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2006

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Full-Text Articles in Biological Engineering

Department Of Biological Systems Engineering Newsletter, Issue 2, November 2006 Nov 2006

Department Of Biological Systems Engineering Newsletter, Issue 2, November 2006

BSE Department Magazine

Contents:
Visiting Scholar
Big changes in the BSE Computer Lab
From the Department Head
National Science Foundation Initiative Moving to Transform Water and Environment Research ByWayneWoldt
ASABE Awards
Nebraska Tractor Test Lab Welcomes New Director
Alumni News
August Graduation
College of Agricultural Sciences and Natural Resources Alumni Association (CASNRAA)
Hydrologic Studies in Prairie and Agricultural Watersheds Gleanings from a Faculty Development Leave by Dean E. Eisenhauer
Irrigation and Energy Conservation Field Day By Suat Irmak
P3–Ten Years
Hall of Fame
Vegetative Treatment Systems Help Small Livestock Producers Control Animal Waste by Kalee Olson
Ice Cream Social & Scholarship Recognition
Student …


Incorporation Of Polyethylene Glycol Into Self-Assembled Monolayers Enhances Substrate-Mediated Gene Delivery By Nonspecifically- Bound Complexes, Angela K. Pannier, Lonnie D Shea Nov 2006

Incorporation Of Polyethylene Glycol Into Self-Assembled Monolayers Enhances Substrate-Mediated Gene Delivery By Nonspecifically- Bound Complexes, Angela K. Pannier, Lonnie D Shea

Department of Biological Systems Engineering: Conference Presentations and White Papers

Developing systems capable of controlled and efficient gene transfer is a fundamental goal of biotechnology, with applications including functional genomics, gene therapy, and tissue engineering. Substrate-mediated delivery, also termed solid phase delivery, describes the immobilization of DNA, complexed with nonviral vectors, to a biomaterial or substrate through specific or nonspecific interactions. Cells cultured on the substrate are exposed to elevated DNA concentrations within the local microenvironment, which enhances transfection. We investigated transfection resulting from DNA complexes immobilized to a substrate through specific interactions introduced through complementary functional groups on the vector and surface or through nonspecific interactions. Self-assembled monolayers (SAMs) …


Closure Of Earthern Manure Structures (Including Basins, Holding Ponds And Lagoons), Don D. Jones, Richard K. Koelsch, Saqib Mukhtar, Ronald Sheffield, John W. Worley Aug 2006

Closure Of Earthern Manure Structures (Including Basins, Holding Ponds And Lagoons), Don D. Jones, Richard K. Koelsch, Saqib Mukhtar, Ronald Sheffield, John W. Worley

Department of Biological Systems Engineering: Conference Presentations and White Papers

This paper is a summary of what is known scientifically about the closure of earthen manure structures without artificial liners, including lagoons, storage basins, and runoff holding ponds, and what needs to be examined further to increase our understanding of the dynamics of closing them in an environmentally safe manner. This information should be useful as a guide for state regulatory agencies considering rules for closure and for academicians and consultants who work with livestock production facilities.


Department Of Biological Systems Engineering Newsletter, Issue 1, August 2006 Aug 2006

Department Of Biological Systems Engineering Newsletter, Issue 1, August 2006

BSE Department Magazine

Contents:
Welcome Comments from Department Head
Student Poster Exhibition
Quarter-scale Tractor Team
Distinguished Fellowship Awards
Scoops and Scholars
Educational Objectives
ASABE Honors Department Faculty and Staff Members
Computer Tool Helps Crop Production under Limited Irrigation
Comings and Goings
Milestones
Faculty and Staff News
Alumni Update
Alumni Profile
Graduation
UCare Spring 2006 Undergraduate Research
MSYM Student on Winning Team
A Nebraskan Goes Way South
Visitors to BSE


Vegetative Treatment Systems For Open Lot Runoff: Review Of Literature, Richard K. Koelsch, Jeffrey Lorimer, Kyle Mankin Aug 2006

Vegetative Treatment Systems For Open Lot Runoff: Review Of Literature, Richard K. Koelsch, Jeffrey Lorimer, Kyle Mankin

Department of Biological Systems Engineering: Conference Presentations and White Papers

Runoff from open lot livestock systems (beef and dairy) defined as Concentrated Animal Feeding Operations (CAFO) must be controlled by systems designed and managed to prevent the release of manure-contaminated runoff for storms equal to or less than a 25-year, 24-hour design storm. This performance standard has been attained for open lot systems with some combination of clean water diversion, settling basins, runoff collection ponds and irrigation systems (baseline system).

