Life Sciences Commons^™

First Report Of Hog-Plum (Spondias Pinnata) Leaf Spot Disease, Kunal Mandal

Kunal Mandal

Hog–plum is a tree species with edible fruits. The plant is naturally distributed in the tropical areas of the Indian subcontinent. Our effort to introduce it in the semi– arid conditions of western India failed as the plants developed severe shot–hole type leaf spot symptoms. Association of a fungus with the disease was detected and its pathogenicity was established. The pathogen was identified to the genus level (Colletotrichum) based on the morphological and molecular markers.

Limnological Assistance For Lake Mead National Recreation Area: Quarterly Report, Period Ending December 31, 2011, Margaret N. Rees

Limnological Studies

Project 1

• Technical assistance with the implementation of the Interagency Monitoring Action Plan (IMAP) for Quagga Mussels is ongoing; remaining analyses are underway with report delivery rescheduled for 05/2012.
• Data have been delivered for Veliger abundance and settlement at different depths of Lake Mead with report delivery re-scheduled for 05/2012.
• Growth of adult quagga mussels within Las Vegas Wash/Las Vegas Bay
• The Interagency Quagga Mussel Meeting was held on 11/17/2011; the next meeting is scheduled for 02/16/2011.

Project 2

• Appendix 7, an addition to the Long-term Limnological and Aquatic Resource Monitoring and Research Plan for Lakes Mead and Mohave, has …

The Flora Of Greater San Quintín, Baja California, Mexico (2005–2010), Sula E. Vanderplank

Aliso: A Journal of Systematic and Floristic Botany

The plants of San Quintín (Baja California, Mexico) were documented through intensive fieldwork and collection of herbarium specimens to create a checklist of species. The region is located near the southernmost extent of the California Floristic Province, and the flora is influenced by the adjacent desert to the south. A total of 435 plant taxa were documented, of which 82% are native plants. Almost one in three native plant taxa in Greater San Quintín is a sensitive species, here documented as rare and/or locally endemic, and many taxa have yet to be evaluated fully for rarity and threats. Four major …

A New Variety Of Lomatium Ravenii (Apiaceae) From The Northern Great Basin And Adjacent Owyhee Region, Kimberly M. Carlson, Donald H. Mansfield, James F. Smith

Aliso: A Journal of Systematic and Floristic Botany

Variability in the group of Lomatium species comprising L. nevadense, L. ravenii, and L. foeniculaceum has led to conflicting classification schemes. While some taxonomists have treated L. ravenii as a distinct species made up of all the populations from California, Nevada, Idaho, and Oregon, others considered L. ravenii to be nothing more than a morphological extreme of L. nevadense. We examined morphological and phylogenetic data from across the range of L. ravenii, concluding that variation in the species warrants varietal distinction. Non-metric multidimensional scaling analysis of 29 populations shows two distinct groups—one from the vicinity of …

Cover Page

Aliso: A Journal of Systematic and Floristic Botany

No abstract provided.

Index

Aliso: A Journal of Systematic and Floristic Botany

No abstract provided.

Two Epiparasitic Species Of Phoradendron (Viscaceae) From Honduras: One New And For The Other A Range Extension And Host Determination, Delbert Wiens, Clyde L. Calvin

Aliso: A Journal of Systematic and Floristic Botany

A new species of Phoradendron, Ph. mathiasenii, is described, and a range extension and host are given for Ph. tikalense. Both species are from Honduras and grow as epiparasites, i.e., mistletoes parasitizing other mistletoes. The parasitic host for both epiparasitic species of Phoradendron is Psittacanthus angustifolius (Loranthaceae), which in turn is parasitic on the terrestrial host, Pinus oocarpa, a common forest tree in the collection area. Although the two species of Phoradendron parasitize the same parasitic host and co-occur in the area, they are distinct morphologically. Many plants of Ph. mathiasenii were observed at several collection …

Occurrence Of The Invasion Associated Marker (Iam) In Campylobacter Jejuni Isolated From Cattle, Yasser M. Sanad, Issmat I. Kassem, Zhe Liu, Jun Lin, Jeffrey T. Lejeune, Gireesh Rajashekara

Animal Science Publications and Other Works

Background

The invasion associated marker (iam) has been detected in the majority of invasive Campylobacter jejuni retrieved from humans. Furthermore, the detection of iam in C. jejuni isolated from two important hosts, humans and chickens, suggested a role for this marker in C. jejuni's colonization of multiple hosts. However, no data exist regarding the occurrence of this marker in C. jejuni isolated from non-poultry food-animals such as cattle, an increasingly important source for human infections. Since little is known about the genetics associated with C. jejuni's capability for colonizing physiologically disparate hosts, we investigated the occurrence …

