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Articles 1 - 25 of 25
Full-Text Articles in Forensic Science and Technology
An Internal Validation Of The Ande Rapid Dna Instrument For Bone, Tissue, And Blood Samples, Alexandra Leichnam
An Internal Validation Of The Ande Rapid Dna Instrument For Bone, Tissue, And Blood Samples, Alexandra Leichnam
Student Theses
The goal of this study is to internally validate the ANDE 6C Rapid DNA I-Chip reagent for use on disaster victim identification (DVI) samples. The internal validation tested nine samples each of postmortem blood, tissue, and bone. This study tested the instrument’s precision, accuracy, repeatability, and reproducibility. Precision and accuracy were tested by confirming that the same sample run on the ANDE instrument and the Power Plex Fusion amplification system produced a DNA profile that is consistent with the known profile. Repeatability was tested through confirming the correct results are obtained for sample runs performed by the same operator. Reproducibility …
A Comparative Analysis Of Swabbing Solutions For Sexual Assault Evidence: Direct Impact On Spermatozoa And Dna Recovery, Olivia Q. Kassie
A Comparative Analysis Of Swabbing Solutions For Sexual Assault Evidence: Direct Impact On Spermatozoa And Dna Recovery, Olivia Q. Kassie
Master of Science in Forensic Science Directed Research Projects
Forensic laboratories struggle with a backlog of sexual assault cases, prompting the need for effective techniques to develop consistent protocols and address scientific gaps. Challenges in recovering optimal DNA from assault evidence stem from factors like collection timing and sampling variables. Variations in lab protocols can lead to differences in DNA yields and profile success. This was observed in a state lab when protocols were developed allowing the use of acetone to swab sexual assault evidence. The use of acetone originated due to their case-routing protocols in multi-section cases involving latent fingerprints. While not necessary for sexual assault and porous …
Quantitation And Visualization Of Epithelial Cells On Cartridge Cases, Paige Kibler, Christopher Ehrhardt, Katherine M. Philpott, Jody H. West, Stephanie Walcott
Quantitation And Visualization Of Epithelial Cells On Cartridge Cases, Paige Kibler, Christopher Ehrhardt, Katherine M. Philpott, Jody H. West, Stephanie Walcott
Master of Science in Forensic Science Directed Research Projects
Fired cartridge cases are a common type of evidence submitted to forensic DNA profiling but are challenging to analyze because they usually contain only trace amounts of biological material. As such, there is a significant need in caseworking units for new screening techniques to determine if biological material is present and is in high enough quantity to attempt DNA profiling. The goal of this study was to test a new method for quickly visualizing biological material on cartridge cases. Biological deposits were created by handling fired and unfired cartridge cases through a series of conditions. The biological material was collected …
The Evaluation Of Semen Extraction Efficiency And Recovery From Reusable Menstrual Underwear, Caitlyn Spencer
The Evaluation Of Semen Extraction Efficiency And Recovery From Reusable Menstrual Underwear, Caitlyn Spencer
Master of Science in Forensic Science Directed Research Projects
A new type of undergarment and feminine product is being submitted to crime laboratories as evidence for sexual assaults: menstrual underwear is underwear that have absorbency and leak-proof abilities and can be washed and re-worn multiple times. The goal of this research was to determine if semen and sufficient DNA could be recovered from reusable menstrual underwear and used to obtain quality DNA profiles. Three menstrual underwear brands of Hanes, Knix, and Thinx were evaluated. Simulated post-coital samples were deposited onto three pairs of each brand of underwear. One pair of each brand was analyzed unwashed, another set was washed …
Comparing Bone Histology, Topography, And Other Physical Attributes To The Presence Of Viable Dna After Interval Cremation, Eldad Cano Jr
Comparing Bone Histology, Topography, And Other Physical Attributes To The Presence Of Viable Dna After Interval Cremation, Eldad Cano Jr
Student Theses
