Biomedical Engineering and Bioengineering Commons^™

Optimizing Channel Formation In Peg Maleimide Hydrogels, Bakthavachalam Kannadasan

Masters Theses

Blood vessels including the arteries, veins, and capillaries are a critical and indispensable component of various organisms. Some studies estimate that if all the blood vessels present in our body are arranged in line, they would amount to a total length of approximately 60,000 miles. This distance is enough to circle the world two and a half times! In addition to being all pervasive, blood vessels perform certain key functions such as delivery of oxygen and nutrients to various tissues in the body. They also assist in the spread of diseases such as cancer. Therefore, it is important to study …

Enhancing Human Key Point Identification: A Comparative Study Of High-Resolution Vicon Dataset And Coco Dataset Using Bpnet, Bibash Lama

Masters Theses

Accurately identifying human key points is crucial for various applications, including activity recognition, pose estimation, and gait analysis. This study presents a high-resolution dataset created using the VICON motion capture system and three differently oriented 2D cameras, that can be used to train different neural networks for estimating the 2D key joint positions of the person from the 2D images or videos. The participants in the study included 25 healthy adults (17 males and 8 females) performing normal gait movements for about 2 to 3 seconds. The VICON system captured 3D ground truth data, while the three 2D cameras collected …

Fungi In Flux | Designing Regenerative Materials And Products With Mycelium, Arvind Bhallamudi

Masters Theses

As the world grapples with the escalating crisis of climate threats and environmental degradation, this research delves into the synergistic potential of design and biology, developing safe and sustainable materials for applications in prototyping, furniture and interior design. Harnessing the power of a unique organism - fungi, the study proposes an accessible, efficient, and resilient material resource system. It utilizes local waste streams and mycelium (the vegetative part of fungi) to grow functional structures. An experimental and small-scale protocol is modeled by testing bio-fabrication and bio-printing methods. The composites' performance qualities and characteristics are evaluated through mechanical testing and a …

Unveiling Pain: Wearables For Objective Pain Measurement, Hanqing Tang

Masters Theses

">">Pain perception is a subjective experience that differs significantly among individuals, often leading to inconsistencies in assessment and management. A critical issue within this context is the gender bias in pain evaluation, which contributes to unequal treatment and perpetuates gender inequality within the healthcare system. This thesis presents an in-depth investigation of the problem and proposes the development of a wearable device for objective pain assessment. Physiological parameters — Electrocardiography (ECG) can be collected from cardiac sound signals auscultated by fabrics via nanometre-scale vibrations. Machine learning methods can accurately classify heart rate and acute pain intensity of participants. …

Effect Of Amputation On Muscle Structure Properties In A Rabbit Model, Roy Caleb Stubbs

Masters Theses

After amputation, muscles in the residual limb are detached from their insertion points and no longer span the missing joints. Our objective was to quantify the effect of amputation-induced disuse on residual muscle structure, an indirect indicator of muscle force-generating capacity. One hind paw was surgically removed at the ankle joint of ten rabbits. At two weeks (n=5) and 4 weeks (n=5) post-amputation and for select muscles (gastrocnemius, soleus, tibialis cranialis, extensor digitorum, and flexor digitorum superficialis), we measured and computed several muscle structure properties. Additionally, we qualitatively assessed the muscle fiber appearance of histological samples at each timepoint. At …

Iron Nanoparticles For Magnetic Imaging Applications, Aleia Williams

Masters Theses

Extensive research on iron oxide nanoparticles for various applications including nanomedicine, energy applications, environmental remediation, and magnetic imaging have previously been performed. Many are currently FDA approved as magnetic resonance imaging contrast agents and tracers for magnetic particle imaging applications. Magnetic properties of such materials are crucial to obtain good contrast and resolution. However, studies have shown the magnetic properties of iron oxide nanoparticles are less in comparison to those found in pure iron nanoparticle.