Rediscovery Of Monotropastrum Sciaphilum (Andres) G.D.Wallace In China After 91 Years, Min Shen, Chang-Qin Zhang, Gary D. Wallace

Aliso: A Journal of Systematic and Floristic Botany

Monotropastrum sciaphilum (Ericaceae), a mycoheterotrophic member of subfamily Monotropoideae, was rediscovered at its type locality in Yunnan Province, China, 91 years after it was first collected. The type locality is the only locality from which it is known. Field observations in 2007–2010 indicated that inflorescences emerge from the soil between late April and early September. The restricted distribution of M. sciaphilum recommends its listing in the IUCN red book.

Enhanced Microbial Utilization Of Recalcitrant Cellulose By An Ex Vivo Cellulosome-Microbe Complex, Chun You, Xiao-Zhou Zhang, Noppadon Sathitsuksanoh, Lee R. Lynd

Dartmouth Scholarship

A cellulosome-microbe complex was assembled ex vivo on the surface of Bacillus subtilis displaying a miniscaffoldin that can bind with three dockerin-containing cellulase components: the endoglucanase Cel5, the processive endoglucanase Cel9, and the cellobiohydrolase Cel48. The hydrolysis performances of the synthetic cellulosome bound to living cells, the synthetic cellulosome, a noncomplexed cellulase mixture with the same catalytic components, and a commercial fungal enzyme mixture were investigated on low-accessibility recalcitrant Avicel and high accessibility regenerated amorphous cellulose (RAC). The cellbound cellulosome exhibited 4.5- and 2.3-fold-higher hydrolysis ability than cell-free cellulosome on Avicel and RAC, respectively. The cellulosome-microbe synergy was not completely …

Spt5 Affects The Rate Of Mrna Degradation And Physically Interacts With Ccr4 But Does Not Control Mrna Deadenylation, Yajun Cui, Yueh-Chin Chiang, Palaniswamy Viswanathan, Darren J. Lee, Clyde L. Denis

New Hampshire Agricultural Experiment Station Publications

The CCR4-NOT complex has been shown to have multiple roles in mRNA metabolism, including that of transcriptional elongation, mRNA transport, and nuclear exosome function, but the primary function of CCR4 and CAF1 is in the deadenylation and degradation of cytoplasmic mRNA. As previous genetic analysis supported an interaction between SPT5, known to be involved in transcriptional elongation, and that of CCR4, the physical association of SPT5 with CCR4 was examined. A two-hybrid screen utilizing the deadenylase domain of CCR4 as a bait identified SPT5 as a potential interacting protein. SPT5 at its physiological concentration was shown to immunoprecipitate CCR4 and …

Thermal Adaptation And Diversity In Tropical Ecosystems: Evidence From Cicadas (Hemiptera, Cicadidae), Allen F. Sanborn, James E. Heath, Polly K. Phillips, Maxine S. Heath, Fernando G. Noriega

Department of Biological Sciences

The latitudinal gradient in species diversity is a central problem in ecology. Expeditions covering approximately 16u549 of longitude and 21u49 of latitude and eight Argentine phytogeographic regions provided thermal adaptation data for 64 species of cicadas. We test whether species diversity relates to the diversity of thermal environments within a habitat. There are general patterns of the thermal response values decreasing in cooler floristic provinces and decreasing maximum potential temperature within a habitat except in tropical forest ecosystems. Vertical stratification of the plant communities leads to stratification in species using specific layers of the habitat. There is a decrease in …

Loss Of Activity Mutations In Phospholipase C Zeta Abloishes Calcium Oscillatory Ability Of Human Recombinant Protein In Mouse Ooctyes, J. Kashir, C. Jones, H.C. Lee, K. Rietdork, D. Nikiforaki, C. Durrans, M. Ruas, S.T. Tee, B. Heindryckx, A. Galione, P. Desutter, Rafael Fissore, J. Parrington, K. Coward

Rafael Fissore

BACKGROUND: Mammalian oocyte activation occurs via a series of intracellular calcium (Ca(2+)) oscillations thought to be induced by a sperm-specific phospholipase C zeta (PLCζ). There is now strong evidence to indicate that certain types of human male infertility are caused by failure of the sperm to activate the oocyte in an appropriate manner. Molecular analysis of the PLCζ gene of a male patient with oocyte activation deficiency has previously identified a point mutation causing a histidine to proline substitution at PLCζ residue 398 (PLCζ(H398P)), leading to abnormal Ca(2+) release profiles and reduced oocyte activation efficiency. METHODS AND RESULTS: In the …

