Life Sciences Commons^™

Teaching Evolutionary Principles With Ai Image Generators, Jacqueline Garnett

Generative AI Teaching Activities

The purpose of this assignment is for students to practice constructing phylogenies using the principle of parsimony. In this activity, students will use an AI Image Generator to create a grid of images of the same organism and identify a series of traits that vary to build a possible phylogenetic tree.

Investigation Of The Growth Of Listeria In Plant-Based Beverages, Klaudia Bartula, Sambou Biagui, Máire Begley, Michael Callanan

Department of Biological Sciences Publications

The objective of the present study was to evaluate whether the content of sugar, protein, fat, or fibre in commercially available and specially formulated plant-based beverages (oat, soya and pea) influences the growth rates of Listeria. Beverages were inoculated with a strain cocktail of Listeria (approximately 1 × 103 CFU/mL), and the data demonstrated that Listeria could proliferate in all tested beverages. Moreover, varying concentrations of naturally occurring or added sugar (0–3.3%), protein (3.3–5%), fat (1.1–3.5%) and added fibre (0–1.5%) did not have a statistically significant (p > 0.05) impact on the growth rates of Listeria in the tested …

Transcriptional Dynamics During Rhodococcus Erythropolis Infection With Phage Wc1, Dana Willner, Sudip Paudel, Andrew D. Halleran, Grace E. Solini, Veronica Gray, Margaret Saha

Arts & Sciences Articles

Background

Belonging to the Actinobacteria phylum, members of the Rhodococcus genus thrive in soil, water, and even intracellularly. While most species are non-pathogenic, several cause respiratory disease in animals and, more rarely, in humans. Over 100 phages that infect Rhodococcus species have been isolated but despite their importance for Rhodococcus ecology and biotechnology applications, little is known regarding the molecular genetic interactions between phage and host during infection. To address this need, we report RNA-Seq analysis of a novel Rhodococcus erythopolis phage, WC1, analyzing both the phage and host transcriptome at various stages throughout the infection process.

Results

By five …

Adherent-Invasive Escherichia Coli (Aiec) Promotes Mild Colitis In The C57bl/6 Il-10 Ko Mouse Model Of Crohn's Disease, Sarah Sm Zaw

Senior Theses

Crohn's disease (CD) is a chronic inflammatory bowel disease. Interleukin-10 knockout (IL-10 KO) mice are crucial in CD research. Adherent-invasive Escherichia coli (AIEC) is a subgroup of E. coli. In one study, IL-10 KO mice colonized with AIEC developed colitis in the distal colon compared to wild-type (WT) mice. I investigated how AIEC NC101 WT affects colitis severity in IL-10 KO mice of the C57BL/6 genetic background. Similar results in both genotypes would enhance mouse strain accessibility. Germ-free C57BL6 IL-10 KO mice were divided into groups: colonized with AIEC NC101 for 12, 15, or 18 weeks, or kept germ-free. Histopathological …

Bioaerosol Size As A Potential Determinant Of Airborne E. Coli Viability Under Ultraviolet Germicidal Irradiation And Ozone Disinfection, Weixing Hao, Yue-Wern Huang, Yang Wang

Biological Sciences Faculty Research & Creative Works

Ultraviolet germicidal irradiation (UVGI) and ozone disinfection are crucial methods for mitigating the airborne transmission of pathogenic microorganisms in high-risk settings, particularly with the emergence of respiratory viral pathogens such as SARS-CoV-2 and avian influenza viruses. This study quantitatively investigates the influence of UVGI and ozone on the viability of E. coli in bioaerosols, with a particular focus on how E. coli viability depends on the size of the bioaerosols, a critical factor that determines deposition patterns within the human respiratory system and the evolution of bioaerosols in indoor environments. This study used a controlled small-scale laboratory chamber where E. …

How To Spark Bio Curiosity: An Innovative High School Biology Curriculum, Audrie Charles

Graduate Research Showcase

This project is a researched creative endeavor focused around the creation of an exemplary high school biology course. This presentation will showcase an original high school biology curriculum designed to ignite students’ innate curiosity and empower meaningful learning of core concepts like cell structure, genetics, evolution and ecology. Students revisit key ideas through a spiral curriculum that builds complexity, tackling open-ended problems and collaborating on hands-on inquiries like formulating hypotheses, designing experiments, collecting data and drawing evidence-based conclusions. Tangible projects allow students to demonstrate content knowledge in addition to critical thinking abilities like synthesizing concepts, evaluating claims and applying learning …

