Forensic Science and Technology Commons^™

The Role Of Dna Exoneration In The Bloodsworth Case, John T. Brasfield

SACAD: Scholarly Activities

In the Kirk Bloodsworth case, a man was falsely convicted for the rape and murder of Dawn Hamilton. He spent eight years in prison after being convicted through witness testimony, where he learned about DNA profiling. After submitting a request to check his DNA against the DNA left at the scene, he was exonerated. Later, the DNA left at the scene was checked against a database and got a hit on a convicted rapist who had been charged shortly after Mr. Bloodsworth. If there had been the same access to DNA technology during the trial or if they had listened …

Overcoming The Effects Of Oily Contaminants On Dna Extraction Efficiency Using Dna Iq™ System, Elizabeth G. Gillece

Master of Science in Forensic Science Directed Research Projects

In forensic science DNA extraction and purification are crucial to DNA analysis as evidentiary items vary in DNA quantity and quality. In casework the goal to is maximize DNA yield, remove PCR inhibitors, and develop a full profile while preserving evidence. Extraction methods are developed to target the DNA for optimal isolation. Solid-phase extraction is advantageous as it can extract DNA from a variety of tissue types, it is amenable to automation, and it provides quick and efficient purification of DNA. Solid-phase extraction methods may have complications with contaminants that hinder the quantity and quality of the DNA recovered. In …

Evaluation Of Cytokeratin-Specific Antibody And Autofluorescent Signals As Predictors Of Time Since Deposition For Saliva Samples, Dillon Ryder

Master of Science in Forensic Science Directed Research Projects

In a forensic investigation, STR profiles generated from biological evidence can frequently be informative to identify an individual or provide an investigative lead. However, STR typing does not provide information to answer activity-level questions which can be highly important to a criminal case, such as the Time Since Deposition (TSD) of biological evidence. Determining the relative time in which a biological sample was left at a crime scene would provide additional information regarding the timeline of a crime and a person of interest’s relationship to the scene. One proposed method of estimating TSD is to use flow cytometry to analyze …

Determination Of The Trace Elemental Signature Of Drywall Dust And Its Implication As Forensic Evidence, Emilee Holmes

Master of Science in Forensic Science Directed Research Projects

Drywall is found in most homes built in the United States of America because it is cost effective, light weight, and easy to install. Its brittle nature makes it less resistant to impact which can cause dust to form if a force is exerted on it. Airborne dust can settle on anything or anyone around it and can be considered trace evidence for its ability to connect suspects, victims, and scenes; however, this type of dust is not emphasized as trace evidence and is not always collected due to a lack of understanding of their forensic signatures.The seven producers of …

Evaluation Of Emerging Screening Technologies For The On-Site Detection And Identification Of Methamphetamine And Its Precursors In Simulated Clandestine Lab Operations, Alexis Nicole Wilcox

Graduate Theses, Dissertations, and Problem Reports (ETD)

Stimulant drugs comprise one of the top drug categories abused in the United States. Due to its accessibility, low price, and manufacturing simplicity, methamphetamine is frequently placed within the top 10 seized drugs in the country. As of March 2023, methamphetamine is the most seized controlled substance in the United States, with 34,291 kg. In 2022, the United States seized over 79,000 kg of methamphetamine. One reason for the proliferation of methamphetamine is related to the production itself, which does not require large warehouses but can be manufactured in houses using relatively accessible materials and small containers. When a clandestine …

Bci Validation: Yfiler Plus Kit Validation, Chloe Koon

Honors Projects

In order to have access and use the Combined DNA Index System (CODIS), it is required by the Federal Bureau of Investigation's Quality Assurance Standards (FBI-QAS) for Forensic DNA Units to complete comprehensive validation studies on all equipment, materials, and methods used in the process of DNA analysis. This study continues validation previous done within the Ohio Bureau of Criminal Investigation (BCI). YFiler Plus is a DNA amplification kit that is used in cases where it is most useful to examine only male DNA profile(s) within a sample by amplifying loci only on the Y-Chromosome. Components of this study include …

Using A Non-Destructive Method To Estimate The Number Of Contributors And Predict Dna Yield In Touch Dna Mixtures, Christin Lee

Master of Science in Forensic Science Directed Research Projects

Touch DNA samples are low templates and often are mixtures, making them typically one of the most difficult types of evidence to interpret. Short tandem repeat analysis is a standard method to estimate the number of contributors and DNA yield for each contributor; however, there are some limitations. Applying a non-destructive pre-DNA analysis technique that provides information about the touch DNA may improve the casework process. Antibody probes to hormones such as testosterone and dihydrotestosterone (DHT), and proteins including cytokeratins (CK) inside epidermal cells, have been demonstrated to produce a personalized signature for human touch cell populations. Testosterone, DHT, and …

Assessment Of Quechers Extraction Protocol For The Lc-Ms/Ms Detection Of Emerging Fentanyl Analogs In Biological Specimens For Clinical And Forensic Purposes, Kylea M. Morris

Graduate Theses, Dissertations, and Problem Reports (ETD)

Drug abuse within the United States is a dynamic and pervasive issue that affects the quality and life expectancy of our society. One of the major drug classes that are constantly utilized is opioids. Opioids are prescribed for pain management in patients suffering from cancer and other medical illnesses. Long-term use of opioids could lead to tolerance in humans, which poses a risk of reaching fatal overdose levels. Unfortunately, opioids are abused recreationally, and in some instances, individuals are unaware of the presence of dangerous synthetic drugs like fentanyl, hidden in materials such as heroin, cocaine, oxycodone, and methamphetamine. Recent …

Performance Of A Dna And Protein Co-Extraction With Trypsin In Comparison To Two Proteinase K Based Dna Extraction Methods, Dinura A. Gunatilake

Student Theses

Effective DNA extraction methods are important for forensic applications. The main goal of this experiment was to determine if a newly developed trypsin based protein/DNA co-extraction method applied to contact traces would yield comparable results to a commercial Proteinase K method (QIAamp DNA Investigator Kit) and a Chelex extraction method with Tween 20 used in forensic laboratories. This was tested on 20 sets of sebaceous fingerprints on glass slides.

