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Short Food Supply Chains: Expectations And Reality, Richard Roberto Richards Jan 2015

Short Food Supply Chains: Expectations And Reality, Richard Roberto Richards

Graduate College Dissertations and Theses

Alternative food systems (AFSs) are so defined because they purport to challenge a value or ameliorate a negative impact of the dominant conventional food system (CFS). Short food supply chains (SFSCs) are a type of AFS whose alterity is defined by socially proximal economic exchanges that are embedded in and regulated by social relationships. This relational closeness is argued to have benefits with respect to economic, environmental, and social sustainability. However, it would be a mistake to assume that AFSs and CFSs are paradigmatically differentiated or that their structures engender particular outcomes.

The first article traces a misguided attempt to …


Supporting A Growing Agricultural Economy By Understanding Child Care In Farm Families, Emily Stengel Jan 2015

Supporting A Growing Agricultural Economy By Understanding Child Care In Farm Families, Emily Stengel

Graduate College Dissertations and Theses

This thesis argues for the consideration of child care accessibility and costs as one factor in the success and wellbeing of farmers in the United States. There is a long tradition in rural studies of recognizing that farms are not just economic enterprises but are family-based social enterprises as well, with household level issues and family roles that are both acknowledged and contested. However, child care is missing from virtually all scholarly and public discussions of agricultural workforce development - even more so than other social services and family supports. Additionally, the agricultural sector, considered as a portion of U.S. …


The Common Cause Of All Advanced And Progressive Mankind: Proletarian Internationalism, Spain, And The American Communist Press, 1936 - 1937, G. Scott Waterman Jan 2015

The Common Cause Of All Advanced And Progressive Mankind: Proletarian Internationalism, Spain, And The American Communist Press, 1936 - 1937, G. Scott Waterman

Graduate College Dissertations and Theses

In July 1936, units of the Spanish military, backed by a collection of domestic right-wing elements and by fascist governments elsewhere in Europe, staged a rebellion against the legally constituted national government that had been elected five months previously. The governing bloc, an ideologically broad coalition of liberal republicans, Marxists, and anarchists known as the People's Front, embodied the strategy formulated by Stalin and the Communist International (Comintern) in Moscow to stem the advance of international fascism and mitigate the danger it posed to the Soviet Union and, by extension, the communist movement and the global radical working class it …


An Embodied Approach To Evolving Robust Visual Classifiers, Karol Zieba Jan 2015

An Embodied Approach To Evolving Robust Visual Classifiers, Karol Zieba

Graduate College Dissertations and Theses

From the very creation of the term by Czech writer Karel Capek in 1921, a "robot" has been synonymous with an artificial agent possessing a powerful body and cogitating mind. While the fields of Artificial Intelligence (AI) and Robotics have made progress into the creation of such an android, the goal of a cogitating robot remains firmly outside the reach of our technological capabilities. Cognition has proved to be far more complex than early AI practitioners envisioned. Current methods in Machine Learning have achieved remarkable successes in image categorization through the use of deep learning. However, when presented with novel …


Polycystin-2 (Pkd2), Eccentric (Xnta), And Meckelin (Mks3) In The Ciliated Model Organism Paramecium Tetraurelia, Megan Smith Valentine Jan 2015

Polycystin-2 (Pkd2), Eccentric (Xnta), And Meckelin (Mks3) In The Ciliated Model Organism Paramecium Tetraurelia, Megan Smith Valentine

Graduate College Dissertations and Theses

Paramecium tetraurelia is a ciliated single cell used as a model organism for the study of ciliopathies. Ciliopathies are mammalian diseases involving the dysfunction of cilia, including cilia maintenance, construction, and signaling. P. tetraurelia and its cilia provides an excellent non-canonical system for the investigation and elucidation of proteins important for the structure, maintenance and function of cilia and ciliary beating. We utilize features of this cell such as its 1000's of cilia and highly organized and patterned cell surface to observe changes in swimming behavior or disruptions in the ordered cell surface which are not feasible in mammalian cells. …


The Role Of A Surface Glycoprotein In The Interaction Of Aggregatibacter Actinomycetemcomitans With Dendritic Cells, Yan Xing Jan 2015

The Role Of A Surface Glycoprotein In The Interaction Of Aggregatibacter Actinomycetemcomitans With Dendritic Cells, Yan Xing

Graduate College Dissertations and Theses

Periodontal diseases are a group of prevalent inflammatory diseases affecting the tissues supporting and surrounding the teeth. In periodontal diseases, the inflammatory response of the periodontal tissues is induced by a polymicrobial biofilm formed at or below the gingival margin. Aggregatibacter actinomycetemcomitans, a Gram-negative oral pathogen identified within this biofilm, is a causative agent of both chronic and localized aggressive periodontitis. Dissemination of A. actinomycetemcomitans from the oral cavity also initiates multiple systemic infections, including soft tissues abscesses, pneumonia and endocarditis.