Quantifying Costs And Benefits Of Alternative Overwintering Strategies In A Southeastern Population Of Timber Rattlesnakes (Crotalus Horridus), John Powers, Amber Stubbs, Alfred J. Mead, Dominic L. Desantis

Graduate Research Showcase

Temperate reptile populations are under strong selective pressure to minimize the costs of extreme winter temperatures. For high latitude and high elevation snake populations, this has often favored communal overwintering behavior, wherein large numbers of conspecifics aggregate at optimal sites given the limited availability of such features in the environment. Timber Rattlesnakes (Crotalus horridus) are distributed across a wide latitudinal gradient in North America, and therefore represent ideal models for exploring how variable winter selective regimes might favor different strategies. Here, we leverage a unique intermediate population of C. horridus from central Georgia, USA, that exhibits both communal …

Development Of A Novel Biology Laboratory Course For Incarcerated Students, Elsa H.K. Spencer, Joanna R. Vondrasek

Inquiry: The Journal of the Virginia Community Colleges

Federal second chance Pell grants were recently made widely available to incarcerated students in the United States to fund undergraduate education. Piedmont Virginia Community College was a pilot site for this expansion and began full scale implementation of transfer-oriented associate degrees at three correctional centers in its service area. The college did not have a biology laboratory curriculum that would meet general education requirements and be feasible in a correctional center, where students do not have internet access or even reliable computer (word processing or spreadsheet) access and where materials are restricted for entry. This paper will serve to introduce …

An Analysis Of Novel Yeasts And Their Brewing Potential, Hannah Murray

Research Honors

Within the brewing community, there is a race to discover new ways to brew beers to create new flavor and smell profiles. Scientists across the globe are searching for novel species of yeasts which have the potential to enhance the flavor and smell of their beer. While most beers are brewed using one of a handful of domesticated yeast strains -- also known as pitching yeasts -- finding “wild” yeasts is one way to change fermentation products. Experimentation has been started on this front due to the wide array of properties that a wild yeast can change in a brew. …

Varecia Rubra (Red Ruffed Lemur) Diel Activity And Calling At Andranobe, Masoala National Park, Madagascar, Hannah Elena Hilden-Reid

Dissertations and Theses

With respect to the natural variation in abiotic and biotic conditions present between daytime and nighttime periods, many animal species show evolutionary adaptations specialized for diurnality, nocturnality, or crepuscularity. Biologists have traditionally viewed categorizations of this kind as fixed within taxonomic groups, emphasizing how intricately species’ survival is contingent on the ability to adapt to low light, high light, or twilight conditions. However, movement away from such distinct temporal niche categories began within chronobiological studies following the discovery of more flexible patterns of activity in numerous taxa during the late 1970s. This shift generated increased interest in elucidating the complex …

The Effects Of Land Use Change On Ant Communities In New England, Amelia K.L. Curry, Joseph A. Nelsen, Dale R. Stevens, Kaitlyn A. Mathis

Biology

Urbanisation and agricultural expansion are two of the most prevalent and intense forms of land use change worldwide and can have dramatic consequences on biodiversity and biotic community structure. Ants are extremely widespread, ecologically diverse and small ectotherms that are sensitive to changes from a wide range of environmental factors. Therefore, ants make an ideal study organism to examine the effects of anthropogenic land use change on biotic communities. In this study, we examined differences in ant species richness and community composition between urban parks, farms and forest fragments, and related these differences to environmental factors that vary among each …

Exploration Of Cytotoxicity Of _Rabidosa Rabida_ Venom On Invertebrate And Vertebrate Cell Cultures, Martina Gooden

Honors Theses

Venom has been observed in thousands of species across different taxa. It can be adapted for different purposes, including prey capture or predator defense. Venom is found almost universally in spiders, but there has been little exploration of spiders that are not medically significant. Rabidosa rabida, the rabid wolf spider, is a common species whose venom has not been adequately investigated. Previous studies indicate that they do not require venom to apprehend easy prey in a laboratory setting, raising a question as to what their venom is adapted to do. To evaluate its effect on mammalian cells, L929 murine fibroblasts …

Ecological Niche Modeling Of Endemic Texas Crayfish Species Under Current Conditions, Sarah A. Burgett