This research aims to compare the resulting characteristics caused by thermal exposure to bone samples – such as physical composition, microscopic histology, and scanning electron microscopy (SEM) analysis – to the qualitative and quantitative DNA analysis within the samples. By using kilns to cremate sus scrofa domesticus bone samples at five different temperatures, cortical and cancellous bones of the tibia, humerus, femur, and fibula will be analyzed and compared to determine any correlation with these factors and the presence of viable DNA. This contribution to research within the realm of forensic anthropology can grant a better understanding of how bone …
Dna Shedding Propensity And Individual Characteristics, Genesis Echavarria
Dna Shedding Propensity And Individual Characteristics, Genesis Echavarria
Student Theses
This study attempted to correlate individual biological characteristics to the amount of DNA recovered from a person’s hands (representative of shedding propensity). We used adhesive D-Squame tape to collect DNA from the fingers of more than 100 volunteers with and without controlled activity after handwashing. Two no-activity samples were taken after 30 min from the non-dominant hand and two samples from the dominant hand were collected after 60 min of controlled activity. Involuntary touches during the 60-min activity period were counted. All samples were extracted for DNA, quantitated, and genotyped to detect mixtures. Skin measurements for hydration, sebum, and melanin …
Examination Of Kastle-Meyer’S Limit Of Detection And Cross Reactivity, Djonnique M. Devone
Examination Of Kastle-Meyer’S Limit Of Detection And Cross Reactivity, Djonnique M. Devone
Forensic Science Master's Projects
Forensic Serology is a branch of forensic science that deals with the identification of certain trace evidence. Blood is the most commonly recovered and most important piece of evidence analyzed and used in criminal investigations. As evidence blood holds significance in criminal investigations because it can link a crime to an individual or exclude their involvement. Serology and DNA analysis are often aligned, because the same evidence item is often analyzed by both sections. During a criminal investigation it is important for to identifying the type of biological material or trace evidence found. The possible identification of bodily fluids can …
Development Of A Non-Invasive Dna Extraction Method From Teeth, Monalisa Heng
Development Of A Non-Invasive Dna Extraction Method From Teeth, Monalisa Heng
Honors Theses
Forensic dentistry has evolved from simple morphological investigations to genetic and molecular analysis. One of the most important techniques is DNA analysis which requires viable samples from an individual or crime site. The orofacial region can be a good source for DNA samples because the tissues can resist environmental, thermal, electrical, and mechanical challenges. Teeth are especially useful as a source of genetic material because the DNA located in the pulp is surrounded by protective mineral tissue. The DNA in the pulp tissue is currently accessed through destructive methods involving crushing, pulverization, and splitting the tooth. Unfortunately, destructive methods destroy …
Bci Validation: Yfiler Plus Kit Validation, Chloe Koon
Bci Validation: Yfiler Plus Kit Validation, Chloe Koon
Honors Projects
In order to have access and use the Combined DNA Index System (CODIS), it is required by the Federal Bureau of Investigation's Quality Assurance Standards (FBI-QAS) for Forensic DNA Units to complete comprehensive validation studies on all equipment, materials, and methods used in the process of DNA analysis. This study continues validation previous done within the Ohio Bureau of Criminal Investigation (BCI). YFiler Plus is a DNA amplification kit that is used in cases where it is most useful to examine only male DNA profile(s) within a sample by amplifying loci only on the Y-Chromosome. Components of this study include …
Reproducibility Of Individual Dna Deposits Detected Through Cellular Fluorescence, Natalee Small-Davidson
Reproducibility Of Individual Dna Deposits Detected Through Cellular Fluorescence, Natalee Small-Davidson
Student Theses
Contact traces are an important part of DNA casework, but the probative value of any identified associations depends on the possibility of passive transfer. There is known individual variation in DNA left behind during contact, this DNA shedding propensity has an effect on whose DNA is detected. This study evaluated this variability using a cell staining approach. Volunteers were asked to deposit a fingerprint on a clean glass slide, then wash their hands and deposit a second fingerprint after a 30-minute wait without touching anything. Three sets of samples were collected over three consecutive weeks. Fingerprints were stained with a …