The innate immune response is the first line of defense against bacterial infections. Dendritic cells, a group of professional …


Nazi Collaborators, American Intelligence, And The Cold War: The Case Of The Byelorussian Central Council, Mark Alexander Jan 2015

Nazi Collaborators, American Intelligence, And The Cold War: The Case Of The Byelorussian Central Council, Mark Alexander

Graduate College Dissertations and Theses

When the military forces of the Third Reich invaded the Soviet Union in June 1941, the German authorities used local anti-Communist collaborators to facilitate the invasion and the occupation of the conquered territories. Many of these Byelorussian collaborators became complicit in the perpetration of the Holocaust and eagerly created a puppet regime under the direct control of the Schutzstaffel (SS). However, this regime and the crimes of its members remain largely unknown.

As the Third Reich crumbled, the members of the SS-sponsored Byelorussian Central Council (BCC) hid themselves in the confusion of postwar Europe’s Displaced Persons camps, where they began …


Measuring The Impacts Of A School Garden-Based Nutrition Intervention, Jenna Banning Jan 2015

Measuring The Impacts Of A School Garden-Based Nutrition Intervention, Jenna Banning

Graduate College Dissertations and Theses

School garden-based education programs have become an increasingly popular tool to improve children's nutrition, yet evaluations have found mixed results. This study analyzes three years of student surveys collected before and after one year of participation in the American Heart Association's Teaching Gardens program. Analysis was guided by the Social Cognitive Theory, and measured changes in determinants of healthy eating behavior: preferences for fruits and vegetables, gardening skills, food systems learning, and perceptions of self-efficacy and social norms regarding fruit and vegetable consumption. A total of 755 pre-test and 976 post-test responses were included in the analysis, as well as …


The Impact Of Engagement With Community Supported Agriculture On Human Attitude Towards The Sustainable Food Movement, Ariana Margarita Cano Jan 2015

The Impact Of Engagement With Community Supported Agriculture On Human Attitude Towards The Sustainable Food Movement, Ariana Margarita Cano

Graduate College Dissertations and Theses

With a total of 164 community supported agriculture programs (CSA), Vermont is leading the "locavore" movement in the United States, ranked number one in the country with the most CSAs and Food Hubs per capita. ("Locavore Index," 2013)

CSAs have a large positive impact on reducing carbon emissions, advancing local economic growth, and promoting healthy lifestyles of consumers. The purpose of this study is to explore the overall experience of individuals in comparison to their current social norm, individual attitudes, identity, and intentions of change, and understand any change overtime in their individual attitude and behavior.

Attitude change was measured …


Investigation Of A Ring Fragmentation Reaction For The Synthesis Of Tethered Aldehyde Ynones And Medium Sized Cyclic Ynones And Ynolides, Ali Bayir Jan 2015

Investigation Of A Ring Fragmentation Reaction For The Synthesis Of Tethered Aldehyde Ynones And Medium Sized Cyclic Ynones And Ynolides, Ali Bayir

Graduate College Dissertations and Theses

The fragmentation of γ-silyloxy-β-hydroxy-α-diazoesters to provide tethered aldehyde ynoates was discovered and developed in Prof. Brewer's laboratory. This reaction is a Lewis acid mediated heterolytic cleavage of the Cβ-γ bond of a γ-silyloxy-β-hydroxy-α-diazocarbonyl functional group array contained in a ring compound.

This dissertation describes a further study of this ring fragmentation reaction and application of this fragmentation to the preparation of synthetically useful organic molecules. The purpose of this dissertation work was three fold. The first objective was to extend this ring fragmentation reaction to the synthesis of tethered aldehyde ynones by fragmenting various γ-silyloxy-β-hydroxy-α-diazo ketone compounds. The second objective …


A Comparative Examination Of The Safety Programs At Ucla, Umn, And Uvm In Response To Recent Chemistry Laboratory Incidents, Victoria Carhart Jan 2015

A Comparative Examination Of The Safety Programs At Ucla, Umn, And Uvm In Response To Recent Chemistry Laboratory Incidents, Victoria Carhart

Graduate College Dissertations and Theses

Laboratory safety has recently become more of an imperative in research laboratories than it has ever been in the past. Recent accidents at several universities have escalated the awareness of safety concerns in laboratory workspaces among the general public and created a greater need for a stronger culture of safety in chemistry research overall. Historically, results and publications have been the top priority of most researchers, not laboratory safety.

