledge in the field to compile an extensive list of sarcopenia causes and methods of prevention. The main causes identified in this review are …

Investigating Large Repeats In The Arabidopsis Thaliana Mitochondrial Genome Using Mitotalens, Megan Gusso

Honors Theses

This lab project explores the complexity of the mitochondrial genome by investigating large genomic repeats and their effect on flowering plants’ reproduction and viability. Mitochondria are the site of cellular respiration to create energy and contain their own DNA. This is counterintuitive because the byproducts of cellular respiration impose a threat of mutation for the stored DNA. The mitochondrial genome differs in size between animals and plants. Plant mitochondrial DNA is larger due to the presence of large, noncoding repeated sequences, and a large amount of DNA of unknown function. These sequences undergo high levels of recombination and rearrangement. The …

A Review Of Rheb Activation Of Mtorc1 And The Great Mystery Of One Missing Gef, Jack Gregory

Senior Honors Theses

The mTORC1 pathway is involved in the regulation of cell growth and translation. The pathway has a complex web of activators and inhibitors to activate mTORC1. mTORC1 is regulated via a small GTPase called Rheb, which interacts directly with mTORC1. This GTPase and its GTPase activating protein (GAP), TSC1/2, have been widely studied to understand how the variety of regulators of mTORC1 interact with these proteins. Despite this, the guanine nucleotide exchange factor (GEF) of Rheb has yet to be identified. This review broadly analyzes Rheb and mTORC1, their structures, regulations, and interactions, and explores the mystery of the missing …

The Effects Of Water Submersion On Skeletal Muscle Activation, Fatigue, And Whole-Body Gas Exchange, Caroline Griffin, Robert J. Shute

Honors Projects

THE EFFECTS OF WATER SUBMERSION ON SKELETAL MUSCLE ACTIVATION, FATIGUE, AND WHOLE-BODY GAS EXCHANGE

C.C. Griffin. Bridgewater College, Bridgewater, VA.

Email: Cgriffin@eagles.bridgewater.edu

Fatigue of skeletal muscle can be affected by multiple environmental elements. Fatigue threshold can be measured by VO2 max on a cycle ergometer combined with the use of a metabolic cart and Electromyography (EMG) measurements. PURPOSE: The purpose of this experiment is to determine if water submersion affects skeletal muscle activation, fatigue, and whole-body gas exchange. METHODS: Three participants were recruited to participate in a cold and no temperature trial. Participants arrived and baseline vitals and measurements were …

Mast Cells In Mammary Carcinoma, Alexandria M. Ashbaugh

Undergraduate Honors Theses

Breast cancer (BC) is an aggressive disease that takes the lives of thousands of women every year. Although knowledge and treatment of this disease have improved over the years, much is still to be discovered about BC. One area of expertise that we are currently trying to learn more information about is the mast cell (MC) and its role in BC growth and development. The MC is an immune system component discovered in tumors' extracellular matrix (ECM). As a tumor grows within body tissues, the tumor recruits the MC from surrounding connective tissues to the tumor, using signaling cytokines such …

A Combination Of Generated Hydrogen Sulfide And Nitric Oxide Activity Has A Potentiated Protectant Effect Against Cisplatin Induced Nephrotoxicity, Faria Khurshid, Javeid Iqbal, Fiaz Ud Din Ahmad, Arslan Hussain Lodhi, Abdul Malik, Suhail Akhtar, Azmat Ali Khan, Marvi Imam Bux, Mohammed Younis

Biomedical Sciences Faculty Publications

Aim: Hydrogen sulfide and nitricoxide possess cytoprotective activity and in vivo, they are generated from exogenous sodium hydrosulfide and L-arginine respectively. Cisplatin is a major chemotherapeutic agent used to treat cancer and has a high incidence of nephrotoxicity as a side effect. The study aim was to explore the effects of NaHS and L-arginine or their combination on cisplatin induced nephrotoxicity in rats. Methods: Wistar Kyoto rats were given a single intraperitoneal dose of cisplatin (5 mg/kg) followed either by NaHS (56 μmol/kg, i. p.), L-arginine (1.25 g/L in drinking water) or their combination daily for 28-days. Post-mortem plasma, urine …

Exploring The Intersection Between West Virginia Farmland Protection Boards, Landowners, And The West Virginia Agrarian Commons, Samuel W. Bayne, Joshua Lohnes

Undergraduate Scholarship

New Roots Community Farm (NRCF) came to life through the purchase of a piece of land by the Fayette County Farmland Protection Board in a buy-protect-sell model. NRCF then partnered with The Agrarian Land Trust, a New England based organization experimenting with land commons models across the United States, to purchase the property and create the West Virginia Agrarian Commons (WVAC), an entity that could advance land access for the next generation farmers in WV. The experience of NRCF and the WVAC and the vision for its operation and extension of the buy-protect-sell model in partnership with county farmland protection …

