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Prenatal Exposure Of Diclofenac Sodium Alters The Behavioral Development Of Young Wistar Rats, Bi̇rsen Eli̇bol, Begüm Oğur, Havva Doğru
Prenatal Exposure Of Diclofenac Sodium Alters The Behavioral Development Of Young Wistar Rats, Bi̇rsen Eli̇bol, Begüm Oğur, Havva Doğru
Turkish Journal of Biology
Diclofenac sodium (DS), a potent inhibitor of cyclooxygenase, reduces the release of arachidonic acid and formation of prostaglandins. Being a nonsteroid drug that shows antiinflammatory action, the possible side effects of fetal DS administration gain importance in public and medical applications. Herein, the effects of DS administration (1 mg/kg) during gestational days 5-20 were investigated on the performance of Wistar rat pups in a variety of behavioral tasks. Four-week-old pups were subjected to sensory motor tests, a plus maze, an open field, the Morris water maze, and a radial arm maze. Fetal DS disrupted some sensory motor performances, such as …
Decellularization Of Rat Adipose Tissue, Diaphragm, And Heart: A Comparison Of Two Decellularization Methods, Meli̇s Sesli̇, Esi̇n Akbay, Mehmet Ali̇ Onur
Decellularization Of Rat Adipose Tissue, Diaphragm, And Heart: A Comparison Of Two Decellularization Methods, Meli̇s Sesli̇, Esi̇n Akbay, Mehmet Ali̇ Onur
Turkish Journal of Biology
Decellularization is a process that involves the removal of cellular material from the tissues and organs while maintaining the structural, functional, and mechanical properties of extracellular matrix. The purpose of this study was to carry out decellularization of rat adipose tissue, diaphragm, and heart by using two different methods in order to compare their efficiency and investigate proliferation profiles of rat adipose-tissue-derived mesenchymal stem cells (AdMSCs) on these scaffolds. Tissues were treated with an optimized detergent-based decellularization (Method A) and a freeze-and-thaw-based decellularization (Method B). AdMSCs were then seeded on scaffolds having a density of 2 × 105 cells/scaffold and …
High Dietary Fructose Load Aggravates Lipid Metabolism In The Liver Of Wistar Rats Through Imbalance Between Lipogenesis And Fatty Acid Oxidation, Ana Teofilovic, Biljana Bursac, Ana Djordjevic, Danijela Vojnovic Milutinovic, Gordana Matic, Natasa Velickovic
High Dietary Fructose Load Aggravates Lipid Metabolism In The Liver Of Wistar Rats Through Imbalance Between Lipogenesis And Fatty Acid Oxidation, Ana Teofilovic, Biljana Bursac, Ana Djordjevic, Danijela Vojnovic Milutinovic, Gordana Matic, Natasa Velickovic
Turkish Journal of Biology
Fructose ingestion is often associated with hepatic steatosis and hypertriglyceridemia. The homeostasis of hepatic lipids is mainly determined by the interplay of lipogenesis and fatty acid β-oxidation. In this study, we hypothesized that high fructose intake disturbs hepatic lipid metabolism through an imbalance between these processes. Therefore, we analyzed the effects of a 9-week-long consumption of a 60% fructose solution on physiological parameters, glycemia, and blood lipid profiles in male Wistar rats. The expression of key regulators of fatty acid oxidation (FAO) and lipogenesis in the liver were assessed by western blot and quantitative polymerase chain reaction. The results showed …
Comparison Of The Osteogenic Differentiation Capacity Of Adipose Tissuederived Mesenchymal Stem Cells From Humans And Rats, Seçi̇l Erden Tayhan, Şeyma Taşdemi̇r, Sai̇me İsmet Deli̇loğlu Gürhan, Erol Mi̇r
Comparison Of The Osteogenic Differentiation Capacity Of Adipose Tissuederived Mesenchymal Stem Cells From Humans And Rats, Seçi̇l Erden Tayhan, Şeyma Taşdemi̇r, Sai̇me İsmet Deli̇loğlu Gürhan, Erol Mi̇r
Turkish Journal of Biology
