Life Sciences Commons^™

Characterization And Selection Of Hop Cultivars Adapted To Nebraska, Kristina Alas

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Hop (Humulus lupulus L.) is an ingredient in the beer brewing industry that provides beer its flavor and aroma. High demand from the brewing industry has encouraged production outside of the traditional Pacific Northwest, the primary production region. Producers in the Midwest are attempting to grow cultivars adapted to the Pacific Northwest, but environmental differences have caused low yields and changes in secondary metabolite content. To aid producers, a regional breeding program was initiated to develop cultivars adapted to the Midwest. Success of any breeding program relies on the selection of genetically superior parents to generate progeny with the …

Dicamba Tank Mixtures And Formulations And Their Effects On Sensitive Crops During Cleanout Procedures, Vinicius Velho

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The introduction of dicamba-tolerant (DT) soybeans (Glycine max L. Merr) and cotton (Gossypium hirsutum L) in 2017 provided an additional tool for herbicide resistant weeds management. In the subsequent years, off-target movement of dicamba allegedly caused damage to sensitive crops and vegetation.

Possible causes of off-target movement include tank contamination, physical drift, and volatility. Additional products, such as herbicides to control grass, are often added to tank with dicamba, which is used to control broadleaf weeds, to increase the spectrum of control and application efficiency. Dicamba products registered for DT crops require the use of drift reducing …

Prediction Of Cultivation Areas For The Commercial And An Early Flowering Wild Accession Of Salvia Hispanica L. In The United States, Mohammad Hassani, Thomas Piechota, Hagop S. Atamian

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Salvia hispanica L., commonly known as chia, is a plant-based alternative to seafood and is rich in heart-healthy omega-3 fatty acid, protein, fiber, and antioxidants. In the Northern Hemisphere, chia flowering is triggered by the fall equinox (12-h light and dark, early October) and the seeds mature after approximately three months. Chia is sensitive to frost and end of season moisture which limits its cultivation to small areas in regions with temperate climate. The U.S. chia import has increased considerably over the years; however, chia is not widely cultivated in the United States. This study used the historical U.S. temperature …

Increases In Vein Length Compensate For Leaf Area Lost To Lobing In Grapevine, Zoë Migicovsky, Joel F. Swift, Zachary Helget, Laura L. Klein, Anh Ly, Matthew Maimaitiyiming, Karoline Woodhouse, Anne Fennell, Misha Kwasniewski, Allison J. Miller, Peter Cousins, Daniel H. Chitwood

Agronomy, Horticulture and Plant Science Faculty Publications

Premise:Leaf lobing and leaf size vary considerably across and within species,including among grapevines (Vitisspp.), some of the best‐studied leaves. Weexamined the relationship between leaf lobing and leaf area across grapevinepopulations that varied in extent of leaf lobing.
Methods:We used homologous landmarking techniques to measure 2632 leavesacross 2 years in 476 unique, genetically distinct grapevines fromfive biparentalcrosses that vary primarily in the extent of lobing. We determined to what extent leafarea explained variation in lobing, vein length, and vein to blade ratio.
Results:Although lobing was the primary source of variation in shape across theleaves we measured, leaf area varied only slightly …

Increased Signal-To-Noise Ratios Within Experimental Field Trials By Regressing Spatially Distributed Soil Properties As Principal Components, Jeffrey C. Berry, Mingsheng Qi, Balasaheb V. Sonawane, Amy Sheflin, Asaph Cousins, Jessica Prenni, Daniel P. Schachtman, Peng Liu, Rebecca S. Bart

Department of Agronomy and Horticulture: Faculty Publications

Environmental variability poses a major challenge to any field study. Researchers attempt to mitigate this challenge through replication. Thus, the ability to detect experimental signals is deter-mined by the degree of replication and the amount of environmental variation, noise, within the experimental system. A major source of noise in field studies comes from the natural heterogeneity of soil properties which create microtreatments throughout the field. In addition, the variation within different soil properties is often nonrandomly distributed across a field. We explore this challenge through a sorghum field trial dataset with accompanying plant, microbiome, and soil property data. Diverse sorghum …