Examining Eastern Food Hub's Food Access Programs: A Research Study Sustainable Development Internship (Sdi) Spring 2024, Devin Anthony Barkefelt, Fritz Boettner

Undergraduate Scholarship

In 2020, the US Department of Agriculture (USDA) introduced the Farmers to Families Food Box (FFFB) program. Its objectives were threefold: (1) deliver food to those in need, (2) support small and mid-sized producers, and (3) bolster food distribution companies through the pandemic. While the program ended in 2021, the USDA has continued to fund other programs, such as the Local Food Purchase Assistance Cooperative Agreement Program (LFPA) and the Local Food For Schools Cooperative Agreement Program (LFS). The WVU Center for Resilient Communities (CRC) is researched how previous, current, and future programs can be implemented to best support producers, …

Pathogenesis And Therapeutic Considerations For Nonalcoholic Fatty Liver Disease, Elijah Chung

Senior Honors Theses

A product of the Westernized, high-fat diet, non-alcoholic fatty liver disease (NAFLD) has emerged as the leading cause of chronic liver disease, affecting one-fourth of the world's population. NAFLD is a progressive disease arising from a multisystem response to excess lipids in the blood, adipose tissue, and liver. Despite the prevalence of NAFLD and its well-studied bidirectional association with obesity and type 2 diabetes mellitus and obesity, there is a shocking scarcity of available treatments aside from diet and lifestyle changes. Thus, further research on NAFLD and potential therapies is urgently needed. This paper will illustrate the major pathways associated …

The Alexander Technique Applied To Dance And The Choreographic Process: Freeing Physical Expression From Trauma-Based Tension, Julia Johnston

Senior Honors Theses

Ballet, contemporary, and modern dancers have expressed incurring trauma during their training and professional dance experiences; in a 2020 survey, 41% of professional dancers and 30% of ballet students reported experiencing or witnessing sexually inappropriate behavior in their respective workplaces and schools (DDP). This is just one example of a potential source of trauma for dancers. The physiological effects of trauma cause physical effects, creating tension in a dancer’s body. Dance relies on physical expression, the expression of thought and feeling through movement, to connect with the audience. Trauma-based tension inhibits a dancer’s range of physical expression and connection to …

An Examination Of The Eastern Nebraska And Western Iowa Flash Flood Event Of 6-7 August 1999, Catherine M. Zapotocny

NOAA Technical Reports and Related Materials

Flash flooding occurs each summer in eastern Nebraska and western Iowa. While 10-inch rains are extraordinary, it is not uncommon to have at least one excessive rain event during the annual convective season. A significant flash flood occurred in east central Nebraska and western Iowa the night of August 6, 1999 into the morning of August 7, 1999. Antecedent hydrologic conditions limited the loss of life, however considerable property damage resulted as the heavy rain fell over the urbanized areas in eastern Nebraska and western Iowa.

Several flash flood ingredients came together over the area. These meteorological factors are typical …

The Purification And Thermal Stability Of The Peroxidase Enzyme In Cucurbita Moschata, Garen Hamner

Senior Honors Theses

Peroxidases are enzymes that catalyze the reduction of hydrogen peroxide to water while oxidizing organic substrates and are valuable in spheres like industrial and medical applications and histochemistry. Limitations still exist in the use of the well-studied horseradish peroxidase for certain activities due to limitations like poor thermal stability, thus the search for novel peroxidases that can overcome these limitations is an active area of research. Butternut squash peroxidase (Cucurbita moschata) (BSP) shows promise due to significant activity being found in the skin and apparent enhanced thermal stability, but an efficient purification scheme for it is lacking, as well as …

Trna Anticodon Cleavage By Target-Activated Crispr-Cas13a Effector, Ishita Jain, Matvey Kolesnik, Konstantin Kuznedelov, Leonid Minakhin, Natalia Morozova, Anna Shiriaeva, Alexandr Kirillov, Sofia Medvedeva, Alexei Livenskyi, Laura Kazieva, Kira S Makarova, Eugene V Koonin, Sergei Borukhov, Konstantin Severinov, Ekaterina Semenova

Rowan-Virtua School of Osteopathic Medicine Faculty Scholarship

Type VI CRISPR-Cas systems are among the few CRISPR varieties that target exclusively RNA. The CRISPR RNA–guided, sequence-specific binding of target RNAs, such as phage transcripts, activates the type VI effector, Cas13. Once activated, Cas13 causes collateral RNA cleavage, which induces bacterial cell dormancy, thus protecting the host population from the phage spread. We show here that the principal form of collateral RNA degradation elicited by Leptotrichia shahii Cas13a expressed in Escherichia coli cells is the cleavage of anticodons in a subset of transfer RNAs (tRNAs) with uridine-rich anticodons. This tRNA cleavage is accompanied by inhibition of protein synthesis, thus …