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Articles 2851 - 2880 of 4510
Full-Text Articles in Life Sciences
Towards The Use Of Time-Resolved X-Ray Crystallography In Mechanistic Studies Of Cytochrome C Nitrite Reductase From Shewanella Oneidensis, Matthew David Youngblut
Towards The Use Of Time-Resolved X-Ray Crystallography In Mechanistic Studies Of Cytochrome C Nitrite Reductase From Shewanella Oneidensis, Matthew David Youngblut
Theses and Dissertations
A high-yield expression and purification of Shewanella oneidensis cytochrome c nitrite reductase (ccNiR), and its characterization by a variety of methods, notably Laue crystallography, is reported. A key component of the expression system is an artificial ccNiR gene in which the N-terminal signal peptide from the highly expressed S. oneidensis protein "Small Tetra-heme c" replaces the wild-type signal peptide. This gene, inserted into the plasmid pHSG298 and expressed in S. oneidensis TSP-C strain, generated approximately 20 mg crude ccNiR/L culture, compared with 0.5-1 mg/L for untransformed cells. Purified ccNiR exhibited nitrite and hydroxylamine reductase activities comparable to those of E. …
Investigation Of Web-Based Motivational Interviewing To Increase Physical Activity Participation Among Adults, Sasha Karnes
Investigation Of Web-Based Motivational Interviewing To Increase Physical Activity Participation Among Adults, Sasha Karnes
Theses and Dissertations
Interventions to enhance physical activity (PA) participation are needed given the high prevalence of under-activity and inactivity (CDC, 2001) and related occurrence of negative health consequences among the general adult population (Kung, Hoyert, Xu, & Murphy, 2008). Preliminary support for a therapeutic technique called Motivational Interviewing (MI) suggests promise for application to enhance PA participation (Burke, Arkowitz, & Menchola, 2003). Given the need for interventions to enhance PA and the preliminary support for MI as an intervention to increase PA, the aims of the current study were to: (a) determine if web-based MI is effective in enhancing PA participation, and …
High-Throughput Approaches For The Assessment Of Factors Influencing Bioavailability Of Small Molecules In Pre-Clinical Drug Development, Megan Marie Mccallum
High-Throughput Approaches For The Assessment Of Factors Influencing Bioavailability Of Small Molecules In Pre-Clinical Drug Development, Megan Marie Mccallum
Theses and Dissertations
A bioactive molecule must pass many hurdles to be designated as a "good" pharmaceutical lead or hit compound. It should have a significant activity, selectivity, bioavailability, and metabolic half-life. Many factors have been identified that influence the free drug concentration or bioavailability of orally administered drugs in the earliest development stages. In vitro pre-clinical assays have been developed to measure these parameters. The small molecule properties that are investigated here include aqueous solubility, permeability, reactivity (electrophilicity), small molecule-protein binding, and displacement of protein-bound molecules (drug-drug interactions). The development of rapid and miniaturized assays to quantify these factors is presented herein. …
Methods In Metallomics, Proteomics, And Toxicology: Development And Applications Of Laser Ablation Inductively Coupled Plasma Mass Spectrometry And Native Sds-Page, William John Wobig
Methods In Metallomics, Proteomics, And Toxicology: Development And Applications Of Laser Ablation Inductively Coupled Plasma Mass Spectrometry And Native Sds-Page, William John Wobig
Theses and Dissertations
Polyacrylamide gel electrophoresis (PAGE) is a bio-analytical method used to separate proteins in solution into an array of individual bands of proteins in a gel matrix. Current PAGE methods, however, have severe limitations in simultaneously maintaining a protein's native structure and association with transition metals while providing adequate resolution. Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) provides a means to perform trace to ultra-trace level inorganic analysis of solid samples such as dried PAGE gels containing metallo-protein arrays. Current LA-ICP-MS methods involving the analysis of PAGE gels, however, have been limited in their effective use by inadequate limits of …
Viral Hemorrhagic Septicemia Virus (Vhsv) Great Lakes Strain Ivb: Viral Detection, Mechanisms Of Infection, And Host-Virus Interactions In The Yellow Perch (Perca Flavescens), Wendy Joy Olson
Theses and Dissertations