An alternative approach is to rely on overland flow and infiltration into cropland with perennial forage or grasses for treatment of open lot runoff. Such vegetative systems have been researched …


Yield Response Of Corn To Deficit Irrigation In A Semiarid Climate, José O. Payero, Steven R. Melvin, Suat Irmak, David D. Tarkalson Jul 2006

Yield Response Of Corn To Deficit Irrigation In A Semiarid Climate, José O. Payero, Steven R. Melvin, Suat Irmak, David D. Tarkalson

Biological Systems Engineering: Papers and Publications

Irrigation water supplies are decreasing in many areas of the U.S. Great Plains, which is requiring many farmers to con¬sider deficit-irrigating corn (Zea mays L.) or growing crops like winter wheat (Triticum aestivum L.) that require less water, but that are less profitable. The objectives of this study were to: (1) quantify the yield response of corn to deficit irrigation, and (2) determine which of several seasonal water variables correlated best to corn yield in a semiarid climate. Eight (T1–T8) and nine (T1–T9) deficit-irrigated treatments (including dryland), were compared in 2003 and 2004 in North Platte, Nebraska. The …


Economics Of Manure Phosphorus Distribution From Beef Feeding Operations, William F. Kissinger, Raymond E. Massey, Richard K. Koelsch, Galen E. Erickson Jul 2006

Economics Of Manure Phosphorus Distribution From Beef Feeding Operations, William F. Kissinger, Raymond E. Massey, Richard K. Koelsch, Galen E. Erickson

Department of Biological Systems Engineering: Conference Presentations and White Papers

An economic model was developed to evaluate cost and value of manure distribution. Feedlots ranging in size from 2,500 head to 25,000 head one-time capacities were used as case studies to calculate excretion amounts from cattle fed diets with a range of phosphorus. Diet P and subsequent costs of distributing the manure were used to analyze the corresponding costs of manure distribution, in addition to determining the required available land needed to be in compliance with a nutrient management plan based on utilization of manure P by the crops grown.

The model illustrated when animals are fed diets of increasing …


National Livestock And Poultry Environmental Learning Center, Richard K. Koelsch, Mark Risse, Joe Harrison, Jill Heemstra, Frank Humenik Jul 2006

National Livestock And Poultry Environmental Learning Center, Richard K. Koelsch, Mark Risse, Joe Harrison, Jill Heemstra, Frank Humenik

Department of Biological Systems Engineering: Conference Presentations and White Papers

This paper describes a new national initiative to improve the delivery of science-based information to non-research customers. The vision of the Livestock and Poultry Environmental Learning Center is to provide individuals involved in public policy issues, animal production, and delivery of technical services for confined animal systems with on-demand access to the nation’s best science-based resources that is responsive to priority and emerging environmental issues associated with animal agriculture. This paper describes the implementation plans for this project.


Application Of Aspen Mads-Box Genes To Alter Reproduction And Development In Trees, Gopi Krishna Podila, Leland James Cseke, Banalata Sen, David F. Karnosky Jun 2006

Application Of Aspen Mads-Box Genes To Alter Reproduction And Development In Trees, Gopi Krishna Podila, Leland James Cseke, Banalata Sen, David F. Karnosky

Michigan Tech Patents

The present invention provides compositions and methods for producing a transgenic plant that exhibits altered characteristics resulting from over expression or under expression of a novel polypeptide PtM3 or its homolog PtM4. The altered characteristics resulting from over-expression include at least one of the ability to convert axillary mer- istem to floral meristem; to accelerate flowering i.e., early flowering; to increase fruit production; to increase nut production; to increase seed output; to increase branching; to increase flower production; to increase fruit yield; to increase flower yield and a combination thereof. The altered characteristics resulting from suppressed expression include at least …