This research involves the synthesis and characterization of iron nanoparticles for applications in magnetic resonance imaging contrast agents, magnetic particle imaging tracers, and therapeutic agents …

Optimized 3d-Printing Of Carbon Fiber-Reinforced Polyether-Ether-Ketone (Cfr-Peek) For Use In Overmolded Lattice Composite, Ryan C. Ogle

Masters Theses

Current orthopedic implants are overwhelmingly composed from metallic materials. These implants show superior mechanical properties, but this can additionally result in stress shielding due to a modulus mismatch between the bone tissue and implanted device. Polymeric implants reduce this stress shielding effect but have much lower mechanical properties, limiting their use. Polylactic acid (PLA) is a widely used biodegradable thermoplastic polymer, however, its use has been limited by the polymer’s mechanical properties and rapid loss of strength during degradation in vivo. Polyether-ether-ketone (PEEK) is another common biocompatible polymer , with chemical and mechanical properties which make it a popular alternative …

Encapsulation Of Antimicrobial Peptides In Bicontinuous Microemulsions For Topical Delivery To Surgical Site Infections And Chronic Wounds, Madison A. Oehler

Masters Theses

Surgical site infections and chronic wounds, especially those caused by antibiotic-resistant microorganisms, result in hospitalization and fatalities each year. Methods to prevent these infections, such as cleaning and preparing medical tools, have had minimal success in preventing infections. Further, antibiotic treatments have become less successful in treating infections and wounds because of antibiotic-resistant bacteria. Antimicrobial peptides (AMP) are a possible treatment solution for wound infections. AMPs are oligopeptides that occur in nature or can be synthesized in vitro which possess a broad spectrum of antimicrobial activity against bacteria and other harmful microorganisms. AMPs operate by disrupting the packing arrangements of …

Controlled Codelivery Of Mir-26a And Antagomir-133a With Osteoconductive Scaffolds To Promote Healing Of Large Bone Defects, Cole J. Ferreira

Masters Theses

Often caused by trauma or tumor removal, large bone defects frequently result in delayed or non-union. The current gold standard for treatment is autograft. However, due to limitations, such as the size and location of the defect, these cannot always be utilized. A common alternative to autograft is the use of BMP-2 with a collagen scaffold, however, this treatment is limited by numerous side effects. In recent years, genetic materials such as microRNAs (miRNAs) have offered possible alternative therapies. MiRNAs are small non-coding RNA molecules that generally range from 20-24 nucleotides, serve as repressors of gene expression, and are involved …

Increasing Hydrophilicity And Transparency Of Diamond-Like Carbon Thin Films With Dopants For An Anti-Fogging Laparoscope Coating, Anna Bull

Masters Theses

Laparoscopes are prone to fogging which can lead to a limited field of view during surgical procedures. Current methods of mitigating fogging issues are not efficient or can require costly modification to the laparoscope. Previous studies of diamond-like carbon (DLC) coatings found doping the films improved hydrophilic qualities, suggesting their possible use as antifogging coatings for laparoscopes. For this work, two series of DLC films, doped with either SiO and Al₂O₃ were investigated.

The biocompatibility, transparency, and stability of these films were assessed through cellular assays, spectrophotometry, and simulated body fluid soaking experiments. Contact angle and surface …

Visuomotor Adaptation During Asymmetric Walking, Charles Napoli

Masters Theses

Necessary for effective ambulation, head stability affords optimal conditions for the perception of visual information during dynamic tasks. This maintenance of head-in-space equilibrium is achieved, in part, by the attenuation of the high frequency impact shock resulting from ground contact. While a great deal of experimentation has been done on the matter during steady state locomotion, little is known about how head stability or dynamic visual acuity is maintained during asymmetric walking.

In this study, fifteen participants were instructed to walk on a split-belt treadmill for ten minutes while verbally reporting the orientation of a randomized Landolt-C optotype that was …

Cfd Analysis Of Subcutaneous Deposition And Dispersion Of Insulin In Adipose Tissue, Ryan C. Lubbers

Masters Theses

Drug delivery is the most important factor of many therapies, but a lack of technology and research have led to a very generalized understanding of drug kinetics. Insulin pump therapy for the treatment of Type 1 Diabetes depends on precise delivery of the hormone into the adipose region between the dermis and underlying muscle. The kinetics of insulin within the adipose tissue environment is not well understood and varies greatly case to case. The use of computation fluid dynamics (CFD) models to study insulin kinetics in relation to influential factors will lead to a better understanding of the characteristics of …

