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Theses and Dissertations

University of Wisconsin Milwaukee

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Dexterous Manipulation Capabilities Are Associated With Change In Discharge Rate Properties Of Motor Neurons With Age, Mukta N. Joshi May 2024

Dexterous Manipulation Capabilities Are Associated With Change In Discharge Rate Properties Of Motor Neurons With Age, Mukta N. Joshi

Theses and Dissertations

Aging is accompanied by declines in manual dexterity and fine motor control. The purpose of this research was to compare hand motor control in young and older adults and examine the neuromuscular mechanisms responsible for enabling these interactions. We test force variability during isometric and dynamic contractions, manual dexterity and track motor unit activity to identify the neuromuscular mechanisms responsible for changes in dexterity with age. 26 older adults (66-86 years) and 28 young adults (19 – 38 years) participated in the study. Research participants performed force matching tasks during index finger abduction, precision pinch, static pressing and hybrid force/ …

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Elucidating Aposematic Patterns In North American Hylids, Joseph S. Cannizzaro Iv Dec 2023

Elucidating Aposematic Patterns In North American Hylids, Joseph S. Cannizzaro Iv

Theses and Dissertations

Recognizing form and function of animal defenses is paramount to understanding the ecological and evolutionary forces behind predator and prey dynamics. Color patterns are strongly related to defensive strategies in animals. Some rely on camouflage to avoid detection, while others are brightly colored and conspicuously signal their noxiousness to potential predators. Still others combine cryptic dorsal coloration with colorful patches that are concealed in resting position but are facultatively unveiled by special behavior or simply during activity. Such hidden conspicuous color patches may be an intermediate stage in the evolution from camouflage to aposematism. We investigated whether conspicuously colored thighs …

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Biomolecular Function From Structural Snapshots, Roshanak Etemadpour Dec 2023

Biomolecular Function From Structural Snapshots, Roshanak Etemadpour

Theses and Dissertations

Biological molecules can assume a continuous range of conformations during function. Near equilibrium, the Boltzmann relation connects a particular conformation's free energy to the conformation's occupation probability, thus giving rise to one or more energy landscapes. Biomolecular function proceeds along minimum-energy pathways on such landscapes. Consequently, a comprehensive understanding of biomolecular function often involves the determination of the free-energy landscapes and the identification of functionally relevant minimum-energy conformational paths on these landscapes. Specific techniques are necessary to determine continuous conformational spectra and identify functionally relevant conformational trajectories from a collection of raw single-particle snapshots from, e.g. cryogenic electron microscopy (cryo-EM) …

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Methodology To Investigate Protein-Protein Interactions Of Jun During Neuronal Differentiation Of Pc12 Cells, Heather L. Leskinen Dec 2023

Methodology To Investigate Protein-Protein Interactions Of Jun During Neuronal Differentiation Of Pc12 Cells, Heather L. Leskinen

Theses and Dissertations

In humans and other mammals, injury to the central nervous system (CNS) can cause a permanent loss of neuronal function, leading to cognitive defects, limb paralysis, and other neurological disabilities. In contrast, studies have shown that some non-mammalian vertebrates like zebrafish, have the remarkable ability to functionally regenerate axons after CNS injury by reactivating and sustaining the expression of regeneration-associated genes (RAGs). Some RAGs encode transcriptional regulators that dimerize to control downstream gene expression necessary for functional axonal recovery. Our lab has previously identified Jun as an important transcriptional regulator of regeneration after optic nerve injury in zebrafish. After axon …

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Potential Moderating Impact Of Circulating Endocannabinoids On Resting State Networks And Post-Traumatic Stress Disorder, Emily Anne Albertina Dec 2023

Potential Moderating Impact Of Circulating Endocannabinoids On Resting State Networks And Post-Traumatic Stress Disorder, Emily Anne Albertina

Theses and Dissertations

Introduction: Traumatic injury increases risk for long-term adverse outcomes such as the development of post-traumatic stress disorder (PTSD). Research has found that risk for the development of PTSD after such an injury varies depending on individuals’ race/ethnic group and sex. Although prior work has explored relationships between injury and PTSD in these groups utilizing imaging paradigms (e.g., resting state functional connectivity) and biological paradigms (e.g., the endocannabinoid system), the relationship between these factors has been understudied. The present study explored the potential moderating effect circulating endocannabinoids may have on the relationship between resting state functional connectivity and post traumatic stress …

