Engineering Commons^™

Purification And Characterization Of The Prostate Cancer-Associated Antigen: 7e11-C5, Qi Feng

Theses and Dissertations in Biomedical Sciences

A murine monoclonal antibody (MAb), 7E11-C5, recognizes a prostate organ-specific antigen. Under reducing conditions, the 7E11-C5 antigen exhibited major 100-Kd and minor 70-Kd bands, suggesting that the antigen comprises two peptide chains. The antigen was detected in pooled normal human seminal plasma, xenograft tissue extract of a prostate adenocarcinoma cell line, LNCaP, and tissue extracts of benign prostatic hyperplasia (BPH) and prostate carcinomas, but was not identified in any of the non-prostate tissue extracts tested. Antibodies of the three major prostate-associated antigens (prostatic acid phosphatase (PAP), prostate specific antigen (PSA) and prostate secretory protein (PSP)) were unable to block the …

1-Nitronaphthalene Causes Ultrastructural Changes Primarily In Nonciliated Bronchiolar Epithelium Followed By Ciliated Epithelium In The Rat, John-Michael Sauer

Masters Theses

This study describes morphological changes in the lung at several time intervals following a single injection of 1-Nitronaphthalene (100 mg/kg intraperitoneal) in male Sprague-Dawley rats using transmission and scanning electron microscopy. Dilation of the smooth endoplasmic reticulum and mitochondrial distension in the nonciliated bronchiolar (Clara) cells was apparent as early as 1 hour after injection, while adjacent ciliated cell showed minimal changes. At 6 hours, when respiratory distress was apparent in the animals, Clara and ciliated cells exhibited chromatin clumping, loss of mitochondrial cristae and cytoplasmic vacuolization. Also at 6 hours, the interstitial area around the terminal bronchioles was infiltrated …

Metabolism Of Arachidonate-Containing Phospholipid Molecular Species In The Murine Macrophage-Like Cell Line, P388d1, Crystal R. Waites

Electronic Theses and Dissertations

Glycerophospholipids of mammalian cells exist as chemically diverse structures with various fatty acids at the sn-1 and sn-2 positions. Arachidonic acid, a polyunsaturated fatty acid, which may be converted to biologically active eicosanoids such as prostaglandins, thromboxanes, and leukotrienes, is found predominantly in the sn-2 position of glycerophospholipids. The purpose of this study was to examine, at the level of the individual molecular species, the incorporation of arachidonate into phospholipids and its release from phospholipids during stimulation. In this way, the specificity of the enzymes controlling arachidonate metabolism could be examined in order to clarify the processes that control the …

Development And Characterization Of Monoclonal Antisperm Antibodies: Potential For Contraception, Dilrowshan H. Haque

Theses and Dissertations in Biomedical Sciences

There is an expressed need to develop a greater variety of safe contraceptive methods which would find acceptance worldwide, particularly in developing countries. Immunization against spermatozoa might be such a method as judged by the accumulated evidence from studies on human females and in a number of female animal species.

Two extraction techniques were used for sperm membrane antigen isolation. The first technique involved NP-40 detergent for antigen extraction from human motile sperm and the second technique employed homogenized human testis for antigen extraction. Using these immunogens, sperm membrane-specific monoclonal antibodies (MAbs) were developed. When these antisperm MAbs were subjected …

The Effects Of Shading By Open-Pile Structures On The Density Of Spartina Alterniflora, Heather L. Mcguire

Dissertations, Theses, and Masters Projects

No abstract provided.