Viral hemorrhagic septicemia virus (VHSV) is one of the most devastating and problematic viral fish diseases to plague the European aquaculture industry, and due to its pathogenicity, disease course, mortality rates, and wide host range, remains one of the most pathogenic viral diseases of finfish worldwide. A new freshwater strain of viral hemorrhagic septicemia virus IVb (VHSV-IVb) in the Great Lakes has been found capable of infecting a wide number of naive species, and has been associated with large fish kills in the Midwestern United States since its discovery in 2005. In this study, the yellow perch, Perca flavescence, one …
Analysis Of Genes Involved In Anaerobic Growth In Porphyromonas Gingivalis And Shewanella Oneidensis Mr-1, Dilini Sanjeevi Kumarasinghe
Analysis Of Genes Involved In Anaerobic Growth In Porphyromonas Gingivalis And Shewanella Oneidensis Mr-1, Dilini Sanjeevi Kumarasinghe
Theses and Dissertations
Porphyromonas gingivalis is an oral Gram-negative anaerobic bacterium implicated in periodontal disease, a polymicrobial inflammatory disease that is correlated with cardiovascular disease, diabetes and preterm birth. Therefore understanding the physiology and metabolism of P.gingivalis through genetic manipulation is important in identifying mechanisms to eliminate this pathogen. Although numerous genetic tools have been developed for the manipulation of other bacterial species, they either do not function in P.gingivalis or they have limitations. We modified a Mariner transposon pHimarEM1 system that was developed for Flavobacterium johnsoniae for mutagenesis of P. gingivalisWe introduced the P. gingivalis fimA promoter upstream of the transposase …
The Shifting Importance Of Competition And Facilitation Along Diversity, Environmental Severity, And Plant Ontogenetic Gradients, Alexandra Wright
The Shifting Importance Of Competition And Facilitation Along Diversity, Environmental Severity, And Plant Ontogenetic Gradients, Alexandra Wright
Theses and Dissertations
Ecological theory and empirical studies have focused heavily on the importance of competition in plant communities. Competition can help explain species coexistence, the maintenance of species diversity, and biological invasions. Competition for resources appears to be ubiquitous among coexisting organisms. This overwhelming focus on competition over the past one hundred years may have overshadowed the importance of positive interactions (facilitation). Growing near your neighbors involves competition for resources, but it also involves alteration of a shared microclimate. Neighboring plants have the capacity to increase shade, decrease air temperatures, increase humidity, and increase shallow soil moisture in their local environment. In …
A Network View On Neurodegenerative Disorders, Sreedevi Chandrasekaran
A Network View On Neurodegenerative Disorders, Sreedevi Chandrasekaran
Theses and Dissertations
Neurodegeneration is a chronic, progressive and debilitating condition that affects majority of the World's elderly population who are at greater risk. Numerous scientific studies suggest that there could be a common underlying molecular mechanism that promotes the degeneration and the subsequent neuronal loss, however so far the progress in this direction is rather limited. Abnormal protein misfoldings, as well as protein plaque formations in the brain, are some of the hallmark characteristic features of neurodegenerative disorders (NDDs). Genetic and environmental factors, oxidative stress, excessive reactive oxygen species formation, mitochondrial dysfunction, energy depletion and autophagy disruption etc. are some of the …
Phylometagenomics: A New Framework For Uncovering Microbial Community Diversity, Christopher J. Friedline
Phylometagenomics: A New Framework For Uncovering Microbial Community Diversity, Christopher J. Friedline
Theses and Dissertations
Microbial communities are recognized as major drivers of global biogeochemical processes. However, the genetic diversity and composition, as well as processes leading to the origin and diversification of these communities in space and time, are poorly understood. Character- ization of microbial communities using high-throughput sequencing of 16S tags shows that Operational Taxonomic Unit (OTU) abundances can be approximated by a gamma distribu- tion, which suggests structuring around small numbers of highly abundant OTUs and a large proportion of rare OTUs. The current methods used to characterize how communities are structured rely on multivariate statistics, which operate on pair-wise distance matrices. …
Occupational Athletes: An Integrated Approach To Firefighting Performance, Stacy L. Gnacinski
Occupational Athletes: An Integrated Approach To Firefighting Performance, Stacy L. Gnacinski
Theses and Dissertations