This thesis discusses a number of laboratory accidents. The first happened in December of 2008 at the University of California, Los Angeles (UCLA) and resulted in the death of a graduate …


Ubiquitin Ligase Trim32 And Chloride-Sensitive Wnk1 As Regulators Of Potassium Channels In The Brain, Eugene Miler Cilento Jan 2015

Ubiquitin Ligase Trim32 And Chloride-Sensitive Wnk1 As Regulators Of Potassium Channels In The Brain, Eugene Miler Cilento

Graduate College Dissertations and Theses

The voltage-gated potassium channel Kv1.2 impacts membrane potential and therefore excitability of neurons. Expression of Kv1.2 at the plasma membrane (PM) is critical for channel function, and altering Kv1.2 at the PM is one way to affect membrane excitability. Such is the case in the cerebellum, a portion of the brain with dense Kv1.2 expression, where modulation of Kv1.2 at the PM can impact electrical activity of neurons and ultimately cerebellum-dependent learning. Modulation of Kv1.2 at the PM can occur through endocytic trafficking of the channel; however mechanisms behind this process in the brain remain to be defined.

The goal …


A Conserved Inner Membrane Protein Of Aggregatibacter Actinomycetemcomitans Is Integral For Membrane Function, Kenneth Smith Jan 2015

A Conserved Inner Membrane Protein Of Aggregatibacter Actinomycetemcomitans Is Integral For Membrane Function, Kenneth Smith

Graduate College Dissertations and Theses

The cell envelope of Aggregatibacter actinomycetemcomitans, a Gram-negative pathogenic bacterium implicated in human oral and systemic disease, plays a critical role in maintenance of cellular homeostasis, resistance to external stress, and host'pathogen interactions. Our laboratory has identified a novel gene product, morphogenesis protein C (MorC), deletion of which leads to multiple pleotropic effects pertaining to membrane structure and function. The MorC sequence was determined to be conserved in Gammaproteobacteria. Based on this bioinformatic analysis, the functional conservation of this protein was investigated utilizing an A. actinomycetemcomitans morC mutant as a model system to express homologs from four phylogenetically diverse representatives …


Sustainable Agriculture In Vermont: Economics Of Climate Change Best Management Practices And The Complexity Of Consumer Perceptions Of Raw Milk, Alexander Paul Helling Jan 2015

Sustainable Agriculture In Vermont: Economics Of Climate Change Best Management Practices And The Complexity Of Consumer Perceptions Of Raw Milk, Alexander Paul Helling

Graduate College Dissertations and Theses

Changing weather patterns, the declining social fabric of rural communities, and economic uncertainty increasingly pose challenges to Vermont communities. The socially and environmentally embedded production practices within sustainable agriculture present a potential solution to these problems. In order to make the most of the potential benefits of these practices society must maximize their adoption. This requires an understanding of both farmer adoption of these practices and consumer perceptions of the resulting food products. This thesis contributes two original articles on sustainable agriculture through the analysis of factors driving both farmer adoption and consumer perceptions of products and practices often thought …


Examining Two Green Payment Options To Support Dairy Farm Viability In Northern New England: Anaerobic Digestion And Organic Production, Deborah Ann Krug Jan 2015

Examining Two Green Payment Options To Support Dairy Farm Viability In Northern New England: Anaerobic Digestion And Organic Production, Deborah Ann Krug

Graduate College Dissertations and Theses

This thesis explores the details and profitability of two distinct operational strategies utilized by dairy operations as alternatives to expanding milk production. It features farms that have either transitioned to organic production or installed a farm-scale anaerobic digester, motivated in part by the opportunity for market specialization or income diversification to increase the viability of their dairy farm businesses.