Mesenchymal stem cells (MSCs) can be found in many types of adult tissues such as the bone marrow, adipose, placenta, liver, and periosteum. Recently, adipose tissue-derived MSCs (ADMSCs) have become one the most preferred MSC types because of their fast proliferation rate, abundance, and high plasticity for variable cell types. It is known that ADMSCs are able to differentiate into various cells, including osteoblasts, so they are quite promising for orthopedic clinical trials. The present study aimed to compare the osteogenic differentiation conditions of MSCs from human adipose tissue (hADMSC) and those of MSCs from rat adipose tissue (rADMSC). Therefore, …
Amelioration Of Subchronic Acrylamide Toxicity In Large Intestine Of Rats Byorganic Dried Apricot Intake, Mehmet Erman Erdemli̇, Zümrüt Doğan, Yilmaz Çi̇ğremi̇ş, Müslüm Akgöz, Eyüp Altinöz, Murat Geçer, Yusuf Türköz
Amelioration Of Subchronic Acrylamide Toxicity In Large Intestine Of Rats Byorganic Dried Apricot Intake, Mehmet Erman Erdemli̇, Zümrüt Doğan, Yilmaz Çi̇ğremi̇ş, Müslüm Akgöz, Eyüp Altinöz, Murat Geçer, Yusuf Türköz
Turkish Journal of Biology
Acrylamide (AA) has neurotoxic, mutagenic, and genotoxic effects in humans and experimental animals. Fruit consumption is important for human health, because fruits are the source of many nutrients such as vitamins, minerals, carotenoids, dietary fiber, and phytonutrients. Many agricultural products provide natural melatonin in the diet. At the onset of the study, rats were weighted and randomly divided into four groups each containing 10 rats as follows: group 1: control (fed with normal diet and normal drinking water); group 2: apricot (fed with a daily diet with 5% apricot and normal drinking water); group 3: AA (administered daily acrylamide at …
Melatonin Increased Vitamin C And Antioxidant Enzyme Values In The Plasma,Heart, Liver, And Kidney Of Adriamycin-Treated Rats, Ali̇ Zi̇ya Karakilçik, Muharrem Bi̇ti̇ren, Mustafa Zeri̇n, Haki̇m Çeli̇k, Nurten Aksoy
Melatonin Increased Vitamin C And Antioxidant Enzyme Values In The Plasma,Heart, Liver, And Kidney Of Adriamycin-Treated Rats, Ali̇ Zi̇ya Karakilçik, Muharrem Bi̇ti̇ren, Mustafa Zeri̇n, Haki̇m Çeli̇k, Nurten Aksoy
Turkish Journal of Biology
Adriamycin, an anticarcinogenic agent, causes excessive production of reactive oxygen species (ROS). These radicals may affect antioxidant defense systems. The present study was designed to investigate the effects of melatonin on antioxidant enzyme activities and vitamin C in Adriamycin-treated rats. In the current study, rats were divided into three groups: control, Adriamycin, and melatonin+Adriamycin groups. The control group was intraperitoneally injected physiological saline (0.9%) as a placebo. The Adriamycin and melatonin+Adriamycin groups were administered Adriamycin (25 mg/kg body weight). The melatonin+Adriamycin group was pretreated with melatonin (0.5 mg/kg body weight). Vitamin C concentration and catalase (CAT), superoxide dismutase (SOD), glutathione …
Melatonin Is A Potent Modulator Of Antioxidative Defense And Cellular Proliferation Against Aluminum Toxicity In Rats, Ni̇hal Ömür Bulan, Guner Sarikaya-Unal, Sevim Tunali, Pelin Arda Pi̇ri̇nççi̇, Refiye Yanardag
Melatonin Is A Potent Modulator Of Antioxidative Defense And Cellular Proliferation Against Aluminum Toxicity In Rats, Ni̇hal Ömür Bulan, Guner Sarikaya-Unal, Sevim Tunali, Pelin Arda Pi̇ri̇nççi̇, Refiye Yanardag
Turkish Journal of Biology