Introduction: Over the past 20 years, the injury rates among firefighters have captured the interest of sport scientists. In order to prevent firefighter injuries, however, scholars must first gain a better understanding of firefighting performance (Smith, 2011). This has been a challenge, since to date sport scientists have focused primarily on the physical aspects of firefighting performance and have overlooked the multidimensional nature of firefighting performance (Gnacinski, Meyer, & Ebersole, in press). In the sport arena, sport scientists often use theoretical models to conceptualize the multiple demands experienced by an athlete. Guided by an integrated model of sport performance, the …
The Effect Of Aerobic Fitness On Visuospatial Attention In Young Adults, Kelly Marie Kunowski
The Effect Of Aerobic Fitness On Visuospatial Attention In Young Adults, Kelly Marie Kunowski
Theses and Dissertations
The recently popular commercial brain- and visual-training programs have become a multimillion dollar industry with claims to enhance various cognitive functions. Although no empirical evidence directly supports the efficacy of these programs, sport expertise has been shown to influence cognition, lending indirect support for training efficacy. However, researchers investigating attention and sport expertise have not previously controlled for level of physical activity, which may also contribute to enhanced cognitive processes. Prior studies have shown strong correlations exist between physical fitness and cognition in both children and older adults. Yet, few studies have examined this relation in young adults, and no …
Prolonged Glucose Deprivation Sensitizes Snf1 To Negative Regulation By Pka To Delay Entry Into Quiescence, Leah Bernadette Doughty
Prolonged Glucose Deprivation Sensitizes Snf1 To Negative Regulation By Pka To Delay Entry Into Quiescence, Leah Bernadette Doughty
Theses and Dissertations
AMPK, the fuel gauge of the cell, and its upstream kinase, LKB1, have been implicated in cancer prevention and stress response associated with energy exhaustion. In the yeast Saccharomyces cerevisiae, Snf1 is the ortholog of mammalian AMPK. In S. cerevisiae, Snf1 is activated by phosphorylation of its T–loop at Thr210, primarily by its upstream kinase Sak1, in absence of the preferred carbon source, glucose, or during some other stress responses. Cyclic AMP–dependent protein kinase A, PKA, is involved in nutrient signaling largely antagonistically to Snf1. Using yeast strains of the Sigma 1278b genetic background, which have a high basal level …
Discrimination Trials To Influence Self-Awareness, Kerin A. Weingarten
Discrimination Trials To Influence Self-Awareness, Kerin A. Weingarten
Theses and Dissertations
Humans often use terms and concepts that include self- as prefix in an effort to explain their behavior (e.g., self-awareness, self-control) (Goldiamond, 1959, 1962, 1965, 1966; Nisbett & Wilson, 1977). Although there are many ways to interpret such terms, they all seem to involve circumstances in which individuals' own prior behavior is discriminative for their subsequent behavior. Behavior under the discriminative control of other behavior may be a kind of self-report (Skinner, 1957). The concepts of self-awareness and self-report can be studied as a behavioral process, without mentalistic inference. In the present research, pigeons were trained in a compound, discrete-trial …
Identification Of Immunomodulatory Cells Induced By 670 Nm Light Therapy In An Animal Model Of Multiple Sclerosis, Erin Christine Koester
Identification Of Immunomodulatory Cells Induced By 670 Nm Light Therapy In An Animal Model Of Multiple Sclerosis, Erin Christine Koester
Theses and Dissertations
Multiple sclerosis is an autoimmune, demyelinating disease characterized by neurodegeneration and inflammation of the central nervous system. It affects approximately 250,000 people in the United States alone, with women being affected two times more than men. Experimental Autoimmune Encephalomyelitis (EAE) is the primary animal model of MS, sharing clinical signs and histopathology with MS. The current paradigm supports MS/EAE induction by myelin reactive CD4+ T cells that cross the blood brain barrier to induce an inflammatory response that leads to the destruction of the myelin sheath and eventual loss of axons. Recent data suggest that axonal loss and disease progression …
Behavior Change In Applied Sport Psychology: The Use Of Processes Of Change In Psychological Training For Athletes, William Vincent Massey
Behavior Change In Applied Sport Psychology: The Use Of Processes Of Change In Psychological Training For Athletes, William Vincent Massey
Theses and Dissertations
The results of previous research (e.g. Leffingwell, Rider, & Williams, 2001; Massey, Meyer, & Hatch, 2011; Zizzi & Perna, 2003) have led scholars to conclude that the Transtheoretical Model (TTM) may be an appropriate paradigm to study readiness to change in sport psychology settings. However, processes of change - a critical element to the TTM - have yet to be studied or measured in an athlete population. As such, the purpose of the current investigation was to initially develop and examine a measure of the processes of change for use in applied sport psychology settings. Informed by relevant literature, an …
