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Articles 721 - 750 of 246803
Full-Text Articles in Life Sciences
Evaluation Of Interior Least Tern (Sterna Antillarum Anthalassos) Colony Success Rate And Distance Upstream On The Arkansas River, Coley Turner, Nathan Mansor, Shelby Hunter
Evaluation Of Interior Least Tern (Sterna Antillarum Anthalassos) Colony Success Rate And Distance Upstream On The Arkansas River, Coley Turner, Nathan Mansor, Shelby Hunter
ATU Research Symposium
The previously endangered Interior Least Terns (or LETEs) are currently being monitored to ensure their populations are increasing. The purpose of this study was to provide current data to inform management decisions that must be made during their migration season. Sandbars where LETEs colonized were assigned a number corresponding to river mile. Fledglings per Breeding pair Rate (FBR) was calculated by dividing peak fledgling count by half of the adult count. A total of 798 adult LETEs and 228 fledglings were observed on sandbars. A colony was considered ‘failed’ if it had an FBR of 0, and ‘successful’ for anything …
Arkansas Soil Erosion And Conservation Methods In Ornamental Landscapes, Abby J. Cutsinger
Arkansas Soil Erosion And Conservation Methods In Ornamental Landscapes, Abby J. Cutsinger
ATU Research Symposium
This study will attempt to identify the best soil erosion mitigation methods for Arkansas ornamental landscape settings by comparing the strengths and weaknesses of known techniques. Since soil is not a renewable resource, we must apply conservation practices wherever possible, not just in agricultural settings. To determine the most effective erosion prevention techniques, this study will review soil studies conducted by the NRCS and NASA, as well as articles on best practices observed in ornamental landscape and agricultural industries. These are analyzed by comparing and contrasting techniques against known problems with Arkansas soil to determine which methods are most effective. …
Microhabitat Use Of The Highland Darter (Etheostoma Teddyroosevelt) In The Illinois Bayou Watershed Of Arkansas, Colton W. Morris
Microhabitat Use Of The Highland Darter (Etheostoma Teddyroosevelt) In The Illinois Bayou Watershed Of Arkansas, Colton W. Morris
ATU Research Symposium
Highland Darters (Etheostoma teddyroosevelt) are an Ozark regional endemic species, occurring in the Arkansas River and upper White River systems of Arkansas, Kansas, Oklahoma, and Missouri. There have been very few studies on this species, specifically those that have quantified the habitat use of this species across its range. A new study was needed to fill in the knowledge gap of microhabitat use by the Highland Darter in Arkansas. We wanted to address this knowledge gap by assessing the microhabitat use of this species in the Illinois Bayou watershed in Arkansas. Highland Darters were observed during snorkel surveys at known …
Mussel Communities Of Two Impacted Tributaries In The Arkansas South Central Plains Ecoregion, Jimmy Hall, Seth Drake, Parker Brannon, Savannah Wise
Mussel Communities Of Two Impacted Tributaries In The Arkansas South Central Plains Ecoregion, Jimmy Hall, Seth Drake, Parker Brannon, Savannah Wise
ATU Research Symposium
Anthropogenic influences such as mining and water quality degradation from wastewater effluents and agricultural runoff heavily impact freshwater mussel communities. The South-Central Plains (SCP) Ecoregion in Arkansas contains the Smackover Formation, an area that has been exploited for its oil with high frequency of drilling. In 1922 the Smackover pool in Union County was found and quickly became one of the world's most productive oil sites. Hurricane Creek and Smackover Creek, two tributaries within the Ouachita River basin, have experienced negative impacts from oil exploration and mining. However, to our knowledge, mussel communities in these creeks have not been assessed …
A Comparative Study On The Effects Of Meishan And Duroc Teaser Boars On Farrowing Rates Of Two Individual Swine Operations, Allie Shewmake, Payton Ham
A Comparative Study On The Effects Of Meishan And Duroc Teaser Boars On Farrowing Rates Of Two Individual Swine Operations, Allie Shewmake, Payton Ham
ATU Research Symposium