The first analysis examines the demographics and production characteristics impacting the profitability of organic dairy farmers in Vermont and Maine. This provides policymakers, educators, lenders, and suppliers with a profile of this sector that accounts for 23% of dairy farms in …


A Sustainability Themed K-5 Magnet School: An In-Depth Case Study And Evaluation, Aleidria R. Lichau Jan 2015

A Sustainability Themed K-5 Magnet School: An In-Depth Case Study And Evaluation, Aleidria R. Lichau

Graduate College Dissertations and Theses

This thesis is a descriptive case study of the first sustainability magnet themed elementary school in the U.S., the Sustainability Academy (SA) in Burlington, VT. The thesis provides an in depth case study narrative of the first pilot year of the SA (2009) as well as an examination of the analytical frameworks for evaluating the efficacy of sustainability movements in schools.

A literature review is provided to distinguish between a type of static sustainability that supports the current educational paradigm and a type of sustainable education that identifies sustainability as a moving target and a deep process to transcend the …


The Supply Chain Of Fair Trade Coffee: Challenges, Opportunities & The Future Inside A Troubled Industry, Katharine D. Lukas Jan 2015

The Supply Chain Of Fair Trade Coffee: Challenges, Opportunities & The Future Inside A Troubled Industry, Katharine D. Lukas

Graduate College Dissertations and Theses

What started as a grassroots effort to aid tradespeople in developing nations, Fair Trade and similar certification models have, over the last sixty years, successfully established themselves as a viable alternative to conventional international trade; the ongoing growth of their market share and volume emphasize the increasing market demand for these alternatives. For coffee, Fair Trade's oldest and most established commodity, over two billion pounds was sold as certified in 2012 alone and the percentage of certified coffee continues to grow in share each year (Volcafe, 2012, Fair Trade USA 2012). As Fair Trade continues to grow, so does the …


Intuitions Or Informational Assumptions? An Investigation Of The Psychological Factors Behind Moral Judgments, Nolan Rampy Jan 2015

Intuitions Or Informational Assumptions? An Investigation Of The Psychological Factors Behind Moral Judgments, Nolan Rampy

Graduate College Dissertations and Theses

There is an ongoing debate among psychologists regarding the psychological factors underlying moral judgments. Rationalists argue that informational assumptions (i.e. ideological beliefs about how the world works) play a causal role in shaping moral judgments whereas intuitionists argue that informational assumptions are post hoc justifications for judgments made automatically by innate intuitions. In order to compare these two perspectives, the author conducted two studies in which informational assumptions related to ingroups and outgroups varied across conditions. In Study 1, political conservatives and liberals completed the moral relevance questionnaire while imagining they were in the US, Iran, or no specific country. …


Using Mutli Criteria Decision Analysis To Develop Sustainability Assessment Tools: Biomass Supply Chains, Deandra Marie Perruccio Jan 2015

Using Mutli Criteria Decision Analysis To Develop Sustainability Assessment Tools: Biomass Supply Chains, Deandra Marie Perruccio

Graduate College Dissertations and Theses

Energy access remains a significant challenge in nations lacking access to resources and strong infrastructure systems, creating barriers to economic development and to increased standards of living. Small scale biomass gasification energy (BGE) systems have been developed to meet energy needs in rural areas, creating synergies between agricultural and agro-forestry systems through utilization of biomass feedstock for energy generation. The sustainability of such systems requires sophisticated planning and coordination of the biomass supply chain.

The goal of this thesis is to investigate and improve structural and process related characteristics of sustainability assessments for small scale bio-energy systems, specifically focusing on …


The Application Of Valid And Feasible Dietary Assessment Methods To Evaluate The Impact Of Policy And Behavioral Interventions On Children's Fruit And Vegetable Consumption, Sarah Anne Amin Jan 2015

The Application Of Valid And Feasible Dietary Assessment Methods To Evaluate The Impact Of Policy And Behavioral Interventions On Children's Fruit And Vegetable Consumption, Sarah Anne Amin

Graduate College Dissertations and Theses

The majority of U.S. children do not meet recommendations for fruit and vegetable (FV) consumption. FV confer numerous health benefits including intake of critical shortfall nutrients, reduced risk of chronic diseases, and maintenance of healthy weights. Identifying both policy and behavioral approaches to increase children's consumption of a variety of FVs has become a public health priority. When evaluating policy and interventions it is integral to apply validated dietary assessment methods to measure FV selection, consumption, and waste. The three aims of this dissertation addressed the feasibility of dietary assessment methods and their application to evaluate policy change and behavioral …


The Role Of Estrogen In Emotional And Cognitive Processes Integral To Major Depressive Disorder, Kimberly Albert Jan 2015