Expression Of Atwri1 And Atdgat1 During Soybean Embryo Development Influences Oil And Carbohydrate Metabolism, Cintia Lucía Arias, Truyen Quach, Tu Huynh, Hanh Nguyen, Ademar Moretti, Yu Shi, Ming Guo, Amira Rasoul, Kyujung Van, Leah Mchale, Thomas E. Clemente, Ana Paula Alonso, Chi Zhang

Department of Agronomy and Horticulture: Faculty Publications

Soybean oil is one of the most consumed vegetable oils worldwide. Genetic improvement of its concentration in seeds has been historically pursued due to its direct association with its market value. Engineering attempts aiming to increase soybean seed oil presented different degrees of success that varied with the genetic design and the specific variety considered. Understanding the embryo’s responses to the genetic modifications introduced, is a critical step to successful approaches. In this work, the metabolic and transcriptional responses to AtWRI1 and AtDGAT1 expression in soybean seeds were evaluated. AtWRI1 is a master regulator of fatty acid (FA) biosynthesis, and …

Neuropilin-2 Regulates Androgen-Receptor Transcriptional Activity In Advanced Prostate Cancer, Samikshan Dutta, Navatha Shree Polavaram, Ridwan Islam, Sreyashi Bhattacharya, Sanika Bodas, Thomas Mayr, Sohini Roy, Sophie Alvarez, Marieta I. Toma, Anza Darehshouri, Angelika Borkowetz, Stefanie Conrad, Susanne Fuessel, Manfred Wirth, Gustavo B. Baretton, Paramita Ghosh, Kenneth J. Pienta, David L. Klinkebiel, Surinder K. Batra, Michael H. Muders, Kaustubh Datta

Department of Agronomy and Horticulture: Faculty Publications

Aberrant transcriptional activity of androgen receptor (AR) is one of the dominant mechanisms for developing of castration-resistant prostate cancer (CRPC). Analyzing AR-transcriptional complex related to CRPC is therefore important towards understanding the mechanism of therapy-resistance. While studying its mechanism, we observed that a transmembrane protein called neuropilin-2 (NRP2) plays a contributory role in forming a novel AR-transcriptional complex containing nuclear pore proteins. Using immunogold electron microscopy, high-resolution confocal microscopy, chromatin immunoprecipitation, proteomics, and other biochemical techniques, we delineated the molecular mechanism of how a specific splice variant of NRP2 becomes sumoylated upon ligand stimulation and translocates to the inner nuclear …

Contribución A La Conservación De La Orquídea Rodriguezia Granadensis (Lindl.) Rchb.F. En La Hacienda Betania (Fusagasugá, Colombia), Arlette Ivonne Gil Clavijo, Jenny Paola Moreno Lopez, Laguandio Del Cristo Banda Sánchez

Ciencias Agropecuarias

Esta cartilla, creada por el Grupo de Investigación PROSAFIS de la Universidad de Cundinamarca, presenta los resultados del proyecto Aproximación a la ecología de orquídeas nativas en el ecosistema hacienda Betania con proyección a su conservación, manejo y producción. Se destaca la orquídea Rodriguezia granadensis, adaptada a forofitos (phorophytes) de cítricos y guayabos, representando el 83,3 % de los individuos observados. Predominó la fase vegetativa (fenología) durante todo el período, con más floración y cápsulas en épocas lluviosas, sincronizando su fase reproductiva con el régimen de lluvias. En las raíces se encontró el hongo endófito (endophyte) Rhizoctonia en …

Identification Of Putative Snp Markers Associated With Resistance To Egyptian Loose Smut Race(S) In Spring Barley, Kamal A.M. Abo-Elyousr, Amira M.I. Mourad, P. Stephen Baenziger, Abdelaal H.A. Shehata, Peter E. Eckstein, Aaron D. Beattie, Ahmed Sallam