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Nutrient Dynamics Of Freshwater Estuarine Sediments Disturbed By Dredging, Ryan Allan John Roekle Aug 2023

Nutrient Dynamics Of Freshwater Estuarine Sediments Disturbed By Dredging, Ryan Allan John Roekle

Theses and Dissertations

This study examined the nutrient environment of sediments in the Milwaukee River estuary and the dynamics of those nutrients during simulated disturbance experiments within the context of large-scale dredging remediation. Surface sediments were collected from throughout the Milwaukee estuary (including river, harbor, and nearshore stations) by PONAR, centrifuged to separate porewater (interstitial water) from solid material, and filtered to further isolate and stabilize dissolved material. Porewaters were analyzed for dissolved nutrients including ammoniacal nitrogen (AN), nitrate, nitrite, and soluble reactive phosphorus (SRP). Surface sediment porewaters within the estuary were often highly enriched in AN and SRP, which were often 10-2000x …

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Investigation Of The Role Of The G-Protein-Coupled Estrogen Receptor In Memory Consolidation In Gonadectomized Male Mice, Gustavo Dalto Barroso Machado Aug 2023

Investigation Of The Role Of The G-Protein-Coupled Estrogen Receptor In Memory Consolidation In Gonadectomized Male Mice, Gustavo Dalto Barroso Machado

Theses and Dissertations

Estrogens are cholesterol-derived hormones that play crucial physiological and pathological roles in both sexes and across the lifespan. Many research groups have replicated the beneficial roles of 17-beta-estradiol (E2), the most potent estrogen, in memory consolidation in the past decades, even though some mechanisms are still unclear. The rapid effects of E2 in memory formation are attributed to its binding to different estrogen receptors (ER), notably the intracellular receptors ER and ER, as well as the membrane ER called G protein-coupled estrogen receptor (GPER). Previous work from our laboratory demonstrated that acute post-training infusion of E2 into the dorsal hippocampus …

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The Impact Of A Body Self-Compassion Program On Body Image And Experiences Of Exercise, Gretchen Paulson Aug 2023

The Impact Of A Body Self-Compassion Program On Body Image And Experiences Of Exercise, Gretchen Paulson

Theses and Dissertations

Body image concerns are a prevalent and pervasive issue among young adult women and are associated with a host of physical and psychological consequences including disordered eating, depression, and anxiety. Body image concerns play a role in physical activity and exercise behavior, where negative body image is related to lower levels of exercise engagement. Self-compassion is a way of relating to oneself with kindness and understanding, particularly in the presence of suffering or feelings of inadequacy. Self-compassion consists of three interrelated components: self-kindness, common humanity, and mindfulness, and has demonstrated effectiveness in reducing body image concerns, while fostering positive body …

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The Biological Bases Of Political Attachment: Neurobiological Correlates Of Ideology And Partisanship, Carisa Bergner Aug 2023

The Biological Bases Of Political Attachment: Neurobiological Correlates Of Ideology And Partisanship, Carisa Bergner

Theses and Dissertations

To fully understand the foundations of political attachments in an increasingly polarized environment, political scientists must reconcile traditional theories of political attitudes and behavior with insight gained from neurobiological approaches. The purpose of this research is to investigate the neurobiological correlates of strength of political ideology and partisanship, as well as the neurobiological correlates of ideological and partisan orientation. To do so, both structural and functional neuroimaging analyses were conducted on a diverse sample of patients at a Level 1 Trauma Center. Results indicate that strong ideological attachment is significantly associated with decreased volume in the left insula, though partisan …

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Using Music To Modify Step-Rate And Running Biomechanics In Healthy Runners, Erin Marie Lally Aug 2023

Using Music To Modify Step-Rate And Running Biomechanics In Healthy Runners, Erin Marie Lally

Theses and Dissertations

Context: Running-related injury (RRI) is a significant public health issue that may be caused by injurious running biomechanics. Increasing step-rate (SR) using gait retraining may prevent and treat RRI. The Optimizing Performance Through Intrinsic Motivation and Attention for Learning (OPTIMAL) theory indicates enhanced expectancies, autonomy, and external focus of attention will optimize motor learning. Music has been shown to create enhanced expectancies, can provide incidental choices (autonomy), directs attention externally, and may increase compliance. No studies have investigated if music can be used to alter SR and running biomechanics or strategies that may improve compliance to gait retraining. Objective: The …