Aluminum is the most abundant metal on earth, known for its toxicity to humans and laboratory animals. Melatonin, produced by the pineal gland, has been shown to be an effective antioxidant. In this study, we investigated the possible protective role of melatonin in modulating toxicity and oxidative stress induced by chronic exposure to aluminum in the small intestine of rats. Wistar albino rats were divided into five groups. Group I served as the control and received only physiological saline, Group II served as the control for the melatonin group and received ethanol and physiological saline, Group III received melatonin, Group …
L-Arginine Protects Against Ethylene Glycol-Induced Gastric Mucosal Damage In Rats: Immunohistochemical And Electron Microscopic Study, Samah Kandeel, Thoryaa El_Deeb, Ehsan Salah, Nafisa El-Bakary, Naglaa Sarhan, Mohamed Balaha, Hiroki Sakai, Tokuma Yanai
L-Arginine Protects Against Ethylene Glycol-Induced Gastric Mucosal Damage In Rats: Immunohistochemical And Electron Microscopic Study, Samah Kandeel, Thoryaa El_Deeb, Ehsan Salah, Nafisa El-Bakary, Naglaa Sarhan, Mohamed Balaha, Hiroki Sakai, Tokuma Yanai
Turkish Journal of Biology
Ethylene glycol is an alcohol widely used in industry as an antifreeze. It affects the normal gastric mucosal defense mechanisms, inducing surface ulcerations. L-arginine is a precursor of nitric oxide that exhibits multiple biological properties; among them is the acceleration of wound healing, especially in the stomach. Caspase-3 immunohistochemistry, and semithin and ultrathin section examinations were used to investigate the possible protective effect of L-arginine on ethylene glycol-induced gastric mucosal damage in rats. Three animal groups were used: group A (control group), group B (received 3.3 mL/kg ethylene glycol for 2 weeks), and group C (received 200 mg/kg of L-arginine …
The Effect Of 3-Methylcholanthrene And Butylated Hydroxytoluene On Glycogen Levels Of Liver, Muscle, Testis, And Tumor Tissues Of Rats, Fi̇kri̇ye Polat, Egemen Dere, Eylem Gül, İzzet Yelkuvan, Öztürk Özdemi̇r, Günsel Bi̇ngöl
The Effect Of 3-Methylcholanthrene And Butylated Hydroxytoluene On Glycogen Levels Of Liver, Muscle, Testis, And Tumor Tissues Of Rats, Fi̇kri̇ye Polat, Egemen Dere, Eylem Gül, İzzet Yelkuvan, Öztürk Özdemi̇r, Günsel Bi̇ngöl
Turkish Journal of Biology
This study examined the effects of separate and combined applications of 3-methylcholanthrene, a polycyclic aromatic hydrocarbon and potent carcinogenic agent, and butylated hydroxytoluene, the antioxidant food additive, on the glycogen levels of liver, muscle, testis, and tumor tissues in rats. Adult male Wistar albino rats weighing 100-110 g at 8 weeks of age were used in this study. This study consisted of a control group (n = 9) and 3 different experiment groups in which rats were chronically treated with 3-methylcholanthrene (n = 9) or butylated hydroxytoluene (n = 11) or a combination of these agents (n = 14). Rats …
The Protective Effect Of Boric Acid On Aluminum-Induced Hepatotoxicity And Genotoxicity In Rats, Hasan Türkez, Fati̇me Geyi̇koğlu, Suat Çolak
The Protective Effect Of Boric Acid On Aluminum-Induced Hepatotoxicity And Genotoxicity In Rats, Hasan Türkez, Fati̇me Geyi̇koğlu, Suat Çolak
Turkish Journal of Biology
The efficacy of boric acid (BA) was examined on liver marker enzymes in aluminum (Al)-treated rats. Also, a liver micronucleus assay was performed to evaluate the genotoxicity in hepatocytes. With these aims, Sprague-Dawley rats were randomly separated into 10 groups of 5 animals. Aluminum chloride (5 mg/kg body weight (b.w.) AlCl3) and BA (3.25, 13, 36 and 58.5 mg/kg b.w.) alone were administered with injections to the experimental animals. Furthermore, the animals were also treated with Al for 4 consecutive days followed by BA exposure for 10 days. The rats were anesthetized after Al and BA injections and the levels …
Stimulatory Effects Of Ghrelin On Spontaneous Contractions In The Rat Myometrium, Alpaslan Dayangaç, Seli̇m Kutlu, Vahi̇t Konar