The Role Of Estrogen In Emotional And Cognitive Processes Integral To Major Depressive Disorder, Kimberly Albert

Graduate College Dissertations and Theses

Women have greater incidence and prevalence of Major Depressive Disorder (MDD) than men during the reproductive life phase when ovarian hormones fluctuate, suggesting that ovarian hormones have a significant role in MDD etiology in women. As the core symptoms of MDD are indicative of alterations in stress responding, emotional processing, and mood regulation, examining the effects of the estrogen on these processes in women may provide a better understanding of the role of estrogen in the sex difference in MDD rates. The general aim of this dissertation was to examine neural, emotional, and attentional processes related to stress response alterations …


Phosphate Removal And Recovery From Wastewater By Natural Materials For Ecologically Engineered Wastewater Treatment Systems, Daniel Thomas Curran Jan 2015

Phosphate Removal And Recovery From Wastewater By Natural Materials For Ecologically Engineered Wastewater Treatment Systems, Daniel Thomas Curran

Graduate College Dissertations and Theses

Eutrophication due to excess loading of phosphorus (P) is a leading cause of water quality degradation within the United States. The aim of this study was to investigate P removal and recovery with 12 materials (four calcite varieties, wollastonite, dolomite, hydroxylapatite, eggshells, coral sands, biochar, and activated carbon. This was accomplished through a series of batch experiments with synthetic wastewater solutions ranging from 10-100 mg PO₄-P/ L. The results of this study were used to establish large-scale, calcite-based column filter experiments located in the Rubenstein School of Environment and Natural Resources' Eco-Machine. Influent and effluent wastewater samples were routinely collected …


Quantification Of The Impact Of Intermittent Renewable Penetration Levels On Power Grid Frequency Performance Using Dynamic Modeling, Elizabeth Ann Kirby Jan 2015

Quantification Of The Impact Of Intermittent Renewable Penetration Levels On Power Grid Frequency Performance Using Dynamic Modeling, Elizabeth Ann Kirby

Graduate College Dissertations and Theses

As the technology behind renewable energy sources becomes more advanced and cost-effective, these sources have become an ever-increasing portion of the generation portfolios of power systems across the country. While the shift away from non-renewable resources is generally considered beneficial, the fact remains that intermittent renewable sources present special challenges associated with their unique operating characteristics. Because of the high variability of intermittent renewables, the frequency performance of the system to which they are connected can degrade. Generators assigned to regulate frequency, keeping it close to the desired 60 Hz, are forced to ramp up and down quickly in order …


A Randomized Pilot Trial: An Internet-Based Mind/Body Intervention To Mitigate Anxiety In Women Experiencing Infertility, Jessica Clifton Jan 2015

A Randomized Pilot Trial: An Internet-Based Mind/Body Intervention To Mitigate Anxiety In Women Experiencing Infertility, Jessica Clifton

Graduate College Dissertations and Theses

Infertility is a frequently occurring chronic health condition, which often persists throughout the reproductive years. Heightened anxiety symptoms often are comorbid with infertility diagnoses. Women experiencing infertility, and particularly those with anxiety symptoms, characterize an emerging population that deserves special attention. However, women experiencing infertility have identified barriers to seeking psychotherapy (e.g., fears of being dismissed from fertility treatment and/or stigmatized). Consequently, women diagnosed with infertility need a psychotherapy that not only can reduce these symptoms, but can also be private and convenient. The current study translated an empirically tested in-person mind/body protocol into an internet-based intervention to suit the …


Toward An Understanding Of The Breakdown Of Heat Transfer Modeling In Reciprocating Flows, Ian Pond Jan 2015

Toward An Understanding Of The Breakdown Of Heat Transfer Modeling In Reciprocating Flows, Ian Pond

Graduate College Dissertations and Theses

Reynolds average Navier-Stokes (RANS) modeling has established itself as a critical design tool in many engineering applications, thanks to its superior computational efficiency. The drawbacks of RANS models are well known, but not necessarily well understood: poor prediction of transition, non-equilibrium flows, mixing and heat transfer, to name the ones relevant to our study. In the present study, we use a direct numerical simulation (DNS) of a reciprocating channel flow driven by an oscillating pressure gradient to test several low- and high-Reynolds' RANS models. Temperature is introduced as a passive scalar to study heat transfer modeling. Low-Reynolds' models manage to …


Teenagers' Mode Choice To And From School And Technology Use For Transportation: Analysis Of Students From Five High Schools In Vermont And California, Paola Rekalde Aizpuru Jan 2015