Dna Extraction And Genotyping From Burned Skeletal Remains, Kayla M. Rooney
Dna Extraction And Genotyping From Burned Skeletal Remains, Kayla M. Rooney
Student Theses
In this research, bovine leg bones were burned, both with and without meat intact, in order to determine if it was possible to extract and amplify DNA to obtain profiles. The meat was burned over an open flame and the DNA was extracted using the Qiagen DNeasy Blood & Tissue Mini Kit, quantified with the NanoDrop, and amplified using the Bovine Genotypes Panel 3.1 Kit, and separated using the 3500 Genetic Analyzer. Profiles were analyzed using the Thermo Fisher Cloud Microsatellite Analysis software. The samples burned with the meat intact produced lower quantities of DNA than the bones burned with …
Correlation Between Tooth Root Translucency, Crystallinity, And Dna Preservation In Teeth For Unidentified Human Remains (Uhr) Investigations, Paul Yount
Master's Theses
Endogenous genetic material protected by the rigid, mineralized hard tissues of bones and teeth is often targeted for forensic DNA testing in missing persons and unidentified human remains (UHR) investigations. Although standard approaches have narrowed sample selection to a few optimal skeletal elements (i.e., weight-bearing long bones, teeth), subsampling and decisions beyond this can be challenging due to the non-uniform, heterogeneous pattern of postmortem diagenesis. One particular component of skeletal microstructure — the inorganic mineral matrix [hydroxyapatite (HAp), Ca10(PO4)6(OH)2] — is purported to play a major role in DNA preservation. The potential correlation between molecular changes to skeletal microstructure and …
The Absence Or Misuse Of Statistics In Forensic Science As A Contributor To Wrongful Convictions: From Pattern Matching To Medical Opinions About Child Abuse, Keith A. Findley
The Absence Or Misuse Of Statistics In Forensic Science As A Contributor To Wrongful Convictions: From Pattern Matching To Medical Opinions About Child Abuse, Keith A. Findley
Dickinson Law Review (2017-Present)
The new scrutiny that has been applied to the forensic sciences since the emergence of DNA profiling as the gold standard three decades ago has identified numerous concerns about the absence of a solid scientific footing for most disciplines. This article examines one of the lesser-considered problems that afflicts virtually all of the pattern-matching (or “individualization”) disciplines (largely apart from DNA), and even undermines the validity of other forensic disciplines like forensic pathology and medical determinations about child abuse, particularly Shaken Baby Syndrome/Abusive Head Trauma (SBS/AHT). That problem is the absence or misuse of statistics. This article begins by applying …
Further Evaluation Of A Dry Vacuuming Technique For Recovery Of Dna From Handwritten Documents, Christian Hopkins
Further Evaluation Of A Dry Vacuuming Technique For Recovery Of Dna From Handwritten Documents, Christian Hopkins
Student Theses
A previously developed, non-destructive, homemade vacuum method for collecting biological material from handwritten documents had promising DNA results for white copy paper, preserved indented writing, and latent prints. Prior to casework implementation, additional validation experiments are warranted and here the method was tested for different paper substrates. This work describes testing of notebook paper, bank deposit slips, magazine pages, and manila envelopes. The quantity of recovered DNA varied from donor to donor, but the mean quantities showed a trend that can be explained by the different sizes and surface properties of the tested paper types. The rougher paper type, like …
Developing A Maceration Method For Isolation Of Dna From Cancellous Bones, Bryan Bernal
Developing A Maceration Method For Isolation Of Dna From Cancellous Bones, Bryan Bernal
Student Theses
Prior to isolating DNA, it is necessary to remove any biological materials that may be present on commingled remains. Manual maceration of the outer surface is time-consuming. This study will address this issue by developing a simple method for processing bone samples prior to DNA isolation. A liquid-based technique was applied to macerate bone fragments by incubating bone fragments in an enzyme solution, removing potential contaminants, followed by a bone tissue disruption. Swine (Sus scrofa) bones were used to simulate human bones. Microscopic analysis suggested this method was effective for surface material removal. The methods effect on the …