The Effect Of Exertion On Intra-Limb Joint Coordination Variability During Running Using A Waveform Analysis Approach, Lauren Benson
The Effect Of Exertion On Intra-Limb Joint Coordination Variability During Running Using A Waveform Analysis Approach, Lauren Benson
Theses and Dissertations
About half of all runners sustain a running-related injury in a given year. Less variable joint coordination patterns may be detrimental as stress endured by the same tissue, encountered over many running cycles, could lead to overuse running injuries. The effects of fatigue may contribute to runners' risk of injury by altering joint coordination variability. Since fatigue is task-dependent, it is practical to consider a level of fatigue typically experienced by runners. The purpose of this study was to examine the influence of running in an exerted state on lower extremity joint coordination variability, using Principal Components Analysis (PCA) and …
Analysis Of Contributing Factors To Obesity In Children And Adolescents In Southwest Georgia, Sydney L. Worthy
Analysis Of Contributing Factors To Obesity In Children And Adolescents In Southwest Georgia, Sydney L. Worthy
Theses and Dissertations
Today more than 23 million U.S. children and adolescents are either obese or overweight. Hospital costs from obesity-related diseases in youth have increased from $35 million (0.43% of total hospital costs) during 1979 to 1981 to $127 million (1.79% of total costs) during 1997 to 1999. During the Pathway to Med School program, I collaborated with three other students to develop a research project on childhood and adolescent obesity. We conducted surveys at nine different primary care clinics in the southwest Georgia area. Approval was sought by the Institutional Review Board of Phoebe Putney Memorial Health System and Albany Area …
Characterization Of Bioreducible Poly(B-Amino Ester) Nanoparticles For Sirna Delivery, Bolivia Hurtado De Mendoza
Characterization Of Bioreducible Poly(B-Amino Ester) Nanoparticles For Sirna Delivery, Bolivia Hurtado De Mendoza
Theses and Dissertations
Glioblastoma multiforme (GBM) is one of the most malignant brain tumors affecting adults. It is characterized by necrotic tissue and abnormal vasculature, making it highly resistant to current cancer treatments. A promising alternative to standard cancer therapeutics is the use of drug delivery systems such as polymeric nanoparticles (NP) that deliver silencing RNA (siRNA) exclusively to tumor cells for gene knockdown. Presently, nonviral delivery vectors, such as poly(B-amino ester) (PBAE) NP, are beneficial delivery systems because they are less immunogenic and easier to chemically modify than vectors delivered by viruses. Although current formulations of PBAEs allow cargo release via hydrolytic …
Bioinformatics Approach To Probe Protein-Protein Interactions: Understanding The Role Of Interfacial Solvent In The Binding Sites Of Protein-Protein Complexes;Network Based Predictions And Analysis Of Human Proteins That Play Critical Roles In Hiv Pathogenesis., Mesay Habtemariam
Theses and Dissertations
The thesis work contains two projects under the same umbrella. The first project is to provide a detailed analysis on the behavior of interfacial water molecules at protein-protein complexes, in this case focusing on homodimeric complexes, and to investigate their effect with respect to different residue types. For that reason the homodimeric data-set, which includes high-resolution (≤ 2.30 Å) X-ray crystal structures of 252 (140 Biological & 112 Non-biological) protein complexes was chosen to explore fundamental differences between interfaces that Nature has “engineered” vs. compared to interfaces found under man-made conditions. The data set was comprised of 5391 water molecules …
The Effects Of Tarsh Overexpression On Lung Carcinomas, Young Kim
The Effects Of Tarsh Overexpression On Lung Carcinomas, Young Kim
Theses and Dissertations
Lung cancer arises from epithelial cells that line the air passages of the lungs. It is the second most common malignancy in the United States; trends suggest that over 228,000 new patients will be diagnosed with lung cancer in 2013. Due to the fact that lung cancer is highly aggressive, it has proven difficult to control. The 5-year survival rate has been shown to be only 15.9%, despite the advances made in terms of diagnosis and treatment. Therefore, we are faced with the problem of finding more effective methods that allow for an earlier diagnosis and the improved treatment of …