Rare Earth-Doped Glass-Ceramic Scintillators As X-Ray Flat Panel Detector Substrates, Austin M. Thomas

Masters Theses

Digital radiography (DR) is an important two-dimensional imaging technique in the field of medicine that utilizes x-rays to form a digital image. DR employs a flat panel detector that converts incident x-rays, that have passed through the subject, to an electrical signal, which is used to create a digital image. The conversion from x-rays to electrical signals can be done either directly or indirectly. The direct method involves the x-rays being converted to an electrical signal via an array of semiconductors. The indirect method utilizes scintillators to absorb the x-rays and produce light in the visible spectrum, which is then …

In Vitro And In Vivo Response To A Novel Borophosphate Bioactive Glass, Nada Abokefa

Masters Theses

"Bioactive glasses have been widely used in several biomedical and tissue engineering applications since the late 1960s. Numerous families of bioactive glasses have emerged over the years, with each having its own advantage and disadvantage. One concern common to all families is that most bioactive glasses create a basic or acidic environment when degraded and, therefore, are toxic for cells. Recently, there has been an increased interest in borophosphate bioactive glass (BPBGs) because of their neutral pH, release of therapeutic ions, and biodegradable properties. Despite the growing interest, there is little reported on the bioactivity of BPBGs. The biological effects …

An Investigation Into The Development Of A Low Cost, Easy To Use Seizure Analysis Tool., Cody Dean

Masters Theses

The need for collaborating and sharing data and research between doctors, researchers, universities and patients has never been more necessary. We are seeing firsthand how a deadly virus can completely devastate the world in a matter of months and being able to react quickly is the top priority. Open source tools are making it possible to share research and learnings about viruses like COVID-19 across countries, industries, and universities and these tools and philosophies extend across all areas of medical research.

The amount of data that is being collected within the medical industry is increasing at an exponential rate and …

Cellular Attachment On Poly(Lactic Acid) For Use In Tissue Engineering, Stephen R. Hunter

Masters Theses

Tissue engineering is a promising new method for organ regeneration. This method can be used to repair damaged tissue, or possibly replace a fully functional organ. In summary, a biopsy is taken from a donor, isolated, then grown onto a framework resembling an organ. Once grown; it is then transplanted into the patient. Over time, the body will degrade the scaffold, leaving the organ or tissue in its place. As this scaffold is present in the body for an extended time; factors like biocompatibility, toxicity, immunogenicity, and structural stability must be researched.

Several polymers have already been researched for use …

Assessment Of The Use Of Low Molecular Weight Diblock Copolymers For The Formation Of Stable, Tunable Droplet Interface Bilayers, Joseph Tawfik

Masters Theses

This thesis presents the use of diblock copolymers, poly(butadiene)-b-poly(ethylene oxide) (PB_m PEO_n) and poly(isoprene)-b-poly(ethylene oxide) (PI_mPEO_n), as amphiphilic molecular building blocks for the formation of synthetic polymer bilayer membranes using the droplet interface bilayer (DIB) technique. The DIB technique makes use of the self-assembly of amphiphilic macromolecules along oil-water droplet interfaces that can then be physically connected for the construction of liquid supported macromolecular bilayers at the droplet interface. These bilayer membranes are capable of hosting both naturally occurring and synthetic protein channels. This technique has been used to form synthetic bilayer membranes …

Camera-Based Remote Photoplethysmography For Estimation Of Heart Rate Using Single Board Computers, Benjamin Sweely

Masters Theses

The objective of this project was to develop a wireless, noncontact monitoring system that measures multiple physiological parameters in human faces from a distance using a camera. Compared to traditional sensors, this monitoring system does not use wires or adhesives, providing a safer, more user-friendly application. The goal of the monitoring systems was to estimate heart rate (HR). The current practices of measuring HR involve collecting electrocardiogram (ECG) signals from adhesive electrodes placed on various parts of the body and using a pulse oximeter (PO) typically placed on the ear lobe or finger. We were able to successfully create 2 …

Changes In Neural Network Connectivity Of Normal Young Adults In Response To Power Wheelchair Trainer Using Eeg Coherence, Ruby Phung