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The Effects Of Task Complexity On Knee Mechanics And Joint Coordination Variability During A Side-Step Cutting Task, Carter Michael Schmitz Aug 2023

The Effects Of Task Complexity On Knee Mechanics And Joint Coordination Variability During A Side-Step Cutting Task, Carter Michael Schmitz

Theses and Dissertations

Injury to the anterior cruciate ligament (ACL) is a very common and debilitating injury suffered by athletes of all ages, genders, and abilities. Practitioners attempt to minimize risks by implementing ACL prevention programs designed to physically prepare athletes for the demands of sport, however, the success of these programs is very inconsistent. The majority of past ACL prevention programs prioritize constrained run and cut activities, the removal of motor variability - attempting to “idealize” mechanics - and limited task complexity. Due to the inherent complexities that exist within sport, it is possible that more task complexity and motor variability is …

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Impact Of The Pre-A Motif On Truncated Hemoglobin N Activity, Alexander Shayne Drena May 2023

Impact Of The Pre-A Motif On Truncated Hemoglobin N Activity, Alexander Shayne Drena

Theses and Dissertations

Tuberculosis (TB) remains the leading cause of death by an infectious agent and therefore a global health crisis, according to the most recent report by the World Health Organization. This is due, in part, to Mycobacterium tuberculosis’ impressive defensive mechanisms against immune response, as well as the rise of Multi-Drug Resistant strains that have recently developed. Towards the turn of the century, a small heme protein called truncated hemoglobin N (trHbN) was discovered to protect the bacteria against reactive nitrogen species by converting nitric oxide (NO) to nitrate at rates far exceeding those of myoglobin and closer to those of …

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Exploring The Perceived Psychosocial Experiences Of Recreational Runners With Patellofemoral Pain: A Grounded Theory Approach, Kenneth Ildefonso May 2023

Exploring The Perceived Psychosocial Experiences Of Recreational Runners With Patellofemoral Pain: A Grounded Theory Approach, Kenneth Ildefonso

Theses and Dissertations

Patellofemoral pain (PFP) is a specific type of patellar or retropatellar pain aggravated by at least one physical activity that loads the patellofemoral joint during weight bearing on a flexed knee such as running (Crossley et al., 2016). Previous PFP research has highlighted the importance of psychosocial variables among PFP populations including recreational runners (Vicenzino et al., 2022). Upon reviewing the existing literature, much of it appears to be atheoretical and lack construct clarity. The purpose of this dissertation was to explore the perceived psychosocial experiences of recreational runners with PFP. This dissertation aimed to (1) document recreational runners’ perceived …

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Estimating Energy Cost Of Physical Activities From Video Using 3d-Cnn Networks, Pragya Shrestha Chansi May 2023

Estimating Energy Cost Of Physical Activities From Video Using 3d-Cnn Networks, Pragya Shrestha Chansi

Theses and Dissertations

This research proposes a machine learning model that can estimate the energy cost of physical activities from video input. Currently, wearable sensors are commonly used for this purpose, but they have limitations in terms of practicality and accuracy. A deep learning model using three dimensional convolutional neural network (3D-CNN) architecture was used to process the video data and predict the energy cost in terms of metabolic equivalents (METs). The proposed model was evaluated on a dataset of physical activity videos and achieved an average accuracy of 71% on energy category prediction task and an root mean squared error (RMSE) of …

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Body Bound, Rachel Allison May 2023

Body Bound, Rachel Allison

Theses and Dissertations

Body Bound is an exhibition catalog that corresponds to an exhibition of objects on display in the Emile Mathis Gallery that opened on February 23, 2023. The Exhibition traces the historically grounded and long-standing tradition of using bodily material as the basis for bookmaking. This practice has not subsided entirely in its traditional form but has also branched off and informed contemporary book-making practices. Contemporary books, specifically artist books, are a part of a longer history of using and presenting bodies with books. This exhibition includes historical books and contemporary artist books from the UWM special collections as well as …

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Monitoring Welfare In Captive Chimpanzees (Pan Troglodytes) Using Individual Positional Behavior And Substrate Use Profiles, Joseph Lara May 2023

Monitoring Welfare In Captive Chimpanzees (Pan Troglodytes) Using Individual Positional Behavior And Substrate Use Profiles, Joseph Lara