Dna Mixture Statistics Using A Likelihood Ratio Software Tool: Effect Of Variations In Drop-Out Rates And Number Of Contributors, Felicia M. Lucero
Dna Mixture Statistics Using A Likelihood Ratio Software Tool: Effect Of Variations In Drop-Out Rates And Number Of Contributors, Felicia M. Lucero
Student Theses
Complex DNA mixtures can be very probative evidence, but comparisons to a person of interest can be affected by allelic drop-out and uncertainty regarding the number of individuals having contributed DNA to a sample. Scientific organizations such as the International Society of Forensic Genetics (Gill et al., 2006) recommend that likelihood ratios should be used to provide a statistical weight when a positive association is made between the DNA profile of a person of interest and an evidentiary DNA sample. To this effect the New York City Office of Chief Medical Examiner (OCME) developed a software program, Forensic Statistical Tool …
Catching Killers With Consumer Genetic Information, Angela Hackstadt
Catching Killers With Consumer Genetic Information, Angela Hackstadt
University Libraries Faculty Scholarship
In April 2018, Joseph James D'Angelo was arrested as a suspect in the Golden State Killer case. DNA evidence collected at a 1980 crime scene finally shed light on the murderer's identity in early 2018 when investigators turned to GEDMatch, a service that allows users to upload and share DNA data obtained from consumer genetic tests. Consumer genetic testing, DNA collection, and familial DNA searching all raise ethical and privacy concerns. If investigators are using genetic genealogy to solve cold cases, where does that leave consumers?
Haplotype Analysis For Irish Ancestry, Heather Miller Coyle, Robert Whiting
Haplotype Analysis For Irish Ancestry, Heather Miller Coyle, Robert Whiting
Forensic Science Publications
Forensic haplotype analysis of the male Y chromosome is currently used to establish the number of male donors in sexual assaults, the number of male bleeders in blood pattern analysis, and for ancestry correlation to genetic founder populations in biogeographic studies. In forensic laboratory applications, its primary use is for DNA profile generation with trace amounts of male DNA in the presence of excess female DNA (e.g. spermatozoa identification, male component of fingernail scrapings). Our study supports the potential use of the Y chromosome in a “dragnet” approach (most haplotypes are unique) similar to that described by Kayser in 2017 …
Dna Recovery From Handwritten Documents Using A Novel Vacuum Technique, Patrick M. Mclaughlin
Dna Recovery From Handwritten Documents Using A Novel Vacuum Technique, Patrick M. Mclaughlin
Student Theses
Investigations of many crimes such as robberies, kidnappings, and terrorism are often associated with the recovery of a paper document which has been written by the perpetrator. Paper can provide a variety of forensic evidence such as DNA, latent fingermarks, and indented writing. The focus of this study was the collection of probative DNA profiles from the text region of a handwritten note through a vacuum suction device without altering or destroying the document. Collection of DNA evidence was carried out in two separate groups. The first group involved 11 volunteers providing a handwritten note sample with unwashed hands. The …
Investigating The Isolation And Amplification Of Micrornas For Forensic Body Fluid Identification, Kelsie R. O'Leary, Claire L. Glynn
Investigating The Isolation And Amplification Of Micrornas For Forensic Body Fluid Identification, Kelsie R. O'Leary, Claire L. Glynn
Forensic Science Publications
Background: The discovery of forensic DNA typing evolved molecular biology far beyond what could have been expected in terms of its forensic application, and now there exists other developments in molecular biology which are ready for application to forensic challenges. One such challenge is the identification of the body fluid source of stains recovered from evidence items and crime scenes. Currently there are significant efforts in the research field to develop novel methods for the molecular identification of body fluids, with microRNAs (miRNAs) revealing great potential. MiRNAs have been shown to have high tissue specificity and are less susceptible to …
Beyond Dna: Epigenetics And Proteomics In Forensic Science, Diane F. Eilerts
Beyond Dna: Epigenetics And Proteomics In Forensic Science, Diane F. Eilerts
Themis: Research Journal of Justice Studies and Forensic Science