Biology Theses

Crayfish play many important roles in freshwater ecosystems such as ecosystem engineering, bioindicator, and food resource. Even though they are important to ecosystems, they are highly understudied. There has been previous work done on ecological niche modeling on Texas crayfish species, but this study takes it a step further by combining soil, climate, and hydrology variables to model the ecological niches of three endemic species: Procambarus texanus, Procambarus nueces, and Procambarus fayettei. Ecological niche models were created for the three species of interest using the program Maxent. I found that P. texanus and P. fayettei have indistinguishable …

Effects Of Sex, Cadmium, And High-Fat-Diet On Accumulation Of Essential Metals In Mouse Kidneys., Justin Y. Xu, Adam Gaweda, Michelle Barati, Michael Merchant, Jun Cai, Lu Cai, Madhavi Rane

Undergraduate Research Events

Effects of cadmium and obesity on accumulation of essential metals in mouse kidney were evaluated. Starting in utero and continuing until 24 weeks postweaning, male/female mice were exposed to cadmium (0, 0.5, or 5 ppm) and were fed on Normal Diet (ND)/High-Fat Diet (HFD) post weaning. ICP-MS analysis was performed to evaluate cadmium and essential metals absorbed in the kidneys of aforementioned mice. Metals data obtained was analyzed using MANOVA with multiple comparisons (Bonferroni correction with p

Old Vs. New: An Experiment On The Efficacy Of Complexity-Focused Reproductive Biology Lectures For Undergraduate Biology Students, Ive N. Muse, Shraddha Patel, Linda Fuselier

Undergraduate Research Events

Undergraduate reproductive biology courses are traditionally taught in a reductive manner, prioritizing a binary understanding of sex which oversimplifies reproductive behaviors and reproductive system development across diverse taxa. Although this binary approach to lessons on reproduction is intended to benefit students in learning foundational human reproductive concepts, it does not teach the most recent research in reproductive biology, which identifies reproductive variation as the norm. We designed and implemented a lesson on reproductive biology informed by the most current and accurate research and compared it to a traditionally taught biology lecture for first year majors. Students who received the experimental …

Did God Create Viruses?, James Hayward

Faculty Publications

No abstract provided.

Effects Of Wind Turbine Noise On Songbird Behavior During Nonbreeding Season, Yael Lehnardt, Jesse R. Barber, Oded Berger-Tal

Biology Faculty Publications and Presentations

Anthropogenic noise is one of the fastest growing, globally widespread pollutants, affecting countless species worldwide. Despite accumulating evidence of the negative impacts of wind turbines on wildlife, little is known about how the noise they generate affects ecological systems. Songbirds may be susceptible to noise pollution due to their reliance on vocal communication and thus, in this field study, we examined how songbirds are affected by wind turbine noise. We broadcasted noise produced by one wind turbine in a migratory stopover site during the nonbreeding season. Throughout the study, we repeatedly monitored the acoustic environment and songbird community before, during, …

Effect Of Lunar Magnetic Field And Lunar Regolith Simulant On The Growth And Bioactive Compounds Production Of Chlorella Vulgaris Microalgae, Jeries Philip Butros Abedrabbo

Mechanical & Aerospace Engineering Theses & Dissertations

Since humans last went to the moon on the Apollo missions, there has been a fascination with inhabiting and colonizing other planetary objects, starting with the Earth’s Moon, Mars, and recently Jupiter’s moon, Europa. However, there is still lack of knowledge and science behind many of the extraterrestrial environmental effects on biological organisms living on Earth. Therefore, there is a need to study how such environments would affect these organisms. Moreover, how can we attain sustainable living in such environments as well through self-providing life support systems (LSS), without the need to provide for additional necessities back and forth from …

Parasite Diversity Of The King Cobra (Ophiophagus Hannah), Indian Rat Snake (Ptyas Mucosa), And Spectacled Cobra (Naja Naja), Bailey Tator

ALL - Honors Theses

Thus far, there has been little research on the parasitic biodiversity in wild reptiles, particularly in India. Research has been done on the parasite diversity of certain species, such as the King cobra (Ophiophagus hannah) and the Spectacled cobra (Naja naja), however these samples were purely from captive animals (Rajesh, 2015). This gap in knowledge is important to address because of how critical parasites are to the ecosystem and understanding their host population (Hudson, 2006). Parasites are some of the most biodiverse organisms, yet there is little work done to describe them in biodiversity hotspots such as the Western Ghats …

Pulsed Electric Fields Sensitize Methicillin-Resistant Staphylococcus Aureus To Antibacterial Therapies And Stimulate Host Immune Responses, Alexandra E. Chittams-Miles

Biomedical Sciences Theses & Dissertations

This research explores the impact of nanosecond pulsed electric fields (nsPEF) on two fronts: their immune stimulatory effects and their potential as a novel strategy to enhance the sensitivity of Methicillin-resistant Staphylococcus aureus (MRSA) to clinically relevant antibiotics. While pulsed electric fields have been reported to have an immune stimulatory effect, the mechanisms responsible for these effects have yet to be determined.

