Life Sciences Commons^™

The Development Of A Primer Payload With Microparticles For Uti Pathogen Identification Using Polythymidine- Modified Lamp Primers In Droplet Lamp, Jonas Otoo

KGI Theses and Dissertations

Nucleic acid amplification tests (NAATs) are among the diagnostic tests with the highest sensitivity and specificity. However, they are more complex to develop than other diagnostic tests such as biochemical tests and lateral flow immunoassay tests. Polymerase chain reaction (PCR) is the gold standard for NAATs. PCR requires thermal cycling to achieve clonal amplification of the target pathogen DNA for diagnosis. Thermal cycling poses a challenge in the development of PCR diagnostics for point-of-care (POC) settings. Loop-mediated isothermal amplification (LAMP) offers an isothermal method for NAATs diagnostics. The advancement of the microfluidics field significantly enhances the development of LAMP diagnostics …

Challenge-Based Learning & Steam Curriculum, Diana Lockwood

The STEAM Journal

STEAM education is being integrated into elementary schools as a way to engage more students in creativity, hands-on learning, and problem-based learning also referred to as Challenge-Based-Learning (CBL). This article focuses on elementary educators’ curriculum design for STEAM and presenting students with open-ended questions phrased as a challenge as a way to raise student interest and achievement (DeJarnette, 2018; Hunter-Doniger, 2018). When students received challenges to solve, they felt more open to sharing their ideas since there was more than one potential right answer (DeJarnette, 2018; Drake, 2012). When implementing CBL, teachers act as facilitators using a constructivist approach as …

Using Computational Fluid Dynamics And Optical Sensor Technology To Scale Cell Culture Platforms, Mandar Makwana

KGI Theses and Dissertations

Different cell culture vessels ranging from micro scale to laboratory scale to commercial scale play critical role in upstream process development for biologics manufacturing. Based on the mode of operation, cell culture vessels have different hydrodynamic environments, making it challenging to scale. Integrated approaches using computational tools supported by experimental studies can overcome these challenges. Computational Fluid Dynamics (CFD) is one such tool that can simulate hydrodynamics within the cell culture vessels and can provide insights at macro and micro-scale. Accuracy of a CFD model significantly depends on the fluid model and assumptions. Traditionally, simple two-equation fluid models were developed …

Stem Education And Retention For Black Women Using High-Impact Practices: Historically Black Colleges And Universities Vs. Predominantly White Liberal Arts Colleges, Annette Njei

CMC Senior Theses

Black women are significantly underrepresented within the fields of science, technology, engineering, and mathematics (STEM). To address this, the Association of American Colleges & Universities crafted ten high-impact practices to increase student engagement and promote retention. This research paper examines how three specific high-impact practices (learning communities, mentoring, and undergraduate research experience) are utilized in STEM education.This research paper explores and compares the best high impact approaches that successfully teach and retain Black women within the various fields of STEM within the differing academic environments of historically Black colleges & universities ( HBCUs) and predominantly white liberal art colleges (PWLACs). …

Primer Payload System For Higher-Order Multiplex Lamp: Design And Development Of Unit Processes, Tochukwu Dubem Anyaduba

KGI Theses and Dissertations

Design and Development of Platforms for the Application of Loop-mediated Isothermal Nucleic Acid Amplification, LAMP, in the Diagnosis of Polymicrobial Diseases

Tochukwu Dubem Anyaduba, Travis Schlappi (PI)

For the past two decades, several isothermal nucleic acid amplification technologies have emerged. These are mostly in response to the need for robust molecular diagnostic tools amenable to point-of-care and limited-resource settings. Of these, loop-mediated isothermal amplification, LAMP, stands out as a highly specific and rapid alternative to the polymerase chain reaction, PCR. One of LAMP's significant characteristics involves using four essential and two loop (rate increasing) primers to recognize six to eight …

What Can We Learn About Research Narratives From Professional Storytellers?, Kim Wilkins, Helen Marshall

The STEAM Journal

This short note on practice reflects on how “research narrative” is a much-used, but misunderstood term. Compelling stories about our research are important: for public-facing communications and for academic tenure confirmation and promotion. They are also important for researchers to gain a clearer sense of their own vision and values in the research process: they are not just a communication skill, they’re a career skill. But often researchers in STEM disciplines do not have the practical skills to write stories. We draw on our own practice as creative writers to share some simple and effective methods to bring arts expertise …

Modeling Residence Time Distribution Of Chromatographic Perfusion Resin For Large Biopharmaceutical Molecules: A Computational Fluid Dynamic Study, Kevin Vehar