Natural Variation In Gestational Cortisol Is Associated With Patterns Of Growth In Marmoset Monkeys (Callithrix Geoffroyi), Aaryn C. Mustoe, Andrew K. Birnie, Andrew V. Korgan, Jonathan Bruce Santo, Jeffrey French

Psychology Faculty Publications

High levels of prenatal cortisol have been previously reported to retard fetal growth. Although cortisol plays a pivotal role in prenatal maturation, heightened exposure to cortisol can result in lower body weights at birth, which have been shown to be associated with adult diseases like hypertension and cardiovascular disease. This study examines the relationship between natural variation in gestational cortisol and fetal and postnatal growth in marmoset monkeys. Urinary samples obtained during the mother’s gestation were analyzed for cortisol. Marmoset body mass index (BMI) was measured from birth through 540 days in 30- or 60-day intervals. Multi-level modeling was used …

Transcriptomic Profiles Of Peripheral White Blood Cells In Type Ii Diabetes And Racial Differences In Expression Profiles, Jinghe Mao, Junmei Ai, Xinchun Zhou, Ming Shenwu, Manuel Ong Jr., Marketta Blue, Jasmine T. Washington, Xiaonan Wang, Youping Deng

Faculty Publications

Background: Along with obesity, physical inactivity, and family history of metabolic disorders, African American ethnicity is a risk factor for type 2 diabetes (T2D) in the United States. However, little is known about the differences in gene expression and transcriptomic profiles of blood in T2D between African Americans (AA) and Caucasians (CAU), and microarray analysis of peripheral white blood cells (WBCs) from these two ethnic groups will facilitate our understanding of the underlying molecular mechanism in T2D and identify genetic biomarkers responsible for the disparities.

Results: A whole human genome oligomicroarray of peripheral WBCs was performed on 144 …

A Review Of Baobab (Adansonia Digitata) Products: Effect Of Processing Techniques, Medicinal Properties And Uses, Donatien Kabore, Hagrétou Sawadogo-Lingani, Bréhima Diawara, Clarise Compaoré, Mamoudou H. Dicko Prof., Mogens Jacobsen

Pr. Mamoudou H. DICKO, PhD

A general literature review including the effect of processing techniques, medicinal value and uses of baobab tree is reported in this manuscript. Baobab tree has multi-purpose uses, as it produces food and non-food products such as medicines, fuel, timber, fodder. Every part of the baobab tree is reported to be useful. The seeds, leaves, roots, flowers, fruit pulp and bark of baobab are edible. Baobab leaves are used in the preparation of soup. Seeds are used as a thickening agent in soups, but they can be fermented and used as a flavouring agent or roasted and eaten as snacks. The …

Morpholino Gene Knockdown In Adult Fundulus Heteroclitus: Role Of Sgk1 In Seawater Acclimation, Emily G. Notch, Joseph R. Shaw, Bonita A. Coutermarsh, Marisa Dzioba, Bruce A. Stanton

Dartmouth Scholarship

The Atlantic killifish (Fundulus heteroclitus) is an environmental sentinel organism used extensively for studies on environmental toxicants and salt (NaCl) homeostasis. Previous research in our laboratory has shown that rapid acclimation of killifish to seawater is mediated by trafficking of CFTR chloride channels from intracellular vesicles to the plasma membrane in the opercular membrane within the first hour in seawater, which enhances chloride secretion into seawater, thereby contributing to salt homeostasis. Acute transition to seawater is also marked by an increase in both mRNA and protein levels of serum glucocorticoid kinase 1 (SGK1) within 15 minutes of transfer. …

Pentachlorophenol Decreases Tumor-Cell-Binding Capacity And Cell-Surface Protein Expression Of Human Natural Killer Cells, Tasia Hurd, Jasmine Walker, Margaret M. Whalen

Chemistry Faculty Research

Pentachlorophenol (PCP) is an organochlorine pesticide that decreases the tumor-cell killing (lytic) function of human natural killer (NK) cells. NK cells defend against tumor cells and virally infected cells. They bind to these targets, utilizing a variety of cell-surface proteins. This study examined concentrations of PCP that decrease lytic function for alteration of NK binding to tumor targets. Levels of PCP that caused loss of binding function were then examined for effects on expression of cell-surface proteins needed for binding. Exposure to 10 µm PCP for 24 h (which caused a greater than 70% loss of lytic function) decreased NK …