Teenagers' Mode Choice To And From School And Technology Use For Transportation: Analysis Of Students From Five High Schools In Vermont And California, Paola Rekalde Aizpuru

Graduate College Dissertations and Theses

The carhops and drive-ins of the 1950s are symbolic of the freedom that the automobile has granted Americans. What the general public has gained from the automobile, however, may come at the expense of independent mobility and choices for today's adolescents, particularly those not yet old enough to drive or those from lower income families. Sprawl land use development patterns and limited transportation choices in most American cities often hold teenagers and their chauffeuring parents captive to the automobile. At the same time, information and communication technology is fast evolving and changing the ways in which teenagers live, interact, and …


From Dar Es Salaam To Dartmouth: A Case Study Of The Experiences Of Fogarty Aids International Training And Research Program Fellows At Dartmouth College, Lisa Anne Purvis Jan 2015

From Dar Es Salaam To Dartmouth: A Case Study Of The Experiences Of Fogarty Aids International Training And Research Program Fellows At Dartmouth College, Lisa Anne Purvis

Graduate College Dissertations and Theses

From Dar es Salaam to Dartmouth: A Case Study of the Experiences of Fogarty AIDS International Training and Research Program Fellows at Dartmouth

Lisa Purvis, EdD Candidate, MPH, MBA

Background

The United States (US) is a major host nation to international college students and scholars who study a variety of disciplines (Farrugia & Bhandari, 2014). Beginning in the last decade, the demand for global health training has risen (Kanter, 2008; Kerry, Ndung'u, Walensky, Lees, Kayanjas, & Bangsberg, 2011).

Since 2000, Geisel School of Medicine at Dartmouth has been one of 26 US universities participating in a global health training program, …


Becoming Transdisciplinary: Exploring Process In A Research Initiative On Climate Change, Emil Tsao Jan 2015

Becoming Transdisciplinary: Exploring Process In A Research Initiative On Climate Change, Emil Tsao

Graduate College Dissertations and Theses

The subject of this case study is the Vermont Agricultural Resilience in a Changing Climate initiative, a transdisciplinary research team at UVM that has maintained success in meeting research and outreach objectives despite collaborating in a way that does not follow any particular ideal-type transdisciplinary process. In following recent science and technology (STS) studies' accounts of cross-disciplinary collaboration, the hypothesis pursued is that the transdisciplinary study of messy or "wicked" problems like climate change brings forth an array of responses from researchers whose disciplinary backgrounds already position them to pursue their research differently, particularly when they involve outside stakeholders in …


Grass-Based Dairy In Vermont: Benefits, Barriers, And Effective Public Policies, Serge William Wiltshire Jan 2015

Grass-Based Dairy In Vermont: Benefits, Barriers, And Effective Public Policies, Serge William Wiltshire

Graduate College Dissertations and Theses

A comprehensive literature review was undertaken in order to define and assess the sustainability and resiliency characteristics associated with grass-based and confinement dairy farming. Primarily as a result of reduced input costs, grass-based dairy farming often enhances profitability over confinement systems, especially on small farms. Further, conversion of tilled soil to permanent pasture has been shown to significantly reduce harmful sediment and nutrient transport into waterways. Perennial forage also acts as a carbon sink, curtailing or even negating a grass-based farm's carbon footprint. Finally, social benefits derived from enhanced nutrition and higher quality of life are also associated with grass-based …


Analysis Of Function Of The Son1-Interacting Protein, Lnk2 In Arabidopsis Thaliana, Prince Kudjoe Zogli Jan 2015

Analysis Of Function Of The Son1-Interacting Protein, Lnk2 In Arabidopsis Thaliana, Prince Kudjoe Zogli

Graduate College Dissertations and Theses

The Arabidopsis SON1 F-box protein was implicated in regulating a pathogen defense pathway, but its exact function in wild-type plants is unknown. As an F-box protein it was predicted that SON1 would assembles into a SON1-SCF ubiquitin ligase complex that recruits specific plant defense-related proteins for proteolysis. The yeast 2-hybrid assay was used to screen for potential substrates for a putative SON1-SCF ligase, leading to the identification of Arabidopsis LNK2 as a SON1-binding factor.

Comprehensive protein-protein interaction analysis has shown that the binding of SON1 to LNK2 protein is specific, because closely related, full-length Arabidopsis F-box proteins do not interact …