Department of Agronomy and Horticulture: Faculty Publications

Loose smut (LS) disease is a serious problem that affects barley yield. Breeding of resistant cultivars and identifying new genes controlling LS has received very little attention. Therefore, it is important to understand the genetic basis of LS control in order to genetically improve LS resistance. To address this challenge, a set of 57 highly diverse barley genotypes were inoculated with Egyptian loose smut race(s) and the infected seeds/plants were evaluated in two growing seasons. Loose smut resistance (%) was scored on each genotype. High genetic variation was found among all tested genotypes indicating considerable differences in LS resistance that …

Sorghum Association Panel Whole-Genome Sequencing Establishes Cornerstone Resource For Dissecting Genomic Diversity, J. Lucas Boatwright, Sirjan Sapkota, Hongyu Jin, James C. Schnable, Zachary Brenton, Richard Boyles, Stephen Kresovich

Department of Agronomy and Horticulture: Faculty Publications

Association mapping panels represent foundational resources for understanding the genetic basis of phenotypic diversity and serve to advance plant breeding by exploring genetic variation across diverse accessions. We report the whole-genome sequencing (WGS) of 400 sorghum (Sorghum bicolor (L.) Moench) accessions from the Sorghum Association Panel (SAP) at an average coverage of 38× (25–72×), enabling the development of a high-density genomic marker set of 43 983 694 variants including single-nucleotide polymorphisms (approximately 38 million), insertions/deletions (indels) (approximately 5 million), and copy number variants (CNVs) (approximately 170 000). We observe slightly more deletions among indels and a much higher prevalence …

Medicinal Plant Use For Type-2 Diabetes Treatment In The Traditional Vietnamese Medicine System Of Southern Vietnam, Lan Truong

Dissertations, Theses, and Capstone Projects

This ethnobotanical study examines the traditional health care system of the Mekong River Delta in Vietnam. The basic inquiry focuses on how the healers in the traditional Vietnamese medicine (TVM) system are treating a disease relatively new in Vietnam, type-2 diabetes (T2D). The study includes a description of the system in which the healers work. The research design compares two locations, one on the mainland (Rạch Giá City) and the other on a nearby island (Phú Quốc Island).

There are six chapters. The first chapter establishes foundational information about the region, population, and the traditional medical system. The results of …

Seed Longevity And Climatic Tolerance Of San Joaquin Wooly-Threads (Monolopia Congdonii; Asteraceae) An Endangered Plant From The San Joaquin Desert, California, Paul Excoffier

Master's Theses

San Joaquin wooly-threads (Monolopia congdonii; Asteraceae) is a federally-listed, endangered annual plant species from the desert areas of the San Joaquin Valley. Its limited range puts it at risk of extinction if the climate changes in such a way as to hinder its growth and reproduction. The primary aims of the study were to 1) determine how long-lived the seeds of the M. congdonii are, a key determinant of survival of desert annual plant populations through long droughts and 2) determine how severely hotter, drier conditions impact the ability of emerged plants to grow and reproduce. Secondarily, I …

Reassessment Of Species Boundaries And Phylogenetic Relationships In The Desmodium Ciliare Complex (Fabaceae) Using Morphological And Dna Data, Joshua Wilkinson

Master's Theses

Desmodium (Beggar's ticks) is a generally weedy genus of approximately 280 species in the angiosperm family Fabaceae (Subfamily Papilionoideae, Tribe Desmodieae) characterized by indehiscent loments constricted into segments. Within the Southeastern U.S., the Desmodium ciliare group is one of two Desmodium species complexes that have been historically difficult, with much argument about the limits of species and the role of hybridization. There are three commonly recognized species within the D. ciliare group, D. ciliare (Muhl. ex Willd.) DC., Desmodium marilandicum (L.) DC., and D. obtusum (Muhl. ex Willd.) DC., with two supposed hybrids in addition to the …

Climatic Influences On Summer Use Of Winter Precipitation By Trees, Gregory R. Goldsmith, Scott T. Allen, Sabine Braun, Rolf T. W. Siegwolf, James W. Kirchner

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Trees in seasonal climates may use water originating from both winter and summer precipitation. However, the seasonal origins of water used by trees have not been systematically studied. We used stable isotopes of water to compare the seasonal origins of water found in three common tree species across 24 Swiss forest sites sampled in two different years. Water from winter precipitation was observed in trees at most sites, even at the peak of summer, although the relative representation of seasonal sources differed by species. However, the representation of winter precipitation in trees decreased with site mean annual precipitation in both …

Lawrence Kaplan (14 April 1926-6 March 1918), Emily Kaplan

Andean Past

This is an appreciation of the life and work of archaeobotanist Lawrence Kaplan, a specialist in domesticated beans.