Electromagnetic Field Variations In The Activation Of Bone Cell Synthesis, Gary Ketcherside

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract Not Included]

Thermal Desorption Of Hazardous And Toxic Organic Compounds From Soil Matrices : Dichloromethane, Chloroform, Benzene, Toluene, 1-Chloronaphthalene, 1, 2, 4-Trichlorobenzene, Arun S. Chemburkar

Theses

This research project was undertaken to understand the process of desorption/removal of organic compounds from soil matrices under the influence of temperature and gas purging. The project was divided in two parts :

A. Plug deposition experiments: Columns packed with soil matrices were purged with an inert gas under isothermal conditions. Organic compounds were then introduced as a plug on the front of the column by on-line injection. The rate of passage (due to sequential adsorption/desorption) of the plug through the various soil matrices was measured by a gas chromatograph equipped with a flame ionization detector (FID). The compounds studied …

A Simple Solid-Phase Electrophoretic Procedure For The Separation Of Plasmid Dna, Linda Ann Simurra

Biological Sciences Theses & Dissertations

A method was developed for extraction of plasmid DNA from bacterial cells embedded in agarose blocks. Cell - containing blocks were treated with various lysing reagents and inserted into the wells of an agarose gel. Upon electrophoresis the plasmid DNA migrated out of the embedding block and into the gel leaving intact chromosomal DNA in the well. This method was tested with various organisms and found to be effective for plasmid isolation. In comparison to "traditional" procedures, this new method is less tedious since chemical separation of plasmids is not required prior to electrophoresis. Also, a higher yield of plasmid …

Steady-State Operation Of Bench-Scale Activated Sludge Process Using Phenol And 2-Chlorophenol As Substrates, Jeffrey Richard Caputi

Theses

Experiments were conducted to determine whether a continuous process similar to the common activated sludge process could be operated at a relative steady state with phenol and 2-chlorophenol as the sole carbon sources. After a number of unsuccessful trials, process conditions were developed which allowed for operation at a relative steady state with respect to the concentration of microorganisms in the reactor and the concentration of phenol and 2-chlorophenol in the effluent.

Phenol and 2-chlorophenol were removed at better than 99 percent efficiency on a continuous basis. The mixed liquor grown under the conditions of the experiment was highly filamentous …

Microencapsulation As An Alternative To Emulsification For Preparing As A For Use In Papermaking, William J. Corriveau

Masters Theses

The sizing chemical ASA is emulsified to make i t compatible with the papermaking process. Unfortunately, emulsified ASA is chemically reactive with water at the elevated temperatures found in papermaking systems. As an alternative, encapsulated ASA was made and investigated for its stability towards water at elevated temperatures.

A handsheet procedure was developed to analyze emulsified and encapsulated ASA. It was concluded that the procedure gave statistically reproducible results. Investigations with the procedure revealed important variables concerning the laboratory and m ill application of ASA. Evidence indicated that retention of ASA was critical to sizing development.

Encapsulated ASA made for …

The Kinetics And Hepatotoxicity Of Benzyl Chloride Vapor In Rats, Hari V. Rao

Theses

Male Sprague Dawley rats (250g) were exposed to benzyl chloride vapor at a concentration of 220 ppm for 6 hours. During inhalation, the blood benzyl chloride concentration increased to reach a steady state level of 9-10 ug/ml between 4 and 6 hours of exposure. A small concentration of benzyl chloride (0.86 ug/ml) was present in the blood four hours after the termination of exposure. The absorption half life was 0.53 hours (0.39-0.81) and the elimination half life was l.12 hour (0.93-l.40).

The absorbed benzyl chloride was largely retained in the blood and some of it (0.22 ug/g) was sequestered into …

Scheduling Effects Of 5-(3,3-Dimethyl-1-Triazeno)Imidazole-4-Carboxamide (Dtic) And 1,3-Bis-Chloroethyl-1-Nitrosourea (Bcnu) On Mouse Melanoma Cells B16cl5, Gracie V. Coffey

Theses

DTIC and BCNU have been demonstrated to have enhanced chemotherapeutic effects in mice bearing Bl6 mouse melanoma cells. The order of administration seems to be important in maintaining optimum effects. This was shown is a study by Hill et al. (unpublished).