Effective use of certain teaser boar breeds in artificial insemination, a common assisted reproductive procedure, in swine production provides a surmountable outlet to increase farrowing rates, breeding outcomes, and economic gain within the swine industry. Previous studies have emphasized the importance of boar types in swine breeding outcomes; however, this study delves into the divergent characteristics of Meishan and Duroc boars and how their different attributes affect the farrowing rates of Yorkshire-landrace sows at two individual swine operations, a smaller independent facility, and a larger commercial facility. Data was garnered and contrasted for three-year (2015-2017) time spans from each operation. …
Evaluating The Importance Of Biosecurity In Swine Operations, Lindsey Lemley, Lilly Rogers, Austin Calhoun
Evaluating The Importance Of Biosecurity In Swine Operations, Lindsey Lemley, Lilly Rogers, Austin Calhoun
ATU Research Symposium
Swine health is critical to maintaining a profitable and productive swine operation. Biosecurity and sanitation are important practices that help maintain herd health. This study aimed to discover how biosecurity protocols and management practices differ between swine operations of different sizes. With diseases such as Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), Brucellosis, and Porcine Circovirus type 2 (PCV2) being common in the swine industry, a strong biosecurity protocol protects swine farmers from financial losses associated with disease outbreaks. In 2004 alone, the estimated financial loss to PRRSV was over $761 million. Data concerning biosecurity and sanitation was collected from …
Length-Weight Relationship Of Flier (Centrarchus Macropterus) In Moro Creek, Jeffrey G. Phillips
Length-Weight Relationship Of Flier (Centrarchus Macropterus) In Moro Creek, Jeffrey G. Phillips
ATU Research Symposium
Length-weight relationships provide foundational knowledge for managing fisheries populations. However, there are many species where the length-weight relationships are not widely understood. The Flier (Centrarchus macropterus) is one species where there is a lack of information regarding its length-weight relationship and spatial variation associated with this relationship. A new study was needed to assess the length-weight relationship for this species. Fliers were collected from upper Moro Creek during October 2023. Multiple gears (backpack electrofishing and seining) were employed to increase detection of this species. Length (mm), and weight (g) were recorded for every individual observed. A total of 85 Flier …
Alleviating Environmental Stresses In Native Arkansas Plants By Arbuscular Mycorrhizal Fungi Inoculation, Rebecca C. Morse
Alleviating Environmental Stresses In Native Arkansas Plants By Arbuscular Mycorrhizal Fungi Inoculation, Rebecca C. Morse
ATU Research Symposium
The rapid human-driven changes in the environment during the Anthropocene have placed extreme stress on many native plants and animals. Beneficial interactions with microorganisms may be crucial for ameliorating these stressors and facilitating the ecosystem services host organisms provide. The role of an important mutualistic association with arbuscular mycorrhizal fungi (AMF) in the competitive ability and successful adaptation of Arkansas native species by enhancing nutrient uptake has not been previously considered. Analysis of field root and soil samples from the native species in their native range as well as a controlled green-house experiment will be conducted to determine the level …
A Survey Of Mussels In Small Tributaries Of The Ouachita River Headwaters, Aaron J. Huckeba, Seth Drake, Kendall Moles, Risa Mccollough, Nathan Mansor
A Survey Of Mussels In Small Tributaries Of The Ouachita River Headwaters, Aaron J. Huckeba, Seth Drake, Kendall Moles, Risa Mccollough, Nathan Mansor
ATU Research Symposium
Understanding species distributions is of utmost importance for effective conservation of aquatic resources. Freshwater mussels are among the most imperiled taxonomic groups as they are experiencing rapid declines in the southeastern United States. Standard mussel surveys are typically conducted on larger streams and rivers, leaving small headwater tributaries unsampled. Our study looked to document mussels in headwater tributaries of the Ouachita River watershed. We conducted standardized time-based surveys at 19 sites across nine tributaries using snorkeling, grubbing, and raking methodologies in the summer of 2023. Furthermore, we collected measurements on water quality (pH, conductivity, salinity, total dissolved solids, and temperature) …