A Small Stem Assay For Chestnut Blight Resistance In Hybrid American Chestnut Trees, Evelyn Odle

Honors Theses

Cryphonectria parasitica (Murr.) Barr is the causal agent for the chestnut blight disease, which was brought to North America in the late 19th century on nursery stock from Japan (Anagnostakis 1987). A pandemic of chestnut blight lasted for the next half century, nearly wiping out all American chestnut trees (Castanea dentata Borkh.). Restoration works to restoring the American chestnut to its native range in North America’s canopy has been going on for the past century. The American Chestnut Foundation has strived to breed a resistant American chestnut by backcross breeding with Chinese species (C. mollissima Blume) since the 1980s. The …

Investigating The Mechanics And Chemistry Of Sickle Polish Development, Justin Jared Dubois

Graduate Theses and Dissertations

This series of experiments is designed to understand the development and chemistry of sickle gloss. Sickles are common in the archaeological record and have long been studied for their eponymous “sickle gloss”. There is debate as to how this gloss is formed. Five experimental sickles containing flint and novaculite blades were used to harvest high and low moisture content rye and common fescue and associated field weeds. The differences in the development of sickle gloss were examined. High moisture content plants create thick, smooth fields of polish with undulating, billowing margins at a higher developmental speed. Low moisture content plants …

Climate Change And Conservation Of Milkweed: Evidence Of Extensive Admixture Between Common Milkweed And Poke Milkweed, Elizabeth Davies

Undergraduate Honors Theses

Global climate change can drive many changes in species interactions. One primary way it affects species is by changing climates, causing species to expand their ranges and allowing them to interact with species from whom they were previously isolated. In plants, new species interactions can result in hybridization – the creation of hybrid offspring between two separate species. This hybridization can increase gene flow between the species and lead to introgression, the transfer of genetic material from one species to another through hybrid backcrossing with the parent species. My thesis investigates hybridization in the model system Asclepias (milkweed) by analyzing …

Investigating The Effect Of Longhorned Milkweed Beetle Larval Root Feeding On Resprouting In Common Milkweed, Margaret Donnan

Undergraduate Honors Theses

Clonal reproduction is an important form of reproduction for many plants, and plants can grow clonally through a variety of methods, such as belowground stems, specialized organs, or adventitious buds on their roots (Herben and Klimešová 2020). In plants that reproduce via buds on their roots, researchers have shown that damage to roots can stimulate bud production and sprouting (Bartušková and Klimešová 2010). Common milkweed (Asclepias syriaca) is a root- sprouting perennial plant that receives yearly damage to its roots via the feeding of the larvae of the specialist longhorned milkweed beetle (Tetraopes tetrophthalmus) (Erwin et al. 2013). In this …

Regional Plant Community Differences In The Nebraska Sandhills, Travis Millikan

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The Nebraska Sandhills is very valuable to the state of Nebraska, representing one of the most in-tact and largest grassland ecosystems in temperate regions in the world. Rangeland managers must understand plant community dynamics across the Sandhills to better inform management decisions. The first objective of this study was to evaluate plant community variability on upland Sands ecological sites across different precipitation zones in the Nebraska Sandhills. The second objective of our study was to utilize the Rangeland Analysis Platform (RAP) to examine spatial and temporal variability in biomass production and cover on pastures of ranches analyzed in the first …

Integration Of Medicinal Fungi Into The Heber W. Youngken Jr. Medicinal Garden, Anthony Makashov