In the present study, mouse melanoma cells, Bl6CL4, were treated with either DTIC or BCNU. The drug was allowed to remain on the cells for 2, 3, or 4 days. The drug-containing media were then removed, the cells were washed and fresh medium was added. The cells were incubated in an atmosphere of 5% CO2 and 95% air at …

Protective Laboratory Measures For The Flexible Wall Permeameter And The Atterberg Limits Tests Using Hazardous And Toxic Chemicals, Robert A. Tetla

Theses

Safety procedures for performing geotechnical testing with hazardous and toxic materials, excluding radioactive wastes, are developed for the Flexible Wall Permeameter and the Atterberg Limits Tests. The tests were evaluated in detail to determine the critical points at which the technician would be exposed to the hazardous and/or toxic permeant. It was found that the following safety measures should always be taken; 1) the apparatus and all testing should be undertaken in a sliding sash hood using the proper face velocity, 2) air monitoring using a personal dosimeter and/or a detector tube is required to assure that the concentration of …

Microbial Characteristics Of An Activated Sludge And Three Commercial Preparations After Exposure To Phenolics, Laurie Gneiding

Theses

The biological characteristics of four microbial mixtures were examined after successive exposure to phenol and 2-chlorophenol at room temperature. One of the microbial mixtures was an activated sludge from the Livingston, NJ municipal wastewater treatment plant. The other three mixtures were commercial preparations sold by Polybac Corp.(Hydrobac), Sybron Corp. (BI-CHEM DC 1006/1007), and General Environmental Sciences(LLMO).

The characteristics examined were relative counts and species of gram negative/gram positive bacteria, yeast and fungi, protozoa, and higher organisms. For the gram negative bacteria and yeasts, culturing techniques and diagnostic tubes were used to determine the predominant species. Biochemical tests were done on …

Investigations Into Rates And Mechanisms Of Mercury Depuration In The Killifish, Fundulus Heteroclitus, Joanne A. Korpanty

Theses

Metallothionein (MT) and glutathione (GSH) have been shown in mammalian research to play a role in the sequestration and depuration of mercury. The sulfhydryl groups on both these peptides have a strong affinity for mercury, MT for Hg²⁺ and GSH for CH₃Hg⁺. In this study, killifish (Fundulus heteroclitus) were force-fed one piece of squid laced with labelled mercuric nitrate to give a final food concentration of 20 ppm ²⁰³Hg²⁺ and 0.03 -0.05 µg g-1 Hg²⁺ total dose. Most of the mercury, 84.1 ± 12.1% (mean ± standard deviation), is depurated within the …

Analysis Of Selected Volatile Organic Substances Associated With Residential Kerosene Heater Use And The Health Implications, Catherine Coombs Bobenhausen

Theses

Volatile organic species (aliphatic hydrocarbons from hexane to dodecane, cyclohexane and aromatic species including benzene, toluene, ethyl benzene, m,p, and o—xylenes, nitrobenzene, styrene and naphthalene) associated with residential kerosene heater emissions were measured at two sites. One was a small room having a total volume of 23.66 cubic meters and an air change rate of 2.71 air changes per hour, and the other, a living/dining room area having a combined volume of 79.30 cubic meters and an estimated air change rate of .7 air changes per hour. The analytical procedure involved collection of a series of air samples in stainless …

Acrylamide Effects On Nerve Terminal Enzymes, Christopher S. E. Marlowe

Theses

Acrylamide causes a peripehral neuropathy. The biochemical mechanism by which it does so is the subject of intense investigation. One of the principal hypotheses for this mechanism centers on acrylamide's ability to inhibit glycolysis. This study examines the effects of in vivo exposure to acrylamide on the enzymes of the nerve terminal particle, the synaptosome.