Bat Survey Of Winona, Hailey B. Christensen, Morgan L. Winter
Bat Survey Of Winona, Hailey B. Christensen, Morgan L. Winter
Research & Creative Achievement Day
Two bat detectors were placed at Garvin Heights and West Lake Winona to test bat activity in Winona, Minnesota. It was hypothesized that there would be a higher activity of bats at West Lake Winona than there would be at Garvin Heights. It was also hypothesized that high moonlight illumination would impact bat activity in a negative way. After data analyses were performed, it was found that bats were more active at West Lake Winona than at Garvin Heights. Based on the data analyses for moon light illumination and bat activity, there is no pattern common to all species, but …
Post Rehabilitation Analysis Of Stream Habitat And Brown Trout In Cedar Valley Creek, Ashlynn D. Leonard, Emma C. Ehlers
Post Rehabilitation Analysis Of Stream Habitat And Brown Trout In Cedar Valley Creek, Ashlynn D. Leonard, Emma C. Ehlers
Research & Creative Achievement Day
A section of Cedar Valley Creek went through a rehabilitation process that was intended to improve the trout habitat as well as to reinforce banks, deepen the stream, and narrow the channel. We took brown trout (Salmo trutta) counts and did habitat analyses at five locations (each 50 m long), in the restored stream section of Cedar Valley Creek during fall 2023 to compare to pre-rehabilitation data collected in 2022. A backpack electrofisher and nets were used to collect brown trout via a single-pass removal sampling process. All trout were measured for total length (mm) before being released back into …
Changes In Animal Vocalizations In Response To A Total Solar Eclipse, Colton W. Morris
Changes In Animal Vocalizations In Response To A Total Solar Eclipse, Colton W. Morris
ATU Research Symposium
My proposed research project will study the changes in animal vocalizations in response to the total solar eclipse that will take place in Russellville, Arkansas, on April 8, 2024. I will use audio recordings before, during, and after the eclipse to quantify changes in animal vocalizations. Most recordings will be in the range of normal hearing, and will primarily detect birds, frogs, and insects. Additionally, I will deploy one ultrasonic recorder capable of detecting bats. From these recordings I will be able to quantify overall and species-specific call rates. Audio recordings will be paired with continuous measurements of light intensity …
Molecular Methods Used To Identify A New Species Of Dictyocaulus (Family Dictyocaulidae) In White-Tailed Deer, Garit T. Wollan, Elisa M. Quevedo
Molecular Methods Used To Identify A New Species Of Dictyocaulus (Family Dictyocaulidae) In White-Tailed Deer, Garit T. Wollan, Elisa M. Quevedo
Research & Creative Achievement Day
Lungworms (Dictyocaulus) are a type of parasitic nematode found in the lungs of vertebrates and can cause life threatening diseases to their host(s). Dictyocaulus is a type of lungworm found in cattle (Bos taurus), deer (Cervidae), goat (Capra aegagrus hircus), sheep (Ovis aries), and horses (Equus caballus). The purpose of this study was to use DNA analysis to distinguish between different species of Dictyocaulus among cattle, white-tailed deer (Odocoileus virginianus), and red deer (Cervus elaphus). Adult lungworms were collected from the lungs of cattle from research farms in Missouri, Wisconsin, and Mississippi, from the lungs of red deer from a …
Characterization Of Bacteria And Fungi In Hotel Bathroom Products, Madie Clarke, Regan L. Feit, Rebecca A. Nachreiner
Characterization Of Bacteria And Fungi In Hotel Bathroom Products, Madie Clarke, Regan L. Feit, Rebecca A. Nachreiner
Research & Creative Achievement Day