Possibilities Of Glutamate-Mediated Synaptic Like Activity In Somatosensory Mechanoreceptors Such As Pacinian Corpuscles?, Kevin Kopko May 2006

Possibilities Of Glutamate-Mediated Synaptic Like Activity In Somatosensory Mechanoreceptors Such As Pacinian Corpuscles?, Kevin Kopko

Honors Capstone Projects - All

Pacinian Corpuscles (PCs) are somatosensory mechanoreceptors, composed of a central neurite, surrounded by layers of lamellae that make up the inner core, which are specialized glial cells, and fibroblast derived outer-core lamellae of the surrounding capsule. Several components specific to a chemical synapse (Vglut1, Vamp1, SNAP-23) were localized to both the neurite and the inner-core cells in feline mesenteric PCs using immunocytochemistry. However, it has long been postulated that the first synapse of the PC occurs at the dorsal column nuclei, or spinal cord, and that no classical synapse is present in the distal ending of the capsulated receptor. While …


Transfected Cell Arrays For Assessment Of Estrogen Receptor Activation In Breast Cancer Cells, Angela K. Pannier, Zain Bengali, Eric A. Ariazi, V. Craig Jordan, Lonnie D Shea May 2006

Transfected Cell Arrays For Assessment Of Estrogen Receptor Activation In Breast Cancer Cells, Angela K. Pannier, Zain Bengali, Eric A. Ariazi, V. Craig Jordan, Lonnie D Shea

Department of Biological Systems Engineering: Conference Presentations and White Papers

Transfected cell arrays represent a high-throughput approach to correlate gene expression with functional cell responses, based on gene delivery from a substrate that supports cell adhesion. These arrays provide the ability to express, in parallel, thousands of exogenous genes in live cells, giving real-time information on cellular physiology and gene function. While there have been advances in transfected cell arrays, improvements are needed to this technology, including increasing the cells types that can be efficiently transfected and developing better quantification and normalization methods. We have created an array using soft lithography techniques to pattern DNA-lipid complex deposition. Specifically, a mold …


Controlled Surface-Associated Delivery Of Genes And Oligonucleotides, Tatiana Segura, Lonnie D. Shea, Angela K. Pannier, Zain Bengali, Jae-Hyung Jang, Peter Chung, Brian C. Anderson Apr 2006

Controlled Surface-Associated Delivery Of Genes And Oligonucleotides, Tatiana Segura, Lonnie D. Shea, Angela K. Pannier, Zain Bengali, Jae-Hyung Jang, Peter Chung, Brian C. Anderson

Biological Systems Engineering: Papers and Publications

A system and methods for controlled gene delivery comprising condensed nucleic acids complexed with polylinkers, wherein the complexes are covalently and/or noncovalently bound to the surface of a substrate capable of supporting cell adhesion. The gene delivery system achieves temporal and spatial control of nucleic acid delivery to a target cell or cells through control of complex density on the surface of the support substrate, and reversibility of the attachment of the polylinker to the support substrate. The system and method of the invention can be used to create spatial patterns of gene expression, and in tissue engineering, high throughput …


Updated Asabe Standard Manure Excretion Standard, Richard K. Koelsch Apr 2006

Updated Asabe Standard Manure Excretion Standard, Richard K. Koelsch

Department of Biological Systems Engineering: Conference Presentations and White Papers

This newsletter introduces the new ASABE (American Society of Agricultural and Biological Engineers) Standard D384.2, Manure Production and Characteristics. This new standard provides an equation-based approach that integrates animal feeding program and performance into the final estimate of total solids, nitrogen, and phosphorus excretion for seven livestock and poultry species. This article reviews the opportunity this standard provides for improving accuracy of farm-specific nutrient management planning and introduces an educational opportunity for users to be introduced to new software for using the standard.

