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Articles 1561 - 1566 of 1566
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The Construction Of A Mammalian Transfectionvector For Expression Of Cytosine-5 Specific Dnamethyltransferase Gene M.Msp1 In Cultured Cells, Öztürk Özdemi̇r
The Construction Of A Mammalian Transfectionvector For Expression Of Cytosine-5 Specific Dnamethyltransferase Gene M.Msp1 In Cultured Cells, Öztürk Özdemi̇r
Turkish Journal of Biology
The expression vectors are designed for expression and purification of normal or recombi-nant gene of interest. There is a wide variety of stable and transferable selectable mammalian expres-sion vectors.The vector pCDM8 and its derivatives, pcDNAI/Ampicilline are widely used for cloning and analyzing of genes in higher eukaryotic cells. The vectors pRC/CMV, pcDNA3 and pRC/RSV are designed for high-level expression of recombinant genes in mammalian cells. In the present study, we have been able to transfect and express the monospecific bacterial (Moroxcella spp) cytosine-5 DNA methyltransferase (MTase) M.Msp1 gene in cultured cells within a newly con-structed mammalian transfection vector. We have …
Biochemical Characterization Of Elastase Frompseudomonas Aeruginosa Ses 938-1, Semra Kocabiyik, Ezgi Ergi̇n
Biochemical Characterization Of Elastase Frompseudomonas Aeruginosa Ses 938-1, Semra Kocabiyik, Ezgi Ergi̇n
Turkish Journal of Biology
We have investigated some biochemical properties of elastase from a new strain o f Pseudomonas aeruginosa (SES 938-1). Activities were measured at 410 nm using N-succinyl-L-(ala) 3 -p- nitroanilide as substrate. The elastase activity followed Michaelis-Menten kinetics over the substrate range of 0.067-0.540 mM with the apparent K m value of 0.375 mM. Optimum activity was observed at 36 °C and pH 7.5. elastase activity was inhibited by metal chelating agents and high concentrations of Mn 2+ , Zn 2+ and Ni 2+ . The results obtained suggest that elastase from P. aeruginosa SES 938-1 is a neutral metalloproteinase.
Serological Properties Of Pseudomonas Syringaepvphaseolicolaisolates Collected From Eskisehir, Dilek Ertugrul, Kıymet Güven
Serological Properties Of Pseudomonas Syringaepvphaseolicolaisolates Collected From Eskisehir, Dilek Ertugrul, Kıymet Güven
Turkish Journal of Biology
Thirty six bean seed samples collected from bean fields in Eskisehir-Türkiye were examined for detection and isolation of Pseudomonas syringae pv. phaseolicola. A polyclonal antiserum specific to P. s. pv. phaseolicola NCPPB 52 was raised in a rabbit and both biochemical and serological test were used for identification. Heat-stable O antigens of 92 isolates identified as P. S. pv. phaseolicola together with authentic isolates of the bacterium were compared by Ouchterlony immunodiffusion test and all the isolates had a common antigenic structure.
The Effects Of Phenoxybenzamine And Cold Stresson Tyrosine Hydroxylase Enzyme Activity Andexpression Of Tyrosine Hydroxylase Gene In The Ratadrenal Medulla, Muhittin Yürekli
The Effects Of Phenoxybenzamine And Cold Stresson Tyrosine Hydroxylase Enzyme Activity Andexpression Of Tyrosine Hydroxylase Gene In The Ratadrenal Medulla, Muhittin Yürekli
Turkish Journal of Biology
The effects of cold exposure and cold plus phenoxybenzamine treatment on tyrosine hydrox-ylase (TH) activity and TH m RNA levels were investigated in the adrenal medulla of Sprague Dawley (SD) rats. SD rats were exposed to cold (4 °C) for 48 hours. Phenoxybenzamine was injected intra-peritoneally (i.p) at 20 mg/kg, which was prepared in 5% ethanol containing 0.9 % NaCL TH activity was measured by monitoring the formation of 3 H 2 O as a product of L-(3 H)-tyrosine hydroxylation, the formation of L-(3 H)-dopa from L-(3 H)-tyrosine. Total adrenal RNA was isolated and blotted onto a nylon membrane and …
A Theoretical Study On The Sex-Pheromones Of Therice Moth, Corcyra Cephalonica Stainton, Lemi Türker
A Theoretical Study On The Sex-Pheromones Of Therice Moth, Corcyra Cephalonica Stainton, Lemi Türker
Turkish Journal of Biology
Molecular orbital calculations (AM1 type) were performed on the geometry optimized structures of various farnesal isomers and methyl esters of farnesoic acids. It has been found that the major component, E, E-farnesal, of the sex-pheromone for the rice moth, Corcyra cephalonica Stainton is chemically the most exothermic and stable of all the farnesal isomers. Then, the resem-blance of molecular shapes was detrmined in order to design more stable sex-attractants.
Larval Growth And Survival Of Penaeus Indicus(Decapoda: Penaeidae) On Live Feeds, Metin Kumlu
Larval Growth And Survival Of Penaeus Indicus(Decapoda: Penaeidae) On Live Feeds, Metin Kumlu
Turkish Journal of Biology
This study was conducted to compare the suitability of three microalgal species (Tetraselmis chuii, Skeletonema costatum and Rhinomonas reticulata), both individually and in combination, as food for Penaeus indicus during larval culture. To determine the best feeding density, each alga was fed to protozoea 1 (PZI) larvae from 10 to 80 cells mL -1 . The best algal-cell density, promoting the highest survival rate, greatest growth and fastest larval development, was obtained at 60-70 cells mL -1 with a combination of T. chuii and S. costatum. Low cell densities (10-20 cells mL -1 ) were not effective. The diatom S. …