Our investigation addresses the rising concern of MRSA derived skin and soft tissue infections (SSTIs). Consistent with other publications, we found that nsPEF alone cause modest inactivation of planktonic MRSA. We then investigated the effects of nsPEF in …

Climate Change-Associated Declines In Water Clarity Impair Feeding By Common Loons, Walter H. Piper, Max R. Glines, Kevin C. Rose

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Climate change has myriad impacts on ecosystems, but the mechanisms by which it affects individual species can be difficult to pinpoint. One strategy to discover such mechanisms is to identify a specific ecological factor related to survival or reproduction and determine how that factor is affected by climate. Here we used Landsat imagery to calculate water clarity for 127 lakes in northern Wisconsin from 1995 to 2021 and thus investigate the effect of clarity on the body condition of an aquatic visual predator, the common loon (Gavia immer). In addition, we examined rainfall and temperature as potential predictors …

Genomic Analysis Of Seven Mycobacteriophages Identifies Three Novel Species With Differing Phenotypic Stabilities, Laura O'Connell, Aidan Coffey Prof., Jim O'Mahony

Department of Biological Sciences Publications

Recently, case studies have been published regarding the application of mycobacteriophage (MP) therapy (MPT) in patients with multi-antibiotic-resistant infections. A major limitation in the development of MPT is the paucity of therapeutically useful MP. As there are approximately 10,000 MP that have yet to be sequenced, it is possible that characterization of this cohort would increase the repertoire of useful MP. This study aims to contribute to such a strategy, by characterizing a cohort of 7 mycobacteriophages. Sequencing analyses revealed that the MP have unique sequences, and subsequent gene annotation revealed differences in gene organization. Notably, MP LOCARD has the …

Biological Significances Of Environmental Pollution In The New Calabar River, Southern Nigeria: A Case Study In Zooplankton Composition, Ibienebo Davies Imachrist, Francis David Sikoki, Philomina Kika, Davies Ibienebo Chris

Journal of Bioresource Management

The study was conducted for three months (May to July 2020), focusing on the physicochemical parameters and zooplankton composition in the Ogbogoro region of the New Calabar River in Nigeria. This investigation was carried out at three distinct monitoring stations. The physicochemical parameters were determined using standard ecological analytic procedures. PAST and SPSS were used for the statistical analysis. The temperature ranged between 26.5 to 33 ºC, salinity (1.23 to 1.86 %), pH (7.4 to 9.62), Total Dissolved Solids (2419 to 5,817 mg/L), conductivity (223.83 to 7,328 μS/cm), turbidity (3.415 to 15.05 NTU) and Dissolved Oxygen (3.8 to 6.8 mg/L). …

Generating Crispr Mutations In The Asymmetric Leaves1 Gene Of Arabidopsis, Gabriella J. Rueschhoff, Tara Phelps-Durr

SACAD: John Heinrichs Scholarly and Creative Activity Days

The goal of this study is to create new mutations in the ASYMMETRIC LEAVES 1 (AS1) gene of Arabidopsis using CRISPR technology. AS1 is a myb domain transcription factor that represses the KNOX genes, a group of genes involved in maintaining an undifferentiated cell state. AS1 binds to the chromatin remodeling protein Histone Repressor A (HIRA). In animals, HIRA is involved in the permanent suppression of proliferation genes required for early development. The interaction between AS1 and HIRA provides clues to how AS1 maintains suppression at the KNOX genes; however, we must be careful applying what …

Trip13’S Crucial Role In Pancreatic Cancer Progression, Swati Dhasmana, Anupam Dhasmana, Stella Rios, Iris A. Enriquez-Perez, Sheema Khan, Farrukh Afaq, Upender Manne, Murali M. Yallapu, Subhash Chauhan