Masters Theses

The ability to move and explore their surrounding environment plays a critical role in the development of cognitive function in children, especially during early childhood. The lack of independent and autonomous mobility is, therefore, a clear disadvantage for the overall development of children with multiple, severe disabilities. Limited number of studies have been conducted on the impact of power mobility device on this specific population. Previous exploratory and pilot studies showed promising results regarding quantifiable and consistent changes in the electroencephalogram (EEG) of children with multiple, severe disabilities when provided with power mobility training. This study aimed to further extend …

Analysis Of Center Of Pressure And Center Of Mass To Determine Patterns Of Postural Stability In Neurotypical Subjects, Natalie Tipton

Masters Theses

The body’s postural control mechanism is responsible for responding to perturbations of balance and keeping the body upright. One of the main ways that this is completed during quiet standing, where both feet are planted on the ground, is through center of pressure oscillations. In these oscillations, the center of pressure circles around the center of mass, constantly counteracting any lean that exists in the body. These oscillations can be recorded with floorembedded force plates and center of mass can be recorded with a motion capture system. In this research, these signals were recorded for stances with feet together and …

The Design And Development Of A Device To Assist In Boosting Patients, Taylor A. Rieckhoff

Masters Theses

A common task a nurse is required to perform is called boosting patients. Boosting a patient is defined as lifting or sliding a patient back up in the bed after having slid down (Mannheim, Zieve, & Conaway, 2017). The current method for boosting patients involves a minimum of two personnel and an 11-step process. The 11-step process requires the person to manually lift and pull the patient using an existing half sheet on the bed (Mannheim, Zieve, & Conaway, 2017). Patients who cannot move or support themselves are moved every two to six hours or upon request (Bihn, Rieckhoff, Burkman, …

Differentiating Epileptic From Psychogenic Nonepileptic Eeg Signals Using Time Frequency And Information Theoretic Measures Of Connectivity, Sarah Barnes

Masters Theses

Differentiating psychogenic nonepileptic seizures from epileptic seizures is a difficult task that requires timely recording of psychogenic events using video electroencephalography (EEG). Interpretation of video EEG to distinguish epileptic features from signal artifacts is error prone and can lead to misdiagnosis of psychogenic seizures as epileptic seizures resulting in undue stress and ineffective treatment with antiepileptic drugs. In this study, an automated surface EEG analysis was implemented to investigate differences between patients classified as having psychogenic or epileptic seizures. Surface EEG signals were grouped corresponding to the anatomical lobes of the brain (frontal, parietal, temporal, and occipital) and central coronal …

Methods Of Estimating Foot And Ankle Power In Takeoff Phase Of Standing Vertical Jump, Kundan Joshi

Masters Theses

Experimental motion capture studies have commonly considered the foot as a single rigid body. However, the presence of internal joints as well as soft-tissue interactions inside the foot demonstrate that the foot is in fact not rigid. Various methods have been applied to study the deviations of the foot from rigid body mechanics, such as developing multi-segment foot models or employing compensation strategies for the rigid foot model. However, no study has compared these compensation strategies with multi-segment models in any movement such as gait or jumping. This study compared two main compensation strategies (distal foot power and power balance …

The Transcriptional Response Of Phanerochaete Chrysosporium And Trametes Versicolor To Growth On Stems Of Helianthus Argophyllus (Silverleaf Sunflower), Nadh Hamoud Alsubaie

Masters Theses

Traditional physicochemical and physicomechanical pretreatment technologies have improved the deconstruction of lignocellulose, but often require high energy inputs and added chemical reagents. Recent studies have demonstrated the use of white-rot fungi as alternative biological pretreatment agents that can mitigate these concerns. Although, white-rot fungi have been shown to grow on a variety of non-woody substrates, they have not been explored with respect to sunflower, which has the potential to provide raw materials for both biodiesel and bioethanol. In the present study, Silverleaf sunflower stems (Helianthus argophyllus) were used as substrates for the white-rot fungi Phanerochaete chrysosporium and Trametes …

Time Evolution Of Ecog Network Connectivity In Patients With Refractory Epilepsy, Michael J. Selesko