A Shared Gene Expression Signature In Mouse Models Of Ebv-Associated And Non-Ebv-Associated Burkitt Lymphoma, Kathryn T. Bieging, Kamonwan Fish, Subbarao Bondada, Richard Longnecker

Microbiology, Immunology, and Molecular Genetics Faculty Publications

The link between EBV infection and Burkitt lymphoma (BL) is strong, but the mechanism underlying that link has been elusive. We have developed a mouse model for EBV-associated BL in which LMP2A, an EBV latency protein, and MYC are expressed in B cells. Our model has demonstrated the ability of LMP2A to accelerate tumor onset, increase spleen size, and bypass p53 inactivation. Here we describe the results of total gene expression analysis of tumor and pretumor B cells from our transgenic mouse model. Although we see many phenotypic differences and changes in gene expression in pretumor B cells, the transcriptional …

Identifying Characteristics Of Effective Small Group Learning Valued By Medical Students And Facilitators, Diana T. Robillard, Laura M. Spring, Susan J. Pasquale, Judith A. Savageau

Judith A. Savageau

Background: Small group teaching is an important part of undergraduate medical education, providing the ideal setting for learners to clarify misunderstandings, test hypotheses and evaluate ideas. Many schools undergoing curriculum reform have increased the time students spend in small group learning. However, there is an overall paucity of literature examining case-based small group sessions in medical school.

Objective: This study was designed to examine student and facilitator perceptions of effective case-based small group teaching in the pre-clinical years and compare results in order to identify similarities and differences and identify key areas of disconnect so that the small …

Improving Prenatal Education In A Health Center: A Pilot Study, Marcy Keddy Boucher, Hugh Silk, Judith A. Savageau, Katharine C. Barnard, Mary Flynn

Judith A. Savageau

Background: Currently, the timing and variety of health education topics that are covered during prenatal visits are decided upon by the individual physician caring for the patient. Consequently, some patients do not gain exposure to important subject matter that could potentially improve their satisfaction and pregnancy outcomes. Previously reported studies have found evidence that improved patient education may increase rates of breastfeeding (as well as duration), seat belt use during pregnancy, and decrease preterm low birth weight infants. In addition, one study has shown that when prenatal visits are targeted to specific objectives, the number of prenatal visits can …

Assessment Of Client Satisfaction In Six Urban Wic Clinics, Catherine Christie, Julia A. Watkins, Anita Martin, Helen Jackson, Judy E. Perkin, Jacquie Fraser

Catherine Christie

Customer service is a measure of support and courtesy provided to individuals who patronize an organization, and is a factor vital to the success of any business. Programs that strive to meet critical needs of at risk populations, such as the Special Supplementation Nutrition Program for Women, Infants, and Children (WIC), may also benefit from assessment of client satisfaction. The purpose of the study was to examine factors related to customer satisfaction in Duval County Florida WIC clinics and identify potential barriers to participation through a two-year project initiated by the health department and the state university. The study examined …

Public Health Practitioner Expert Opinion For Recommending Revisions To A Community Nutrition Mph Curriculum, Judy E. Perkin, Catherine Christie, Desiree Hayes

Catherine Christie

Soliciting practitioner input into the planning or revision of Master of Public Health programs is desirable to ensure that students are adequately prepared for public health practice. Members of the American Dietetic Association Public Health/Community Nutrition Dietetic Practice Group (ADA-PH/CNDPG) were surveyed regarding the structure of, as well as the knowledge and skills desirable for, inclusion in a Master of Public Health community nutrition program. A total of 998 surveys were mailed in June 2001 with a return rate of 34%. Approximately 73% of respondents indicated public health employment. The average response regarding optimal time for full-time degree completion was …

Effects Of Dairy Consumption On Sirt1 And Mitochondrial Biogenesis In Adipocytes And Muscle Cells, Antje Bruckbauer, Michael B. Zemel

Nutrition Publications and Other Works

Background

Recent data from this laboratory suggest that components of dairy foods may serve as activators of SIRT1 (Silent Information Regulator Transcript 1), and thereby participate in regulation of glucose and lipid metabolism. In this study, an ex-vivo/in-vitro approach was used to examine the integrated effects of dairy diets on SIRT1 activation in two key target tissues (adipose and muscle tissue).

Methods

Serum from overweight and obese subjects fed low or high dairy diets for 28 days was added to culture medium (similar to conditioned media) to treat cultured adipocytes and muscle cells for 48 hours.