Refilling hotel products and storing open products in bathrooms are excellent conditions for bacterial growth. Bacteria accumulate in frequently touched surfaces and thrive in warm, moist, nutrient-rich environments. Bathrooms are also the perfect breeding ground for microbes to flourish. Not all bacteria are bad for our health, but it would be beneficial to know what microorganisms can be found in cosmetic or cream products that are used on a daily basis. This is especially important when bacteria or fungi are known pathogens. This study evaluated and classified bacteria and fungi in shampoos and body lotions using Trypticase Soy Agar (TSA) …
Detection Of Lyme's Disease Caused By Borrelia Burgdorferi In Ixodes Scapularis Using A Pcr Multiplex And Real-Time Pcr, Emily T. Dittman, Bethany G. Basile
Detection Of Lyme's Disease Caused By Borrelia Burgdorferi In Ixodes Scapularis Using A Pcr Multiplex And Real-Time Pcr, Emily T. Dittman, Bethany G. Basile
Research & Creative Achievement Day
Ixodes scapularis (deer ticks) are a vector for the bacterium Borrelia burgdorferi that is known to cause Lyme's Disease in animals and humans (Homo sapiens). In the past few years, there has been an increase in the prevalence of Lyme's Disease cases throughout the midwestern United States indicating that many ticks have been infected with B. burgdorferi. Using I. scapularis DNA samples collected from white-tailed deer (Odocoileus virginianus) in the spring of 2006 from Buffalo County, WI and Winona County, MN, both traditional and real-time PCR methods were used to test for the presence of the B. burgdorferi organism in …
Morphology Of Trematodes In Gadwall Ducks (Mareca Strepera) Found In The Mississippi Valley: Parasitology Class Project, Annika N. Lensch, Ethan B. Gjervik, Mckenna A. Baker, Elizabeth K. Haumont, Mivang Xiong
Morphology Of Trematodes In Gadwall Ducks (Mareca Strepera) Found In The Mississippi Valley: Parasitology Class Project, Annika N. Lensch, Ethan B. Gjervik, Mckenna A. Baker, Elizabeth K. Haumont, Mivang Xiong
Research & Creative Achievement Day
Gadwall ducks (Mareca strepera) are commonly found in lakes, ponds, and marshes west of the Mississippi River in summer months. A Gadwall duck is considered a dabbler duck which feeds on aquatic plants floating on the water's surface occasionally feeding on insects, crustaceans (crayfish, crabs), and small fish. Trematodes are a commonly found parasite in the gastrointestinal tract of many species of ducks. The ducks used in this study were legally collected and donated by hunters from Buffalo City, Wisconsin along the Mississippi River. This study will focus on identifying trematodes found within Gadwall ducks such as Echinostoma, Zygocotyle, and …
Carbon Sequestration And Its Role In Mitigating Climate Change, Mallory Woodward
Carbon Sequestration And Its Role In Mitigating Climate Change, Mallory Woodward
ATU Research Symposium
Carbon sequestration is when carbon dioxide is stored in ecosystems like grasslands forests, soils and oceans. Wetlands are one of the many important ecosystems that sequester carbon and act as a carbon sink. The U.S. Energy Information Administration estimates that in 2019 33,621.5 million metric tons of carbon were released into the atmosphere globally, due to human emissions. Anthropogenic carbon emissions have rapidly increased over the past 150 years. Since 1982, The global rate of warming is more than three times as fast, currently rising 0.36 degrees Fahrenheit each decade, and the rate will continue to increase. How do wetlands …
Microbiological Study Of Used Cosmetic Products, Macy S. Priest, Rachel L. Przybilla
Microbiological Study Of Used Cosmetic Products, Macy S. Priest, Rachel L. Przybilla
Research & Creative Achievement Day
The bacterial and fungal load in commercial creams and cosmetic products -was examined. This research aimed to identify pathogenic microbes, their toxin production, and their potential impact on product integrity. Our tests showed bacterial growth in the beauty products Cetaphil Facial Cleanser, CereVe moisturizing lotion, and Maybelline facial powder. Several microbes were characterized: a gram-positive bacteria identified as Bacillus cereus as well as an unknown gram-negative bacteria. In addition, the fungus Fusarium was observed. These microbes might be commonly observed in beauty products, but theoretically many different species of bacteria could grow in this type of environment.
