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Articles 1 - 10 of 10
Full-Text Articles in Forensic Science and Technology
Method Optimization For The Determination Of Cannabinoids In Blood By Liquid Chromatography Tandem Mass Spectrometry (Lc-Ms/Ms), Kevin D. Carrera
Method Optimization For The Determination Of Cannabinoids In Blood By Liquid Chromatography Tandem Mass Spectrometry (Lc-Ms/Ms), Kevin D. Carrera
Student Theses
Delta-9-tetrahydrocannabinol (THC) continues to be one of the most popular drugs in the USA. Along with THC, other cannabinoids such as cannabidiol (CBD), are on the rise due to an increase in medicinal usage as well as the passage of different legislations removing these products from their current schedule I status. Thus, an urgency exists to develop robust and sensitive analytical methods to determine cannabinoids, especially THC, CBD and metabolites, in biological samples. The purpose of this study was to investigate different analytical procedures to determine the best method to identify and quantify CBD, THC and their metabolites in whole …
Development Of A Loop-Mediated Isothermal Amplification (Lamp) Assay For Detection Of Mirnas, Alisha B. Whelan
Development Of A Loop-Mediated Isothermal Amplification (Lamp) Assay For Detection Of Mirnas, Alisha B. Whelan
Master of Science in Forensic Science Directed Research Projects
Body fluid identification serves a vital role in contextualizing biological evidence received in forensic laboratories. While several fluids can be presumptively detected using immunological or catalytic tests, these tests are susceptible to false results and have limited sensitivity. True confirmatory testing is still somewhat limited in forensic laboratories. With the advancement of DNA typing technologies, laboratories can develop DNA profiles from trace amounts of sample, increasing the demand for sensitive and accurate body fluid detection. This project proposes using loop-mediated isothermal amplification (LAMP) as a method to rapidly detect microRNAs (miRNAs) that are differentially expressed in various body fluids with …
Cocaine, Cocaethylene And Ethanol In Postmortem Blood Submitted To The New York City Office Of Chief Medical Examiner, Jada T. Alexis
Cocaine, Cocaethylene And Ethanol In Postmortem Blood Submitted To The New York City Office Of Chief Medical Examiner, Jada T. Alexis
Student Theses
Cocaine overdose deaths are on the rise in the United States. This increase may be due to the co-consumption of cocaine with other drugs. When cocaine is ingested in conjunction with alcohol, a specific metabolite called cocaethylene forms. Information regarding cocaethylene, specifically its interpretive value in post-mortem cases, has not been widely studied. Data from the Office of the Chief Medical Examiner in New York City revealed that there were 195 cases in 2017 positive for cocaethylene. These cases were most likely to involve black (42.1%) males (79.5%) in Manhattan (28.2%) aged 50 - 59 (34.4%). Most of the cases …
Qualitative Analysis Of Luminol Efficacy On Bleach-Cleaned And Paint-Concealed Blood, Adam Wykoff
Qualitative Analysis Of Luminol Efficacy On Bleach-Cleaned And Paint-Concealed Blood, Adam Wykoff
Themis: Research Journal of Justice Studies and Forensic Science
The presence of blood at a crime scene can provide investigators with a treasure trove of information regarding the nature and circumstances of a particular crime and aid in crime scene reconstruction; however, attempts at concealing blood are common scenarios. The development of chemiluminescent and fluorescent-based presumptive tests, such as the luminol and fluorescein tests, have made it more challenging to definitively remove or mask blood on a surface. The purpose of this experiment was to qualitatively measure the overall efficacy of luminol, concerning its ability to positively detect small bloodstains found on common household floor surfaces (wood, carpet, and …
Analysis Of Cannabinoids In Serum By Gc-Ms/Ms, Christie Cannarozzi
Analysis Of Cannabinoids In Serum By Gc-Ms/Ms, Christie Cannarozzi
Student Theses
Due to recent changes in federal and state legislations, the availability and consumption of cannabis products have increased in the United States. The expanded use of recreational and medicinal cannabis products increases the importance of implementing sensitive and selective instrumental methods in toxicological laboratories, as legal implications may arise in forensic cases, such as driving under the influence of drugs (DUID). The purpose of this study was to perform a cross-validation for the quantitative analysis of cannabinoids (Δ9-tetrahydrocannabinol, cannabidiol, cannabinol, 11-hydroxy-Δ9-tetrahydrocannabinol, and 11-nor-9-carboxy-Δ9-tetrahydrocannabinol) in serum by gas chromatography coupled to triple quadrupole tandem mass spectrometry (GCTQ). This method was fully …