I propose to conduct one or more workshops on the standard and supporting software tool in June. …


Vegetative Treatment Systems For Management Of Open Lot Runoff: Review Of Literature, Richard K. Koelsch, Jeffrey C. Lorimer, Kyle R. Mankin Jan 2006

Vegetative Treatment Systems For Management Of Open Lot Runoff: Review Of Literature, Richard K. Koelsch, Jeffrey C. Lorimer, Kyle R. Mankin

Biological Systems Engineering: Papers and Publications

Runoff from open lot livestock systems (beef and dairy) defined as Concentrated Animal Feeding Operations (CAFO) must be controlled by systems designed and managed to prevent the release of manure-contaminated runoff for storms equal to or less than a 25-yr, 24-h design storm. This performance standard has been attained for open lot systems with some combination of clean water diversion, settling basins, runoff collection ponds, and irrigation systems (baseline system).

An alternative approach is to rely on overland flow and infiltration into cropland with perennial forage or grasses for treatment of open lot runoff. Such vegetative systems have been researched …


Quantitative Measurement Of The Brã¶Nsted Acid Sites In Solid Acids: Toward A Single-Site Design Of Mo-Modified Zsm-5 Zeolite, Jean-Philippe Tessonnier, Benoit Louis, Stephane Walspurger, Jean Sommer, Marc-Jacques Ledoux, Cuong Pham-Huu Jan 2006

Quantitative Measurement Of The Brã¶Nsted Acid Sites In Solid Acids: Toward A Single-Site Design Of Mo-Modified Zsm-5 Zeolite, Jean-Philippe Tessonnier, Benoit Louis, Stephane Walspurger, Jean Sommer, Marc-Jacques Ledoux, Cuong Pham-Huu

Jean-Philippe Tessonnier

On the basis of our previous H/D exchange studies devoted to the quantification of the number of Bro¨nsted acid sites in solid acids, we report here an innovative approach to determine both the amount and the localization of Mo atoms inside the Mo/ZSM-5 catalyst, commonly used for the methane dehydroaromatization reaction. The influence of Mo introduction in the MFI framework was studied by means of BET, X-ray diffraction, 27Al magic angle spinning NMR, NH3 temperature-programmed desorption, and H/D isotopic exchange techniques. A dependence was found between the decrease of acidic OH groups and the Mo content. Depending on the Si/Al …


Intracellular Signaling Networks In The Immune Response: Pathways Activated By Interleukin-2 And-4 Receptors And Their Roles In T Cell Proliferation, Kristen K. Comfort Jan 2006

Intracellular Signaling Networks In The Immune Response: Pathways Activated By Interleukin-2 And-4 Receptors And Their Roles In T Cell Proliferation, Kristen K. Comfort

Chemical and Materials Engineering Faculty Publications

Cells sense and respond to chemical and physical stimuli through signal transduction pathways, which mediate cell proliferation, differentiation, migration, and survival. The cytokines interleukin-2 (IL-2) and interleukin-4 (IL-4) are key regulators of the adaptive immune system, particularly influencing the clonal expansion and differentiation of T cells. At least in culture, both synergistic and antagonistic effects of IL-2 and -4 co-stimulation have been reported; the antagonism, when observed, is thought to arise from the utilization of a common subunit shared by IL-2 and IL-4 receptors. We have sought to characterize IL-2 and IL-4 signaling at the level of intracellular pathways activated …


Postharvest Treatment To Control Codling Moth In Fresh Apples Using Water Assisted Radio Frequency Heating, Shaojin Wang, Sohan Birla, J. D. Hansen Jan 2006

Postharvest Treatment To Control Codling Moth In Fresh Apples Using Water Assisted Radio Frequency Heating, Shaojin Wang, Sohan Birla, J. D. Hansen

Biological Systems Engineering: Papers and Publications

Apples destined for export to Japan and South Korea are currently disinfested for codling moth, Cydia pomonella L. (Lepidoptera: Tortricidae), using methyl bromide fumigation. Restrictions and limitations imposed on the uses of methyl bromide have increased interest in developing alternative non-chemical quarantine treatments. It is imperative that the treatment is effective against codling moth yet maintains the quality of treated apples. The present study explored the application of radio frequency (RF) energy in conjunction with conventional hot water treatment to develop feasible heat treatments. Treatment parameters were selected based on minimum time–temperature conditions required for 100% mortality of fifth-instar codling …