Genetic Association In Entylia Carinata Using Random Forest Classification, Caden J. Harper
Genetic Association In Entylia Carinata Using Random Forest Classification, Caden J. Harper
Research & Creative Achievement Day
The goal of this research was to identify locations in the genome of the Entylia carinata, known as the treehopper, that are associated with anomalous behavior exhibited by the species. Treehoppers are phytophagous insects and are shown to feed, reproduce, and rear their young on specific aster species. Observation has shown that the insects will disregard potential mates in close proximity in favor of those that originate from the same plant species as themselves. This behavior suggests genetic separation in the species based on plant nativity and warrants genetic analysis. Machine learning offers an effective genetic association technique due to …
Denial Of Pregnancy Or Cryptic Pregnancy, Nadia N. Shuriah, Elaine Mcfarland, Bethany Spenn, Kaisha Butz Mcmanus
Denial Of Pregnancy Or Cryptic Pregnancy, Nadia N. Shuriah, Elaine Mcfarland, Bethany Spenn, Kaisha Butz Mcmanus
BIO4960: Practicum in Biology I
Denial of pregnancy, or cryptic pregnancy, presents a complex situation where individuals are unaware of their pregnancy until the late stages of labor. This study delves into the multifaceted nature of concealed and denied pregnancies, exploring their prevalence, factors contributing to them, and their implications for both individuals and healthcare systems. By reviewing relevant literature and case studies, we aim to illuminate the difficulties healthcare providers face in identifying and managing cases of denial of pregnancy. Additionally, we stress the significance of proactive screening and comprehensive care to ensure the well-being of mothers and infants. This research endeavors to deepen …
Exploring The Longitudinal Effects: Non-Traditional Pedagogies And Practical Learning On Biology Education And Medical Understanding, Thomas Ziebro
Exploring The Longitudinal Effects: Non-Traditional Pedagogies And Practical Learning On Biology Education And Medical Understanding, Thomas Ziebro
Honors Projects
Medical disinformation is becoming a rampant problem in the United States, and the Covid-19 pandemic highlighted it. There have been attempts to fight the problem at the source through fact verification. These attempts have been unsuccessful. This paper investigates the efficacy of non-traditional pedagogies and the correlation between them and an individual perceived and practical ability to understand their primary care provider. The purpose of this research was to establish whether non-traditional pedagogies at the secondary and post-secondary level are more effective than traditional lecture-based instruction. The research was conducted through an anonymous cross-sectional survey which included questions about educational …
Ethanol-Induced Alterations To Astrocytic Glutamate Transporter Mrna Expression In The Mpfc Of Adult Mice, Zachary Evans
Ethanol-Induced Alterations To Astrocytic Glutamate Transporter Mrna Expression In The Mpfc Of Adult Mice, Zachary Evans
Undergraduate Honors Theses
The medial prefrontal cortex (mPFC) is one of several brain structures that is contained in the mesolimbocortical pathway and controls many processes involved in alcohol use disorder (AUD). There is vast glutamatergic output from the mPFC to other mesolimbocortical regions; neuroplastic changes to this system are believed to be closely tied to the development and maintenance of AUD. Astrocytes are key regulators of glutamatergic transmission through their uptake of excess glutamate from the synapse and recycling back into neurons. Ethanol (EtOH) alters the expression of astrocytic genes that encode for transporters that control glutamate homeostasis; these include the glutamate transporter-1 …
Simple Sprinkler Performance Testing For Rich County, Kelly Kopp, L. Neil Allen, Dallen Smith, Lauren Thomson
Simple Sprinkler Performance Testing For Rich County, Kelly Kopp, L. Neil Allen, Dallen Smith, Lauren Thomson
All Current Publications
This fact sheet describes how to perform a site inspection and a sprinkler test so you can irrigate your landscape more efficiently. It also provides an irrigation schedule for Rich County, Utah.
Metabolic Scaling Of Aquatic Macroinvertebrates, Jacob Woelber
Metabolic Scaling Of Aquatic Macroinvertebrates, Jacob Woelber
Honors Thesis
The common assumption in regard to metabolic rate scaling with body size is the 3/4 law, which predicts a scaling exponent of 0.75 between log metabolic rate and log body size. Supporting evidence exists for this theory on a large, general scale. However, factors such as temperature, predation, and environment can cause the scaling exponent to deviate from 0.75. This paper takes a closer look at the effects of temperature and predation on metabolic rates via oxygen consumption in freshwater macroinvertebrate populations. To do this, 24 tanks were filled with water to allow natural populations of macroinvertebrates (predominantly Chironomidae) to …
Water Lily Leaf Beetle Ecology On Hourglass Lake In Big Lake, Alaska, Haley Lloyd, Grace Beatty
Water Lily Leaf Beetle Ecology On Hourglass Lake In Big Lake, Alaska, Haley Lloyd, Grace Beatty
Scholar Week 2016 - present
The Water-Lily Leaf beetle, Galerucella nymphaeae, is a beetle commonly found on Western Pond Lily pads, Nuphar lutea, in Hourglass Lake. Hourglass Lake is located in Big Lake, Alaska. This area is surrounded by boreal forest and bogs and is situated roughly twenty miles North of Anchorage. Within this ecosystem, the Water-Lily Leaf Beetle and the Western Pond Lily are intrinsically linked, as the lily pads serve as a site of feeding and reproduction for the beetles. Dr. Derek Rosenberger of Olivet Nazarene University noticed an abundance of these beetles as he was kayaking through Hourglass Lake, and a project …
Comparing Pollinator Communities: Onu’S Swanberg Sanctuary V. Midewin National Tallgrass Prairie, Stephanie Clark
Comparing Pollinator Communities: Onu’S Swanberg Sanctuary V. Midewin National Tallgrass Prairie, Stephanie Clark
Scholar Week 2016 - present
Pollinators play a key role in both ecosystem diversity and agricultural productivity [1]. Declines for pollinators have been seen across the globe and have been linked to agriculture and urbanization [2]. Several species in Illinois have declined so severely, they are now endangered or of significant conservation concern [1]. Past ONU students have collected data on the bees in Northeast IL. However, there is no data for what species are at the Sanctuary. This study will provide baseline data for future studies and by comparing with Midewin, a mature restoration, we can gain an understanding of distinctiveness.