Results

Treatment with high …

Reverse Protection Assay: A Tool To Analyze Transcriptional Rates From Individual Promoters, Yan O. Zubo, Victor V. Kusnetsov, Thomas Börner, Karsten Liere

Dartmouth Scholarship

Transcriptional activity of entire genes in chloroplasts is usually assayed by run-on analyses. To determine not only the overall intensity of transcription of a gene, but also the rate of transcription from a particular promoter, we created the Reverse RNase Protection Assay (RePro): in-organello run-on transcription coupled to RNase protection to define distinct transcript ends during transcription. We demonstrate successful application of RePro in plastid promoter analysis and transcript 3' end processing.

Cyp704b1 Is A Long-Chain Fatty Acid V-Hydroxylase Essential For Sporopollenin Synthesis In Pollen Of Arabidopsis, Anna A. Dobritsa, Jay Shrestha, Marc Morant, Franck Pinot, Michiyo Matsuno, Rob Swanson, Birger Lindberg Møller, Daphne Preuss

Rob Swanson

Sporopollenin is the major component of the outer pollen wall (exine). Fatty acid derivatives and phenolics are thought to be itsmonomeric building blocks, but the precise structure, biosynthetic route, and genetics of sporopollenin are poorly understood.Based on a phenotypic mutant screen in Arabidopsis (Arabidopsis thaliana), we identified a cytochrome P450, designatedCYP704B1, as being essential for exine development. CYP704B1 is expressed in the developing anthers. Mutations in CYP704B1result in impaired pollen walls that lack a normal exine layer and exhibit a characteristic striped surface, termed zebraphenotype. Heterologous expression of CYP704B1 in yeast cells demonstrated that it catalyzes v-hydroxylation of long-chainfatty acids, …

The Murine Caecal Microrna Signature Depends On The Presence Of The Endogenous Microbiota, Natasha Singh, Elize A. Shirdel, Levi Waldron, Regan-Heng Zhang, Igor Jurisica, Elena M. Comelli

Publications and Research

The intestinal messenger RNA expression signature is affected by the presence and compo-sition of the endogenous microbiota, with effects on host physiology. The intestine is also characterized by a distinctive micronome. However, it is not known if microbes also impact intestinal gene expression epigenetically. We investigated if the murine caecal microRNA expression signature depends on the presence of the microbiota, and the potential implica-tions of this interaction on intestinal barrier function. Three hundred and thirty four mi-croRNAs were detectable in the caecum of germ-free and conventional male mice and 16 were differentially expressed, with samples from the two groups clustering …

Design And Synthesis Of Cationic Steroid Antimicrobial Compounds, Synthesis Of Glycolipids Recognized By Natural Killer T Cells And Development Of Tlr-1, Tlr-6 Heterodimer Binders And Studies Of Their Immunology Activities, Yanshu Feng

Theses and Dissertations

Cationic steroid antimicrobial agents (CSAs) are a family of bile acid derivatives. These compounds are amphiphilic and mimic endogenous antimicrobial peptides. The antimicrobial activities of CSA-13 have been investigated and due to portent bactericidal activities and low toxicity, a large amount of CSA-13 is demanded for clinic trails and other antimicrobial applications. During our studies, we optimized the synthetic route of CSA-13, so that it can be prepared at the kilogram, even in tons scale. We investigated three routes and one of them is suitable for industry, because only recrystallization is needed in the synthesis. Natural killer T cells (NKT …

Septotemporal Variation In Theta Rhythm Dynamics: Effects Of Speed And Habituation, James Hinman

Master's Theses

Theta (6-12 Hz) field potentials and the synchronization (coherence) of these potentials present neural network indices of hippocampal physiology. Theta signals within the hippocampal formation may reflect alterations in sensorimotor integration, the flow of sensory input and/or distinct cognitive operations. While the power and coherence of theta signals vary across lamina within the septal hippocampus, limited information is available about variation in these indices across the septotemporal (long) or areal axis. The present study examined the relationship of locomotor speed to theta indices at CA1 and DG sites across the septotemporal axis as well as in the entorhinal cortex. Our …

Design Of A Tetracycline Operon Inducible System For The Control Of Vaccinia Virus Replication: Implications For Vaccine Development, Caitlin J. Hagen

Master's Theses

The use of vaccinia virus (VACV) as a vaccine resulted in the eradication of smallpox in 1979. Characteristics that contribute to the effectiveness of VACV as a vaccine and viral vector include its ability to elicit strong, long-lived humoral and cell-mediated immune responses as a live-replicating virus and to accept large inserts of DNA into its genome. However, adverse events associated with its use as the smallpox vaccine have constrained it from being more widely utilized in vaccines and therapies. We propose to improve the safety of VACV as a live-replicating vector by using elements of the tet operon to …