The results of this study demonstrate the trypsin co-extraction method yielded the highest amount of DNA. In the first comparison, the mean total DNA yields for the trypsin-co extraction method and …

Discrimination Of Monozygotic Twins Using Dna Methylation Levels Of One Cpg Site At Chromosome 3, Dino O. Robinson

Student Theses

Conventional STR typing, commonly used in forensics for human identification, poses a problem in criminal cases and paternity disputes involving monozygotic (MZ) twins because they share identical DNA sequences. To date, no routine method is available in forensics to differentiate between individuals of MZ pairs. Recently, epigenetic methods measuring differential DNA methylation patterns have been applied to MZ twin differentiation. In this study, we investigated the potential to identify MZ twins using a previously identified DNA methylation site in chromosome 3, cg18562578, in a sample of 129 MZ and 37 dizygotic (DZ) twin pairs. We used bisulfite converted saliva DNA …

Potential Applications Of Microrna Profiling To Forensic Investigations, Claire L. Glynn

Forensic Science Publications

Within the forensic science community, there is a continued push to develop novel tools to aid in criminal investigations. microRNA (miRNA) analysis has been the focus of many researcher’s attention in the biomedical field since its discovery in 1993; however, the forensic application of miRNA analysis has only been suggested within the last 10 years and has been gaining considerable traction recently. The primary focus of the forensic application of miRNA analysis has been on body fluid identification to provide confirmatory universal analysis of unknown biological stains obtained from crime scenes or evidence items. There are, however, other forensic applications …

The Forensic Analysis Of Children's Temporary Tattoos, Erika Bravo

Student Theses

While children’s temporary tattoos have been produced since the 20th century, there are few scientific studies that have evaluated their physical and chemical characteristics. Therefore, this thesis is a preliminary study done to evaluate the composition of children’s temporary tattoos and consider the importance of such traces in a forensic content.

Colored regions in children’s temporary tattoos, such as red, pink, yellow and orange, were found to fluoresce with the use of an alternative light source while the use of microscopy led to the identification of different printing patterns between temporary tattoos. However, limitations in the ability to determine the …

Investigating The Isolation And Amplification Of Micrornas For Forensic Body Fluid Identification, Kelsie R. O'Leary, Claire L. Glynn

Forensic Science Publications

Background: The discovery of forensic DNA typing evolved molecular biology far beyond what could have been expected in terms of its forensic application, and now there exists other developments in molecular biology which are ready for application to forensic challenges. One such challenge is the identification of the body fluid source of stains recovered from evidence items and crime scenes. Currently there are significant efforts in the research field to develop novel methods for the molecular identification of body fluids, with microRNAs (miRNAs) revealing great potential. MiRNAs have been shown to have high tissue specificity and are less susceptible to …

Oil Dries: A New Absorbent Material For Collection Of Ignitable Liquids From Semi-Porous Material, Jessica Sosa

Online Theses and Dissertations

Extraction of an ignitable liquid sample from a semi-porous surface such as concrete is especially difficult. For this purpose, two different solid oil dry absorbent materials, Instazorb and MAXXAbsorb, were researched for use of collection of ignitable liquid samples at a fire scene. These oil dries were spiked with different ignitable liquids (Coleman fuel, gasoline, kerosene, and diesel). Isolation of the ignitable liquid from the oil dry absorbent was accomplished by passive headspace isolation. Data was obtained by gas chromatography coupled with flame ionization detection (GC-FID) and gas chromatography coupled with mass spectrometry (GC-MS). Chromatograms obtained from both oil dries …

Science Maps Of Global And Indian Wildlife Forensics: A Comparative Analysis, John Jeyasekar Jesubright, Saravanan P

Library Philosophy and Practice (e-journal)

Science map is a useful tool to understand the structure of a discipline, research networks and collaborations. Wildlife forensics is an emerging field of Forensic Sciences, where science is applied to legal cases involving wildlife. This study is aimed at creating science maps of Wildlife Forensics, both at global level and regional (i.e. India) level using PubMed database. A total of 303 records pertaining to global and 29 records pertaining to India published between 2001 and 2015 are obtained from the PubMed. These bibliometric data are analysed and maps are constructed using MS-Excel spreadsheets, VOSviewer and Pajek software. The …

Analysis Of Mitochondrial Dna Coding Region Snps By Pyrosequencing, Kyle Robert Carl Parker

Electronic Theses and Dissertations

To date, the use of mitochondrial DNA in forensic analysis has relied on the presence of variations in the control region to differentiate between samples. One problem that this analysis has shown is the occurrence of common Haplogroup H haplotypes or identical sequences. Thus, there is a need to enhance the distinguishing power of this type of analysis. One option has been to investigate the mitochondrial coding region for polymorphisms that could differentiate between samples with identical control region haplotypes. The goal of this study has been to identify polymorphic coding region sites for development in a Pyrosequencing assay that …