Michigan's Upper Peninsula Is The Cradle Of Paul Bunyan's Beehives, Thomas J. Straka
Michigan's Upper Peninsula Is The Cradle Of Paul Bunyan's Beehives, Thomas J. Straka
Upper Country: A Journal of the Lake Superior Region
One of the Michigan Upper Peninsula’s earliest industries was iron production. Iron furnaces (smelters) were part of that industry; these were primarily fueled by charcoal, produced in hundreds of charcoal kilns scattered across the Upper Peninsula. The four basic kiln designs (rectangular, round, conical, and beehive) were all used in the region, with the beehive design becoming the predominant form. James C. Cameron, Jr. is credited with developing the beehive charcoal kiln design while employed by Upper Peninsula iron furnaces. This design was first introduced to the Upper Peninsula, and then to Northeastern Wisconsin, Northern New York, and the Far …
Annotation Of Hypothetical Genes In Lactococcus Lactis Ssp. Il403, Jennifer A. Tangires
Annotation Of Hypothetical Genes In Lactococcus Lactis Ssp. Il403, Jennifer A. Tangires
Student Scholar Showcase
The human gastrointestinal tract (GIT) harnesses various microbial organisms involved in almost all processes of physiological homeostasis, among these are lactic acid bacteria (LAB). These bacteria, almost all of which belong to the order Lactobacillales, are able to produce lactic acid, and play an important role in food preservation because they produce bacteriocins. Bacteriocins are antimicrobial proteins that are used to fight off related bacteria in their environment that are competing for the same resources. This study focuses on a specific LAB strain, Lactococcus lactis ssp. IL1403 where 21.9% of its predicted genes have not yet been assigned a function. …
Exploring Mesolimbic Circuitry Modulation By Opiates, Interleukin-10, And Psychostimulants, Joakim W. Ronström
Exploring Mesolimbic Circuitry Modulation By Opiates, Interleukin-10, And Psychostimulants, Joakim W. Ronström
Theses and Dissertations
The mesolimbic dopamine (DA) system originates in the ventral tegmental area (VTA) and projects to the nucleus accumbens (NAc) and other areas including the basolateral amygdala (BLA), prefrontal cortex, and the hippocampus. Drug use induces reward and leads to dysregulation in these brain areas and eventually to substance use disorders (SUDs). Chapter 1 introduces the mesolimbic DA system and its relationship to drug use and their relevance to each chapter. Chapter 2 explores opioid effects on BLA circuitry which is known to play a role in the emotional response including anxiety and stress in SUDs. We showed that morphine induced …
Synthesis Of Zntpp-Il As A Potential Photodynamic Therapy Agent, Addison White
Synthesis Of Zntpp-Il As A Potential Photodynamic Therapy Agent, Addison White
Honors Theses
Photodynamic Therapy is a new technique used in cancer treatment involving the use of a photosensitizer—a porphyrin—and light exposure to kill malignant cells. In this research, ZnTPP-IL was used as a photosensitizer with white light as a possible source of treatment of NIH 3T3 fibroblast and A549 lung cancer cell lines, with preliminary DNA testing on MYC-22 and G-quadruplex samples. ZnTPP-IL was synthesized by the reaction of the porphyrin ZnTPPC with isoleucinol. The new porphyrin was then purified by column chromatography using Sephadex LH-20 and G-50. ZnTPP-IL was characterized through NMR, IR, UV-vis, and fluorescence spectroscopies. The purity of the …