A Mathematical Model For The Validation Of Safe Air-Blast Chilling Of Cooked Hams, L. J. Wang, A Amézquita, Curtis L. Weller Jan 2006

A Mathematical Model For The Validation Of Safe Air-Blast Chilling Of Cooked Hams, L. J. Wang, A Amézquita, Curtis L. Weller

Biological Systems Engineering: Papers and Publications

An integrated mathematical model of heat transfer and temperature-dependent bacterial growth was developed to validate the safety of cooked hams during air-blast chilling. Heat transfer through a cooked ham was mathematically modeled and analyzed with a finite element method. Response of bacteria to temperatures was quantitatively described using predictive microbiology. The cumulative effect of temperature history on the bacterial growth was taken into account in the model. For chilling cooked hams from 71°C to 10°C, the maximum error between the predicted and experimental core temperature was within 2.2°C, and the deviation between the predicted and measured total weight losses was …


Modeling Of Transport Phenomena And Melting Kinetics Of Starch In A Co-Rotating Twin-Screw Extruder, Lijun Wang, David D. Jones, Curtis L. Weller, Milford Hanna Jan 2006

Modeling Of Transport Phenomena And Melting Kinetics Of Starch In A Co-Rotating Twin-Screw Extruder, Lijun Wang, David D. Jones, Curtis L. Weller, Milford Hanna

Biological Systems Engineering: Papers and Publications

A mathematical model was developed to simulate fluid flow, heat transfer, and melting kinetics of starch in a co-rotating intermeshing twin-screw extruder (TSE). The partial differential equations governing the transport phenomena of the biomaterial in the extruder were solved by a finite element scheme. For validating the model, the predicted product pressure, bulk temperature at the entrance of the die, and minimum residence time of the biomaterial in the extruder were compared with experimental data. Standard errors of product pressure, bulk temperature at the die entrance, and minimum residence time were about 8.8, 2.8, and 17.3%. Simulations were carried out …


Impact Of Tillage And Placement Of Terbufos Insecticide Runoff, Mitiku Mamo, William L. Kranz, Elaine R. Douskey, Shripat T. Kamble, John F. Witkowski Jan 2006

Impact Of Tillage And Placement Of Terbufos Insecticide Runoff, Mitiku Mamo, William L. Kranz, Elaine R. Douskey, Shripat T. Kamble, John F. Witkowski

Biological Systems Engineering: Papers and Publications

On a national scale, the damage caused by corn rootworm (Diabrotica virgifera virgifera LeConte), can cause economic loss of up to $650 million annually. To limit these losses, corn producers apply terbufos insecticide to approximately 11% of all corn acres at a time when there is a high probability of intense precipitation. When combined with low vegetative cover and disturbed soil surfaces, there is a high potential for terbufos transport into surface water bodies. Increased public concern for environmental contamination from the use of agricultural chemicals has prompted many crop growers to look for crop production practices that minimize …


Use Of Time Domain Reflectometry For Continuous Monitoring Of Nitrate-Nitrogen In Soil And Water, J. O. Payero, David D. Tarkalson, Suat Irmak Jan 2006

Use Of Time Domain Reflectometry For Continuous Monitoring Of Nitrate-Nitrogen In Soil And Water, J. O. Payero, David D. Tarkalson, Suat Irmak

Biological Systems Engineering: Papers and Publications

Nitrate-Nitrogen (NO3-N) losses to ground and surface water are an environmental and agronomic concern in modern crop production systems in the Central Great Plains. Monitoring techniques for nitrogen use in agricultural production are needed to increase crop yield, optimize nitrogen use, and reduce NO3-N leaching. Time domain reflectometry (TDR) could potentially be calibrated to continuously measure NO3-N in soil and water. The objectives of this study were to: (1) evaluate the effect of different factors affecting the response of the bulk electrical conductivity (ECb) sensed by TDR, (2) compare the sensitivity and differences between …