Senior Honors Projects

The Heber W. Youngken Jr. Medicinal Garden is a one-of-a-kind resource that houses close to 300 medicinal plants and is filled to the brim with educational opportunities. For the College of Pharmacy, this garden has been the centerpiece of many classes taught here at the University of Rhode Island and has roots as a physical teaching space. While the garden contains a plethora of plant species, medicinal fungi have yet to have been included in the garden, despite their relevance as both sources of pharmaceutical drug discovery and as natural products with inherent physiological benefits themselves. The purpose of my …

Temporal Dynamics And Seed Dispersal In Plant-Frugivore Communities Of The Dominican Republic, Spencer Christian Schubert

Biological Sciences Theses & Dissertations

Plant-animal mutualisms are a foundational component of biodiversity in terrestrial ecosystems. Most tropical forest plants have adapted to produce fleshy fruits to attract frugivorous animals to disperse seeds. Interaction patterns among plant taxa and their seed dispersers are driven by a complex suite of factors involving their evolutionary history and environmental context, and the structure of these mutualistic networks are theoretically tied to their ecological function. I carried out a series of field studies to investigate the temporal dynamics of mutualistic interactions of plant and avian frugivore communities in the central Dominican Republic and how their characteristics affect seed dispersal …

Native And Non-Native Ant Impacts On Soil Microbes, Hannah A. Stewart

Biology Theses

Organisms produce chemical weapons for defense, but target organisms can develop resistance. In their introduced range, non-native species may bring “novel weapons” against which native organisms have not co-evolved resistance. The invasive European fire ant (Myrmica rubra) may have brought antimicrobial secretions to the Northeastern United States that are novel weapons against native fungal and bacterial soil organisms. I hypothesized that M. rubra would better inhibit seed pathogens resulting in greater emergence of native myrmecochorous Viola sororia seeds and, as a side effect, more strongly inhibit arbuscular mycorrhizal fungi than a native seed dispersing ant (Aphaenogaster picea …

Chromosome-Level Thlaspi Arvense Genome Provides New Tools For Translational Research And For A Newly Domesticated Cash Cover Crop Of The Cooler Climates, Adam Nunn, Isaac Rodríguez-Arévalo, Zenith Tandukar, Katherine Anna Frels, Adrián Contreras-Garrido, Pablo Carbonell-Bejerano, Panpan Zhang, Daniela Ramos Cruz, Katharina Jandrasits, Christa Lanz, Anthony Brusa, Marie Mirouze, Kevin Dorn, David W. Galbraith, Brice A. Jarvis, John C. Sedbrook, Donald L. Wyse, Christian Otto, David Langenberger, Peter F. Stadler, Detlef Weigel, M. David Marks, James A. Anderson, Claude Becker, Ratan Chopra

Department of Agronomy and Horticulture: Faculty Publications

Thlaspi arvense (field pennycress) is being domesticated as a winter annual oilseed crop capable of improving ecosystems and intensifying agricultural productivity without increasing land use. It is a selfing diploid with a short life cycle and is amenable to genetic manipulations, making it an accessible field-based model species for genetics and epigenetics. The availability of a high-quality reference genome is vital for understanding pennycress physiology and for clarifying its evolutionary history within the Brassicaceae. Here, we present a chromosome-level genome assembly of var. MN106-Ref with improved gene annotation and use it to investigate gene structure differences between two accessions (MN108 …

Exploration Of Genes Controlling Grain Yield Heterosis In Hybrid Wheat (Triticum Aestivum L.) Utilizing 3ʹ Rna Sequencing, Nichole Miller

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The implementation and future success of hybrid wheat (Triticum aestivum L.) is impacted by breeders’ inability to create consistent high yielding, high heterosis hybrids. This research addresses this problem by conducting an exploration of transcriptomes from hybrids and parent lines to determine what genes are active in heterotic or non-heterotic hybrids and how their level of expression can explain the phenotype of grain yield heterosis. Using hybrids that showed positive mid-parent heterosis (MPH), classified as heterotic in our study, and negative or no difference MPH hybrids, classified as non-heterotic, differentially expressed genes (DEGs) potentially related to heterosis and hybrid …