Stimulatory Effects Of Ghrelin On Spontaneous Contractions In The Rat Myometrium, Alpaslan Dayangaç, Seli̇m Kutlu, Vahi̇t Konar
Turkish Journal of Biology
Ghrelin, a recently isolated hormone, has been reported to have modulatory effects on smooth muscle contractility. In this study, we investigated the effects of ghrelin on spontaneous contractions in the rat uterus in vitro. Myometrium strips were removed from Wistar rats following decapitation and placed in a jacketed tissue bath. After initiation of spontaneous contractions, control contractions were recorded for 10 min and various concentrations of ghrelin were added to the tissue bath cumulatively. The Wilcoxon signed ranks test was used for statistical analysis. Application of ghrelin augmented the spontaneous contractions in myometrial strips. The mean peak amplitudes of contractions …
The Genotoxic And Antigenotoxic Effects Of Aloe Vera Leaf Extract In Vivo And In Vitro, Ahmet Kayraldiz, Ayşe Yavuz Kocaman, Eyyüp Rencüzoğullari, Erman Sali̇h İsti̇fli̇, Hasan Basri̇ İla, Mehmet Topaktaş, Yusuf Kenan Dağlioğlu
The Genotoxic And Antigenotoxic Effects Of Aloe Vera Leaf Extract In Vivo And In Vitro, Ahmet Kayraldiz, Ayşe Yavuz Kocaman, Eyyüp Rencüzoğullari, Erman Sali̇h İsti̇fli̇, Hasan Basri̇ İla, Mehmet Topaktaş, Yusuf Kenan Dağlioğlu
Turkish Journal of Biology
The genotoxic and antigenotoxic effects of Aloe vera leaf extract (AV) were investigated using the chromosome aberrations (CAs) test for the bone marrow cells of rats, sister chromatid exchanges (SCEs) and micronucleus (MN) and CAs tests for human lymphocytes, and the Ames Salmonella/microsome test system. In the bone marrow cells of rats, AV extract significantly induced structural and total CAs at all concentrations and in all treatment periods. In human peripheral lymphocytes, AV did not increase the mean SCE; however, it significantly induced the MN frequency and structural CAs. In addition, AV showed a cytotoxic effect by decreasing the replication …
Ultrastructural Changes In Rat Liver By Methyleugenol And Evaluation Of Some Biochemical Parameters, Ayşegül Çerkezkayabeki̇r, Gülnur Kizilay, Fi̇gen Ertan
Ultrastructural Changes In Rat Liver By Methyleugenol And Evaluation Of Some Biochemical Parameters, Ayşegül Çerkezkayabeki̇r, Gülnur Kizilay, Fi̇gen Ertan
Turkish Journal of Biology
The present study investigated the effect of methyleugenol, a food flavoring and fragrance agent, on the livers of laboratory rats. Doses of 10 and 30 mg/kg/day of methyleugenol (in 0.5% methylcellulose) were administered intragastrically (IG) to 2 dose groups for 10 days alongside a control group (n = 10 for each group). Gains in body weight were not statistically significant for either dose. Lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) activities decreased (P < 0.05) for both dose groups, but AST (aspartate aminotransferase) activity decreased significantly (P < 0.05) only in the 30 mg/kg/day methyleugenol dose group. Alanine aminotransferase (ALT) activity did not change significantly in either group. The total amount of glucose remained unchanged, but glycogen in the liver decreased significantly (P < 0.05) in the 30 mg/kg/day methyleugenol dose group. There was also an increase of swelling in the smooth endoplasmic reticulum sacs (sER), electron dense accumulations in the sER, cytoplasmic vacuolization, a slight increase of mitochondria and lysosomes, and invaginations in the nucleus membranes of hepatocytes. Extensions in bile canaliculi and increasing microvilli of bile canaliculi in the liver were also observed. These findings indicate the ultrastructural toxic effect of methyleugenol on rat livers in 10 and 30 mg/kg/day doses. Therefore, we may speculate that the toxic effect of methyleugenol needs to be examined in detail.