Method Development And Validation For The Determination Of Designer Benzodiazepines In Blood By Lc-Ms/Ms, Victoria Mei
Method Development And Validation For The Determination Of Designer Benzodiazepines In Blood By Lc-Ms/Ms, Victoria Mei
Student Theses
The misuse of designer benzodiazepines, as an alternative to prescription benzodiazepines and for drug-facilitated sexual assaults, has emerged as a growing threat, due in part to the ease of purchasing these drugs on the internet at low prices. Causing concern for safety, is the lack of dosage information resulting in users self-medicating, often leading to unintended overdoses, coma, or death at higher doses. With limited published data regarding quantification of designer benzodiazepines in forensic cases, a method was validated for the determination of thirteen and screening for two designer benzodiazepines in blood, in addition to a limited number of common …
Analysis Of Synthetic Opioids In Postmortem Blood, Vitreous Humor, And Brain Tissue, Rachel K. Chesser
Analysis Of Synthetic Opioids In Postmortem Blood, Vitreous Humor, And Brain Tissue, Rachel K. Chesser
Student Theses
In the United States, the use of new synthetic opioids (e.g. fentanyl and derivatives) has become an increasing health issue with thousands of overdose deaths being observed since 2013. With the high mortality rate associated with these substances, postmortem analyses and interpretation of synthetic opioids has become extremely important. However, due to the novelty of these compounds, the available data is limited and provides challenges to toxicologists. The focus of this project was to examine the postmortem distribution of new synthetic opioids in blood, vitreous humor, and brain tissue. New methods were developed and validated to quantify 13 synthetic opioids …
A Comparative Analysis Of Protein And Peroxidase Blood Enhancement Reagents Following Laundering And Their Impact On Dna Recovery, Gabrielle Hartley, Claire L. Glynn
A Comparative Analysis Of Protein And Peroxidase Blood Enhancement Reagents Following Laundering And Their Impact On Dna Recovery, Gabrielle Hartley, Claire L. Glynn
Forensic Science Publications
Blood is a commonly encountered biological fluid in criminal investigations concerning a violent incident, and visual traces of the fluid on a suspect’s clothing can be diminished through laundering. This study aims to analyze the effects of laundering and the application of commercially available blood enhancement reagents commonly used to improve visualization of dilute bloodstains and their impact on DNA recovery. Enhancement reagents Hungarian Red, Coomassie Blue, Amido Black, luminol, Bluestar® Forensic Magnum, and aqueous Leuco Crystal Violet (LCV) were used to enhance human blood on cotton, polyester, denim, and wool following laundering. DNA was extracted from these samples using …
A Validation Study Of Zar-Pro Fluorescent Blood Lifting Strips, Carter L. Depew
A Validation Study Of Zar-Pro Fluorescent Blood Lifting Strips, Carter L. Depew
Honors Theses
It is well known within the latent fingerprint discipline that collection of bloody impressions can be difficult and destructive. This pilot study aims to validate the use of Zar-Pro Fluorescent Blood Lifting Strips© in the collection of bloody fingerprint impressions, and then compare the technique outcomes that of the currently used method – photography. This study used both collection methods to extract bloody impressions from white copy paper and aluminum metal. The impressions were aged over a two-week period prior to collection. A numerical score – representative of the identifiable minutiae points – was then obtained using the Smart Extract …
Field Technique For The Identification Of Deer Blood, David W. Oates, Carol A. Jochum, Kenneth A. Pearson, Cathy A. Hoilien
Field Technique For The Identification Of Deer Blood, David W. Oates, Carol A. Jochum, Kenneth A. Pearson, Cathy A. Hoilien
Nebraska Game and Parks Commission: Staff Research Publications
A latex suspension sensitized with deer antiserum has been prepared, placed on plastic cards, dried, and packaged for field use. The product was tested against bloodstains from 22 species (including Homo sapiens). Strong agglutination reactions occurred only with bloodstains from deer and elk.