Agricultural Intensification Can Help Protect The Amazon Forest And Reduce Global Warming / Protecting The Amazon Forest And Reducing Global Warming Via Agricultural Intensification, Fabio R. Marin, Alencar J. Zanon, Juan P. Monzon, José F. Andrade, Evandro H.F.M. Silva, Gean L. Richter, Luis A.S. Antolin, Bruna S.M.R. Ribeiro, Giovanna G. Ribas, Rafael Battisti, Alexandre B. Heinemann, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

The Amazon basin includes 550 M ha covered with rainforests, with 60% of this area being in Brazil. Conversion of rainforest for soybean production raises concerns about the degree to which Brazil can reconcile production and environmental goals. Here we investigated the degree to which intensification could help Brazil produce more soybean without further encroachment of the Amazon Forest. Our analysis shows that continuation of current trends in soybean yield and area would lead to conversion of additional 5.7 M ha of forests and savannas during the next 15 years, with an associated 2550 Mt of CO2eq released into the …

Hybrid Incompatibilities In Metrosideros, Jacyln Martinez

Undergraduate Research Symposium Podium Presentations

Prediction: Postzygotic reproductive isolating barriers, relating to hybrid incompatibilities are contributing to the speciation of Metrosideros

Climate Change And Management Impacts On Soybean N Fixation, Soil N Mineralization, N2O Emissions, And Seed Yield, Elvis F. Elli, Ignacio A. Ciampitti, Michael J. Castellano, Larry C. Purcell, Seth Naeve, Patricio Grassini, Nicolas C. La Menza, Luiz Moro Rosso, André F. De Borja Reis, Péter Kovács, Sotirios V. Archontoulis

Department of Agronomy and Horticulture: Faculty Publications

Limited knowledge about how nitrogen (N) dynamics are affected by climate change, weather variability, and crop management is a major barrier to improving the productivity and environmental performance of soybean-based cropping systems. To fill this knowledge gap, we created a systems understanding of agroecosystem N dynamics and quantified the impact of controllable (management) and uncontrollable (weather, climate) factors on N fluxes and soybean yields. We performed a simulation experiment across 10 soybean production environments in the United States using the Agricultural Production Systems sIMulator (APSIM) model and future climate projections from five global circulation models. Climate change (2020–2080) increased N …

Evaluating Traits Influencing Hybrid Wheat Seed Production Using A Double Haploid Population Derived From Freeman X Camelot, Emre Karahan

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Hybrid wheat (Triticum aestivum L.) cultivars are preferred for their advanced agronomic traits such as a yield improvement increase up to 20%. However, hybrid cultivars remain difficult to produce on a commercial scale due to inadequate pollen dispersal, the closed nature of the wheat floret and the high cost of inducing sterility or restoring fertility in cleistogamous wheat. Redesigning parent-specific traits and understanding their potential interactions with related agronomic traits are needed to breed improved male lines in hybrid seed production fields. To better understand the genetic control of anther extrusion (AE), 174 double haploid lines derived from Freeman …

Two Fingerprinting Sets For Humulus Lupulus Based On Kasp And Microsatellite Markers, Mandie Driskill, Katie Pardee, Kim E. Hummer, Jason D. Zurni, Keenan L. Amundsen, Annette Wiles, Claudia Wiedowi, Josef Patza, John A. Henningi, Nahla V. Bassili

Department of Agronomy and Horticulture: Faculty Publications

Verification of clonal identity of hop (Humulus lupulus L.) cultivars within breeding programs and germplasm collections is vital to conserving genetic resources. Accurate and economic DNA-based tools are needed in dioecious hop to confirm identity and parentage, neither of which can be reliably determined from morphological observations. In this study, we developed two fingerprinting sets for hop: a 9-SSR fingerprinting set containing high-core repeats that can be run in a single PCR reaction and a kompetitive allele specific PCR (KASP) assay of 25 single nucleotide polymorphisms (SNPs). The SSR set contains a sex-linked primer pair, HI-AGA7, that was used …