Biomaterials Commons^™

Evaluating Cellular Response Of Different Valve Interstitial Cell (Vic) Phenotypes To Angiotensin I, Smit Patel

Biomedical Engineering Undergraduate Honors Theses

Calcific Aortic Valve Disease (CAVD), one of the leading causes of death in the United States, is characterized by diminished functioning and limited movement of the aortic valve (AV) due to increased thickening, disorganized extracellular matrix elastin fibers, increased collagen content, and abnormal deposition and formation of calcium nodules on the AV [1, 2]. There are two types of CAVD: i) Aortic valve sclerosis (AVS), in which patients suffer from tissue hardening, fibrosis, and early calcification, and ii) calcific aortic stenosis (CAS), representative by excessive calcification on the AV and reduced AV opening, are the two main forms of CAVD …

Developing Aligned Nerve Scaffolds In A 3d Type-I Collagen Gel, Gabriel David

Biomedical Engineering Undergraduate Honors Theses

Despite significant progress in the field of peripheral nerve repair, clinical success is still limited, leaving millions to suffer from peripheral neuropathy with billions spent every year for treatment. Nerve repair methods that are capable of maximizing the regenerative properties of peripheral nerves are greatly desired in the field of medical science. This research aims to fill the gap between modern methods and the future of nerve repair by creating type-I collagen scaffolds with aligned degradation pores that will assist and nurture nerves growing through them. This is achieved by incorporating adipose stem cells into type-I collagen hydrogels and aligning …

Investigating Virus Clearance Via Ph Inactivation During Biomanufacturing, Wenbo Xu

Biomedical Engineering Undergraduate Honors Theses

In the processing of biopharmaceuticals, viral clearance and viral safety are important for the development of monoclonal antibodies. Murine xenotropic leukemia virus (XMuLV) is one of the retroviruses, recommended by Food and Drug Administration (FDA) as a model virus for viral clearance via inactivation from therapeutics derived from Chinese hamster ovary cells (CHO). A robust and effective method was investigated to clear or inactivate endogenous viruses by low pH inactivation. The effects of different conductivity and inactivated time on XMuLV clearance was determined. Acetate buffer was prepared with different conductivity, and 2% XMuLV was spiked into acetate buffer. XMuLV virus …

Analysis Of Biological Response To Ecm Hydrogel Injection, Grady Dunlap

Biomedical Engineering Undergraduate Honors Theses

Abstract

Under normal circumstances, skeletal muscle possesses the capacity to regenerate and heal via inflammatory and myogenic pathways. In cases of severe tissue loss or certain diseases, this capacity is lost, often resulting in loss of tissue function. Extracellular matrix (ECM), the protein scaffold which houses cells in physiological tissue, has been shown to have structural and chemical properties which influence cell migration and phenotype. This results in ECM’s capacity to encourage a regenerative response when implanted into severely damaged skeletal muscle. Additional advantages are apparent when an ECM scaffold is digested into a hydrogel, namely less invasive implantation via …

Isolation Of Metallic Single-Walled Carbon Nanotubes For Electrically Conductive Tissue Engineering Scaffolds, Jakob Hockman

Biomedical Engineering Undergraduate Honors Theses

Metallic single-walled carbon nanotubes (m-SWNTs) were separated from pristine SWNTs using affinity chromatography for use in electrically conductive tissue engineering scaffolds. Approximately one third of SWNTs have metallic properties. Separations were achieved using a protocol modified from Liu & coworkers (2011) in order to improve the method for cell culture environments. Samples enriched in m-SWNTs were isolated and characterized. However, challenges still remain for the complete separation of m-SWNTs from their semiconducting counterpart (s-SWNTs) using this protocol. Approaches to improve separation and reduce the difficulties associated with processing the nanotubes were suggested. One of the ultimate destinations of these nanotubes …