Variable Upper And Lower Crop Water Stress Index Baselines For Corn And Soybean, J. O. Payero, Suat Irmak Jan 2006

Variable Upper And Lower Crop Water Stress Index Baselines For Corn And Soybean, J. O. Payero, Suat Irmak

Biological Systems Engineering: Papers and Publications

Upper and lower crop water stress index (CWSI) baselines adaptable to different environments and times of day are needed to facilitate irrigation scheduling with infrared thermometers. The objective of this study was to develop dynamic upper and lower CWSI baselines for corn and soybean. Ten-minute averages of canopy temperatures from corn and soybean plots at four levels of soil water depletion were measured at North Platte, Nebraska, during the 2004 growing season. Other variables such as solar radiation (Rs), air temperature (Ta), relative humidity (RH), wind speed (u), and plant canopy height (h) were …


Circular Ultrasound Compounding By Designed Matrix Weighting, Gregory Bashford, Jonathan Morse Jan 2006

Circular Ultrasound Compounding By Designed Matrix Weighting, Gregory Bashford, Jonathan Morse

Biological Systems Engineering: Papers and Publications

Spatial compounding is an imaging technique that aims to improve image contrast by combining partially decorrelated images acquired at different angles or positions. In conventional spatial compounding, data sets are combined with equal weighting. Here, we describe an alternative method of reconstruction using algorithms which weight the data based on a “quality” matrix. The quality matrix is derived from beam-forming characteristics. For each data set, the reliability of the data is assumed to vary spatially. By compounding the data based on the quality matrix, a complete image is formed. Here, we describe the construction of a rotational translation stage and …


Land Application Of Manure For Beneficial Reuse, L. M. Risse, M. L. Cabrera, A. J. Franzluebbers, J. W. Gaskin, John E. Gilley, R. Killorn, D. E. Radcliffe, W. E. Tollner, H. Zhang Jan 2006

Land Application Of Manure For Beneficial Reuse, L. M. Risse, M. L. Cabrera, A. J. Franzluebbers, J. W. Gaskin, John E. Gilley, R. Killorn, D. E. Radcliffe, W. E. Tollner, H. Zhang

Biological Systems Engineering: Papers and Publications

The concentration of animal production systems has increased efficiency and improved overall economic return for animal producers. This concentration, along with the advent of commercial fertilizers, has led to a change in the way animal producers view manure. Manure, once valued as a resource by farmers, is now treated as a waste. Air and water quality concerns that arise primarily from the under-utilization or inefficient use of manure contribute to these changing views. However, when properly used, manure is a resource and should be regulated as such. In the United States, the USDA/EPA Unified National Strategy for Animal Feeding Operations …


Properties Of Chitosan Films As A Function Of Ph And Solvent Type, Ki Myong Kim, Jeong Hwa Son, Sung-Koo Kim, Curtis L. Weller, Milford Hanna Jan 2006

Properties Of Chitosan Films As A Function Of Ph And Solvent Type, Ki Myong Kim, Jeong Hwa Son, Sung-Koo Kim, Curtis L. Weller, Milford Hanna

Biological Systems Engineering: Papers and Publications

Two different deacetylated chitosans were dissolved in formic, acetic, lactic, or propionic acid to prepare chitosan films. The pH values of the film-forming solutions were adjusted to 3, 4, and 5. Water vapor permeability (WVP), tensile strength (TS), elongation (E), and total soluble matter (TSM) were significantly (P < 0.05) affected by acid type, pH, and degree of deacetylation (DA). Low DA (LDA) chitosan films had lower WVP and TSM, higher TS compared with high DA (HDA) chitosan films. The E values were not affected by DA. As pH increased, WVP and TSM of chitosan films tended to increase while TS decreased significantly (P < 0.05). Chitosan films with acetic and propionic acid solvents had low WVP and TSM and high TS, while films with lactic acid solvent had high E and TSM and the lowest TS. Fourier-transform infrared showed peak shifting in the spectra with different solvents and at different pH values. Chitosan films with lactic acid solvent showed a peak shift to a lower frequency range. The NH3+ band was absent in the pH 5.0 chitosan film spectra.