KGI Theses and Dissertations

The need for production processes of large biotherapeutic particles, such as virus-based particles and extracellular vesicles, has risen due to increased demand in the development of vaccinations, gene therapies, and cancer treatments. Liquid chromatography plays a significant role in the purification process and is routinely used with therapeutic protein production. However, performance with larger macromolecules is often inconsistent, and parameter estimation for process development can be extremely time- and resource-intensive. This thesis aimed to utilize advances in computational fluid dynamic (CFD) modeling to generate a first-principle model of the chromatographic process while minimizing model parameter estimation's physical resource demand. Specifically, …

Gravity-Drawing Flexible Silicone Filaments As Fiber Optics And Model Foldamers, Katherine Snell

CMC Senior Theses

Here, we present a method of gravity-drawing polydimethylsiloxane (PDMS) silicone fibers with application as fiber optics and as model foldamers. Beginning as a viscous liquid, PDMS is cured using heat until its measured viscosity reaches 4000 mPa•s. The semi-cured elastomer is then extruded through a tube furnace to produce thin (diameters on the order of hundred micrometers) filaments with scalable lengths. PDMS is biocompatible, gas-permeable, flexible, and hydrophobic. Additionally, the PDMS surface hydrophobicity can be modified via UV exposure, O2 plasma, and corona discharge. We demonstrate the patternibility (i.e patterns of hydrophobicity) of PDMS fibers, adding complexity to potential foldamer …

Third Voices Conference On Teaching Stem With Music, September 22-23, 2019, Lawrence M. Lesser

Journal of Humanistic Mathematics

The third annual VOICES (Virtual Ongoing Interdisciplinary Collaborations on Educating with Song; https://www.causeweb.org/voices/) conference will be held online September 22-23, 2019. Chaired by Tiffany Getty, this conference will explore the use of song to teach STEM (science, technology, engineering, and mathematics) at the postsecondary (or secondary) level.

Novel Low Shear 3d Bioreactor For The Scaled Production Of High Purity Human Mesenchymal Stem Cells, Andrew Burns

KGI Theses and Dissertations

Human mesenchymal stem cells are an ideal candidate for stem cell therapies. They have been researched since the 1960’s and can differentiate into many desired functional cell types without undergoing teratogenesis. However, higher yields are needed for a marketable, successful stem cell therapy. To accomplish this, cells will have to be cultured to expand them to therapeutically relevant dosages for multiple patients. Bioreactor production is an ideal method to solve this problem.

The aim of this thesis is to test and validate a novel bioreactor for the cultivation of human mesenchymal stem cells. In this work, we investigate a novel …

Prediction Of The Outcome In Cardiac Arrest Patients Undergoing Hypothermia Using Eeg Wavelet Entropy, Hana Moshirvaziri

CGU Theses & Dissertations

Cardiac arrest (CA) is the leading cause of death in the United States. Induction of hypothermia has been found to improve the functional recovery of CA patients after resuscitation. However, there is no clear guideline for the clinicians yet to determine the prognosis of the CA when patients are treated with hypothermia. The present work aimed at the development of a prognostic marker for the CA patients undergoing hypothermia. A quantitative measure of the complexity of Electroencephalogram (EEG) signals, called wavelet sub-band entropy, was employed to predict the patients’ outcomes. We hypothesized that the EEG signals of the patients who …

Can A Comprehensive Transition Plan To Barefoot Running Be The Solution To The Injury Epidemic In American Endurance Runners?, Michael A. Scarlett

CMC Senior Theses

Fossils belonging to the genus Homo, dating as far back as two million years ago, exhibit uniquely efficient features suggesting that early humans had evolved to become exceptional endurance runners. Although they did not have the cushion or stability-control features provided in our modern day running shoes, our early human ancestors experienced far less of the running-related injuries we experience today. The injury rate has been estimated as high as 90% annually for Americans training for a marathon and as high as 79% annually for all American endurance runners. There is an injury epidemic in conventionally shod populations that …

Voices: Conference On Teaching Stem With Music, September 27-28, 2017, Gregory J. Crowther

Journal of Humanistic Mathematics

This first-of-its-kind, online-only conference will explore the use of music to teach STEM (science, technology, engineering, and mathematics) at the college level (including AP courses). Presentations will be live-streamed from the conference website, https://www.causeweb.org/voices. Online registrations (for only $10 apiece!) will be accepted at the conference website until the conclusion of the conference on September 28, 2017.

Lessons For Life, Story Musgrave

The STEAM Journal

This piece explores the creation of character and talent through a wide diversity of education and experience.

Ecoscience + Art Initiative: Designing A New Paradigm For College Education, Scholarship, And Service, Changwoo Ahn

The STEAM Journal

The paper presents a new initiative, EcoScience + Art, which blooms at George Mason University. The creator explains the background, history, and recent activities of the initiative, and also introduces an on-going special project called “The Rain Project”, a student participatory project to design, construct, and monitor a green infrastructure (i.e., floating wetland) for sustainable stormwater management on campus. The special project is geared to design and present a new paradigm to integrate college education, scholarship, and service. The relevance of the initiative and the special project to STEAM education is discussed.