The Role Of Camk-Ii In Skeletal Muscle Function And Swimming Behavior In Zebrafish, Minh Nguyen
The Role Of Camk-Ii In Skeletal Muscle Function And Swimming Behavior In Zebrafish, Minh Nguyen
Theses and Dissertations
Previous research showed mutations in muscle sarcoplasmic reticulum-bound calcium handler proteins cause swimming defects in embryonic zebrafish. CaMK-II is a highly conserved Ca2+/calmodulin-dependent protein kinase expressed in all vertebrates has been defined to activate and inactivate multiple Ca2+ handler proteins involved in excitation- contraction coupling and relaxation of cardiac and skeletal muscle. In this study, evidence is provided through pharmacological and genetic intervention that CaMK-II inhibition and overexpression causes swimming defects, particularly response to stimuli and swimming ability, reinforced by immunolocalization of skeletal muscle. Transient CaMK-II inactivation does not have any long-term defects to swimming behavior. Overexpression of wild-type, constitutively …
Structural And Functional Characterization Of Sortase A, Vishaka Santosh
Structural And Functional Characterization Of Sortase A, Vishaka Santosh
Theses and Dissertations
Sortases have been known to be essential in Gram-positive bacteria for attaching proteins onto the peptidoglycan layer of the bacterium. Sortase A has been found to be useful as a “molecular stapler”, although; in vivo, the enzyme is responsible for attaching proteins to the peptidoglycan layer of Gram-positive bacteria. It accomplishes both of these tasks by joining two proteins together via an LPXTG sorting sequence. The enzyme has been proven to be very useful in attaching any two proteins together without worrying about recombinant techniques to generate the fusion protein. The problem with this enzyme is that the catalytic diad, …
The Inflammasome In Acute Myocarditis, Harsha Kannan
The Inflammasome In Acute Myocarditis, Harsha Kannan
Theses and Dissertations
Acute myocarditis is an acute inflammatory syndrome characterized by myocardial damage and dysfunction often due to a viral infection followed by a variable development over time. There are currently no specific treatments and standard treatments for heart failure are generally applied. The inflammasome is a recently identified macromolecular structure that occupies a central role in the amplification of the inflammatory response and promotion of cell death during acute and chronic infections. We hypothesized the formation of the inflammasome in acute myocarditis. To investigate, samples of patients were collected from the Cardiomyopathy Registry in Trieste, with 12 cases of biopsy-proven myocarditis …
Camk-Ii: An Integral Protein In Cell Migration, Jamie Josephine Avila Mcleod
Camk-Ii: An Integral Protein In Cell Migration, Jamie Josephine Avila Mcleod
Theses and Dissertations
Coordinated inductive and morphogenetic processes of gastrulation establish the zebrafish body plan. Gastrulation includes massive cell rearrangements to generate the three germ layers and shape the embryonic body. Three modes of cell migration must occur during vertebrate gastrulation and include: epiboly, internalization of the presumptive mesendoderm and convergent extension (C&E). C&E movements narrow the germ layers mediolaterally (convergence) and elongate them anteroposteriorly (extension) to define the embryonic axis. The molecular mechanisms regulating coordinated cell migrations remain poorly understand and studying these has become of great interest to researchers. Understanding cell migration during development is highly relevant to a number of …
The Activation, Receptor Complexing And Endogenous Regulation Of The Type-I Interferon Response As It Pertains To Innate Immunity, James D. Marion Jr.
The Activation, Receptor Complexing And Endogenous Regulation Of The Type-I Interferon Response As It Pertains To Innate Immunity, James D. Marion Jr.
Theses and Dissertations
To defend against pathogen challenge, multi-cellular organisms mount an immune response that recognizes, sequesters and eradicates invading infectious agents. Critical to this safeguard is the receptor-mediated detection of pathogens. Pathogen recognition then initiates a variety of signaling cascades that lead to the modulation of genes orchestrating an immune response. Toll-like receptor 3 (TLR3), a transmembrane receptor found in endosomes, is vital to the innate immune response against viruses. Double-stranded RNA (dsRNA) stimulation of TLR3 initiates a signaling cascade that leads to the production of type-I interferons and proinflammatory cytokines necessary to trigger the protective defenses of the immune system. Critical …
Kinetics Of Atmospheric Reactions Of Biogenic Volatile Organic Compounds: Measurement Of The Rate Constant Ofthujone + Cl· At 296 K And Calculation Ofthe Equilibrium Constant For The Ho2ch2ch2o2· H2o Complex, Marie Coy Killian