Research Symposium

Background: Pancreatic cancer, characterized by its high mortality rate, stands as one of the most aggressive cancer forms. The projected surge in pancreatic cancer-related deaths, making it the second leading cause in the United States by 2030, underscores the urgency for effective early screening tools. This study employs data mining methods to scrutinize bioinformatic data surrounding TRIP13. Examining differential expression across various cancers, correlating TRIP13 expression with pancreatic cancer stages, exploring associations with common cancer genes, and analyzing overall survival rates constitute the core investigations. Integrated with molecular biology techniques, the study further quantifies TRIP13 expression in progressive pancreatic cancer …

Ongoing Study Of The Association Of Apoe Gene Polymorphisms With Cognitive Impairment In The Rio Grande Valley Hispanic Population, Jaime Miguel L. Saveron, Daniela Gamez, Chun Xu

Research Symposium

Background: Alzheimer's disease (AD), a prevalent neurodegenerative disorder, exhibits a significant genetic component. In addition, mild cognitive impairment (MCI) is a key risk factor for AD. While certain demographic factors and genetic variants associated with AD and cognitive impairment have been identified in non-Hispanic populations, limited research exists on this subject within the Hispanic population, particularly in the Rio Grande Valley. This study aims to investigate the relationship between the APOE gene, specifically its ε4 allele, and cognitive impairment in the Hispanic population of the Rio Grande Valley.

Methods: A total of 269 Hispanic subjects, aged 60 and above, with …

A Tick's Quest: The Effects Of Climatic Stress On Host-Seeking Behavior Of Wild Ticks, Lawson Trimmell, Peter Briggs, Javier Monzón

Seaver College Research And Scholarly Achievement Symposium

The Lone Star Tick (Amblyomma americanum) is one of the most epidemiologically relevant tick species in the United States and is experiencing a range expansion. Our study aimed to investigate the effects of climatic stress on the behavior and physiology of the Lone Star Tick at the expansion front. We hypothesized that there are differences in survivorship, questing (host-seeking) behavior, and dehydration tolerance based on sex or geographic origin. We also predicted that ticks increase their host-seeking behavior as they approach death due to dehydration stress. We collected adult ticks from six sites across Oklahoma and New York/New Jersey. We …

Effectiveness Of Frog Skin Secretions Against Uv-B Radiation, Connor Mcgaha, Kinkade Mcmurray, Lee B. Kats

Seaver College Research And Scholarly Achievement Symposium

As an increasing number of their species face possible extinction, amphibian populations have been steadily declining over the past decades (IUCN). While various factors have been suggested or proven to be partially responsible for this reduction, increased ultraviolet B radiation (UVBR) appears to be one of the more prominent explanations, especially when considering the continual thinning of the Earth’s ozone layer due to anthropogenic climate change. However, it has also been discovered that skin secretions, which are reported to function as a component of amphibians’ innate immune systems, may have an effect on UVBR (Cramp and Franklin). With this in …

Deep Learning Can Be Used To Classify And Segment Plant Cell Types In Xylem Tissue, Reem Al Dabagh, Benjamin Shin, Sean Wu, Fabien Scalzo, Helen Holmlund, Jessica Lee, Chris Ghim, Samuel Fitzgerald, Marinna Grijalva

Seaver College Research And Scholarly Achievement Symposium

Studies of plant anatomical traits are essential for understanding plant physiological adaptations to stressful environments. For example, shrubs in the chaparral ecosystem of southern California have adapted various xylem anatomical traits that help them survive drought and freezing. Previous studies have shown that xylem conduits with a narrow diameter allows certain chaparral shrub species to survive temperatures as low as -12 C. Other studies have shown that increased cell wall thickness of fibers surrounding xylem vessels improves resistance to water stress-induced embolism formation. Historically, these studies on xylem anatomical traits have relied on hand measurements of cells in light micrographs, …

The Role Of Sirt7 In Beta Cell Function And Insulin Secretion, Trevor C. Kendrick, Jeffrey S. Tessem, Connor Littlefield

Library/Life Sciences Undergraduate Poster Competition 2024

Diabetes is a chronic metabolic disease characterized by an inability of beta cells to produce or secrete insulin due to decreasing beta cell mass, a condition induced by beta cell death or overuse. Current treatment consists of daily administration of insulin to diabetic individuals. We have shown that Sirtuin 7 (Sirt7), a deacetylase located in the nucleus, directly interacts with Nkx6.1, a transcription factor essential for beta cell function and proliferation. We have shown that one of the post translational modifications that impinges on Nkx6.1 activity is acetylation. Given Sirt7’s role as a deacetylase, and published reports demonstrating its impact …