Masters Theses

Epilepsy is a common neurological disorder that causes recurrent, unprovoked seizures. Focal, refractory epilepsy is characterized by seizures that start in a specific area in the brain and do not respond to antiepileptic medication. This study was conducted to characterize the connectivity of the focal brain region where seizures originate, known as the epileptogenic zone. Connectivity was measured from electrocorticogram (ECoG) recordings, during seizure events, using information theoretic measures mutual information (MI) and (TE). MI is the amount of information shared between two discrete random variables. TE is the directed information transfer between two discrete random variables. MI provides information …

Identification Of The Seizure Onset Zone By Auto-Regressive Model Residual Modulation Applied To Intracranial Eeg And Its Correlation To Channels With High Preponderances Of Detected Hfos, Allison L. Rogutich

Masters Theses

The objective of this thesis was to examine the ability of the Autoregressive Model Residual Modulation (ARRm) method to identify the Seizure Onset Zone (SOZ) in intracranial electroencephalogram (iEEG) of patients with refractory epilepsy. Patients who have not become seizure free after multiple trials of antiepileptic drugs (AEDs) may seek treatment through epilepsy surgery. Cortical electrodes are implanted directly on the cerebral cortex, then iEEG is collected. A specialized neurologist reviews the iEEG, then in consultation with the neurosurgeon, the SOZ is determined and areas of the brain may be chosen for resection. The success rate of epilepsy surgery varies, …

Effects Of Malformed Or Absent Valves To Lymphatic Fluid Transport And Lymphedema In Vivo In Mice, Akshay S. Pujari

Masters Theses

Lymph is primarily composed of fluid and proteins from the blood circulatory system that drain into the space surrounding cells, interstitial space. From the interstitial space, the fluid enters and circulates in the lymphatic system until it is delivered into the venous system. In contrast to the blood circulatory system, the lymphatic system lacks a central pumping organ dictating the predominant driving pressure and velocity of lymph. Transport of lymph via capillaries, pre-collecting and collecting lymphatic vessels relies on the synergy between pressure gradients, local tissue motion, valves and lymphatic vessel contractility. The direction of lymph transport is regulated by …

Design Of A Robotic Instrument Manipulator For Endoscopic Deployment, Ryan Ponten

Masters Theses

This thesis describes the initial design process for an application of continuum robotics to endoscopic surgical procedures, specifically dissection of the colon. We first introduce the long-term vision for a benchtop dual-instrument endoscopic system with intuitive haptic controllers and then narrow our focus to the design and testing of the instrument manipulator itself, which must be actuated through the long, winding channel of a standard colonoscope.

Based on design requirements for a target procedure, we analyze simulations of two types of continuum robots using recently established kinematic and mechanic modeling approaches: the concentric-tube robot (CTR) and the concentric agonist-antagonist robot …

A Magnetic Resonance Compatible Knee Extension Ergometer, Youssef Jaber

Masters Theses

The product of this thesis aims to enable the study of the biochemical and physical dynamics of the lower limbs at high levels of muscle tension and fast contraction speeds. This is accomplished in part by a magnetic resonance (MR) compatible ergometer designed to apply a load as a torque of up to 420 Nm acting against knee extension at speeds as high as 4.7 rad/s. The system can also be adapted to apply the load as a force of up to 1200 N acting against full leg extension. The ergometer is designed to enable the use of magnetic resonance …

3d Bioprinting A Pcl/13-93b3 Glass Composite And Its Potential Use As A Bio-Ink, Caroline Blair Murphy

Masters Theses

"A major limitation of using synthetic scaffolds in tissue engineering is little growth of incorporated cells in the interior of the scaffold, resulting in insufficient angiogenesis in the scaffold interior. Recently, cells have been 3D bioprinted concurrently with biomaterials to produce a cellularized, bioactive, angiogenic 3D environment. This thesis describes a novel solvent-extrusion method for printing polycaprolactone (PCL)/bioactive borate glass composite as a biomaterial for a cell-laden scaffold.

Bioactive borate glass was added to a mixture of PCL and organic solvent to make an extrudable paste, creating scaffolds measuring 10×10×1 mm³ in overall dimensions with pore sizes ranging from …