Effects Of Subchronic Treatment Of Some Plant Growth Regulators On Serum Enzyme Levels In Rats, İsmai̇l Çeli̇k, Hanefi̇ Özbek, Yasi̇n Tülüce
Effects Of Subchronic Treatment Of Some Plant Growth Regulators On Serum Enzyme Levels In Rats, İsmai̇l Çeli̇k, Hanefi̇ Özbek, Yasi̇n Tülüce
Turkish Journal of Biology
The effects of a sublethal concentration of three plant growth regulators (PRGs) on serum enzymes in rats were investigated under laboratory conditions. 100-ppm of PRGs, indoleacetic acid (IAA), indolebutiric acid (IBA) and kinetin were administered orally to 8 rats ad libitum for 3 weeks. The hormon treatments caused different effects on the level of serum alanin aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), amylase and creatine phosphokinase (CPK) in comparison to those of control rats. According to the results, while the levels of LDH and CPK were increased significantly by IBA, the levels of AST, LDH and CPK were …
Immunohistochemical Distribution Of Transforming Growth Factor Alpha,Laminin, Fibronectin And Desmin In Rat Ovary, Gökhan Akkoyunlu, İsmai̇l Üstünel, Ramazan Demi̇r
Immunohistochemical Distribution Of Transforming Growth Factor Alpha,Laminin, Fibronectin And Desmin In Rat Ovary, Gökhan Akkoyunlu, İsmai̇l Üstünel, Ramazan Demi̇r
Turkish Journal of Biology
Since the pure synthesis of transforming growth facto alpha (TGF\alpha) many attempts have been made in order to determine its distribution in various tissues. As a result of the complexitiy of the cyclic periods throughout experimental models, it is not easy to determine the distribution of TGF\alpha in the system. Especially the pre-and through-gestational pattern of TGF\alpha in rat ovary has not been reported fully. Furthermore, the shortage of literature about intracellular desmin and laminin, fibronectin of different intermediate filaments whether they have any interaction with TGF\alpha through this period, makes the subject worth discussing. Immunocytochemical techniques were utilized in …
Humerus Primer Ossification Zone Development In Rat Fetuses: A Histological And Histochemical Study, İsmai̇l Üstünel, Gökhan Akkoyunlu, Erdoğan Kocamaz, Ramazan Demi̇r
Humerus Primer Ossification Zone Development In Rat Fetuses: A Histological And Histochemical Study, İsmai̇l Üstünel, Gökhan Akkoyunlu, Erdoğan Kocamaz, Ramazan Demi̇r
Turkish Journal of Biology
In this investigation of the primary ossification zones of 14-18 day-old fetal rat humeri by histological, histochemical and histoenzymatical methods, 30 rat fetuses of the species Rattus norvegicus were used. The humeral diaphysis samples of the fetuses were subjected to alcian blue-heamatoxylin, periodic acid Schiff (PAS)-heamatoxylin, alcian blue-PAS-heamatoxylin, alizarin red and toluidine blue, alkaline phosphatase (ALP), adenosine triphosphatase (ATPase) and acid phophatase (ACP) staining techniques. The results can be summarized as follows: On day 14, no ossification signs were evident yet, whereas on day 15, the initiation of degenerations in some chondrocyte cytoplasms and the formation of osteoblastic cells on …
Combined Effects Of Endosulfan, Dimethoate And Carbaryl On Serumcalcium Levels And Heart Muscle Of Rats, Güldeniz Özmen, E. Arzu Elcüman
Combined Effects Of Endosulfan, Dimethoate And Carbaryl On Serumcalcium Levels And Heart Muscle Of Rats, Güldeniz Özmen, E. Arzu Elcüman
Turkish Journal of Biology
In this study, combination of low [acceptable daily intake (ADI)] and high (10xADI) doses of endosulfan, dimethoate and carbarly were administrated orally to the male rats for 3.5 months. Serum calcium levels of rats in all groups were determined. At the same time, whether or not there was calcification in heart muscle of rats in all groups were investigated histochemically. Serum calcium levels of rats treated with high dose of endosulfan+carbaryl and dimethoate+carbaryl mixture decreased significantly in comparison with controls. Calcification was not found in heart muscle tissues of the rats in all groups according to the results of histochemical …