Theses and Dissertations

The welfare of captive chimpanzees partly depends on the structural features present in their enclosure. An individual’s manner of expressing positional behaviors depends on these environmental characteristics and may be reflective of their physical and mental health. This thesis seeks to further the scientific understanding of the relationships between positional behavior, substrate use and captive chimpanzee welfare. In pursuit of this goal, I designed and installed a novel vertical climbing aid onto a climbable platform structure within an enclosure at the chimpanzee sanctuary, Chimp Haven, in an effort to encourage mobility and vertical space use in the enclosure’s residents. Additionally, …

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Educational Environments And Children's Physical Activity And Sedentary Behaviors, Nathan R. Tokarek May 2023

Educational Environments And Children's Physical Activity And Sedentary Behaviors, Nathan R. Tokarek

Theses and Dissertations

Children and adolescents spend the majority of their day engaged in sedentary behaviors (SB), while also not meeting physical activity (PA) recommendations. The failure to develop and maintain health enhancing behaviors from a young age may impact an individual throughout their life. With children and adolescents spending a large proportion of their waking hours in a school setting, the educational environment presents an opportunity in which children and adolescents’ PA and SB can be positively influenced. Therefore, the purpose of this dissertation was to explore how children and adolescent’s school time PA and SB are associated with the educational setting, …

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Stress And Parity Among Female Vervet Monkeys (Chlorocebus Pygerythrus) At Two Sites In South Africa, Jo Gansemer Dec 2022

Stress And Parity Among Female Vervet Monkeys (Chlorocebus Pygerythrus) At Two Sites In South Africa, Jo Gansemer

Theses and Dissertations

This thesis examines the hypothesis that in both Soetdoring and !Gariep populations, a femaleSouth African vervet's parity status (nulliparous or parous) affects her physiological stress levels. Vervet monkey ecology and life history are examined to contextualize the relationship between stress and parity. The testing of this hypothesis involves the analysis of hair cortisol concentrations (HCC) of samples from nulliparous and multiparous adult females of the two different South African sites. The HCC was statistically analyzed to look for correlations between stress, parity status, and site. No significant relationship between stress and parity status was found. However, significant relationships were found …

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Microbial Ecology Of Urban Sewers, Emily Lou Lamartina Dec 2022

Microbial Ecology Of Urban Sewers, Emily Lou Lamartina

Theses and Dissertations

Municipal sewage provides a glimpse into the health and activities of a human society. For more than a century, sewage exploration has helped expose the sources of disease outbreaks and track disease progression over time. Recent advancements in wastewater surveillance born from the COVID-19 pandemic have potential to enhance mitigation efforts against the decades-long global health crisis of microbial antibiotic resistance. However, critical knowledge gaps exist in wastewater surveillance, stemming from a lack of understanding in sewer microbial ecology. Ecology reveals trends in how communities respond and adapt to change, which has far-reaching implications for identifying effective strategies for disease …

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The Evergreen Challenges Of Healing: An Evaluation Of Urban Green Space In Harambee, Kacee Ochalek Dec 2022

The Evergreen Challenges Of Healing: An Evaluation Of Urban Green Space In Harambee, Kacee Ochalek

Theses and Dissertations

Urban green space initiatives have emerged in low- and middle-income cities as a solution to disinvestment, the production of more positive public health metrics, and a tool of community engagement. While the production of urban greening provides ample room for applause, The City of Milwaukee’s Healing Spaces Initiative model regarding the ongoing construction of green space and the implementation of maintenance creates challenges that perpetuate racist capitalistic notions of the neoliberal project. This paper presents reports from participant observation, document analysis, and 17 qualitative interviews with representatives of the City of Milwaukee, community partners, garden leaders, and residents. The data …

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Deciphering The Firing Patterns Of Hippocampal Neurons During Sharp-Wave Ripples, Kourosh Maboudi Ashmankamachali Dec 2022

Deciphering The Firing Patterns Of Hippocampal Neurons During Sharp-Wave Ripples, Kourosh Maboudi Ashmankamachali