A method for measuring glycolytic enzyme inhibition in the synaptosome was developed. Acrylamide (500 mg/kg) was administered to rats intraperitoneally. Synaptosomes were prepared by the method of Dodd et al, and lysed by sonication. GAPDH activity and NSE concentration were measured. GAPDH inhibition measured in …

Petroleum Degradation By Naturally Occurring Populations Of Marine Bacteria From Middle Atlantic Outer Continental Shelf Waters, Alexander Maccubbin

Dissertations, Theses, and Masters Projects

Bacterial populations indigenous to surface (1 m) waters of the Middle Atlantic Outer Continental Shelf were sampled at seasonal intervals to determine the abundance and distribution of petroleum-degrading (HC) and "total" heterotrophic (HET) bacteria. Simultaneously, unweathered South Louisiana crude oil (SLCO) was added to aliquots of surface water samples to evaluate rates and patterns of degradation of hydrocarbon. Degradation was studied using selected nutrient and temperature regimes in both closed flask and prototype "open" or continuous dilution systems. HET bacterial levels generally decreased with distance from land (x inshore = 6 x 10('4) MPN/ml to x offshore = 6 x …

Spectral Signature Studies For Application In Deer Census Using Remote Sensing Techniques, Maran C. Pate

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study was performed to determine the spectral signatures of deer and their natural background elements for censusing purposes. Consideration was given to atmospheric transmittance, acceptable flying weather, and terrain. Possible spectral bands between 0.3 and 14.0 μm were obtained (over a pathlength of 1500 feet at an altitude of 5000 feet) based upon atmospheric transmittance using the LOWTRAN 3B computer program. They are: 0.30 - 1.33, 1.49 - 1.79, 2.00 - 2.50, 3.00 - 3.16, 3.38 - 4.10, 4.59 - 5.05, and 8.00 - 13.33 μm, for transmittance greater than 75%. Weather conditions are favorable for flying and taking …

The Effects Of A Kinesthetic Training Program On The Acquisition Of Industrial Skills Among University Students, John V. Mockler

Dissertations and Theses @ UNI

Training workers for specific jobs is receiving much attention in industry. The demand for skilled workers is increasing constantly and as this happens newer and more efficient methods of training are sought In the psychomotor/sensory area. skills that appear to offer a wide amount of transferability are those dealing with fine sensitivity, manual dexterity, and coordination. Even though these skills lend themselves well to training, this area is somewhat deficient in current educational and training programs (Sjogren, 1977, p.23). Presently, on-the-job training is widely used for training workers in the actual tasks they will perform during their job.

The process …

Discharge Measurement And Energy Efficiency Evaluation Of Irrigation Pumping Plants, Bruce A. Jennings

Electronic Theses and Dissertations

No information has been available regarding efficiencies of electric irrigation pumping plants in South Dakota. Pumping plant discharge, a key parameter in the calculation of pumping plant efficiency, is often difficult to measure in the field. A project was initiated at South Dakota State University in 1976 to investigate field pumping plant efficiencies. The following objectives were established for the project. 1. To investigate various methods of measuring irrigation pumping plant discharge. 2. To develop a suitable field procedure for determining electric irrigation pumping plant efficiency. 3. To measure energy efficiency of selected electric irrigation pumping plants in South Dakota.

Performance Of A Diurnally Tracking Solar Energy-Intensifier, Calvin E. Siegel

Electronic Theses and Dissertations

The United States agricultural system provides numerous opportunities in which solar energy can be utilized. Low to moderate temperature rise collectors can provide a large percentage of the energy requirements normally met by using high quality fossil fuels. As world demand for food production increases due to a growing population, alternative sources of energy need to be developed and applied as substitutes for these increasingly scarce fossil fuels. Agriculture offers practical advantages for the use of solar heat energy because there exists sufficient area for solar collectors and a wide range of temperature applications on most farmsteads. Demand for energy …

Bacterial Response To Crude Oil Spillage In A Salt Marsh, Russell Paul Herwig

Dissertations, Theses, and Masters Projects

No abstract provided.

Determination Of Lead Content In Apical Tissue Following Root Canal Obturation With Sargenti's Formula, G. Merle Hickok

Loma Linda University Electronic Theses, Dissertations & Projects

Four Stump-tail monkeys were used to help determine if the lead in Sargenti's formula diffuses out into the apical tissue while confined entirely within the root canal. In each animal two teeth were filled with Sargenti's formula 1-2 millimeters short of the radiographic apex, and two teeth were filled with gutta percha and Proco-Sol sealer for controls, making a total sample size of sixteen. The time ranged from 48 hours to 120 days. Lead analysis using the spectrophotometric method showed a higher lead content in the teeth filled with Sargenti's formula than with the gutta percha controls.