Beer And Brewing In German Culture: Bridging The Gaps Within Steam, John D. Sundquist

The STEAM Journal

A university-level course on science, history, and culture of beer and brewing offers students from a wide range of disciplines a unique opportunity to learn from each other. They gain an appreciation for STEAM and the interaction of a number of disciplines while examining a subject of growing interest. This paper provides a brief description of such a course and includes specific examples of ways in which students explore science, engineering, humanities and the arts, as these areas of research come together in the study of beer and brewing.

Implementing Green Roofs On Movie Theaters And Shopping Centers: Business Cases In Profitable Sustainability, Ryan J. Miller

Pomona Senior Theses

This thesis presents the business case for installing green roofs on movie theaters and shopping centers. These businesses can then derive increased profits from the environmental benefits of reduced energy use and increased stormwater retention. After presenting the basic design and benefits of a green roof, the thesis develops stand-alone business plans for a movie theater and shopping center. The author finds that green roofs are a profitable sustainability solution for the commercial enterprise.

Towards A “Cloud Curriculum” In Art And Science?, Roger Malina

The STEAM Journal

Recently an email hit my desk from Paul Thomas in Australia with a proposal to work together on a “Cloud Curriculum for Art and Science”. I immediately agreed to collaborate. I don’t yet have a clue of what a cloud curriculum is, but what I do know is that we are ‘backing into the future’ in educational institutions and we desperately need a ‘cloud curriculum.’ We need to look over the ten year horizon. And in the emerging art-science field I doubt that the usual approach to curriculum development will work.

A Letter To The Steam Journal Readers, Deborah Freund

The STEAM Journal

A letter to the readers from the President of Claremont Graduate University, President Deborah Freund, welcoming the inaugural issue of 'The STEAM Journal'.

A Dna Computer For Glioblastoma Multiforme Diagnosis And Drug Delivery, Sumaiya F. Hashmi

CMC Senior Theses

Glioblastoma multiforme (GBM) is a debilitating malignant brain tumor with expected patient survival of less than a year and limited responsiveness to most treatments, often requiring biopsy for diagnosis and invasive surgery for treatment. We propose a DNA computer system, consisting of input, computation, and output components, for diagnosis and treatment. The input component will detect the presence of three GBM biomarkers: vascular endothelial growth factor (VEGF), caveolin-1α (CAV), and B2 receptors. The computation component will include indicator segments for each of these genes, and ensure that output is only released if all the biomarkers are present. The output component …

Sediment Removal From The San Gabriel Mountains, Mary C. Ferguson

Pitzer Senior Theses

The issue of sediment removal from the San Gabriel Mountains has been a complex issue that has created problems with beach replenishment, habitat destruction and the need to spend millions of dollars at regular intervals to avoid safety hazards. Most recently 11 acres of riparian habitat, including 179 oaks and 70 sycamores, were removed for sediment placement. Other sites including Hahamongna Watershed Park and La Tuna Canyon also face a similar fate. This thesis questions: How did we get to this point of destroying habitat to dump sediment which is viewed as waste product? What are the barriers for creating …

Effect Of Substrate Composition And Alignment On Corneal Cell Phenotype, Donna Phu '09, Lindsay S. Wray '08, Robert V. Warren '10, Richard C. Haskell, Elizabeth J. Orwin

All HMC Faculty Publications and Research

Corneal blindness is a significant problem treated primarily by corneal transplants. Donor tissue supply is low, creating a growing need for an alternative. A tissue-engineered cornea made from patient-derived cells and biopolymer scaffold materials would be widely accessible to all patients and would alleviate the need for donor sources. Previous work in this lab led to a method for electrospinning type I collagen scaffolds for culturing corneal fibroblasts ex vivo that mimics the microenvironment in the native cornea. This electrospun scaffold is composed of small-diameter, aligned collagen fibers. In this study, we investigate the effect of scaffold nanostructure and composition …

Immunogold Labeling To Enhance Contrast In Optical Coherence Microscopy Of Tissue Engineered Corneal Constructs, Chris B. Raub, Elizabeth J. Orwin, Richard C. Haskell

All HMC Faculty Publications and Research

Our lab has used an optical coherence microscope (OCM) to assess both the structure of tissue-engineered corneal constructs and their transparency. Currently, we are not able to resolve cells versus collagen matrix material in the images produced. We would like to distinguish cells in order to determine if they are viable while growing in culture and also if they are significantly contributing to the light scattering in the tissue. In order to do this, we are currently investigating the use of immunogold labeling. Gold nanoparticles are high scatterers and can create contrast in images. We have conjugated gold nanoparticles to …