Theses and Dissertations

The hippocampus is essential for learning and memory. Neurons in the rat hippocampus selectively fire when the animal is at specific locations - place fields - within an environment. Place fields corresponding to such place cells tile the entire environment, forming a stable spatial map supporting navigation and planning. Remarkably, the same place cells reactivate together outside of their place fields and in coincidence with sharp-wave ripples (SWRs) - dominant electrical field oscillations (150-250 Hz) in the hippocampus. These offline SWR events frequently occur during quiet wake periods in the middle of exploration and the follow-up slow-wave sleep and are …

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Characterization Of Antimicrobial Activity Of The Symbiotic Bacterium Xenorhabdus Szentirmaii, Miliya Ansad Aug 2022

Characterization Of Antimicrobial Activity Of The Symbiotic Bacterium Xenorhabdus Szentirmaii, Miliya Ansad

Theses and Dissertations

Xenorhabdus species belonging to the Enterobacteriaceae family are gram negative bacteria that engage in symbiotic associations with the entomopathogenic soil dwelling nematodes of the family Steinernematidae. In this bacterium–nematode mutualistic interaction, both partners benefit from the association. By entering and perforating the insect gut and translocating to the body cavity of hemocoel, the nematodes gain access to the nutrient resource of the insect hemolymph. Xenorhabdus spp are released in the hemolymph in which they produce toxins and immunosuppressive compounds that kill the insect. During the infection Xenorhabdus spp grow to high cell density in the hemolymph. As the nematode invades …

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The Effects Of Cheerleading Surfaces On Landing Characteristics During Vertical And Flip Landings, Anthony Vu Nguyen Aug 2022

The Effects Of Cheerleading Surfaces On Landing Characteristics During Vertical And Flip Landings, Anthony Vu Nguyen

Theses and Dissertations

Lateral ankle sprains are common injuries in cheerleading and typically occurring during tumbling. The landing surface can impact injury risk by modulating loading parameters and risky joint positions. Most surface-landing studies have used vertical landing tasks to study acrobatic populations, and few studies have compared vertical and flipping tasks. Therefore, the purpose of this study was to compare the effects of two cheerleading surfaces between vertical drop landing and flip landing tasks. Doing so explained surface characteristic influences on landing and the validity of using vertical landing tasks to represent acrobatic sports. Twelve collegiate cheerleaders (7 females, 5 males; age: …

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Complex Regulatory Pathways Connect Vfm Quorum-Sensing And Cyclic-Di-Gmp Signaling To Bacterial Virulence In The Phytopathogen Dickeya Dadantii 3937, Biswarup Banerjee Aug 2022

Complex Regulatory Pathways Connect Vfm Quorum-Sensing And Cyclic-Di-Gmp Signaling To Bacterial Virulence In The Phytopathogen Dickeya Dadantii 3937, Biswarup Banerjee

Theses and Dissertations

The bacterial second messenger Bis-(3’-5’)-cyclic dimeric guanosine monophosphate (c-di-GMP) regulates multiple cellular behaviors in most bacteria. Bacterial c-di-GMP signaling involves enzymes that synthesize and degrade c-di-GMP, c-di-GMP binding effectors, and targets that are acted upon by the effectors. So far, the c-di-GMP signaling pathways have been understudied. In this work, we explore the c-di-GMP signaling network further in the phytopathogen Dickeya dadantii 3937. In Chapter 2, we identified VfmE as a c-di-GMP binding transcriptional activator that represses pectate lyase production under a high c-di-GMP condition. VfmE was found to bind c-di-GMP in vitro via the RxxxR motif, similar to PilZ …

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Probing Hippocampal Activity In Sleep, Sleep Deprivation, And Recovery Sleep, Bapun Giri Aug 2022

Probing Hippocampal Activity In Sleep, Sleep Deprivation, And Recovery Sleep, Bapun Giri

Theses and Dissertations

Long-term memories are established over several hours of sleep following their initial acquisition. Large-scale recordings from the hippocampus have revealed that neurons reactivate or `replay' wake-induced activity in subsequent sleep episodes, leading many to suggest replay as an underlying mechanism for sleep-mediated memory consolidation. In this dissertation, I describe experiments that examine the extent of hippocampal replay during sleep and how replay is affected in the absence of sleep. In Chapter 2, we found that the hippocampus reactivates neuronal patterns expressed during exploration of a novel environment for upto 10 hours in subsequent sleep with a half-maximum timescale of $~$6 …

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Towards Environmental Sustainability Of Nanotechnology Through Improved Evaluations Of Impact And Risk To Aquatic Species: Cross-Species Comparisons From The Laboratory And Environmentally Realistic Exposure Scenarios In The Field, Becky J. Curtis May 2022

