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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
Applied Machine Learning In Extrusion-Based Bioprinting, Shuyu Tian
Applied Machine Learning In Extrusion-Based Bioprinting, Shuyu Tian
Theses and Dissertations
Optimization of extrusion-based bioprinting (EBB) parameters have been systematically conducted through experimentation. However, the process is time and resource-intensive and not easily translatable across different laboratories. A machine learning (ML) approach to EBB parameter optimization can accelerate this process for laboratories across the field through training using data collected from published literature. In this work, regression-based and classification-based ML models were investigated for their abilities to predict printing outcomes of cell viability and filament diameter for cell-containing alginate and gelatin composite hydrogels. Regression-based models were investigated for their ability to predict suitable extrusion pressure given desired cell viability when keeping …
An Injectable Stem Cell Delivery System For Treatment Of Musculoskeletal Defects, Shirae Leslie
An Injectable Stem Cell Delivery System For Treatment Of Musculoskeletal Defects, Shirae Leslie
Theses and Dissertations
The goal of this research was to develop a system of injectable hydrogels to deliver stem cells to musculoskeletal defects, thereby allowing cells to remain at the treatment site and secrete soluble factors that will facilitate tissue regeneration. First, production parameters for encapsulating cells in microbeads were determined. This involved investigating the effects of osmolytes on alginate microbead properties, and the effects of alginate microbead cell density, alginate microbead density, and effects of osteogenic media on microencapsulated cells. Although cells remained viable in the microbeads, alginate does not readily degrade in vivo for six months. Therefore, a method to incorporate …
Tissue Engineering An Acellular Bioresorbable Vascular Graft To Promote Regeneration, Patricia Wolfe
Tissue Engineering An Acellular Bioresorbable Vascular Graft To Promote Regeneration, Patricia Wolfe
Theses and Dissertations
Tissue engineering is an interdisciplinary field that aims to restore, maintain, or improve diseased or damaged tissues. Electrospinning has become one of the most popular means to fabricate a scaffold for various tissue engineering applications as the process is extremely versatile and inexpensive. The ability for electrospinning to consistently create nanofibrous structures capable of mimicking the native extracellular matrix (ECM) is the basis behind why this technique is so successful in tissue engineering. Cardiovascular disease has been the leading cause of death in the United States for over 100 years, and because of this, the need for coronary artery replacements …
Development Of Physiologic Contact Models For Articular Surfaces, John Owen
Development Of Physiologic Contact Models For Articular Surfaces, John Owen
Theses and Dissertations
The superficial tangential zone (STZ) plays a significant role in normal articular cartilage’s ability to support loads and retain fluids. To date, tissue engineering efforts have not replicated normal STZ function in cartilage repairs. Finite element models were developed to examine the STZ’s role in normal and repaired articular surfaces under different contact conditions. Models were developed by incrementally adding improvements which culminated in contact loading of curved models by permeable and impermeable rigid surfaces and a normal cartilage layer. In the normal STZ, permeability was strain-dependent on volumetric strain; tension-compression nonlinearity modeled collagen behavior. Nonlinear geometry accounted for finite …
Effect Of Mechanical Stimulation On Mesenchymal Stem Cell Seeded Cartilage Constructs, Karin Wartella
Effect Of Mechanical Stimulation On Mesenchymal Stem Cell Seeded Cartilage Constructs, Karin Wartella
Theses and Dissertations
Cartilage tissue engineered constructs using mesenchymal stem cells were stimulated with 3 different stimulation algorithms to achieve characteristics mimicking the superficial tangential zone of articular cartilage. The stimulation algorithm of both compression and tension without an offset had the best properties out of all the evaluated groups.
Design Of An Electrospun Type Ii Collagen Scaffold For Articular Cartilage Tissue Engineering, Catherine Pemble Barnes
Design Of An Electrospun Type Ii Collagen Scaffold For Articular Cartilage Tissue Engineering, Catherine Pemble Barnes
Theses and Dissertations
Traumatic defects in articular cartilage can lead to joint disease and disability including osteoarthritis. Because cartilage is unable to regenerate when injured, the field of tissue engineering holds promise in restoring functional tissue. In this research, type II collagen was electrospun, cross-linked, and tested as scaffolds for supporting chondrocyte growth. The mechanical, biochemical, and histological characteristics of the engineered tissue were assessed as a function of the electrospinning solution concentration (i.e. scaffold fiber diameter and pore properties) and as a function of the time in culture (evaluated at 2, 4, and 6 weeks). Fiber diameter had a linear relationship with …
Scaffold Permeability As A Means To Determine Fiber Diameter And Pore Size Of Electrospun Fibrinogen, Scott Allen Sell
Scaffold Permeability As A Means To Determine Fiber Diameter And Pore Size Of Electrospun Fibrinogen, Scott Allen Sell
Theses and Dissertations
The purpose of this study was to construct a flowmeter that could accurately measure the hydraulic permeability of electrospun fibrinogen scaffolds, providing insight into the transport properties of electrospun scaffolds while making the measurement of their topographical features (fiber and pore size) more accurate. Three different concentrations of fibrinogen were used (100, 120, and 150mg/ml) to create scaffolds with three different fiber diameters and pore sizes. The fiber diameters and pore sizes of the electrospun scaffolds were analyzed through scanning electron microscopy and image analysis software. The permeability of each scaffold was measured and used to calculate permeability-based fiber diameters …
Determination Of The Mechanical Properties Of Electrospun Gelatin Based On Polymer Concentration And Fiber Alignment, Leander Taylor Iii
Determination Of The Mechanical Properties Of Electrospun Gelatin Based On Polymer Concentration And Fiber Alignment, Leander Taylor Iii
Theses and Dissertations
The process of electrospinning has given the field of tissue engineering insight into many aspects of tissue engineered scaffolds, including how factors such as fiber diameter and porosity are affected by polymer concentration. However, the affects of fiber alignment upon the material properties of electrospun scaffolds remains unclear. The purpose of this study is to determine how the material properties of electrospun gelatin scaffolds are affected by changes in fiber alignment and starting gelatin concentration. Gelatin scaffolds, with starting concentrations of 80, 100, and 130mg/m1, were electrospun onto a target mandrel rotating at various speeds. Samples of each scaffold were …