The use of genetic evidence in criminal cases is well established and has improved the public opinion and credibility of forensic science. However, several shortcomings associated with current genetic profiling techniques exist. Scientific research aimed at increasing the overall knowledge and understanding of biological factors will lead to the development of methods capable of improving the discriminating power of DNA evidence, overcoming limitations associated with DNA evidence, or complementing current methods of DNA profiling. Increased research in the fields of epigenetics and proteomics are particularly promising and relevant to forensic science. Research suggests that epigenetic biomarkers can be used to …
Touch Dna In A Complicated Alleged Child Abuse Case, Heather Miller Coyle
Touch Dna In A Complicated Alleged Child Abuse Case, Heather Miller Coyle
Forensic Science Publications
Touch DNA can be of use in establishing what may have occurred through reconstruction of events based on biological evidence transfer. However, interpretation of results and patterns must be approached with some caution as in the alleged child abuse case detailed here. This case was brought forward as a touch DNA and body fluid case where the male in question was a father reported to have forced a young child to perform oral sex on him. Her pajamas were collected and evaluated for presence of body fluids and associated DNA. The sleeves of the pajamas tested positive for amylase, a …
Comparing Wearer Dna Sample Collection Methods For The Recovery Of Single Source Profiles, Corissa J. Harris, Amanda J. Cardenas, Steven B. Lee, Brooke Barloewen
Comparing Wearer Dna Sample Collection Methods For The Recovery Of Single Source Profiles, Corissa J. Harris, Amanda J. Cardenas, Steven B. Lee, Brooke Barloewen
Themis: Research Journal of Justice Studies and Forensic Science
Wearer DNA is the deposit of epithelial cells on clothing worn by an individual. Detection of the last individual to handle or wear an item is often an important and desirable determination in forensic science. The most commonly used collection methods for wearer DNA include swabbing and scraping. These often result in mixture profiles. Recently, adhesives have been introduced as a possible reliable method for the collection of biological evidence. The goal of the research was to compare the current collection methods of swabbing and scraping with a gel film called Gel-Pak ‘0’ which shares similar properties with adhesives. Gel-Pak …
Field Testing Of Collection Cards For Cannabis Sativa Samples With A Single Hexanucleotide Dna Marker, Lindsey Allgeier, John Hemenway, Nicholas Shirley, Tommy Lanier, Heather Miller Coyle
Field Testing Of Collection Cards For Cannabis Sativa Samples With A Single Hexanucleotide Dna Marker, Lindsey Allgeier, John Hemenway, Nicholas Shirley, Tommy Lanier, Heather Miller Coyle
Forensic Science Publications
Abstract: The validity and feasibility of using DNA collection cards in the field for preservation and analysis of Cannabis sativa genotypes were investigated using a highly specific hexanucleotide marker. Collection cards were submitted to the National Marijuana Initiative, which selectively trained and managed the collection of specific types of samples from a variety of participating agencies. Samples collected at seizure sites included fresh marijuana leaf samples, dried “dispensary” samples, U.S. border seizures, and hashish. Using a standardized PCR kit with custom-labeled oligonucleotide primers specific to marijuana, collection cards produced eight genotypes and 13 different alleles, extremely low baselines, and no …
The Relative Recoverability Of Dna And Rna Profiles From Forensically Relevant Body Fluid Stains, Charly Parker
The Relative Recoverability Of Dna And Rna Profiles From Forensically Relevant Body Fluid Stains, Charly Parker
Electronic Theses and Dissertations
Biological material (fluids or tissues) whether from the victim or suspect is often collected as forensic evidence, and methods to obtain and analyze the DNA found in that material have been well established. The type of body fluid (i.e. blood, saliva, semen, vaginal secretions, and menstrual blood) from which the DNA originated is also of interest, and messenger RNA typing provides a specific and sensitive means of body fluid identification. In order for mRNA profiling to be utilized in routine forensic casework, RNA of sufficient quantity and quality must be obtained from biological fluid stains and the methods used for …