Towards Environmental Sustainability Of Nanotechnology Through Improved Evaluations Of Impact And Risk To Aquatic Species: Cross-Species Comparisons From The Laboratory And Environmentally Realistic Exposure Scenarios In The Field, Becky J. Curtis

Theses and Dissertations

Engineered nanomaterials (ENMs) are utilized in a wide variety of applications and products, including everything from toothpaste and personal care products to industries such as aerospace, defense, medicine, electronics, and agriculture. Depending on the application, release of ENMs to the environment may occur through both unintentional and deliberate routes. High surface-area-to-volume ratios make ENMs more volatile than bulk counterparts, and their small size allows translocation within biological systems that would not be possible with larger materials. These and other characteristics make ENM-biological system interactions unpredictable, creating uncertainty about their potential risks to environmental health. The purpose of this research is …

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Influence Of Nutrients And The Native On E. Coli Survival In The Beach Environment, Brigid Meyers May 2022

Influence Of Nutrients And The Native On E. Coli Survival In The Beach Environment, Brigid Meyers

Theses and Dissertations

E. coli is used as an indicator for water quality to determine if water poses a health risk for pathogens. Past research has shown that E. coli is present in high numbers in freshwater beach sands distinct from fecal pollution events, yet the precise mechanism for their persistence in not well understood. Persistent E. coli populations in sand can resuspend into adjacent water and lead to increased beach closures when no threat is present. This work identifies factors that influence the survival of E. coli in sand using laboratory microcosms to replicate beach conditions. Microcosms were deployed to examine the …

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Molecular And Behavioral Impacts Of Treated Wastewater Effluent On Fish, Peter Schumann May 2022

Molecular And Behavioral Impacts Of Treated Wastewater Effluent On Fish, Peter Schumann

Theses and Dissertations

Currently, most studies that focus on the sublethal impacts of contaminants of emerging concern (CECs) such as pharmaceuticals utilize a single-compound approach, whereas these compounds always occur as complex mixtures in the environment. Wastewater treatment plants (WWTPs) are a major contributor to the formation of these mixtures as current treatment technologies inadequately remove many chemical pollutants. There is a paucity of knowledge on the neurological impacts due to exposure to complex CEC mixtures resulting from the discharge of treated WWTP effluent into aquatic environments. This knowledge gap leaves incomplete assessments of the hazards and risks from exposure to treated effluent …

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Spline Modeling And Localized Mutual Information Monitoring Of Pairwise Associations In Animal Movement, Andrew Benjamin Whetten May 2022

Spline Modeling And Localized Mutual Information Monitoring Of Pairwise Associations In Animal Movement, Andrew Benjamin Whetten

Theses and Dissertations

to a new era of remote sensing and geospatial analysis. In environmental science and conservation ecology, biotelemetric data recorded is often high-dimensional, spatially and/or temporally, and functional in nature, meaning that there is an underlying continuity to the biological process of interest. GPS-tracking of animal movement is commonly characterized by irregular time-recording of animal position, and the movement relationships between animals are prone to sudden change. In this dissertation, I propose a spline modeling approach for exploring interactions and time-dependent correlation between the movement of apex predators exhibiting territorial and territory-sharing behavior. A measure of localized mutual information (LMI) is …

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The Effects Of An R103q Mutation On The Chemical And Physical Properties Of The Enzyme Cytochrome C Nitrite Reductase (Ccnir), Shahama Alam May 2022

The Effects Of An R103q Mutation On The Chemical And Physical Properties Of The Enzyme Cytochrome C Nitrite Reductase (Ccnir), Shahama Alam

Theses and Dissertations

Cytochrome c Nitrite Reductase (ccNiR) is a periplasmic homodimeric decaheme enzyme that catalyzes the reduction of nitrite to ammonium in a process that involves six electrons and eight protons. The project described herein explored the properties of the Shewanella oneidensis R103Q ccNiR variant and compared them to the properties of the wild type enzyme. Under standard assay conditions, which use the strong reducing agent methyl viologen monocation radical (MVred) as an electron source, the R103Q variant still catalyzes reduction of nitrite to ammonium, albeit with an MVred turnover rate that is 20% of the wild type’s (780 ± 50 s-1 …

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