Theses and Dissertations
Biogenic volatile organic compounds (VOCs) react with Cl and OH radicals and the resulting radicals combine with oxygen to form peroxy radicals RO2. Organic peroxy radicals can then react with NO to form NO2, a precursor of tropospheric ozone. The work presented here explored the initial reaction between Cl and thujone, a VOC emitted by Great Basin sagebrush. The rate constant for the reaction of thujone + Cl at 296 K was measured with the method of relative rates with FTIR for detection of reactants. LEDs were used to photolyze Cl2 to generate Cl in the reaction cell. Thujone was …
Characterization Of The Neurotrophic Factor Brain-Derived Neurotrophic Factor (Bdnf) In Intestinal Smooth Muscle Cells, Mohammad Alqudah
Characterization Of The Neurotrophic Factor Brain-Derived Neurotrophic Factor (Bdnf) In Intestinal Smooth Muscle Cells, Mohammad Alqudah
Theses and Dissertations
Brain-derived neurotrophic factor (BDNF) belongs to the neurotrophin family of secreted proteins, which include in addition to BDNF, nerve growth factor (NGF) and neurotrophin 3-6 (NT-3-6). BDNF mediates its functions by activating two cell surface receptors, pan-neurotrophin receptor (P75NTR) and tropomyosin-related kinase B (TrkB) and their downstream intracellular cascades. BDNF is best known for its role in neuronal survival, regulation of neuronal differentiation, migration and activity-dependent synaptic plasticity. However, BDNF is widely expressed in non-neuronal tissues as well. The localization and the function of BDNF in intestinal smooth muscle cells (SMCs) are not well defined. Thus, the main purpose of …
Sildenafil Attenuates Ethanol-Induced Cardiomyocyte Injury And Preserves Cardiac Function Through Protein Kinase G-Dependent Signaling, Gregory R. Sturz
Sildenafil Attenuates Ethanol-Induced Cardiomyocyte Injury And Preserves Cardiac Function Through Protein Kinase G-Dependent Signaling, Gregory R. Sturz
Theses and Dissertations
Background: Ethanol is a cardiotoxic substance that damages the heart by increasing apoptosis, free radical formation and calcium overloading. Consequently, there is an increase in cell death leaving fewer functioning myocytes leading to heart failure. Sildenafil is a phosphodiesterase type-5 (PDE-5) inhibitor approved for treatment of erectile dysfunction. Studies from our lab have demonstrated that PDE-5 inhibition reduces myocardial infarct size and attenuates post-ischemic cardiac dysfunction in both ischemia-reperfusion and permanent coronary artery ligation models. Therefore, in the present study, we hypothesized that treatment with sildenafil will prevent cardiotoxicity associated with acute alcohol exposure by reducing myocyte apoptosis and preserving …
Regulation Of Gastrointestinal Smooth Muscle Function By Hydrogen Sulfide, Ancy Nalli
Regulation Of Gastrointestinal Smooth Muscle Function By Hydrogen Sulfide, Ancy Nalli
Theses and Dissertations
Inhibitory neurotransmitters, chiefly nitric oxide and vasoactive intestinal peptide, cause MLC20 dephosphorylation and muscle relaxation via inhibition of myosin light chain (MLC) kinase and activation of MLC phosphatase. Hydrogen sulfide (H2S) produced as a byproduct by luminal sulfate-reducing commensal bacteria or as an endogenous signaling molecule synthesized from L-cysteine via cystathionine-γ-lyase (CSE) and cystathionine-β-synthase (CBS) regulates muscle contraction. However, the role of H2S in the regulation of MLC phosphatase activity and MLC20 phosphorylation is not known. The aim of the present study was to examine the expression of CSE and CBS in smooth muscle cells and to elucidate the molecular …
Studies On The Reaction Of High-Dose Hydroxocobalamin And Ascorbic Acid With Carbon Monoxide: Implications For Treatment Of Carbon Monoxide Poisoning, Joseph Roderique
Studies On The Reaction Of High-Dose Hydroxocobalamin And Ascorbic Acid With Carbon Monoxide: Implications For Treatment Of Carbon Monoxide Poisoning, Joseph Roderique
Theses and Dissertations
Based upon experimental evidence from the 1970’s we proposed that a reduced form of hydroxocobalamin should be capable of producing carbon dioxide (CO2) from carbon monoxide (CO) in blood, and that this conversion should be detectable. Using resonance raman spectroscopy we demonstrated that a mixture of hydroxocobalamin and ascorbic acid could create the reduced form of hydroxocobalamin. We used a closed-loop circulation system with a hollow-fiber membrane oxygenator to produce carboxyhemoglobin. Using sensitive gas monitoring equipment to the gas-out port of the oxygenator we analyzed the CO and CO2 concentrations coming from the oxygenator. The mixture of hydroxocobalamin and ascorbic …