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Biomedical Engineering and Bioengineering Commons™

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Selected Works

2002

Articles 1 - 3 of 3

Full-Text Articles in Biomedical Engineering and Bioengineering

Functional Profiling Of The Saccharomyces Cerevisiae Genome, Patrick Flaherty Jul 2002

Functional Profiling Of The Saccharomyces Cerevisiae Genome, Patrick Flaherty

Patrick Flaherty

Determining the effect of gene deletion is a fundamental approach to understanding gene function. Conventional genetic screens exhibit biases, and genes contributing to a phenotype are often missed. We systematically constructed a nearly complete collection of gene-deletion mutants (96% of annotated open reading frames, or ORFs) of the yeast Saccharomyces cerevisiae. DNA sequences dubbed 'molecular bar codes' uniquely identify each strain, enabling their growth to be analysed in parallel and the fitness contribution of each gene to be quantitatively assessed by hybridization to high-density oligonucleotide arrays. We show that previously known and new genes are necessary for optimal growth …

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Isoform-Specific Stimulation Of Cardiac Na/K Pumps By Nanomolar Concentrations Of Glycosides., Glenn Gaudette, Junyuan Gao, Randy Wymore, Yongli Wang, Irvin Krukenkamp, Ira Cohen, Richard Mathias Mar 2002

Isoform-Specific Stimulation Of Cardiac Na/K Pumps By Nanomolar Concentrations Of Glycosides., Glenn Gaudette, Junyuan Gao, Randy Wymore, Yongli Wang, Irvin Krukenkamp, Ira Cohen, Richard Mathias

Glenn R. Gaudette

It is well-known that micromolar to millimolar concentrations of cardiac glycosides inhibit Na/K pump activity, however, some early reports suggested nanomolar concentrations of these glycosides stimulate activity. These early reports were based on indirect measurements in multicellular preparations, hence, there was some uncertainty whether ion accumulation/depletion rather than pump stimulation caused the observations. Here, we utilize the whole-cell patch-clamp technique on isolated cardiac myocytes to directly measure Na/K pump current (I(P)) in conditions that minimize the possibility of ion accumulation/depletion causing the observed effects. In guinea pig ventricular myocytes, nanomolar concentrations of dihydro-ouabain (DHO) caused an outward current that appeared …

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Deconvolution Of Compartmental Water Diffusion Coefficients In Yeast-Cell Suspensions Using Combined T1 And Diffusion Measurements, Matthew D. Silva, Karl G. Helmer, Jing-Huei Lee, Sam S. Han, Charles S. Springer, Christopher H. Sotak Dec 2001

Deconvolution Of Compartmental Water Diffusion Coefficients In Yeast-Cell Suspensions Using Combined T1 And Diffusion Measurements, Matthew D. Silva, Karl G. Helmer, Jing-Huei Lee, Sam S. Han, Charles S. Springer, Christopher H. Sotak

Sam Han

Deconvolution of Compartmental Water Diffusion Coefficients in Yeast-Cell Suspensions Using Combined T1 and Diffusion Measurements

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