Drip And Sprinkler Irrigation Of Carrots And Onions, James D. Melstad

Electronic Theses and Dissertations

Many specialty crops, especially vegetables, are shallow rooted and sensitive to periods of moisture stress. This makes irrigation of vegetables necessary to their production in South Dakota since summer rainfall is normally both inadequate and undependable. A study was initiated in conjunction with the South Dakota Agricultural Experiment Station and the Water Resources Institute to evaluate irrigation of specialty crops in eastern South Dakota. Drop and sprinkler irrigation were utilized for the irrigation of two specialty crops presently grown in South Dakota, carrots and onions. The study was initiated with the following objectives: 1. To compare carrots and onion yields …

Integrated Numerical Simulation Of Drip Irrigation With Subsurface Drainage, James Ray Hoover

Electronic Theses and Dissertations

A numerical method and model were developed to simulate soil moisture during steady-state and transient flow toward a tile drain. Intermittent application of water by drip irrigation and rainfall were simulated by use of finite difference approximation equations. No known analytical solution for these flow situations exists because of the complex mathematics describing unsaturated flow in the soil. The computer model is based on the electrical resistance network analog. The explicit finite difference equations were solved by the successive over-relaxation method for steady-state flow and by iteration for transient soil moisture flow. The computer program for the model consists of …

An Evaluation Of In-Stream Structures Designed To Provide Fish Habitat, Charles H. Call Jr.

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In-stream concrete structures were studied through model tests and river tests. The model studies indicated that four designs provided good habitat in the model stream. These structures were the inverted weir, the "V" structure, the slab with legs and the cylinder. Through the river studies it was determined that these structures did not influence enough of the total river area to be effective in providing good fish habitat. Also an appreciable amount of yearly maintenance would be required to free the structures from silting in, debris, and vandalism. The slab with legs was the only promising structure.

A Method Of Analyzing Mandibular Movement, Richard B. Mccoy

Loma Linda University Electronic Theses, Dissertations & Projects

Mandibular movement is considered to be a fundamental factor in the study, analysis, and treatment of occlusion. For many years, dentists have realized that dental health depends upon the compatibility of mandibular movements and occlusion. This realization has led to the development of new methods and techniques for recording accurately and scientifically the movements of the mandible. In order for this data to be of scientific value, it must be stored in a usable form which is easily available for analysis.

The purpose of this research project was to develop a procedure for storing and recalling mandibular-movement information. Before such …

Evaluation Of Bi-Level Drainage Theory With A Viscous-Flow Analog, James Marfred Kienholz

Electronic Theses and Dissertations

The primary purpose of this study is to evaluate the validity of this bi-level theory with a viscous-flow analog. Viscous-flow analogs have been used by many investigators in the past to evaluate analytical solution to drainage problems (Bear, 1972). The objectives of this study are: 1. Design and construct a viscous-flow analog for laboratory and classroom use, 2. Test the reliability of existing bi-level drainage theory with the analog, and 3. Provide design data for use in the solution of bi-level drainage problems. The bi-level drainage theory will be graphically presented for design purposes provided the theory is shown to …

X-Ray-Induced Specific-Locus Mutation Rates In Newborn And Young Mice, Paul Bruce Selby

Doctoral Dissertations

The specific-locus mutation frequency resulting from 300 R of acute X irradiation has been determined for the germ cells present in male mice at 0, 2, 4, 6, 8, 10, 14, 21, 28, and 35 days of age and also for female mice at 0 days of age, Sample size was much larger for the males irradiated on day 0 than for other age groups but in all groups it was large enough to insure that an extremely high rate would be noticed. At 35 days of age the testis is histologically similar to that of the adult. It was …